(Circular) (Six-base) MAPSORT of: pFN21AE1473.seq Check: 4703 from: 1 to: 5848 pFN21AE1473 5848 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3658 278 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3191 1937 Size: 1254 4594 Acc65I G'GTAC_C Cuts at: 3169 3169 Size: 5848 AclI AA'CG_TT Cuts at: 1563 3975 4348 1563 Size: 2412 373 3063 Fragments arranged by size: 3063 2412 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2426 3045 3911 5295 5563 1632 Size: 794 619 866 1384 268 1917 Fragments arranged by size: 1917 1384 866 794 619 268 AflII C'TTAA_G Cuts at: 820 1017 2249 820 Size: 197 1232 4419 Fragments arranged by size: 4419 1232 197 AflIII A'CryG_T Cuts at: 1203 3090 3092 4747 5724 1203 Size: 1887 2 1655 977 1327 Fragments arranged by size: 1887 1655 1327 977 2 AgeI A'CCGG_T Cuts at: 4679 4679 Size: 5848 AhdI GACnn_n'nnGTC Cuts at: 4577 4577 Size: 5848 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5848 AlwNI CAG_nnn'CTG Cuts at: 3591 5163 3591 Size: 1572 4276 ApaLI G'TGCA_C Cuts at: 3905 5061 3905 Size: 1156 4692 ApoI r'AATT_y Cuts at: 1493 2217 2317 2446 2471 2670 3157 3383 3535 Size: 724 100 129 25 199 487 226 152 Cuts at: 3535 5644 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 724 487 226 199 152 129 100 25 AseI AT'TA_AT Cuts at: 160 1032 4402 160 Size: 872 3370 1606 Fragments arranged by size: 3370 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 AvaI C'yCGr_G Cuts at: 1870 1943 2048 2171 3173 1870 Size: 73 105 123 1002 4545 Fragments arranged by size: 4545 1002 123 105 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2361 2394 2361 Size: 33 5815 BamHI G'GATC_C Cuts at: 3178 3545 3178 Size: 367 5481 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2302 3169 4624 618 Size: 325 217 627 515 867 1455 1842 Fragments arranged by size: 1842 1455 867 627 515 325 217 BanII G_rGCy'C Cuts at: 1391 3167 1391 Size: 1776 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3068 928 Size: 971 1169 3708 Fragments arranged by size: 3708 1169 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4039 4073 1903 Size: 34 2102 34 3678 Fragments arranged by size: 3678 2102 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2170 3740 4950 2170 Size: 1570 1210 3068 Fragments arranged by size: 3068 1570 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5848 BfrBI ATG'CAT Cuts at: 2759 2759 Size: 5848 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2253 2311 4459 Size: 107 122 71 631 605 581 58 2148 Cuts at: 4459 136 Size: 1525 Fragments arranged by size: 2148 1525 631 605 581 122 107 71 58 BglII A'GATC_T Cuts at: 5843 5843 Size: 5848 BlpI GC'TnA_GC Cuts at: 3265 3265 Size: 5848 Bme1580I G_kGCm'C Cuts at: 1790 2031 3090 3909 5065 1790 Size: 241 1059 819 1156 2573 Fragments arranged by size: 2573 1156 1059 819 241 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5848 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3579 4537 442 Size: 416 53 83 399 24 2162 958 1753 Fragments arranged by size: 2162 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5848 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2251 2251 Size: 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4508 1239 Size: 3269 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2964 3973 4838 5136 2964 Size: 1009 865 298 3676 Fragments arranged by size: 3676 1009 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4511 882 Size: 617 3012 2219 Fragments arranged by size: 3012 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3093 3639 493 Size: 711 1889 546 2702 Fragments arranged by size: 2702 1889 711 546 BsaBI GATnn'nnATC Cuts at: 5842 5842 Size: 5848 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3655 4037 4672 275 Size: 53 83 186 936 2122 382 635 1451 Fragments arranged by size: 2122 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4281 4679 4953 5100 5511 4281 Size: 398 274 147 411 4618 Fragments arranged by size: 4618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5623 Fragments arranged by size: 5623 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 1186 Size: 850 4998 BseYI C'CCAG_C Cuts at: 2139 5051 5547 2139 Size: 2912 496 2440 Fragments arranged by size: 2912 2440 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2683 1163 Size: 573 947 4328 Fragments arranged by size: 4328 947 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2021 2097 3298 4059 5087 1540 Size: 275 186 20 76 1201 761 1028 2301 Fragments arranged by size: 2301 1201 1028 761 275 186 76 20 BsiHKAI G_wGCw'C Cuts at: 2793 3909 3994 5065 2793 Size: 1116 85 1071 3576 Fragments arranged by size: 3576 1116 1071 85 BsmI GAATG_Cn' Cuts at: 2855 3359 3452 2855 Size: 504 93 5251 Fragments arranged by size: 5251 504 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2793 3090 3167 3909 3994 5065 Size: 399 241 762 297 77 742 85 1071 Cuts at: 5065 1391 Size: 2174 Fragments arranged by size: 2174 1071 762 742 399 297 241 85 77 BspEI T'CCGG_A Cuts at: 5511 5511 Size: 5848 BspHI T'CATG_A Cuts at: 3025 3737 5467 3025 Size: 712 1730 3406 Fragments arranged by size: 3406 1730 712 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3203 844 Size: 2359 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3735 1394 Size: 429 1912 3507 Fragments arranged by size: 3507 1912 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4343 4517 65 Size: 1183 3095 174 1396 Fragments arranged by size: 3095 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2092 4492 4679 1700 Size: 110 282 2400 187 2869 Fragments arranged by size: 2869 2400 282 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5848 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5848 BssSI C'ACGA_G Cuts at: 3908 4920 3908 Size: 1012 4836 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5848 BstBI TT'CG_AA Cuts at: 4652 4652 Size: 5848 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5848 BstYI r'GATC_y Cuts at: 1972 3178 3545 3930 3947 5399 5485 5497 5843 Size: 1206 367 385 17 1452 86 12 346 Cuts at: 5843 1972 Size: 1977 Fragments arranged by size: 1977 1452 1206 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2031 3132 513 Size: 552 966 1101 3229 Fragments arranged by size: 3229 1101 966 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5109 Fragments arranged by size: 5109 683 56 BtsI GCAGTG_nn' Cuts at: 3364 4158 4178 3364 Size: 794 20 5034 Fragments arranged by size: 5034 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5848 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5813 DraI TTT'AAA Cuts at: 2451 3152 2451 Size: 701 5147 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4855 809 Size: 507 3539 1802 Fragments arranged by size: 3539 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2018 2094 3295 Size: 54 1425 50 275 18 188 76 1201 Cuts at: 3295 4184 5813 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1201 889 275 188 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2018 2094 3295 1537 Size: 275 206 76 1201 4090 Fragments arranged by size: 4090 1201 275 206 76 EarI CTCTTCn'nnn_ Cuts at: 1353 2271 2401 3778 1353 Size: 918 130 1377 3423 Fragments arranged by size: 3423 1377 918 130 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4435 4809 4955 387 Size: 1064 138 2846 374 146 1280 Fragments arranged by size: 2846 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2426 3045 3911 4508 5295 5563 1239 Size: 393 794 619 866 597 787 268 1524 Fragments arranged by size: 1524 866 794 787 619 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2878 2878 Size: 5848 EcoRI G'AATT_C Cuts at: 3157 3535 3157 Size: 378 5470 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2430 2430 Size: 5848 FspI TGC'GCA Cuts at: 4354 4354 Size: 5848 HaeII r_GCGC'y Cuts at: 1678 2039 4995 1678 Size: 361 2956 2531 Fragments arranged by size: 2956 2531 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4489 4521 4563 4595 1373 Size: 32 135 32 2917 32 42 32 2626 Fragments arranged by size: 2917 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3105 3192 3438 669 Size: 988 281 1167 87 246 3079 Fragments arranged by size: 3079 1167 988 281 246 87 HindIII A'AGCT_T Cuts at: 748 2206 748 Size: 1458 4390 HpaI GTT'AAC Cuts at: 3438 3438 Size: 5848 KpnI G_GTAC'C Cuts at: 3173 3173 Size: 5848 MfeI C'AATT_G Cuts at: 3447 3447 Size: 5848 MluI A'CGCG_T Cuts at: 5724 5724 Size: 5848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2942 4962 5146 1305 Size: 1637 2020 184 2007 Fragments arranged by size: 2020 2007 1637 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4026 Fragments arranged by size: 4026 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2848 3024 3806 4165 4324 518 Size: 648 1682 176 782 359 159 2042 Fragments arranged by size: 2042 1682 782 648 359 176 159 MspA1I CmG'CkG Cuts at: 1606 2033 3264 3941 5089 5334 1606 Size: 427 1231 677 1148 245 2120 Fragments arranged by size: 2120 1231 1148 677 427 245 NaeI GCC'GGC Cuts at: 2094 2094 Size: 5848 NcoI C'CATG_G Cuts at: 513 1065 3132 513 Size: 552 2067 3229 Fragments arranged by size: 3229 2067 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5848 NgoMIV G'CCGG_C Cuts at: 2092 2092 Size: 5848 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5848 NotI GC'GGCC_GC Cuts at: 3295 3295 Size: 5848 NsiI A_TGCA'T Cuts at: 2761 2761 Size: 5848 NspI r_CATG'y Cuts at: 1132 1229 2763 3206 4751 1132 Size: 97 1534 443 1545 2229 Fragments arranged by size: 2229 1545 1534 443 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5848 PciI A'CATG_T Cuts at: 4747 4747 Size: 5848 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5848 PfoI T'CCnGG_A Cuts at: 2500 3005 2500 Size: 505 5343 PmeI GTTT'AAAC Cuts at: 3152 3152 Size: 5848 PmlI CAC'GTG Cuts at: 3093 3093 Size: 5848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3917 3949 5430 5462 3917 Size: 32 1481 32 4303 Fragments arranged by size: 4303 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2878 2878 Size: 5848 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5848 PsiI TTA'TAA Cuts at: 3418 3418 Size: 5848 PstI C_TGCA'G Cuts at: 830 3200 830 Size: 2370 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5848 SacII CC_GC'GG Cuts at: 2034 2034 Size: 5848 SalI G'TCGA_C Cuts at: 1936 3190 1936 Size: 1254 4594 SbfI CC_TGCA'GG Cuts at: 3200 3200 Size: 5848 ScaI AGT'ACT Cuts at: 4096 5808 4096 Size: 1712 4136 SexAI A'CCwGG_T Cuts at: 2533 2533 Size: 5848 SfcI C'TryA_G Cuts at: 826 1045 2922 3196 4331 5012 5203 826 Size: 219 1877 274 1135 681 191 1471 Fragments arranged by size: 1877 1471 1135 681 274 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5848 SmaI CCC'GGG Cuts at: 1872 3175 1872 Size: 1303 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2249 2979 3952 4853 5115 820 Size: 197 926 306 730 973 901 262 1553 Fragments arranged by size: 1553 973 926 901 730 306 262 197 SnaBI TAC'GTA Cuts at: 493 3639 493 Size: 3146 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5848 SphI G_CATG'C Cuts at: 1132 3206 1132 Size: 2074 3774 SspI AAT'ATT Cuts at: 5 52 3772 5 Size: 47 3720 2081 Fragments arranged by size: 3720 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5848 StyI C'CwwG_G Cuts at: 513 1065 2874 3132 3287 513 Size: 552 1809 258 155 3074 Fragments arranged by size: 3074 1809 552 258 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3875 3892 4045 4230 4675 325 Size: 1255 2295 17 153 185 445 1498 Fragments arranged by size: 2295 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2804 2993 4094 Size: 274 80 33 51 1443 827 189 1101 Cuts at: 4094 5806 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1101 827 274 189 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2372 2404 5808 5840 2372 Size: 32 3404 32 2380 Fragments arranged by size: 3404 2380 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5848 XbaI T'CTAG_A Cuts at: 3184 3184 Size: 5848 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5848 XmaI C'CCGG_G Cuts at: 1870 3173 1870 Size: 1303 4545 XmnI GAAnn'nnTTC Cuts at: 3058 3977 3058 Size: 919 4929 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3656 276 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI