(Circular) (Six-base) MAPSORT of: pFN21AE1527.seq Check: 4411 from: 1 to: 5356 pFN21AE1527 5356 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3166 278 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2409 2699 1937 Size: 472 290 4594 Fragments arranged by size: 4594 472 290 Acc65I G'GTAC_C Cuts at: 2677 2677 Size: 5356 AclI AA'CG_TT Cuts at: 1563 3483 3856 1563 Size: 1920 373 3063 Fragments arranged by size: 3063 1920 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2402 3419 4803 5071 1632 Size: 770 1017 1384 268 1917 Fragments arranged by size: 1917 1384 1017 770 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5159 AflIII A'CryG_T Cuts at: 1203 2529 4255 5232 1203 Size: 1326 1726 977 1327 Fragments arranged by size: 1726 1327 1326 977 AgeI A'CCGG_T Cuts at: 4187 4187 Size: 5356 AhdI GACnn_n'nnGTC Cuts at: 4085 4085 Size: 5356 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5356 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5356 AlwNI CAG_nnn'CTG Cuts at: 3099 4671 3099 Size: 1572 3784 ApaLI G'TGCA_C Cuts at: 3413 4569 3413 Size: 1156 4200 ApoI r'AATT_y Cuts at: 1493 2665 2891 3043 5152 1493 Size: 1172 226 152 2109 1697 Fragments arranged by size: 2109 1697 1172 226 152 AseI AT'TA_AT Cuts at: 160 1032 3910 160 Size: 872 2878 1606 Fragments arranged by size: 2878 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 AvaI C'yCGr_G Cuts at: 1870 1943 2190 2681 1870 Size: 73 247 491 4545 Fragments arranged by size: 4545 491 247 73 BamHI G'GATC_C Cuts at: 2686 3053 2686 Size: 367 4989 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2677 4132 618 Size: 325 217 627 890 1455 1842 Fragments arranged by size: 1842 1455 890 627 325 217 BanII G_rGCy'C Cuts at: 1391 2675 1391 Size: 1284 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4385 BbvCI CC'TCA_GC Cuts at: 2594 2594 Size: 5356 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3547 3581 1903 Size: 34 50 34 1526 34 3678 Fragments arranged by size: 3678 1526 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2455 3248 4458 2455 Size: 793 1210 3353 Fragments arranged by size: 3353 1210 793 BclI T'GATC_A Cuts at: 1548 1548 Size: 5356 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3967 136 Size: 107 122 71 631 605 2295 1525 Fragments arranged by size: 2295 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2553 5351 2553 Size: 2798 2558 BlpI GC'TnA_GC Cuts at: 2773 2773 Size: 5356 Bme1580I G_kGCm'C Cuts at: 1790 2617 3417 4573 1790 Size: 827 800 1156 2573 Fragments arranged by size: 2573 1156 827 800 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5356 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3087 4045 442 Size: 416 53 83 399 24 1670 958 1753 Fragments arranged by size: 1753 1670 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5356 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2079 4016 1239 Size: 840 1937 2579 Fragments arranged by size: 2579 1937 840 Bpu10I CC'TnA_GC Cuts at: 2594 2594 Size: 5356 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3481 4346 4644 3481 Size: 865 298 4193 Fragments arranged by size: 4193 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4019 882 Size: 617 2520 2219 Fragments arranged by size: 2520 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2443 3147 493 Size: 711 1239 704 2702 Fragments arranged by size: 2702 1239 711 704 BsaBI GATnn'nnATC Cuts at: 5350 5350 Size: 5356 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3163 3545 4180 275 Size: 53 83 186 936 1630 382 635 1451 Fragments arranged by size: 1630 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3789 4187 4461 4608 5019 3789 Size: 398 274 147 411 4126 Fragments arranged by size: 4126 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2077 2107 2561 2591 1178 Size: 30 165 30 674 30 454 30 3943 Fragments arranged by size: 3943 674 454 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2264 2360 2390 2635 1186 Size: 877 201 96 30 245 3907 Fragments arranged by size: 3907 877 245 201 96 30 BseYI C'CCAG_C Cuts at: 2105 2216 2262 2296 4559 5055 2105 Size: 111 46 34 2263 496 2406 Fragments arranged by size: 2406 2263 496 111 46 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2276 1163 Size: 573 540 4243 Fragments arranged by size: 4243 573 540 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2806 3567 4595 1540 Size: 275 186 805 761 1028 2301 Fragments arranged by size: 2301 1028 805 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3417 3502 4573 3417 Size: 85 1071 4200 Fragments arranged by size: 4200 1071 85 BsmI GAATG_Cn' Cuts at: 2867 2960 2867 Size: 93 5263 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2617 2675 3417 3502 4573 1391 Size: 399 827 58 742 85 1071 2174 Fragments arranged by size: 2174 1071 827 742 399 85 58 BspEI T'CCGG_A Cuts at: 5019 5019 Size: 5356 BspHI T'CATG_A Cuts at: 2080 3245 4975 2080 Size: 1165 1730 2461 Fragments arranged by size: 2461 1730 1165 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2711 844 Size: 1867 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2167 3243 1394 Size: 429 344 1076 3507 Fragments arranged by size: 3507 1076 429 344 BsrDI GCAATG_nn' Cuts at: 65 1248 3851 4025 65 Size: 1183 2603 174 1396 Fragments arranged by size: 2603 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2058 4000 4187 1700 Size: 110 248 1942 187 2869 Fragments arranged by size: 2869 1942 248 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5356 BssHII G'CGCG_C Cuts at: 1925 2173 1925 Size: 248 5108 BssSI C'ACGA_G Cuts at: 3416 4428 3416 Size: 1012 4344 BstAPI GCAn_nnn'nTGC Cuts at: 794 2118 794 Size: 1324 4032 BstBI TT'CG_AA Cuts at: 4160 4160 Size: 5356 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5356 BstYI r'GATC_y Cuts at: 1972 2553 2686 3053 3438 3455 4907 4993 5005 Size: 581 133 367 385 17 1452 86 12 Cuts at: 5005 5351 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 581 385 367 346 133 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2247 2247 Size: 5356 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4804 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4617 Fragments arranged by size: 4617 683 56 BtsI GCAGTG_nn' Cuts at: 2872 3666 3686 2872 Size: 794 20 4542 Fragments arranged by size: 4542 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5356 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5321 DraI TTT'AAA Cuts at: 2660 2660 Size: 5356 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4363 809 Size: 507 3047 1802 Fragments arranged by size: 3047 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2803 3692 5321 Size: 54 1425 50 275 18 973 889 1629 Cuts at: 5321 8 Size: 43 Fragments arranged by size: 1629 1425 973 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2803 1537 Size: 275 991 4090 Fragments arranged by size: 4090 991 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2363 3286 1353 Size: 1010 923 3423 Fragments arranged by size: 3423 1010 923 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2069 3943 4317 4463 387 Size: 1064 138 480 1874 374 146 1280 Fragments arranged by size: 1874 1280 1064 480 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2079 2402 3419 4016 4803 5071 1239 Size: 393 447 323 1017 597 787 268 1524 Fragments arranged by size: 1524 1017 787 597 447 393 323 268 EcoRI G'AATT_C Cuts at: 2665 3043 2665 Size: 378 4978 FspI TGC'GCA Cuts at: 3862 3862 Size: 5356 HaeII r_GCGC'y Cuts at: 1678 4503 1678 Size: 2825 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2075 2107 2429 2461 3997 Size: 32 135 32 503 32 322 32 1536 Cuts at: 3997 4029 4071 4103 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1536 503 322 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2700 2946 669 Size: 988 281 762 246 3079 Fragments arranged by size: 3079 988 762 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5356 HpaI GTT'AAC Cuts at: 2946 2946 Size: 5356 KpnI G_GTAC'C Cuts at: 2681 2681 Size: 5356 MfeI C'AATT_G