(Circular) (Six-base) MAPSORT of: pFN21AB6301.seq Check: 6347 from: 1 to: 5455 pFN21AB6301 5455 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2233 2233 Size: 5455 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3265 278 Size: 53 83 186 936 1729 2468 Fragments arranged by size: 2468 1729 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2238 2798 1937 Size: 301 560 4594 Fragments arranged by size: 4594 560 301 Acc65I G'GTAC_C Cuts at: 2776 2776 Size: 5455 AclI AA'CG_TT Cuts at: 1563 3582 3955 1563 Size: 2019 373 3063 Fragments arranged by size: 3063 2019 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3518 4902 5170 1632 Size: 1886 1384 268 1917 Fragments arranged by size: 1917 1886 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5258 AflIII A'CryG_T Cuts at: 1203 4354 5331 1203 Size: 3151 977 1327 Fragments arranged by size: 3151 1327 977 AgeI A'CCGG_T Cuts at: 4286 4286 Size: 5455 AhdI GACnn_n'nnGTC Cuts at: 4184 4184 Size: 5455 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5455 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5455 AlwNI CAG_nnn'CTG Cuts at: 3198 4770 3198 Size: 1572 3883 ApaLI G'TGCA_C Cuts at: 3512 4668 3512 Size: 1156 4299 ApoI r'AATT_y Cuts at: 1493 2709 2764 2990 3142 5251 1493 Size: 1216 55 226 152 2109 1697 Fragments arranged by size: 2109 1697 1216 226 152 55 AseI AT'TA_AT Cuts at: 160 1032 4009 160 Size: 872 2977 1606 Fragments arranged by size: 2977 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 AvaI C'yCGr_G Cuts at: 1870 1943 2141 2780 1870 Size: 73 198 639 4545 Fragments arranged by size: 4545 639 198 73 BamHI G'GATC_C Cuts at: 2785 3152 2785 Size: 367 5088 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2149 2176 2186 2327 2383 Size: 325 217 627 362 27 10 141 56 Cuts at: 2383 2776 4231 618 Size: 393 1455 1842 Fragments arranged by size: 1842 1455 627 393 362 325 217 141 56 27 10 BanII G_rGCy'C Cuts at: 1391 2774 1391 Size: 1383 4072 BbeI G_GCGC'C Cuts at: 2190 2331 2190 Size: 141 5314 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4484 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3646 3680 1903 Size: 34 1709 34 3678 Fragments arranged by size: 3678 1709 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3347 4557 3347 Size: 1210 4245 BclI T'GATC_A Cuts at: 1548 1548 Size: 5455 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2325 4066 136 Size: 107 122 71 631 605 653 1741 1525 Fragments arranged by size: 1741 1525 653 631 605 122 107 71 BglII A'GATC_T Cuts at: 5450 5450 Size: 5455 BlpI GC'TnA_GC Cuts at: 2872 2872 Size: 5455 Bme1580I G_kGCm'C Cuts at: 1790 2414 3516 4672 1790 Size: 624 1102 1156 2573 Fragments arranged by size: 2573 1156 1102 624 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5455 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2554 2714 3186 Size: 416 53 83 399 24 1137 160 472 Cuts at: 3186 4144 442 Size: 958 1753 Fragments arranged by size: 1753 1137 958 472 416 399 160 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5455 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2166 2217 2226 2253 4115 1239 Size: 927 51 9 27 1862 2579 Fragments arranged by size: 2579 1862 927 51 27 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2046 3580 4445 4743 2046 Size: 1534 865 298 2758 Fragments arranged by size: 2758 1534 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4118 882 Size: 617 2619 2219 Fragments arranged by size: 2619 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3246 493 Size: 711 2042 2702 Fragments arranged by size: 2702 2042 711 BsaBI GATnn'nnATC Cuts at: 5449 5449 Size: 5455 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2187 2328 3262 3644 Size: 53 83 186 936 654 141 934 382 Cuts at: 3644 4279 275 Size: 635 1451 Fragments arranged by size: 1451 936 934 654 635 382 186 141 83 53 BsaWI w'CCGG_w Cuts at: 2297 3888 4286 4560 4707 5118 2297 Size: 1591 398 274 147 411 2634 Fragments arranged by size: 2634 1591 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5230 Fragments arranged by size: 5230 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5455 BseYI C'CCAG_C Cuts at: 2096 2252 2336 4658 5154 2096 Size: 156 84 2322 496 2397 Fragments arranged by size: 2397 2322 496 156 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2211 1163 Size: 573 475 4407 Fragments arranged by size: 4407 573 475 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2905 3666 4694 1540 Size: 275 186 904 761 1028 2301 Fragments arranged by size: 2301 1028 904 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2203 2224 3516 3601 4672 2203 Size: 21 1292 85 1071 2986 Fragments arranged by size: 2986 1292 1071 85 21 BsmI GAATG_Cn' Cuts at: 2966 3059 2966 Size: 93 5362 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2203 2224 2414 2774 3516 3601 4672 Size: 399 413 21 190 360 742 85 1071 Cuts at: 4672 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 413 399 360 190 85 21 BspEI T'CCGG_A Cuts at: 5118 5118 Size: 5455 BspHI T'CATG_A Cuts at: 3344 5074 3344 Size: 1730 3725 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2233 2370 2810 844 Size: 1389 137 440 3489 Fragments arranged by size: 3489 1389 440 137 BsrBI CCG'CTC Cuts at: 1394 1823 3342 1394 Size: 429 1519 3507 Fragments arranged by size: 3507 1519 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3950 4124 65 Size: 1183 2702 174 1396 Fragments arranged by size: 2702 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4099 4286 1700 Size: 110 2289 187 2869 Fragments arranged by size: 2869 2289 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5455 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5455 BssSI C'ACGA_G Cuts at: 3515 4527 3515 Size: 1012 4443 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5455 BstBI TT'CG_AA Cuts at: 4259 4259 Size: 5455 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5455 BstXI CCAn_nnnn'nTGG Cuts at: 2096 2096 Size: 5455 BstYI r'GATC_y Cuts at: 1972 2785 3152 3537 3554 5006 5092 5104 5450 Size: 813 367 385 17 1452 86 12 346 Cuts at: 5450 1972 Size: 1977 Fragments arranged by size: 1977 1452 813 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3964 Fragments arranged by size: 3964 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4716 Fragments arranged by size: 4716 683 56 BtsI GCAGTG_nn' Cuts at: 2646 2971 3765 3785 2646 Size: 325 794 20 4316 Fragments arranged by size: 4316 794 325 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5455 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5420 DraI TTT'AAA Cuts at: 2759 2759 Size: 5455 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4462 809 Size: 507 3146 1802 Fragments arranged by size: 3146 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2902 3791 5420 Size: 54 1425 50 275 18 1072 889 1629 Cuts at: 5420 8 Size: 43 Fragments arranged by size: 1629 1425 1072 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2902 1537 Size: 275 1090 4090 Fragments arranged by size: 4090 1090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3385 1353 Size: 2032 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4042 4416 4562 387 Size: 1064 138 2453 374 146 1280 Fragments arranged by size: 2453 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2166 2217 2226 2253 3518 4115 4902 Size: 393 534 51 9 27 1265 597 787 Cuts at: 4902 5170 1239 Size: 268 1524 Fragments arranged by size: 1524 1265 787 597 534 393 268 51 27 9 EcoO109I rG'GnC_Cy Cuts at: 2133 2265 2133 Size: 132 5323 EcoRI G'AATT_C Cuts at: 2764 3142 2764 Size: 378 5077 FspI TGC'GCA Cuts at: 3961 3961 Size: 5455 HaeII r_GCGC'y Cuts at: 1678 2190 2331 4602 1678 Size: 512 141 2271 2531 Fragments arranged by size: 2531 2271 512 141 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 4096 4128 4170 Size: 32 135 32 449 32 2043 32 42 Cuts at: 4170 4202 1373 Size: 32 2626 Fragments arranged by size: 2626 2043 449 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2799 3045 669 Size: 988 281 861 246 3079 Fragments arranged by size: 3079 988 861 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5455 HpaI GTT'AAC Cuts at: 3045 3045 Size: 5455 KasI G'GCGC_C Cuts at: 2186 2327 2186 Size: 141 5314 KpnI G_GTAC'C Cuts at: 2780 2780 Size: 5455 MfeI C'AATT_G Cuts at: 3054 3054 Size: 5455 MluI A'CGCG_T Cuts at: 5331 5331 Size: 5455 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4569 4753 1305 Size: 3264 184 2007 Fragments arranged by size: 3264 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3633 Fragments arranged by size: 3633 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3413 3772 3931 518 Size: 648 2247 359 159 2042 Fragments arranged by size: 2247 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2256 2871 3548 4696 4941 1606 Size: 650 615 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 650 615 245 NarI GG'CG_CC Cuts at: 2187 2328 2187 Size: 141 5314 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3964 Fragments arranged by size: 3964 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5455 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5455 NotI GC'GGCC_GC Cuts at: 2902 2902 Size: 5455 NspI r_CATG'y Cuts at: 1132 1229 2395 2813 4358 1132 Size: 97 1166 418 1545 2229 Fragments arranged by size: 2229 1545 1166 418 97 PasI CC'CwG_GG Cuts at: 1348 2114 2156 2243 2261 1348 Size: 766 42 87 18 4542 Fragments arranged by size: 4542 766 87 42 18 PciI A'CATG_T Cuts at: 4354 4354 Size: 5455 PflMI CCAn_nnn'nTGG Cuts at: 1108 2279 1108 Size: 1171 4284 PfoI T'CCnGG_A Cuts at: 2167 2215 2167 Size: 48 5407 PmeI GTTT'AAAC Cuts at: 2759 2759 Size: 5455 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3524 3556 5037 5069 3524 Size: 32 1481 32 3910 Fragments arranged by size: 3910 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5455 PsiI TTA'TAA Cuts at: 2358 3025 2358 Size: 667 4788 PstI C_TGCA'G Cuts at: 830 2807 830 Size: 1977 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5455 SalI G'TCGA_C Cuts at: 1936 2797 1936 Size: 861 4594 SbfI CC_TGCA'GG Cuts at: 2807 2807 Size: 5455 ScaI AGT'ACT Cuts at: 2610 3703 5415 2610 Size: 1093 1712 2650 Fragments arranged by size: 2650 1712 1093 SfcI C'TryA_G Cuts at: 826 1045 2803 3938 4619 4810 826 Size: 219 1758 1135 681 191 1471 Fragments arranged by size: 1758 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2188 2329 2188 Size: 141 5314 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5455 SmaI CCC'GGG Cuts at: 1872 2143 2782 1872 Size: 271 639 4545 Fragments arranged by size: 4545 639 271 SmlI C'TyrA_G Cuts at: 820 1017 1943 2061 3559 4460 4722 820 Size: 197 926 118 1498 901 262 1553 Fragments arranged by size: 1553 1498 926 901 262 197 118 SnaBI TAC'GTA Cuts at: 493 3246 493 Size: 2753 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5455 SphI G_CATG'C Cuts at: 1132 2395 2813 1132 Size: 1263 418 3774 Fragments arranged by size: 3774 1263 418 SspI AAT'ATT Cuts at: 5 52 3379 5 Size: 47 3327 2081 Fragments arranged by size: 3327 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5455 StyI C'CwwG_G Cuts at: 513 1065 2004 2894 513 Size: 552 939 890 3074 Fragments arranged by size: 3074 939 890 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2266 3482 3499 3652 3837 4282 325 Size: 1255 686 1216 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1216 686 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2608 2664 3701 Size: 274 80 33 51 1443 631 56 1037 Cuts at: 3701 5413 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1037 631 274 138 80 56 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5415 5447 5415 Size: 32 5423 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5455 XbaI T'CTAG_A Cuts at: 2791 2791 Size: 5455 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5455 XmaI C'CCGG_G Cuts at: 1870 2141 2780 1870 Size: 271 639 4545 Fragments arranged by size: 4545 639 271 XmnI GAAnn'nnTTC Cuts at: 2594 3584 2594 Size: 990 4465 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3263 276 Size: 53 83 186 936 1729 2468 Fragments arranged by size: 2468 1729 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI