################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeII MfeI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AcyI 118 GGCGTC G[GA]^CG[CT]C Alw44I 244 GTGCAC G^TGCAC AlwNI 664 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 672 GGGCCC GGGCC^C ApaLI 244 GTGCAC G^TGCAC Asp700I 516 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 244 GTGCAC G[AT]GC[AT]^C BalI 584 TGGCCA TGG^CCA BamHI 51 GGATCC G^GATCC BanI 93 GGTGCC G^G[CT][GA]CC BfaI 145 CTAG C^TAG BglI 232 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 875 AGATCT A^GATCT BpmI 804 CTCCAG CTGGAG(16/14) BsaBI 876 GATCTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 118 GGCGTC G[GA]^CG[CT]C BsaI 759 GGTCTC GGTCTC(1/5) BseRI 643 GAGGAG GAGGAG(10/8) BsiHKAI 244 GTGCAC G[AT]GC[AT]^C BsmAI 760 GTCTC GTCTC(1/5) Bsp1407I 2 TGTACA T^GTACA BspHI 317 TCATGA T^CATGA BspMI 930 GCAGGT ACCTGC(4/8) BsrDI 299 GCAATG GCAATG(2/0) BsrFI 90 GCCGGT [GA]^CCGG[CT] BsrGI 2 TGTACA T^GTACA Cfr10I 90 GCCGGT [GA]^CCGG[CT] DsaI 331 CCACGG C^C[GA][CT]GG EcoNI 786 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 831 GATATC GAT^ATC EcoT14I 791 CCAAGG C^C[AT][AT]GG HgaI 119 GCGTC GACGC(5/10) Hin1I 118 GGCGTC G[GA]^CG[CT]C HphI 423 GGTGA GGTGA(8/7) MaeI 145 CTAG C^TAG MaeIII 631 GTCAC ^GT[ACGT]AC MamI 876 GATCTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 584 TGGCCA TGG^CCA MscI 584 TGGCCA TGG^CCA MslI 975 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PflMI 663 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 928 CTGCAG CTGCA^G RcaI 317 TCATGA T^CATGA SbfI 927 CCTGCAGG CCTGCA^GG SexAI 419 ACCAGGT A^CC[AT]GGT SfaNI 766 GATGC GCATC(5/9) SfcI 928 CTGCAG C^T[GA][CT]AG SmaI 209 CCCGGG CCC^GGG SmlI 340 CTCAAG C^T[CT][GA]AG SphI 956 GCATGC GCATG^C Sse8387I 927 CCTGCAGG CCTGCA^GG SspBI 2 TGTACA T^GTACA StuI 57 AGGCCT AGG^CCT StyI 791 CCAAGG C^C[AT][AT]GG TfiI 987 GATTC G^A[AT]TC Tsp45I 631 GTCAC ^GT[GC]AC Tsp509I 1025 AATT ^AATT Van91I 663 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 209 CCCGGG C^CCGGG XmaI 209 CCCGGG C^CCGGG XmnI 516 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 286 CGCG CG^CG 953 CGCG AocI 703 CCTGAGG CC^T[ACGT]AGG 994 CCTCAGG AvaI 129 CTCGGG C^[CT]CG[GA]G 209 CCCGGG BbsI 709 GAAGAC GAAGAC(2/6) 121 GTCTTC BpuAI 709 GAAGAC GAAGAC(2/6) 121 GTCTTC BsoBI 129 CTCGGG C^[CT]CG[GA]G 209 CCCGGG BstUI 286 CGCG CG^CG 953 CGCG BstXI 348 CCAGGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 545 CCAACATCCTGG BstYI 51 GGATCC [GA]^GATC[CT] 875 AGATCT Bsu36I 703 CCTGAGG CC^T[ACGT]AGG 994 CCTCAGG CfoI 103 GCGC GCG^C 954 GCGC EaeI 584 TGGCCA [CT]^GGCC[GA] 935 TGGCCG EarI 313 CTCTTC CTCTTC(1/4) 166 GAAGAG Eco81I 703 CCTGAGG CC^T[ACGT]AGG 994 CCTCAGG FauI 72 GCGGG CCCGC(4/6) 456 GCGGG HhaI 103 GCGC GCG^C 954 GCGC HinP1I 103 GCGC G^CGC 954 GCGC Ksp632I 313 CTCTTC CTCTTC(1/4) 166 GAAGAG MflI 51 GGATCC [GA]^GATC[CT] 875 AGATCT MspA1I 398 CAGCTG C[AC]G^C[GT]G 719 CAGCTG MvnI 286 CGCG CG^CG 953 CGCG NspI 63 GCATGT [GA]CATG^[CT] 956 GCATGC PpuMI 572 GGGACCT [GA]G^G[AT]CC[CT] 665 AGGTCCT PvuII 398 CAGCTG CAG^CTG 719 CAGCTG XhoII 51 GGATCC [GA]^GATC[CT] 875 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BanII BbvI BcnI BmyI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI TseI TspRI