Cuts at: 2955 2955 Size: 5356 MluI A'CGCG_T Cuts at: 5232 5232 Size: 5356 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4470 4654 1305 Size: 3165 184 2007 Fragments arranged by size: 3165 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3534 Fragments arranged by size: 3534 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2564 3314 3673 3832 518 Size: 648 1398 750 359 159 2042 Fragments arranged by size: 2042 1398 750 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2099 2220 2300 2548 2772 3449 4597 4842 Size: 493 121 80 248 224 677 1148 245 Cuts at: 4842 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 493 248 245 224 121 80 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4804 NdeI CA'TA_TG Cuts at: 387 387 Size: 5356 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5356 NotI GC'GGCC_GC Cuts at: 2803 2803 Size: 5356 NspI r_CATG'y Cuts at: 1132 1229 2356 2405 2714 4259 1132 Size: 97 1127 49 309 1545 2229 Fragments arranged by size: 2229 1545 1127 309 97 49 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5356 PciI A'CATG_T Cuts at: 4255 4255 Size: 5356 PflMI CCAn_nnn'nTGG Cuts at: 1108 2404 1108 Size: 1296 4060 PfoI T'CCnGG_A Cuts at: 2153 2153 Size: 5356 PmeI GTTT'AAAC Cuts at: 2660 2660 Size: 5356 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3425 3457 4938 4970 3425 Size: 32 1481 32 3811 Fragments arranged by size: 3811 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5356 PsiI TTA'TAA Cuts at: 2926 2926 Size: 5356 PstI C_TGCA'G Cuts at: 830 2208 2553 2628 2708 830 Size: 1378 345 75 80 3478 Fragments arranged by size: 3478 1378 345 80 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5356 PvuII CAG'CTG Cuts at: 2220 2548 2220 Size: 328 5028 RsrII CG'GwC_CG Cuts at: 2056 2056 Size: 5356 SalI G'TCGA_C Cuts at: 1936 2698 1936 Size: 762 4594 SapI GCTCTTCn'nnn_ Cuts at: 2363 2363 Size: 5356 SbfI CC_TGCA'GG Cuts at: 2708 2708 Size: 5356 ScaI AGT'ACT Cuts at: 3604 5316 3604 Size: 1712 3644 SfcI C'TryA_G Cuts at: 826 1045 2143 2204 2549 2624 2704 3839 4520 Size: 219 1098 61 345 75 80 1135 681 Cuts at: 4520 4711 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1098 681 345 219 191 80 75 61 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5356 SmaI CCC'GGG Cuts at: 1872 2192 2683 1872 Size: 320 491 4545 Fragments arranged by size: 4545 491 320 SmlI C'TyrA_G Cuts at: 820 1017 1943 3460 4361 4623 820 Size: 197 926 1517 901 262 1553 Fragments arranged by size: 1553 1517 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2443 3147 493 Size: 1950 704 2702 Fragments arranged by size: 2702 1950 704 SpeI A'CTAG_T Cuts at: 152 152 Size: 5356 SphI G_CATG'C Cuts at: 1132 2714 1132 Size: 1582 3774 SspI AAT'ATT Cuts at: 5 52 3280 5 Size: 47 3228 2081 Fragments arranged by size: 3228 2081 47 StuI AGG'CCT Cuts at: 1517 2186 1517 Size: 669 4687 StyI C'CwwG_G Cuts at: 513 1065 2460 2795 513 Size: 552 1395 335 3074 Fragments arranged by size: 3074 1395 552 335 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3383 3400 3553 3738 4183 325 Size: 1255 1803 17 153 185 445 1498 Fragments arranged by size: 1803 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2337 3602 5314 Size: 274 80 33 51 1443 360 1265 1712 Cuts at: 5314 96 Size: 138 Fragments arranged by size: 1712 1443 1265 360 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2227 2259 2295 2327 2561 2593 5316 5348 2227 Size: 32 36 32 234 32 2723 32 2235 Fragments arranged by size: 2723 2235 234 36 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5356 XbaI T'CTAG_A Cuts at: 2692 2692 Size: 5356 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2401 2401 Size: 5356 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5356 XmaI C'CCGG_G Cuts at: 1870 2190 2681 1870 Size: 320 491 4545 Fragments arranged by size: 4545 491 320 XmnI GAAnn'nnTTC Cuts at: 3485 3485 Size: 5356 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3164 276 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI