(Linear) (Six-base) MAPSORT of: KIEE4440.seq Check: 4502 from: 1 to: 1371 KIEE4440.orf With 182 enzymes: SgfI * March 22, 2012 18:02 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 1131 1371 Size: 247 884 240 Fragments arranged by size: 884 247 240 AleI CACnn'nnGTG Cuts at: 0 754 1371 Size: 754 617 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 65 1371 Size: 65 1306 AlwNI CAG_nnn'CTG Cuts at: 0 627 1299 1371 Size: 627 672 72 Fragments arranged by size: 672 627 72 ApaI G_GGCC'C Cuts at: 0 1134 1156 1371 Size: 1134 22 215 Fragments arranged by size: 1134 215 22 ApoI r'AATT_y Cuts at: 0 219 1371 Size: 219 1152 AvaI C'yCGr_G Cuts at: 0 733 771 1133 1193 1243 1371 Size: 733 38 362 60 50 128 Fragments arranged by size: 733 362 128 60 50 38 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 77 110 1371 Size: 77 33 1261 Fragments arranged by size: 1261 77 33 BamHI G'GATC_C Cuts at: 0 194 1371 Size: 194 1177 BanI G'GyrC_C Cuts at: 0 256 1371 Size: 256 1115 BanII G_rGCy'C Cuts at: 0 236 470 1134 1156 1371 Size: 236 234 664 22 215 Fragments arranged by size: 664 236 234 215 22 BbeI G_GCGC'C Cuts at: 0 260 1371 Size: 260 1111 BbvCI CC'TCA_GC Cuts at: 0 488 710 854 1371 Size: 488 222 144 517 Fragments arranged by size: 517 488 222 144 BciVI GTATCCnnnnn_n' Cuts at: 0 78 1371 Size: 78 1293 BfrBI ATG'CAT Cuts at: 0 104 1371 Size: 104 1267 BglI GCCn_nnn'nGGC Cuts at: 0 666 1321 1371 Size: 666 655 50 Fragments arranged by size: 666 655 50 Bme1580I G_kGCm'C Cuts at: 0 273 1134 1156 1371 Size: 273 861 22 215 Fragments arranged by size: 861 273 215 22 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 135 633 1011 1371 Size: 135 498 378 360 Fragments arranged by size: 498 378 360 135 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 539 737 994 1044 1093 1200 1210 1321 Size: 539 198 257 50 49 107 10 111 Cuts at: 1321 1351 1371 Size: 30 20 Fragments arranged by size: 539 257 198 111 107 50 49 30 20 10 Bpu10I CC'TnA_GC Cuts at: 0 488 710 854 863 1371 Size: 488 222 144 9 508 Fragments arranged by size: 508 488 222 144 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 1228 1371 Size: 343 885 143 Fragments arranged by size: 885 343 143 BsaHI Gr'CG_yC Cuts at: 0 257 1371 Size: 257 1114 BsaWI w'CCGG_w Cuts at: 0 794 1371 Size: 794 577 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 537 567 1294 1324 1371 Size: 537 30 727 30 47 Fragments arranged by size: 727 537 47 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 197 535 538 541 544 547 700 703 Size: 197 338 3 3 3 3 153 3 Cuts at: 703 706 709 712 715 718 736 871 925 Size: 3 3 3 3 3 18 135 54 Cuts at: 925 928 931 934 997 1000 1099 1102 1105 Size: 3 3 3 63 3 99 3 3 Cuts at: 1105 1261 1273 1276 1371 Size: 156 12 3 95 Fragments arranged by size: 338 197 156 153 135 99 95 63 54 18 12 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 139 568 838 899 1371 Size: 139 429 270 61 472 Fragments arranged by size: 472 429 270 139 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 674 1371 Size: 674 697 BsiEI CG_ry'CG Cuts at: 0 814 1371 Size: 814 557 BsiHKAI G_wGCw'C Cuts at: 0 236 896 1371 Size: 236 660 475 Fragments arranged by size: 660 475 236 Bsp1286I G_dGCh'C Cuts at: 0 236 273 470 896 1134 1156 1371 Size: 236 37 197 426 238 22 215 Fragments arranged by size: 426 238 236 215 197 37 22 BspEI T'CCGG_A Cuts at: 0 794 1371 Size: 794 577 BsrBI CCG'CTC Cuts at: 0 965 990 1371 Size: 965 25 381 Fragments arranged by size: 965 381 25 BstYI r'GATC_y Cuts at: 0 194 791 1371 Size: 194 597 580 Fragments arranged by size: 597 580 194 BtsI GCAGTG_nn' Cuts at: 0 47 935 1371 Size: 47 888 436 Fragments arranged by size: 888 436 47 EaeI y'GGCC_r Cuts at: 0 1043 1313 1371 Size: 1043 270 58 Fragments arranged by size: 1043 270 58 EarI CTCTTCn'nnn_ Cuts at: 0 29 359 512 677 952 955 1371 Size: 29 330 153 165 275 3 416 Fragments arranged by size: 416 330 275 165 153 29 3 EcoICRI GAG'CTC Cuts at: 0 234 1371 Size: 234 1137 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 539 737 994 1044 1093 1131 1200 Size: 247 292 198 257 50 49 38 69 Cuts at: 1200 1210 1321 1351 1371 Size: 10 111 30 20 Fragments arranged by size: 292 257 247 198 111 69 50 49 38 30 20 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 619 1371 Size: 619 752 EcoO109I rG'GnC_Cy Cuts at: 0 449 456 504 612 645 745 867 1152 Size: 449 7 48 108 33 100 122 285 Cuts at: 1152 1153 1371 Size: 1 218 Fragments arranged by size: 449 285 218 122 108 100 48 33 7 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1140 1371 Size: 1140 231 HaeII r_GCGC'y Cuts at: 0 260 1371 Size: 260 1111 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 258 290 295 327 358 390 796 828 Size: 258 32 5 32 31 32 406 32 Cuts at: 828 1294 1326 1371 Size: 466 32 45 Fragments arranged by size: 466 406 258 45 32 32 32 32 32 31 5 KasI G'GCGC_C Cuts at: 0 256 1371 Size: 256 1115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 146 794 1371 Size: 146 648 577 Fragments arranged by size: 648 577 146 MscI TGG'CCA Cuts at: 0 1045 1371 Size: 1045 326 MslI CAynn'nnrTG Cuts at: 0 754 1371 Size: 754 617 MspA1I CmG'CkG Cuts at: 0 13 293 1041 1167 1350 1371 Size: 13 280 748 126 183 21 Fragments arranged by size: 748 280 183 126 21 13 NarI GG'CG_CC Cuts at: 0 257 1371 Size: 257 1114 NsiI A_TGCA'T Cuts at: 0 106 1371 Size: 106 1265 PasI CC'CwG_GG Cuts at: 0 508 1156 1371 Size: 508 648 215 Fragments arranged by size: 648 508 215 PflMI CCAn_nnn'nTGG Cuts at: 0 1004 1022 1299 1371 Size: 1004 18 277 72 Fragments arranged by size: 1004 277 72 18 PfoI T'CCnGG_A Cuts at: 0 461 1371 Size: 461 910 PpuMI rG'GwC_Cy Cuts at: 0 867 1371 Size: 867 504 PspOMI G'GGCC_C Cuts at: 0 1130 1152 1371 Size: 1130 22 219 Fragments arranged by size: 1130 219 22 PstI C_TGCA'G Cuts at: 0 1355 1371 Size: 1355 16 PvuII CAG'CTG Cuts at: 0 1041 1167 1350 1371 Size: 1041 126 183 21 Fragments arranged by size: 1041 183 126 21 SacI G_AGCT'C Cuts at: 0 236 1371 Size: 236 1135 SapI GCTCTTCn'nnn_ Cuts at: 0 29 359 955 1371 Size: 29 330 596 416 Fragments arranged by size: 596 416 330 29 SfcI C'TryA_G Cuts at: 0 752 1351 1371 Size: 752 599 20 Fragments arranged by size: 752 599 20 SfoI GGC'GCC Cuts at: 0 258 1371 Size: 258 1113 SmaI CCC'GGG Cuts at: 0 1135 1195 1371 Size: 1135 60 176 Fragments arranged by size: 1135 176 60 SmlI C'TyrA_G Cuts at: 0 322 1207 1371 Size: 322 885 164 Fragments arranged by size: 885 322 164 SrfI GCCC'GGGC Cuts at: 0 1135 1371 Size: 1135 236 StuI AGG'CCT Cuts at: 0 944 1371 Size: 944 427 StyI C'CwwG_G Cuts at: 0 252 260 377 407 482 777 998 1046 Size: 252 8 117 30 75 295 221 48 Cuts at: 1046 1371 Size: 325 Fragments arranged by size: 325 295 252 221 117 75 48 30 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 562 800 1371 Size: 562 238 571 Fragments arranged by size: 571 562 238 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 537 569 1371 Size: 537 32 802 Fragments arranged by size: 802 537 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 384 775 1371 Size: 384 391 596 Fragments arranged by size: 596 391 384 XmaI C'CCGG_G Cuts at: 0 1133 1193 1371 Size: 1133 60 178 Fragments arranged by size: 1133 178 60 Enzymes that do cut: AcuI AleI AlfI AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII BbeI BbvCI BciVI BfrBI BglI Bme1580I BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BsrBI BstYI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI NsiI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SapI SfcI SfoI SmaI SmlI SrfI StuI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII BbsI BcgI BclI BglII BlpI BmgBI BmrI BmtI BsaI BsaAI BsaBI BsiWI BsmI BsmBI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI