(Linear) (Six-base) MAPSORT of: KIEE1527.seq Check: 2109 from: 1 to: 651 KIEE1527.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. AccI GT'mk_AC Cuts at: 0 404 651 Size: 404 247 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 397 651 Size: 397 254 AflIII A'CryG_T Cuts at: 0 524 651 Size: 524 127 AvaI C'yCGr_G Cuts at: 0 185 651 Size: 185 466 BbvCI CC'TCA_GC Cuts at: 0 589 651 Size: 589 62 BciVI GTATCCnnnnn_n' Cuts at: 0 450 651 Size: 450 201 BglII A'GATC_T Cuts at: 0 548 651 Size: 548 103 Bme1580I G_kGCm'C Cuts at: 0 612 651 Size: 612 39 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 74 651 Size: 74 577 Bpu10I CC'TnA_GC Cuts at: 0 589 651 Size: 589 62 BsaAI yAC'GTr Cuts at: 0 438 651 Size: 438 213 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 72 102 556 586 651 Size: 72 30 454 30 65 Fragments arranged by size: 454 72 65 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 58 259 355 385 630 651 Size: 58 201 96 30 245 21 Fragments arranged by size: 245 201 96 58 30 21 BseYI C'CCAG_C Cuts at: 0 100 211 257 291 651 Size: 100 111 46 34 360 Fragments arranged by size: 360 111 100 46 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 651 Size: 271 380 Bsp1286I G_dGCh'C Cuts at: 0 612 651 Size: 612 39 BspHI T'CATG_A Cuts at: 0 75 651 Size: 75 576 BsrBI CCG'CTC Cuts at: 0 162 651 Size: 162 489 BsrFI r'CCGG_y Cuts at: 0 53 651 Size: 53 598 BssHII G'CGCG_C Cuts at: 0 168 651 Size: 168 483 BstAPI GCAn_nnn'nTGC Cuts at: 0 113 651 Size: 113 538 BstYI r'GATC_y Cuts at: 0 548 651 Size: 548 103 Bsu36I CC'TnA_GG Cuts at: 0 242 651 Size: 242 409 EarI CTCTTCn'nnn_ Cuts at: 0 358 651 Size: 358 293 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 64 651 Size: 64 587 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 74 397 651 Size: 74 323 254 Fragments arranged by size: 323 254 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 70 102 424 456 651 Size: 70 32 322 32 195 Fragments arranged by size: 322 195 70 32 32 MslI CAynn'nnrTG Cuts at: 0 559 651 Size: 559 92 MspA1I CmG'CkG Cuts at: 0 94 215 295 543 651 Size: 94 121 80 248 108 Fragments arranged by size: 248 121 108 94 80 NspI r_CATG'y Cuts at: 0 351 400 651 Size: 351 49 251 Fragments arranged by size: 351 251 49 PflMI CCAn_nnn'nTGG Cuts at: 0 399 651 Size: 399 252 PfoI T'CCnGG_A Cuts at: 0 148 651 Size: 148 503 PstI C_TGCA'G Cuts at: 0 203 548 623 651 Size: 203 345 75 28 Fragments arranged by size: 345 203 75 28 PvuII CAG'CTG Cuts at: 0 215 543 651 Size: 215 328 108 Fragments arranged by size: 328 215 108 RsrII CG'GwC_CG Cuts at: 0 51 651 Size: 51 600 SapI GCTCTTCn'nnn_ Cuts at: 0 358 651 Size: 358 293 SfcI C'TryA_G Cuts at: 0 138 199 544 619 651 Size: 138 61 345 75 32 Fragments arranged by size: 345 138 75 61 32 SmaI CCC'GGG Cuts at: 0 187 651 Size: 187 464 SnaBI TAC'GTA Cuts at: 0 438 651 Size: 438 213 StuI AGG'CCT Cuts at: 0 181 651 Size: 181 470 StyI C'CwwG_G Cuts at: 0 455 651 Size: 455 196 TatI w'GTAC_w Cuts at: 0 332 651 Size: 332 319 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 222 254 290 322 556 588 651 Size: 222 32 36 32 234 32 63 Fragments arranged by size: 234 222 63 36 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 396 651 Size: 396 255 XmaI C'CCGG_G Cuts at: 0 185 651 Size: 185 466 Enzymes that do cut: AccI AcuI AflIII AvaI BbvCI BciVI BglII Bme1580I BpmI Bpu10I BsaAI BsaXI BseRI BseYI BsgI Bsp1286I BspHI BsrBI BsrFI BssHII BstAPI BstYI Bsu36I EarI EciI Eco57MI Hin4I MslI MspA1I NspI PflMI PfoI PstI PvuII RsrII SapI SfcI SmaI SnaBI StuI StyI TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BclI BfrBI BglI BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrDI BsrGI BssSI BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmnI ZraI