Restriction map for
pFN21AB6301
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaII AviII AvrII
BalI BamHI BanII BbrPI BbsI
BbvCI BbvI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BseAI BseRI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DpnII DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeI
MaeII MamI MboI MfeI MflI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI SexAI
SfcI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI TseI
Tth111I TthHB8I XbaI XcmI XhoI
XhoII
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# ONE CUT ENZYMES ( 28 enzymes) #
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AccI 232 GTCTAC GT^[AC][GT]AC
AciI 251 GCGG CCGC(-3/-1)
AcsI 704 GAATTT [GA]^AATT[CT]
ApoI 704 GAATTT [GA]^AATT[CT]
Asp700I 585 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 136 CCCGGG C^[CT]CG[GA]G
BglI 314 GCCCCTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 292 ACCGGA [AT]^CCGG[AT]
BsgI 221 CTGCAC GTGCAG(16/14)
BsoBI 136 CCCGGG C^[CT]CG[GA]G
BstF5I 61 GGATG GGATG(2/0)
BstXI 84 CCAGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Fnu4HI 251 GCGGC GC^[ACGT]GC
FokI 61 GGATG GGATG(9/13)
ItaI 251 GCGGC GC^[ACGT]GC
MaeIII 713 GTGAC ^GT[ACGT]AC
MspA1I 249 CAGCGG C[AC]G^C[GT]G
NspI 386 GCATGC [GA]CATG^[CT]
PflMI 268 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 603 AGTACT AGT^ACT
SmaI 136 CCCGGG CCC^GGG
SmlI 56 CTCAAG C^T[CT][GA]AG
SphI 386 GCATGC GCATG^C
Tsp45I 713 GTGAC ^GT[GC]AC
Van91I 268 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 136 CCCGGG C^CCGGG
XmaI 136 CCCGGG C^CCGGG
XmnI 585 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 181 GGCGCC G[GA]^CG[CT]C
322 GGCGCC
AfaI 604 GTAC GT^AC
660 GTAC
AluI 204 AGCT AG^CT
527 AGCT
AspHI 194 GAGCAC G[AT]GC[AT]^C
215 GAGCAC
BbeI 181 GGCGCC GGCGC^C
322 GGCGCC
BsaHI 181 GGCGCC G[GA]^CG[CT]C
322 GGCGCC
BsiHKAI 194 GAGCAC G[AT]GC[AT]^C
215 GAGCAC
BspMI 219 ACCTGC ACCTGC(4/8)
356 ACCTGC
DraII 127 GGGGCCT [GA]G^G[ACGT]CC[CT]
259 GGGGCCT
EcoO109I 127 GGGGCCT [GA]G^G[ACGT]CC[CT]
259 GGGGCCT
HaeII 181 GGCGCC [GA]GCGC^[CT]
322 GGCGCC
Hin1I 181 GGCGCC G[GA]^CG[CT]C
322 GGCGCC
HinfI 230 GAGTC G^A[ACGT]TC
506 GAGTC
KasI 181 GGCGCC G^GCGCC
322 GGCGCC
MboII 550 GAAGA GAAGA(8/7)
271 TCTTC
MseI 476 TTAA T^TAA
674 TTAA
NarI 181 GGCGCC GG^CGCC
322 GGCGCC
PleI 230 GAGTC GAGTC(4/5)
506 GAGTC
RsaI 604 GTAC GT^AC
660 GTAC
SfaNI 25 GATGC GCATC(5/9)
643 GATGC
SfoI 181 GGCGCC GGC^GCC
322 GGCGCC
Tru9I 476 TTAA T^TAA
674 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
BanI BcnI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cac8I CfoI Cfr13I EcoRII
HaeIII HapII HhaI HinP1I HpaII
HphI MnlI MspI MvaI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
Tsp509I TspRI