################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglI BlnI BsaAI BsaBI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AcyI 1937 GACGCC G[GA]^CG[CT]C AflII 889 CTTAAG C^TTAAG AgeI 2216 ACCGGT A^CCGGT Asp700I 1561 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 433 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 689 CTCGGG C^[CT]CG[GA]G BfrI 889 CTTAAG C^TTAAG BsaHI 1937 GACGCC G[GA]^CG[CT]C BsaI 1509 GAGACC GGTCTC(1/5) BsaWI 2216 ACCGGT [AT]^CCGG[AT] BsmAI 1509 GAGAC GTCTC(1/5) BsoBI 689 CTCGGG C^[CT]CG[GA]G BspLU11I 516 ACATGT A^CATGT BspMI 1473 ACCTGC ACCTGC(4/8) BsrDI 501 CATTGC GCAATG(2/0) CfoI 2016 GCGC GCG^C DsaI 1203 CCATGG C^C[GA][CT]GG HhaI 2016 GCGC GCG^C Hin1I 1937 GACGCC G[GA]^CG[CT]C HinP1I 2016 GCGC G^CGC NcoI 1203 CCATGG C^CATGG NspI 516 ACATGT [GA]CATG^[CT] PflFI 433 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2216 ACCGGT A^CCGGT PleI 326 GAGTC GAGTC(4/5) SacI 1303 GAGCTC GAGCT^C ScaI 1383 AGTACT AGT^ACT StuI 626 AGGCCT AGG^CCT Tth111I 433 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 938 CCAAATATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1561 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AflIII 516 ACATGT A^C[GA][CT]GT 2275 ACACGT AocI 1645 CCTAAGG CC^T[ACGT]AGG 1960 CCTGAGG AspHI 520 GTGCTC G[AT]GC[AT]^C 1303 GAGCTC BalI 299 TGGCCA TGG^CCA 935 TGGCCA BamHI 380 GGATCC G^GATCC 2110 GGATCC BglII 264 AGATCT A^GATCT 1297 AGATCT BlpI 1305 GCTCAGC GC^T[ACGT]AGC 1318 GCTGAGC Bpu1102I 1305 GCTCAGC GC^T[ACGT]AGC 1318 GCTGAGC BsgI 1137 CTGCAC GTGCAG(16/14) 1093 GTGCAG BsiHKAI 520 GTGCTC G[AT]GC[AT]^C 1303 GAGCTC BsmI 1101 GAATGC GAATGC(1/-1) 397 GCATTC BstXI 866 CCAGAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 897 CCAAAACAGTGG Bsu36I 1645 CCTAAGG CC^T[ACGT]AGG 1960 CCTGAGG CelII 1305 GCTCAGC GC^T[ACGT]AGC 1318 GCTGAGC DrdI 433 GACCCTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2236 GACCTGTCTGTC EaeI 299 TGGCCA [CT]^GGCC[GA] 935 TGGCCA Eco81I 1645 CCTAAGG CC^T[ACGT]AGG 1960 CCTGAGG EcoNI 549 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 648 CCTAGAAGAGG HgaI 1527 GACGC GACGC(5/10) 1937 GACGC HindIII 133 AAGCTT A^AGCTT 1790 AAGCTT HphI 2165 TCACC GGTGA(8/7) 2270 TCACC MluNI 299 TGGCCA TGG^CCA 935 TGGCCA MscI 299 TGGCCA TGG^CCA 935 TGGCCA PstI 697 CTGCAG CTGCA^G 830 CTGCAG SapI 1534 GAAGAGC GCTCTTC(1/4) 1543 GAAGAGC TaqI 723 TCGA T^CGA 1676 TCGA TthHB8I 723 TCGA T^CGA 1676 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII