(Linear) (Six-base) MAPSORT of: KIEE1474.seq Check: 2268 from: 1 to: 1506 KIEE1474.orf With 182 enzymes: SgfI * July 3, 2012 16:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1443 1506 Size: 1443 63 AatII G_ACGT'C Cuts at: 0 1199 1506 Size: 1199 307 AccI GT'mk_AC Cuts at: 0 1337 1506 Size: 1337 169 Acc65I G'GTAC_C Cuts at: 0 1216 1506 Size: 1216 290 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 683 1506 Size: 683 823 AleI CACnn'nnGTG Cuts at: 0 907 1506 Size: 907 599 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1160 1506 Size: 1160 346 AlwNI CAG_nnn'CTG Cuts at: 0 718 779 1385 1506 Size: 718 61 606 121 Fragments arranged by size: 718 606 121 61 ApaI G_GGCC'C Cuts at: 0 414 1506 Size: 414 1092 ApoI r'AATT_y Cuts at: 0 465 1057 1506 Size: 465 592 449 Fragments arranged by size: 592 465 449 AvaI C'yCGr_G Cuts at: 0 50 123 1506 Size: 50 73 1383 Fragments arranged by size: 1383 73 50 BamHI G'GATC_C Cuts at: 0 540 1506 Size: 540 966 BanI G'GyrC_C Cuts at: 0 1216 1506 Size: 1216 290 BanII G_rGCy'C Cuts at: 0 414 1506 Size: 414 1092 BbsI GAAGACnn'nnnn_ Cuts at: 0 964 1170 1506 Size: 964 206 336 Fragments arranged by size: 964 336 206 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 630 664 1354 1388 1506 Size: 630 34 690 34 118 Fragments arranged by size: 690 630 118 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 1490 1506 Size: 1490 16 BlpI GC'TnA_GC Cuts at: 0 16 1506 Size: 16 1490 Bme1580I G_kGCm'C Cuts at: 0 414 1506 Size: 414 1092 BmrI ACTGGGnnnn_n' Cuts at: 0 92 728 1238 1506 Size: 92 636 510 268 Fragments arranged by size: 636 510 268 92 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 77 1506 Size: 77 1429 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 1241 1506 Size: 153 1088 265 Fragments arranged by size: 1088 265 153 BsaAI yAC'GTr Cuts at: 0 1329 1506 Size: 1329 177 BsaHI Gr'CG_yC Cuts at: 0 824 1196 1506 Size: 824 372 310 Fragments arranged by size: 824 372 310 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 585 615 1506 Size: 585 30 891 Fragments arranged by size: 891 585 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 586 868 1506 Size: 586 282 638 Fragments arranged by size: 638 586 282 BseYI C'CCAG_C Cuts at: 0 206 1506 Size: 206 1300 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 54 763 1156 1303 1448 1506 Size: 54 709 393 147 145 58 Fragments arranged by size: 709 393 147 145 58 54 BsiEI CG_ry'CG Cuts at: 0 62 1506 Size: 62 1444 BsiHKAI G_wGCw'C Cuts at: 0 809 1506 Size: 809 697 Bsp1286I G_dGCh'C Cuts at: 0 414 809 1506 Size: 414 395 697 Fragments arranged by size: 697 414 395 BspHI T'CATG_A Cuts at: 0 1403 1506 Size: 1403 103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1443 1506 Size: 1443 63 BsrDI GCAATG_nn' Cuts at: 0 1071 1506 Size: 1071 435 BssSI C'ACGA_G Cuts at: 0 7 1506 Size: 7 1499 BstAPI GCAn_nnn'nTGC Cuts at: 0 779 1506 Size: 779 727 BstXI CCAn_nnnn'nTGG Cuts at: 0 606 1139 1506 Size: 606 533 367 Fragments arranged by size: 606 533 367 BstYI r'GATC_y Cuts at: 0 540 923 1506 Size: 540 383 583 Fragments arranged by size: 583 540 383 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1226 1506 Size: 1226 280 BtsI GCAGTG_nn' Cuts at: 0 551 1144 1299 1415 1506 Size: 551 593 155 116 91 Fragments arranged by size: 593 551 155 116 91 DrdI GACnn_nn'nnGTC Cuts at: 0 1174 1506 Size: 1174 332 EaeI y'GGCC_r Cuts at: 0 23 899 1506 Size: 23 876 607 Fragments arranged by size: 876 607 23 EarI CTCTTCn'nnn_ Cuts at: 0 1270 1506 Size: 1270 236 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 77 683 1506 Size: 77 606 823 Fragments arranged by size: 823 606 77 EcoNI CCTnn'n_nnAGG Cuts at: 0 1405 1506 Size: 1405 101 EcoO109I rG'GnC_Cy Cuts at: 0 138 410 1506 Size: 138 272 1096 Fragments arranged by size: 1096 272 138 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 981 1506 Size: 981 525 HaeII r_GCGC'y Cuts at: 0 44 1506 Size: 44 1462 HincII GTy'rAC Cuts at: 0 1200 1506 Size: 1200 306 KpnI G_GTAC'C Cuts at: 0 1220 1506 Size: 1220 286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 657 810 1506 Size: 657 153 696 Fragments arranged by size: 696 657 153 MscI TGG'CCA Cuts at: 0 901 1506 Size: 901 605 MslI CAynn'nnrTG Cuts at: 0 460 907 1506 Size: 460 447 599 Fragments arranged by size: 599 460 447 MspA1I CmG'CkG Cuts at: 0 293 747 1506 Size: 293 454 759 Fragments arranged by size: 759 454 293 PasI CC'CwG_GG Cuts at: 0 606 1506 Size: 606 900 PflMI CCAn_nnn'nTGG Cuts at: 0 718 1506 Size: 718 788 PpuMI rG'GwC_Cy Cuts at: 0 138 1506 Size: 138 1368 PspOMI G'GGCC_C Cuts at: 0 410 1506 Size: 410 1096 PstI C_TGCA'G Cuts at: 0 1294 1506 Size: 1294 212 PvuII CAG'CTG Cuts at: 0 293 747 1506 Size: 293 454 759 Fragments arranged by size: 759 454 293 SfcI C'TryA_G Cuts at: 0 288 380 1290 1506 Size: 288 92 910 216 Fragments arranged by size: 910 288 216 92 SmaI CCC'GGG Cuts at: 0 125 1506 Size: 125 1381 SmlI C'TyrA_G Cuts at: 0 132 1256 1506 Size: 132 1124 250 Fragments arranged by size: 1124 250 132 SpeI A'CTAG_T Cuts at: 0 163 1506 Size: 163 1343 SspI AAT'ATT Cuts at: 0 420 1506 Size: 420 1086 StuI AGG'CCT Cuts at: 0 319 1506 Size: 319 1187 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 897 1506 Size: 897 609 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 585 617 788 820 1506 Size: 585 32 171 32 686 Fragments arranged by size: 686 585 171 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1171 1506 Size: 1171 335 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 95 909 1506 Size: 95 814 597 Fragments arranged by size: 814 597 95 XmaI C'CCGG_G Cuts at: 0 123 1506 Size: 123 1383 XmnI GAAnn'nnTTC Cuts at: 0 1061 1506 Size: 1061 445 ZraI GAC'GTC Cuts at: 0 1197 1506 Size: 1197 309 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AleI AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BcgI BglI BlpI Bme1580I BmrI BpmI BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI BssSI BstAPI BstXI BstYI BtgZI BtsI DrdI EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII HincII KpnI MmeI MscI MslI MspA1I PasI PflMI PpuMI PspOMI PstI PvuII SfcI SmaI SmlI SpeI SspI StuI TaqII TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AclI AfeI AflII AflIII AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BciVI BclI BfrBI BglII BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I HindIII HpaI KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SphI SrfI StyI SwaI TatI XbaI XhoI