################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 403 GTAGAC GT^[AC][GT]AC AfaI 333 GTAC GT^AC AflIII 524 ACACGT A^C[GA][CT]GT AocI 241 CCTGAGG CC^T[ACGT]AGG AvaI 185 CCCGGG C^[CT]CG[GA]G AvaII 51 GGACC G^G[AT]CC BbvCI 588 CCTCAGC CCTCAGC(-5/-2) BciVI 439 GTATCC GTATCC(6/5) BglII 548 AGATCT A^GATCT BmyI 608 GTGCCC G[AGT]GC[ACT]^C BpmI 89 CTCCAG CTGGAG(16/14) BsaAI 436 TACGTA [CT]AC^GT[GA] BsgI 250 GTGCAG GTGCAG(16/14) BsoBI 185 CCCGGG C^[CT]CG[GA]G Bsp1286I 608 GTGCCC G[AGT]GC[ACT]^C BspHI 75 TCATGA T^CATGA BsrBI 160 GAGCGG CCGCTC(-3/-3) BsrFI 53 ACCGGC [GA]^CCGG[CT] BsrI 71 CCAGT ACTGG(1/-1) BssHII 168 GCGCGC G^CGCGC BstAPI 107 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 444 CATCC GGATG(2/0) BstYI 548 AGATCT [GA]^GATC[CT] Bsu36I 241 CCTGAGG CC^T[ACGT]AGG Cfr10I 53 ACCGGC [GA]^CCGG[CT] CpoI 50 CGGACCG CG^G[AT]CCG EarI 352 CTCTTC CTCTTC(1/4) Eco81I 241 CCTGAGG CC^T[ACGT]AGG EcoT14I 455 CCAAGG C^C[AT][AT]GG FokI 444 CATCC GGATG(9/13) HphI 462 GGTGA GGTGA(8/7) Ksp632I 352 CTCTTC CTCTTC(1/4) MflI 548 AGATCT [GA]^GATC[CT] MslI 555 CACGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PflMI 393 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 75 TCATGA T^CATGA RsaI 333 GTAC GT^AC RsrII 50 CGGACCG CG^G[AT]CCG SapI 351 GCTCTTC GCTCTTC(1/4) SmaI 185 CCCGGG CCC^GGG SnaBI 436 TACGTA TAC^GTA StuI 179 AGGCCT AGG^CCT StyI 455 CCAAGG C^C[AT][AT]GG TaqI 530 TCGA T^CGA TthHB8I 530 TCGA T^CGA Van91I 393 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 389 CCAACCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 548 AGATCT [GA]^GATC[CT] XmaCI 185 CCCGGG C^CCGGG XmaI 185 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BstNI 149 CCTGG CC^[AT]GG 468 CCTGG DpnII 30 GATC ^GATC 549 GATC EcoRII 149 CCTGG ^CC[AT]GG 468 CCTGG HgaI 299 GACGC GACGC(5/10) 535 GACGC HinfI 472 GACTC G^A[ACGT]TC 571 GACTC MaeII 437 ACGT A^CGT 526 ACGT MboI 30 GATC ^GATC 549 GATC MboII 501 GAAGA GAAGA(8/7) 353 TCTTC MvaI 149 CCTGG CC^[AT]GG 468 CCTGG NdeII 30 GATC ^GATC 549 GATC NspI 347 ACATGC [GA]CATG^[CT] 396 GCATGT PleI 472 GACTC GAGTC(4/5) 571 GACTC PvuII 213 CAGCTG CAG^CTG 541 CAGCTG Sau3AI 30 GATC ^GATC 549 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AccII AciI AluI BbvI BcnI BsaJI BseRI BsiYI BslI BsmFI BssKI BstUI Cac8I CfoI Cfr13I DdeI FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MvnI MwoI NciI NlaIII NlaIV PstI Sau96I ScrFI SfaNI SfcI TseI