(Circular) (Six-base) MAPSORT of: pFN21AB5748.seq Check: 7695 from: 1 to: 6943 pFN21AB5748 6943 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2903 2903 Size: 6943 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4753 278 Size: 53 83 186 936 3217 2468 Fragments arranged by size: 3217 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2352 2358 4286 1937 Size: 415 6 1928 4594 Fragments arranged by size: 4594 1928 415 6 Acc65I G'GTAC_C Cuts at: 2814 3777 4264 2814 Size: 963 487 5493 Fragments arranged by size: 5493 963 487 AclI AA'CG_TT Cuts at: 1563 5070 5443 1563 Size: 3507 373 3063 Fragments arranged by size: 3507 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3022 3132 3240 3950 5006 6390 6658 1632 Size: 1390 110 108 710 1056 1384 268 1917 Fragments arranged by size: 1917 1390 1384 1056 710 268 110 108 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6746 AflIII A'CryG_T Cuts at: 1203 5842 6819 1203 Size: 4639 977 1327 Fragments arranged by size: 4639 1327 977 AgeI A'CCGG_T Cuts at: 5774 5774 Size: 6943 AhdI GACnn_n'nnGTC Cuts at: 2500 5672 2500 Size: 3172 3771 AleI CACnn'nnGTG Cuts at: 1166 3602 1166 Size: 2436 4507 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6943 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3836 3868 3836 Size: 32 6911 AlwNI CAG_nnn'CTG Cuts at: 3872 4686 6258 3872 Size: 814 1572 4557 Fragments arranged by size: 4557 1572 814 ApaLI G'TGCA_C Cuts at: 2237 2516 5000 6156 2237 Size: 279 2484 1156 3024 Fragments arranged by size: 3024 2484 1156 279 ApoI r'AATT_y Cuts at: 1493 3440 4252 4478 4630 6739 1493 Size: 1947 812 226 152 2109 1697 Fragments arranged by size: 2109 1947 1697 812 226 152 AseI AT'TA_AT Cuts at: 160 1032 3120 5497 160 Size: 872 2088 2377 1606 Fragments arranged by size: 2377 2088 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6943 AvaI C'yCGr_G Cuts at: 1870 1943 4065 4268 1870 Size: 73 2122 203 4545 Fragments arranged by size: 4545 2122 203 73 BamHI G'GATC_C Cuts at: 4273 4640 4273 Size: 367 6576 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2814 3777 4264 5719 618 Size: 325 217 627 1027 963 487 1455 1842 Fragments arranged by size: 1842 1455 1027 963 627 487 325 217 BanII G_rGCy'C Cuts at: 1391 2426 4262 1391 Size: 1035 1836 4072 Fragments arranged by size: 4072 1836 1035 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5972 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4095 4129 5134 5168 1903 Size: 34 50 34 2074 34 1005 34 3678 Fragments arranged by size: 3678 2074 1005 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2473 3818 4835 6045 2473 Size: 1345 1017 1210 3371 Fragments arranged by size: 3371 1345 1210 1017 BclI T'GATC_A Cuts at: 1548 1548 Size: 6943 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5554 136 Size: 107 122 71 631 605 337 3545 1525 Fragments arranged by size: 3545 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6938 6938 Size: 6943 BlpI GC'TnA_GC Cuts at: 3083 3689 4360 3083 Size: 606 671 5666 Fragments arranged by size: 5666 671 606 Bme1580I G_kGCm'C Cuts at: 1790 2241 2520 3538 5004 6160 1790 Size: 451 279 1018 1466 1156 2573 Fragments arranged by size: 2573 1466 1156 1018 451 279 BmgBI CAC'GTC Cuts at: 1318 2623 2860 1318 Size: 1305 237 5401 Fragments arranged by size: 5401 1305 237 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2145 3520 4674 Size: 416 53 83 399 24 728 1375 1154 Cuts at: 4674 5632 442 Size: 958 1753 Fragments arranged by size: 1753 1375 1154 958 728 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6943 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2257 