################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BanII BbeI BbvCI BcgI BclI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI Hin1I HpaI KasI KpnI MamI MfeI MflI MluI MluNI MroI MscI MunI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AflII 244 CTTAAG C^TTAAG Asp700I 1049 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 784 GAGCAC G[AT]GC[AT]^C BanI 297 GGTGCC G^G[CT][GA]CC BbrPI 1086 CACGTG CAC^GTG BbsI 1070 GTCTTC GAAGAC(2/6) BciVI 171 GGATAC GTATCC(6/5) BfrI 244 CTTAAG C^TTAAG BpuAI 1070 GTCTTC GAAGAC(2/6) BsaAI 1086 CACGTG [CT]AC^GT[GA] BseRI 40 CTCCTC GAGGAG(10/8) BsgI 657 GTGCAG GTGCAG(16/14) BsiHKAI 784 GAGCAC G[AT]GC[AT]^C BsmAI 925 GTCTC GTCTC(1/5) BsmI 844 GAATGC GAATGC(1/-1) BspHI 1020 TCATGA T^CATGA BsrFI 87 GCCGGC [GA]^CCGG[CT] BstF5I 320 CATCC GGATG(2/0) Cfr10I 87 GCCGGC [GA]^CCGG[CT] DpnII 237 GATC ^GATC DraI 444 TTTAAA TTT^AAA DraII 872 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoO109I 872 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoT22I 752 ATGCAT ATGCA^T FauI 25 CCCGC CCCGC(4/6) FokI 320 CATCC GGATG(9/13) HaeII 30 GGCGCT [GA]GCGC^[CT] HgaI 1116 GACGC GACGC(5/10) HincII 1098 GTTGAC GT[CT]^[GA]AC HindII 1098 GTTGAC GT[CT]^[GA]AC HindIII 201 AAGCTT A^AGCTT KspI 26 CCGCGG CCGC^GG MboI 237 GATC ^GATC MspA1I 26 CCGCGG C[AC]G^C[GT]G NaeI 87 GCCGGC GCC^GGC NcoI 1127 CCATGG C^CATGG NdeII 237 GATC ^GATC NgoMIV 87 GCCGGC G^CCGGC NsiI 752 ATGCAT ATGCA^T NspI 754 GCATGT [GA]CATG^[CT] PleI 229 GACTC GAGTC(4/5) PmaCI 1086 CACGTG CAC^GTG PmlI 1086 CACGTG CAC^GTG PpuMI 872 AGGTCCC [GA]G^G[AT]CC[CT] RcaI 1020 TCATGA T^CATGA SacII 26 CCGCGG CCGC^GG Sau3AI 237 GATC ^GATC SexAI 528 ACCAGGT A^CC[AT]GGT SfaNI 319 GCATC GCATC(5/9) SfcI 917 CTACAG C^T[GA][CT]AG TfiI 767 GATTC G^A[AT]TC XmnI 1049 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AflIII 1085 ACACGT A^C[GA][CT]GT 1087 ACGTGT AvaI 43 CTCGGG C^[CT]CG[GA]G 166 CCCGAG AvaII 452 GGTCC G^G[AT]CC 873 GGTCC BcnI 56 CCCGG CC^[GC]GG 496 CCGGG BfaI 602 CTAG C^TAG 1120 CTAG BglI 242 GCCTTAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 300 GCCAACAAGGC BsiEI 13 CGGCCG CG[GA][CT]^CG 89 CGGCCG BsmFI 1114 GGGAC GGGAC(10/14) 874 GTCCC BsoBI 43 CTCGGG C^[CT]CG[GA]G 166 CCCGAG BsrI 358 CCAGT ACTGG(1/-1) 633 CCAGT CfoI 10 GCGC GCG^C 31 GCGC DsaI 26 CCGCGG C^C[GA][CT]GG 1127 CCATGG EaeI 13 CGGCCG [CT]^GGCC[GA] 89 CGGCCG EagI 13 CGGCCG C^GGCCG 89 CGGCCG EarI 271 GAAGAG CTCTTC(1/4) 401 GAAGAG EclXI 13 CGGCCG C^GGCCG 89 CGGCCG Eco52I 13 CGGCCG C^GGCCG 89 CGGCCG EcoT14I 869 CCAAGG C^C[AT][AT]GG 1127 CCATGG HhaI 10 GCGC GCG^C 31 GCGC HinP1I 10 GCGC G^CGC 31 GCGC HinfI 229 GACTC G^A[ACGT]TC 767 GATTC Ksp632I 271 GAAGAG CTCTTC(1/4) 401 GAAGAG MaeI 602 CTAG C^TAG 1120 CTAG MseI 245 TTAA T^TAA 445 TTAA MslI 839 CACCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1015 CATTTTCATG NciI 56 CCCGG CC^[GC]GG 496 CCGGG SmlI 244 CTTAAG C^T[CT][GA]AG 974 CTCAAG StyI 869 CCAAGG C^C[AT][AT]GG 1127 CCATGG TaqI 464 TCGA T^CGA 595 TCGA Tru9I 245 TTAA T^TAA 445 TTAA TspRI 233 CAGTG CA[GC]TG[ACGT][ACGT]^ 634 CAGTG TthHB8I 464 TCGA T^CGA 595 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AccII AciI AcsI AfaI AluI ApoI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI BstUI Cac8I Cfr13I DdeI EcoRII Fnu4HI HaeIII HapII HpaII HphI ItaI MaeII MaeIII MboII MnlI MspI MvaI MvnI MwoI NlaIII NlaIV RsaI Sau96I ScrFI TseI Tsp45I Tsp509I