################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII BbeI BbrPI BciVI BclI BfrI BglII BseAI BsiWI BsmI BspEI BspHI BspLU11I BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CpoI DraIII DrdI Eam1105I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI MfeI MluI MroI MunI NarI NdeI NheI NotI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI RsrII SalI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI StuI SwaI Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcyI 1517 GACGCC G[GA]^CG[CT]C AocI 1177 CCTGAGG CC^T[ACGT]AGG Aor51HI 1766 AGCGCT AGC^GCT ApaI 1147 GGGCCC GGGCC^C AspI 1322 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 567 CCTAGG C^CTAGG BalI 2036 TGGCCA TGG^CCA BamHI 1629 GGATCC G^GATCC BlnI 567 CCTAGG C^CTAGG BsaAI 2134 TACGTG [CT]AC^GT[GA] BsaHI 1517 GACGCC G[GA]^CG[CT]C BsaI 1954 GAGACC GGTCTC(1/5) BsaWI 119 ACCGGA [AT]^CCGG[AT] BsgI 1963 GTGCAG GTGCAG(16/14) BsmBI 2143 GAGACG CGTCTC(1/5) Bsp1407I 743 TGTACA T^GTACA BspDI 2041 ATCGAT AT^CGAT BsrGI 743 TGTACA T^GTACA BsrI 669 ACTGG ACTGG(1/-1) BstEII 1188 GGTGACC G^GT[ACGT]ACC BstPI 1188 GGTGACC G^GT[ACGT]ACC Bsu36I 1177 CCTGAGG CC^T[ACGT]AGG ClaI 2041 ATCGAT AT^CGAT DraI 1195 TTTAAA TTT^AAA Eco47III 1766 AGCGCT AGC^GCT Eco81I 1177 CCTGAGG CC^T[ACGT]AGG EcoO65I 1188 GGTGACC G^GT[ACGT]ACC HgaI 1517 GACGC GACGC(5/10) Hin1I 1517 GACGCC G[GA]^CG[CT]C KspI 88 CCGCGG CCGC^GG MluNI 2036 TGGCCA TGG^CCA MscI 2036 TGGCCA TGG^CCA NcoI 2199 CCATGG C^CATGG NruI 1722 TCGCGA TCG^CGA NspI 780 GCATGC [GA]CATG^[CT] PflFI 1322 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 543 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuI 1895 CGATCG CGAT^CG SacII 88 CCGCGG CCGC^GG SapI 1015 GAAGAGC GCTCTTC(1/4) SbfI 759 CCTGCAGG CCTGCA^GG SexAI 878 ACCTGGT A^CC[AT]GGT SmlI 826 CTCAAG C^T[CT][GA]AG SphI 780 GCATGC GCATG^C Sse8387I 759 CCTGCAGG CCTGCA^GG SspBI 743 TGTACA T^GTACA Tth111I 1322 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 543 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BbsI 2223 GAAGAC GAAGAC(2/6) 2337 GAAGAC BcgI 306 CGAGGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 981 GCAAAAACCTCG BfaI 329 CTAG C^TAG 568 CTAG BglI 84 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1604 GCCAGCGGGGC BlpI 1769 GCTCAGC GC^T[ACGT]AGC 2355 GCTCAGC Bpu1102I 1769 GCTCAGC GC^T[ACGT]AGC 2355 GCTCAGC BpuAI 2223 GAAGAC GAAGAC(2/6) 2337 GAAGAC BsaBI 1624 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2034 GATGGCCATC CelII 1769 GCTCAGC GC^T[ACGT]AGC 2355 GCTCAGC EagI 38 CGGCCG C^GGCCG 56 CGGCCG EarI 654 GAAGAG CTCTTC(1/4) 1015 GAAGAG EclXI 38 CGGCCG C^GGCCG 56 CGGCCG Eco52I 38 CGGCCG C^GGCCG 56 CGGCCG Ksp632I 654 GAAGAG CTCTTC(1/4) 1015 GAAGAG MaeI 329 CTAG C^TAG 568 CTAG MamI 1624 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2034 GATGGCCATC PleI 1287 GACTC GAGTC(4/5) 1597 GAGTC SmaI 752 CCCGGG CCC^GGG 1976 CCCGGG Tsp45I 451 GTGAC ^GT[GC]AC 1189 GTGAC XmaCI 752 CCCGGG C^CCGGG 1976 CCCGGG XmaI 752 CCCGGG C^CCGGG 1976 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BmyI BpmI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI TspRI TthHB8I XcmI XhoII