2410 3068 3372 5603 1239 Size: 1018 153 658 304 2231 2579 Fragments arranged by size: 2579 2231 1018 658 304 153 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5068 5933 6231 5068 Size: 865 298 5780 Fragments arranged by size: 5780 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2487 3481 4207 5606 882 Size: 617 988 994 726 1399 2219 Fragments arranged by size: 2219 1399 994 988 726 617 BsaAI yAC'GTr Cuts at: 493 1204 2356 3323 4734 493 Size: 711 1152 967 1411 2702 Fragments arranged by size: 2702 1411 1152 967 711 BsaBI GATnn'nnATC Cuts at: 3829 6937 3829 Size: 3108 3835 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3681 4750 5132 5767 Size: 53 83 186 936 2148 1069 382 635 Cuts at: 5767 275 Size: 1451 Fragments arranged by size: 2148 1451 1069 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2155 5376 5774 6048 6195 6606 2155 Size: 3221 398 274 147 411 2492 Fragments arranged by size: 3221 2492 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2735 2758 2765 2788 3836 Size: 30 165 30 1332 23 7 23 1048 Cuts at: 3836 3866 3890 3920 1178 Size: 30 24 30 4201 Fragments arranged by size: 4201 1332 1048 165 30 30 30 30 24 23 23 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2113 2766 2876 1186 Size: 927 653 110 5253 Fragments arranged by size: 5253 927 653 110 BseYI C'CCAG_C Cuts at: 2447 3486 4101 6146 6642 2447 Size: 1039 615 2045 496 2748 Fragments arranged by size: 2748 2045 1039 615 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2558 2972 1163 Size: 573 822 414 5134 Fragments arranged by size: 5134 822 573 414 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4393 5154 6182 1540 Size: 275 186 2392 761 1028 2301 Fragments arranged by size: 2392 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2241 2520 2886 3690 5004 5089 6160 2241 Size: 279 366 804 1314 85 1071 3024 Fragments arranged by size: 3024 1314 1071 804 366 279 85 BsmI GAATG_Cn' Cuts at: 2851 4454 4547 2851 Size: 1603 93 5247 Fragments arranged by size: 5247 1603 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2241 2426 2520 2886 3538 3690 4262 Size: 399 451 185 94 366 652 152 572 Cuts at: 4262 5004 5089 6160 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 652 572 451 399 366 185 152 94 85 BspEI T'CCGG_A Cuts at: 2155 6606 2155 Size: 4451 2492 BspHI T'CATG_A Cuts at: 3972 4832 6562 3972 Size: 860 1730 4353 Fragments arranged by size: 4353 1730 860 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2903 3924 4298 844 Size: 2059 1021 374 3489 Fragments arranged by size: 3489 2059 1021 374 BsrBI CCG'CTC Cuts at: 1394 1823 3817 4830 1394 Size: 429 1994 1013 3507 Fragments arranged by size: 3507 1994 1013 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3094 5438 5612 65 Size: 1183 1846 2344 174 1396 Fragments arranged by size: 2344 1846 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5587 5774 1700 Size: 110 3777 187 2869 Fragments arranged by size: 3777 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6943 BssHII G'CGCG_C Cuts at: 1925 3785 1925 Size: 1860 5083 BssSI C'ACGA_G Cuts at: 3015 3506 5003 6015 3015 Size: 491 1497 1012 3943 Fragments arranged by size: 3943 1497 1012 491 BstAPI GCAn_nnn'nTGC Cuts at: 794 2295 794 Size: 1501 5442 BstBI TT'CG_AA Cuts at: 5747 5747 Size: 6943 BstEII G'GTnAC_C Cuts at: 1184 3463 1184 Size: 2279 4664 BstYI r'GATC_y Cuts at: 1972 2528 4273 4640 5025 5042 6494 6580 6592 Size: 556 1745 367 385 17 1452 86 12 Cuts at: 6592 6938 1972 Size: 346 1977 Fragments arranged by size: 1977 1745 1452 556 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2359 2359 Size: 6943 BtgI C'CryG_G Cuts at: 513 1065 3855 513 Size: 552 2790 3601 Fragments arranged by size: 3601 2790 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2660 3162 487 Size: 683 56 1434 502 4268 Fragments arranged by size: 4268 1434 683 502 56 BtsI GCAGTG_nn' Cuts at: 3444 3597 4459 5253 5273 3444 Size: 153 862 794 20 5114 Fragments arranged by size: 5114 862 794 153 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6943 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3218 3253 3708 3743 529 Size: 35 2654 35 455 35 3729 Fragments arranged by size: 3729 2654 455 35 35 35 DraI TTT'AAA Cuts at: 3426 4192 4247 3426 Size: 766 55 6122 Fragments arranged by size: 6122 766 55 DraIII CAC_nnn'GTG Cuts at: 3747 3747 Size: 6943 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5950 809 Size: 507 4634 1802 Fragments arranged by size: 4634 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2412 2638 2652 Size: 54 1425 50 275 18 582 226 14 Cuts at: 2652 3951 4390 5279 6908 8 Size: 1299 439 889 1629 43 Fragments arranged by size: 1629 1425 1299 889 582 439 275 226 54 50 43 18 14 EagI C'GGCC_G Cuts at: 1537 1812 4390 1537 Size: 275 2578 4090 Fragments arranged by size: 4090 2578 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2804 4873 1353 Size: 1451 2069 3423 Fragments arranged by size: 3423 2069 1451 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5530 5904 6050 387 Size: 1064 138 3941 374 146 1280 Fragments arranged by size: 3941 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2257 2410 3022 3068 3132 3240 3372 Size: 393 625 153 612 46 64 108 132 Cuts at: 3372 3950 5006 5603 6390 6658 1239 Size: 578 1056 597 787 268 1524 Fragments arranged by size: 1524 1056 787 625 612 597 578 393 268 153 132 108 64 46 EcoNI CCTnn'n_nnAGG Cuts at: 3698 3698 Size: 6943 EcoO109I rG'GnC_Cy Cuts at: 3033 3033 Size: 6943 EcoRI G'AATT_C Cuts at: 4252 4630 4252 Size: 378 6565 FspI TGC'GCA Cuts at: 5449 5449 Size: 6943 HaeII r_GCGC'y Cuts at: 1678 6090 1678 Size: 4412 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2255 2287 2486 2518 4147 Size: 32 135 32 683 32 199 32 1629 Cuts at: 4147 4179 4187 4219 5584 5616 5658 5690 1373 Size: 32 8 32 1365 32 42 32 2626 Fragments arranged by size: 2626 1629 1365 683 199 135 42 32 32 32 32 32 32 32 32 8 HincII GTy'rAC Cuts at: 669 1657 1938 3864 4287 4533 669 Size: 988 281 1926 423 246 3079 Fragments arranged by size: 3079 1926 988 423 281 246 HindIII A'AGCT_T Cuts at: 748 3500 748 Size: 2752 4191 HpaI GTT'AAC Cuts at: 4533 4533 Size: 6943 KpnI G_GTAC'C Cuts at: 2818 3781 4268 2818 Size: 963 487 5493 Fragments arranged by size: 5493 963 487 MfeI C'AATT_G Cuts at: 2213 3947 3955 4542 2213 Size: 1734 8 587 4614 Fragments arranged by size: 4614 1734 587 8 MluI A'CGCG_T Cuts at: 6819 6819 Size: 6943 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2707 6057 6241 1305 Size: 1402 3350 184 2007 Fragments arranged by size: 3350 2007 1402 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2654 3953 10 Size: 54 1425 343 822 1299 3000 Fragments arranged by size: 3000 1425 1299 822 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3602 3835 3971 4901 5260 5419 518 Size: 648 2436 233 136 930 359 159 2042 Fragments arranged by size: 2436 2042 930 648 359 233 159 136 MspA1I CmG'CkG Cuts at: 1606 2042 2127 3588 4134 4153 4359 5036 6184 Size: 436 85 1461 546 19 206 677 1148 Cuts at: 6184 6429 1606 Size: 245 2120 Fragments arranged by size: 2120 1461 1148 677 546 436 245 206 85 19 NcoI C'CATG_G Cuts at: 513 1065 3855 513 Size: 552 2790 3601 Fragments arranged by size: 3601 2790 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6943 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6943 NotI GC'GGCC_GC Cuts at: 4390 4390 Size: 6943 NspI r_CATG'y Cuts at: 1132 1229 2794 4150 4301 5846 1132 Size: 97 1565 1356 151 1545 2229 Fragments arranged by size: 2229 1565 1545 1356 151 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6943 PciI A'CATG_T Cuts at: 5842 5842 Size: 6943 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6943 PfoI T'CCnGG_A Cuts at: 2178 3045 3349 2178 Size: 867 304 5772 Fragments arranged by size: 5772 867 304 PmeI GTTT'AAAC Cuts at: 4247 4247 Size: 6943 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3836 3868 5012 5044 6525 6557 3836 Size: 32 1144 32 1481 32 4222 Fragments arranged by size: 4222 1481 1144 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3033 3033 Size: 6943 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6943 PsiI TTA'TAA Cuts at: 4513 4513 Size: 6943 PstI C_TGCA'G Cuts at: 830 3451 3517 4295 830 Size: 2621 66 778 3478 Fragments arranged by size: 3478 2621 778 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6943 PvuII CAG'CTG Cuts at: 2042 4134 2042 Size: 2092 4851 SalI G'TCGA_C Cuts at: 1936 4285 1936 Size: 2349 4594 SbfI CC_TGCA'GG Cuts at: 4295 4295 Size: 6943 ScaI AGT'ACT Cuts at: 2671 3530 5191 6903 2671 Size: 859 1661 1712 2711 Fragments arranged by size: 2711 1712 1661 859 SexAI A'CCwGG_T Cuts at: 4197 4197 Size: 6943 SfcI C'TryA_G Cuts at: 826 1045 3447 3513 4291 5426 6107 6298 826 Size: 219 2402 66 778 1135 681 191 1471 Fragments arranged by size: 2402 1471 1135 778 681 219 191 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6943 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6943 SmaI CCC'GGG Cuts at: 1872 4270 1872 Size: 2398 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5047 5948 6210 820 Size: 197 926 3104 901 262 1553 Fragments arranged by size: 3104 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2356 4734 493 Size: 1863 2378 2702 Fragments arranged by size: 2702 2378 1863 SpeI A'CTAG_T Cuts at: 152 152 Size: 6943 SphI G_CATG'C Cuts at: 1132 4301 1132 Size: 3169 3774 SspI AAT'ATT Cuts at: 5 52 3306 3890 4867 5 Size: 47 3254 584 977 2081 Fragments arranged by size: 3254 2081 977 584 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6943 StyI C'CwwG_G Cuts at: 513 1065 2204 3855 4382 513 Size: 552 1139 1651 527 3074 Fragments arranged by size: 3074 1651 1139 552 527 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4970 4987 5140 5325 5770 325 Size: 1255 3390 17 153 185 445 1498 Fragments arranged by size: 3390 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2669 2787 3528 Size: 274 80 33 51 1443 692 118 741 Cuts at: 3528 5189 6901 96 Size: 1661 1712 138 Fragments arranged by size: 1712 1661 1443 741 692 274 138 118 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2758 2790 6903 6935 2758 Size: 32 4113 32 2766 Fragments arranged by size: 4113 2766 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4181 4199 1316 Size: 2865 18 4060 Fragments arranged by size: 4060 2865 18 XbaI T'CTAG_A Cuts at: 4279 4279 Size: 6943 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3004 3004 Size: 6943 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6943 XmaI C'CCGG_G Cuts at: 1870 4268 1870 Size: 2398 4545 XmnI GAAnn'nnTTC Cuts at: 5072 5072 Size: 6943 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4751 276 Size: 53 83 186 936 3217 2468 Fragments arranged by size: 3217 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI