Restriction map for pFN21AB6301
 (Circular) (Six-base) MAPSORT of: pFN21AB6301.seq  Check: 6347  from: 1  to: 5455



pFN21AB6301  5455 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2233    2233 
   Size:       5455
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3265     278 
   Size:         53      83     186     936    1729    2468

  Fragments arranged by size: 

                2468    1729     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2238    2798    1937 
   Size:        301     560    4594

  Fragments arranged by size: 

                4594     560     301
 
Acc65I G'GTAC_C

Cuts at:   2776    2776 
   Size:       5455
 
AclI AA'CG_TT

Cuts at:   1563    3582    3955    1563 
   Size:       2019     373    3063

  Fragments arranged by size: 

                3063    2019     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3518    4902    5170    1632 
   Size:       1886    1384     268    1917

  Fragments arranged by size: 

                1917    1886    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5258
 
AflIII A'CryG_T

Cuts at:   1203    4354    5331    1203 
   Size:       3151     977    1327

  Fragments arranged by size: 

                3151    1327     977
 
AgeI A'CCGG_T

Cuts at:   4286    4286 
   Size:       5455
 
AhdI GACnn_n'nnGTC

Cuts at:   4184    4184 
   Size:       5455
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5455
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5455
 
AlwNI CAG_nnn'CTG

Cuts at:   3198    4770    3198 
   Size:       1572    3883
 
ApaLI G'TGCA_C

Cuts at:   3512    4668    3512 
   Size:       1156    4299
 
ApoI r'AATT_y

Cuts at:   1493    2709    2764    2990    3142    5251    1493 
   Size:       1216      55     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1216     226     152      55
 
AseI AT'TA_AT

Cuts at:    160    1032    4009     160 
   Size:        872    2977    1606

  Fragments arranged by size: 

                2977    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5455
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2141    2780    1870 
   Size:         73     198     639    4545

  Fragments arranged by size: 

                4545     639     198      73
 
BamHI G'GATC_C

Cuts at:   2785    3152    2785 
   Size:        367    5088
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2149    2176    2186    2327    2383 
   Size:        325     217     627     362      27      10     141      56

Cuts at:   2383    2776    4231     618 
   Size:        393    1455    1842

  Fragments arranged by size: 

                1842    1455     627     393     362     325     217     141
                  56      27      10
 
BanII G_rGCy'C

Cuts at:   1391    2774    1391 
   Size:       1383    4072
 
BbeI G_GCGC'C

Cuts at:   2190    2331    2190 
   Size:        141    5314
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4484
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3646    3680    1903 
   Size:         34    1709      34    3678

  Fragments arranged by size: 

                3678    1709      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3347    4557    3347 
   Size:       1210    4245
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5455
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2325    4066     136 
   Size:        107     122      71     631     605     653    1741    1525

  Fragments arranged by size: 

                1741    1525     653     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5450    5450 
   Size:       5455
 
BlpI GC'TnA_GC

Cuts at:   2872    2872 
   Size:       5455
 
Bme1580I G_kGCm'C

Cuts at:   1790    2414    3516    4672    1790 
   Size:        624    1102    1156    2573

  Fragments arranged by size: 

                2573    1156    1102     624
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5455
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2554    2714    3186 
   Size:        416      53      83     399      24    1137     160     472

Cuts at:   3186    4144     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1137     958     472     416     399     160      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5455
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2166    2217    2226    2253    4115    1239 
   Size:        927      51       9      27    1862    2579

  Fragments arranged by size: 

                2579    1862     927      51      27       9
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2046    3580    4445    4743    2046 
   Size:       1534     865     298    2758

  Fragments arranged by size: 

                2758    1534     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4118     882 
   Size:        617    2619    2219

  Fragments arranged by size: 

                2619    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3246     493 
   Size:        711    2042    2702

  Fragments arranged by size: 

                2702    2042     711
 
BsaBI GATnn'nnATC

Cuts at:   5449    5449 
   Size:       5455
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2187    2328    3262    3644 
   Size:         53      83     186     936     654     141     934     382

Cuts at:   3644    4279     275 
   Size:        635    1451

  Fragments arranged by size: 

                1451     936     934     654     635     382     186     141
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   2297    3888    4286    4560    4707    5118    2297 
   Size:       1591     398     274     147     411    2634

  Fragments arranged by size: 

                2634    1591     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5230

  Fragments arranged by size: 

                5230     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5455
 
BseYI C'CCAG_C

Cuts at:   2096    2252    2336    4658    5154    2096 
   Size:        156      84    2322     496    2397

  Fragments arranged by size: 

                2397    2322     496     156      84
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2211    1163 
   Size:        573     475    4407

  Fragments arranged by size: 

                4407     573     475
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2905    3666    4694    1540 
   Size:        275     186     904     761    1028    2301

  Fragments arranged by size: 

                2301    1028     904     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2203    2224    3516    3601    4672    2203 
   Size:         21    1292      85    1071    2986

  Fragments arranged by size: 

                2986    1292    1071      85      21
 
BsmI GAATG_Cn'

Cuts at:   2966    3059    2966 
   Size:         93    5362
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2203    2224    2414    2774    3516    3601    4672 
   Size:        399     413      21     190     360     742      85    1071

Cuts at:   4672    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     413     399     360     190      85
                  21
 
BspEI T'CCGG_A

Cuts at:   5118    5118 
   Size:       5455
 
BspHI T'CATG_A

Cuts at:   3344    5074    3344 
   Size:       1730    3725
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2233    2370    2810     844 
   Size:       1389     137     440    3489

  Fragments arranged by size: 

                3489    1389     440     137
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3342    1394 
   Size:        429    1519    3507

  Fragments arranged by size: 

                3507    1519     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3950    4124      65 
   Size:       1183    2702     174    1396

  Fragments arranged by size: 

                2702    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4099    4286    1700 
   Size:        110    2289     187    2869

  Fragments arranged by size: 

                2869    2289     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5455
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5455
 
BssSI C'ACGA_G

Cuts at:   3515    4527    3515 
   Size:       1012    4443
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5455
 
BstBI TT'CG_AA

Cuts at:   4259    4259 
   Size:       5455
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5455
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2096    2096 
   Size:       5455
 
BstYI r'GATC_y

Cuts at:   1972    2785    3152    3537    3554    5006    5092    5104    5450 
   Size:        813     367     385      17    1452      86      12     346

Cuts at:   5450    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     813     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3964

  Fragments arranged by size: 

                3964     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4716

  Fragments arranged by size: 

                4716     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2646    2971    3765    3785    2646 
   Size:        325     794      20    4316

  Fragments arranged by size: 

                4316     794     325      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5455
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5420
 
DraI TTT'AAA

Cuts at:   2759    2759 
   Size:       5455
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4462     809 
   Size:        507    3146    1802

  Fragments arranged by size: 

                3146    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2902    3791    5420 
   Size:         54    1425      50     275      18    1072     889    1629

Cuts at:   5420       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1072     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2902    1537 
   Size:        275    1090    4090

  Fragments arranged by size: 

                4090    1090     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3385    1353 
   Size:       2032    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4042    4416    4562     387 
   Size:       1064     138    2453     374     146    1280

  Fragments arranged by size: 

                2453    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2166    2217    2226    2253    3518    4115    4902 
   Size:        393     534      51       9      27    1265     597     787

Cuts at:   4902    5170    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1265     787     597     534     393     268      51
                  27       9
 
EcoO109I rG'GnC_Cy

Cuts at:   2133    2265    2133 
   Size:        132    5323
 
EcoRI G'AATT_C

Cuts at:   2764    3142    2764 
   Size:        378    5077
 
FspI TGC'GCA

Cuts at:   3961    3961 
   Size:       5455
 
HaeII r_GCGC'y

Cuts at:   1678    2190    2331    4602    1678 
   Size:        512     141    2271    2531

  Fragments arranged by size: 

                2531    2271     512     141
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2021    2053    4096    4128    4170 
   Size:         32     135      32     449      32    2043      32      42

Cuts at:   4170    4202    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    2043     449     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2799    3045     669 
   Size:        988     281     861     246    3079

  Fragments arranged by size: 

                3079     988     861     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5455
 
HpaI GTT'AAC

Cuts at:   3045    3045 
   Size:       5455
 
KasI G'GCGC_C

Cuts at:   2186    2327    2186 
   Size:        141    5314
 
KpnI G_GTAC'C

Cuts at:   2780    2780 
   Size:       5455
 
MfeI C'AATT_G

Cuts at:   3054    3054 
   Size:       5455
 
MluI A'CGCG_T

Cuts at:   5331    5331 
   Size:       5455
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4569    4753    1305 
   Size:       3264     184    2007

  Fragments arranged by size: 

                3264    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3633

  Fragments arranged by size: 

                3633    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3413    3772    3931     518 
   Size:        648    2247     359     159    2042

  Fragments arranged by size: 

                2247    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2256    2871    3548    4696    4941    1606 
   Size:        650     615     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     650     615     245
 
NarI GG'CG_CC

Cuts at:   2187    2328    2187 
   Size:        141    5314
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3964

  Fragments arranged by size: 

                3964     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5455
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5455
 
NotI GC'GGCC_GC

Cuts at:   2902    2902 
   Size:       5455
 
NspI r_CATG'y

Cuts at:   1132    1229    2395    2813    4358    1132 
   Size:         97    1166     418    1545    2229

  Fragments arranged by size: 

                2229    1545    1166     418      97
 
PasI CC'CwG_GG

Cuts at:   1348    2114    2156    2243    2261    1348 
   Size:        766      42      87      18    4542

  Fragments arranged by size: 

                4542     766      87      42      18
 
PciI A'CATG_T

Cuts at:   4354    4354 
   Size:       5455
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2279    1108 
   Size:       1171    4284
 
PfoI T'CCnGG_A

Cuts at:   2167    2215    2167 
   Size:         48    5407
 
PmeI GTTT'AAAC

Cuts at:   2759    2759 
   Size:       5455
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3524    3556    5037    5069    3524 
   Size:         32    1481      32    3910

  Fragments arranged by size: 

                3910    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5455
 
PsiI TTA'TAA

Cuts at:   2358    3025    2358 
   Size:        667    4788
 
PstI C_TGCA'G

Cuts at:    830    2807     830 
   Size:       1977    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5455
 
SalI G'TCGA_C

Cuts at:   1936    2797    1936 
   Size:        861    4594
 
SbfI CC_TGCA'GG

Cuts at:   2807    2807 
   Size:       5455
 
ScaI AGT'ACT

Cuts at:   2610    3703    5415    2610 
   Size:       1093    1712    2650

  Fragments arranged by size: 

                2650    1712    1093
 
SfcI C'TryA_G

Cuts at:    826    1045    2803    3938    4619    4810     826 
   Size:        219    1758    1135     681     191    1471

  Fragments arranged by size: 

                1758    1471    1135     681     219     191
 
SfoI GGC'GCC

Cuts at:   2188    2329    2188 
   Size:        141    5314
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5455
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5455
 
SmaI CCC'GGG

Cuts at:   1872    2143    2782    1872 
   Size:        271     639    4545

  Fragments arranged by size: 

                4545     639     271
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2061    3559    4460    4722     820 
   Size:        197     926     118    1498     901     262    1553

  Fragments arranged by size: 

                1553    1498     926     901     262     197     118
 
SnaBI TAC'GTA

Cuts at:    493    3246     493 
   Size:       2753    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5455
 
SphI G_CATG'C

Cuts at:   1132    2395    2813    1132 
   Size:       1263     418    3774

  Fragments arranged by size: 

                3774    1263     418
 
SspI AAT'ATT

Cuts at:      5      52    3379       5 
   Size:         47    3327    2081

  Fragments arranged by size: 

                3327    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5455
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2894     513 
   Size:        552     939     890    3074

  Fragments arranged by size: 

                3074     939     890     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2266    3482    3499    3652    3837    4282     325 
   Size:       1255     686    1216      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1216     686     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2608    2664    3701 
   Size:        274      80      33      51    1443     631      56    1037

Cuts at:   3701    5413      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1037     631     274     138      80      56
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5415    5447    5415 
   Size:         32    5423
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5455
 
XbaI T'CTAG_A

Cuts at:   2791    2791 
   Size:       5455
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5455
 
XmaI C'CCGG_G

Cuts at:   1870    2141    2780    1870 
   Size:        271     639    4545

  Fragments arranged by size: 

                4545     639     271
 
XmnI GAAnn'nnTTC

Cuts at:   2594    3584    2594 
   Size:        990    4465
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3263     276 
   Size:         53      83     186     936    1729    2468

  Fragments arranged by size: 

                2468    1729     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI
     PstI     PvuI     SalI     SbfI     ScaI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BbvCI    BfrBI
     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI    EcoRV     FalI     FseI    FspAI     NaeI   NgoMIV     NruI
     NsiI     PacI     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII
    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI     SrfI
     SwaI     XcmI
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