(Linear) (Six-base) MAPSORT of: KIEE1533.seq Check: 1504 from: 1 to: 2313 KIEE1533.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 254 466 664 2313 Size: 226 28 212 198 1649 Fragments arranged by size: 1649 226 212 198 28 AflII C'TTAA_G Cuts at: 0 889 2313 Size: 889 1424 AflIII A'CryG_T Cuts at: 0 516 2275 2313 Size: 516 1759 38 Fragments arranged by size: 1759 516 38 AgeI A'CCGG_T Cuts at: 0 2216 2313 Size: 2216 97 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1118 2313 Size: 1118 1195 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1055 1087 2313 Size: 1055 32 1226 Fragments arranged by size: 1226 1055 32 AlwNI CAG_nnn'CTG Cuts at: 0 530 1136 1530 2313 Size: 530 606 394 783 Fragments arranged by size: 783 606 530 394 ApoI r'AATT_y Cuts at: 0 560 1253 1267 1986 2313 Size: 560 693 14 719 327 Fragments arranged by size: 719 693 560 327 14 AvaI C'yCGr_G Cuts at: 0 689 2313 Size: 689 1624 BamHI G'GATC_C Cuts at: 0 380 2110 2313 Size: 380 1730 203 Fragments arranged by size: 1730 380 203 BanII G_rGCy'C Cuts at: 0 690 696 1307 1496 2313 Size: 690 6 611 189 817 Fragments arranged by size: 817 690 611 189 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 346 2210 2213 2313 Size: 346 1864 3 100 Fragments arranged by size: 1864 346 100 3 BglII A'GATC_T Cuts at: 0 264 1297 2313 Size: 264 1033 1016 Fragments arranged by size: 1033 1016 264 BlpI GC'TnA_GC Cuts at: 0 1306 1319 2313 Size: 1306 13 994 Fragments arranged by size: 1306 994 13 BmgBI CAC'GTC Cuts at: 0 1869 2208 2278 2313 Size: 1869 339 70 35 Fragments arranged by size: 1869 339 70 35 BmrI ACTGGGnnnn_n' Cuts at: 0 1916 2313 Size: 1916 397 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 502 571 970 994 2313 Size: 502 69 399 24 1319 Fragments arranged by size: 1319 502 399 69 24 Bpu10I CC'TnA_GC Cuts at: 0 1129 1178 2313 Size: 1129 49 1135 Fragments arranged by size: 1135 1129 49 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 102 292 1216 2211 2313 Size: 102 190 924 995 102 Fragments arranged by size: 995 924 190 102 102 BsaI GGTCTCn'nnnn_ Cuts at: 0 1503 2313 Size: 1503 810 BsaHI Gr'CG_yC Cuts at: 0 1938 2313 Size: 1938 375 BsaWI w'CCGG_w Cuts at: 0 2216 2313 Size: 2216 97 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 992 1022 1354 1384 1483 1513 1673 1689 Size: 992 30 332 30 99 30 160 16 Cuts at: 1689 1703 1719 2313 Size: 14 16 594 Fragments arranged by size: 992 594 332 160 99 30 30 30 16 16 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 505 562 1051 1504 1756 1774 2313 Size: 505 57 489 453 252 18 539 Fragments arranged by size: 539 505 489 453 252 57 18 BseYI C'CCAG_C Cuts at: 0 1548 1798 1889 2313 Size: 1548 250 91 424 Fragments arranged by size: 1548 424 250 91 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1114 1122 2313 Size: 1114 8 1191 Fragments arranged by size: 1191 1114 8 BsiHKAI G_wGCw'C Cuts at: 0 524 1307 2313 Size: 524 783 1006 Fragments arranged by size: 1006 783 524 BsmI GAATG_Cn' Cuts at: 0 397 1107 2313 Size: 397 710 1206 Fragments arranged by size: 1206 710 397 Bsp1286I G_dGCh'C Cuts at: 0 524 690 696 1307 1496 2313 Size: 524 166 6 611 189 817 Fragments arranged by size: 817 611 524 189 166 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1482 2313 Size: 1482 831 BsrDI GCAATG_nn' Cuts at: 0 500 2313 Size: 500 1813 BsrFI r'CCGG_y Cuts at: 0 11 452 1940 2216 2313 Size: 11 441 1488 276 97 Fragments arranged by size: 1488 441 276 97 11 BstXI CCAn_nnnn'nTGG Cuts at: 0 873 904 2313 Size: 873 31 1409 Fragments arranged by size: 1409 873 31 BstYI r'GATC_y Cuts at: 0 264 380 1297 2110 2313 Size: 264 116 917 813 203 Fragments arranged by size: 917 813 264 203 116 Bsu36I CC'TnA_GG Cuts at: 0 1646 1961 2313 Size: 1646 315 352 Fragments arranged by size: 1646 352 315 BtgI C'CryG_G Cuts at: 0 1203 2313 Size: 1203 1110 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1850 1885 2313 Size: 1850 35 428 Fragments arranged by size: 1850 428 35 DrdI GACnn_nn'nnGTC Cuts at: 0 439 2242 2313 Size: 439 1803 71 Fragments arranged by size: 1803 439 71 EaeI y'GGCC_r Cuts at: 0 299 935 2313 Size: 299 636 1378 Fragments arranged by size: 1378 636 299 EarI CTCTTCn'nnn_ Cuts at: 0 362 647 720 1028 1481 1529 1538 1751 Size: 362 285 73 308 453 48 9 213 Cuts at: 1751 1828 2313 Size: 77 485 Fragments arranged by size: 485 453 362 308 285 213 77 73 48 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 189 2313 Size: 189 2124 EcoICRI GAG'CTC Cuts at: 0 1305 2313 Size: 1305 1008 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 254 466 502 571 664 970 994 Size: 226 28 212 36 69 93 306 24 Cuts at: 994 2313 Size: 1319 Fragments arranged by size: 1319 306 226 212 93 69 36 28 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 553 652 2313 Size: 553 99 1661 Fragments arranged by size: 1661 553 99 EcoO109I rG'GnC_Cy Cuts at: 0 432 1173 1619 1966 2313 Size: 432 741 446 347 347 Fragments arranged by size: 741 446 432 347 347 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1084 2313 Size: 1084 1229 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 111 143 1289 1321 1354 1386 2313 Size: 111 32 1146 32 33 32 927 Fragments arranged by size: 1146 927 111 33 32 32 32 HindIII A'AGCT_T Cuts at: 0 133 1790 2313 Size: 133 1657 523 Fragments arranged by size: 1657 523 133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2213 2313 Size: 2213 100 MscI TGG'CCA Cuts at: 0 301 937 2313 Size: 301 636 1376 Fragments arranged by size: 1376 636 301 MspA1I CmG'CkG Cuts at: 0 468 810 829 2032 2292 2313 Size: 468 342 19 1203 260 21 Fragments arranged by size: 1203 468 342 260 21 19 NcoI C'CATG_G Cuts at: 0 1203 2313 Size: 1203 1110 NspI r_CATG'y Cuts at: 0 520 2313 Size: 520 1793 PciI A'CATG_T Cuts at: 0 516 2313 Size: 516 1797 PfoI T'CCnGG_A Cuts at: 0 1417 2313 Size: 1417 896 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 506 538 2313 Size: 506 32 1775 Fragments arranged by size: 1775 506 32 PpuMI rG'GwC_Cy Cuts at: 0 432 1173 1619 1966 2313 Size: 432 741 446 347 347 Fragments arranged by size: 741 446 432 347 347 PstI C_TGCA'G Cuts at: 0 701 834 2313 Size: 701 133 1479 Fragments arranged by size: 1479 701 133 PvuII CAG'CTG Cuts at: 0 468 810 829 2032 2292 2313 Size: 468 342 19 1203 260 21 Fragments arranged by size: 1203 468 342 260 21 19 SacI G_AGCT'C Cuts at: 0 1307 2313 Size: 1307 1006 SanDI GG'GwC_CC Cuts at: 0 432 2313 Size: 432 1881 SapI GCTCTTCn'nnn_ Cuts at: 0 1529 1538 2313 Size: 1529 9 775 Fragments arranged by size: 1529 775 9 ScaI AGT'ACT Cuts at: 0 1385 2313 Size: 1385 928 SfcI C'TryA_G Cuts at: 0 697 830 1504 1681 2306 2313 Size: 697 133 674 177 625 7 Fragments arranged by size: 697 674 625 177 133 7 SmlI C'TyrA_G Cuts at: 0 81 271 889 1195 2226 2313 Size: 81 190 618 306 1031 87 Fragments arranged by size: 1031 618 306 190 87 81 StuI AGG'CCT Cuts at: 0 628 2313 Size: 628 1685 StyI C'CwwG_G Cuts at: 0 716 1203 2156 2313 Size: 716 487 953 157 Fragments arranged by size: 953 716 487 157 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2140 2313 Size: 2140 173 TatI w'GTAC_w Cuts at: 0 1383 1521 2313 Size: 1383 138 792 Fragments arranged by size: 1383 792 138 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 992 1024 1689 1721 2313 Size: 992 32 665 32 592 Fragments arranged by size: 992 665 592 32 32 Tth111I GACn'n_nGTC Cuts at: 0 436 2313 Size: 436 1877 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 945 2313 Size: 945 1368 XmnI GAAnn'nnTTC Cuts at: 0 1565 2313 Size: 1565 748 Enzymes that do cut: AcuI AflII AflIII AgeI AlfI AloI AlwNI ApoI AvaI BamHI BanII BbsI BglII BlpI BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BstXI BstYI Bsu36I BtgI CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HindIII MmeI MscI MspA1I NcoI NspI PciI PfoI PpiI PpuMI PstI PvuII SacI SanDI SapI ScaI SfcI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AhdI AleI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI Bme1580I BmtI BplI BsaAI BsaBI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI DraI DraIII EagI EcoRI EcoRV FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI ZraI