(Circular) (Six-base) MAPSORT of: pFN21AE9615.seq Check: 3413 from: 1 to: 5749 pFN21AE9615 5749 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2926 2926 Size: 5749 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3559 278 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3092 1937 Size: 1155 4594 Acc65I G'GTAC_C Cuts at: 3070 3070 Size: 5749 AclI AA'CG_TT Cuts at: 1563 3876 4249 1563 Size: 2313 373 3063 Fragments arranged by size: 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2111 2714 3812 5196 5464 1632 Size: 479 603 1098 1384 268 1917 Fragments arranged by size: 1917 1384 1098 603 479 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5552 AflIII A'CryG_T Cuts at: 1203 4648 5625 1203 Size: 3445 977 1327 Fragments arranged by size: 3445 1327 977 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 4478 4478 Size: 5749 AleI CACnn'nnGTG Cuts at: 1166 2984 1166 Size: 1818 3931 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5749 AlwNI CAG_nnn'CTG Cuts at: 2674 3492 5064 2674 Size: 818 1572 3359 Fragments arranged by size: 3359 1572 818 ApaI G_GGCC'C Cuts at: 2681 2681 Size: 5749 ApaLI G'TGCA_C Cuts at: 2249 3806 4962 2249 Size: 1557 1156 3036 Fragments arranged by size: 3036 1557 1156 ApoI r'AATT_y Cuts at: 1493 3058 3284 3436 5545 1493 Size: 1565 226 152 2109 1697 Fragments arranged by size: 2109 1697 1565 226 152 AseI AT'TA_AT Cuts at: 160 1032 4303 160 Size: 872 3271 1606 Fragments arranged by size: 3271 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 2134 2214 3074 1870 Size: 73 191 80 860 4545 Fragments arranged by size: 4545 860 191 80 73 BamHI G'GATC_C Cuts at: 2056 3079 3446 2056 Size: 1023 367 4359 Fragments arranged by size: 4359 1023 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2098 3070 4525 618 Size: 325 217 627 311 972 1455 1842 Fragments arranged by size: 1842 1455 972 627 325 311 217 BanII G_rGCy'C Cuts at: 1391 2401 2681 2871 3068 1391 Size: 1010 280 190 197 4072 Fragments arranged by size: 4072 1010 280 197 190 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2119 2721 928 Size: 971 220 602 3956 Fragments arranged by size: 3956 971 602 220 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3940 3974 1903 Size: 34 2003 34 3678 Fragments arranged by size: 3678 2003 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3641 4851 3641 Size: 1210 4539 BclI T'GATC_A Cuts at: 1548 1548 Size: 5749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2243 4360 136 Size: 107 122 71 631 605 571 2117 1525 Fragments arranged by size: 2117 1525 631 605 571 122 107 71 BglII A'GATC_T Cuts at: 2880 5744 2880 Size: 2864 2885 BlpI GC'TnA_GC Cuts at: 3166 3166 Size: 5749 Bme1580I G_kGCm'C Cuts at: 1790 2253 2467 2681 3810 4966 1790 Size: 463 214 214 1129 1156 2573 Fragments arranged by size: 2573 1156 1129 463 214 214 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2482 2499 3480 Size: 416 53 83 399 24 1065 17 981 Cuts at: 3480 4438 442 Size: 958 1753 Fragments arranged by size: 1753 1065 981 958 416 399 83 53 24 17 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5749 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2049 2049 Size: 5749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2794 4409 1239 Size: 1555 1615 2579 Fragments arranged by size: 2579 1615 1555 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2330 3874 4739 5037 2330 Size: 1544 865 298 3042 Fragments arranged by size: 3042 1544 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2770 4412 882 Size: 617 1271 1642 2219 Fragments arranged by size: 2219 1642 1271 617 BsaAI yAC'GTr Cuts at: 493 1204 3540 493 Size: 711 2336 2702 Fragments arranged by size: 2702 2336 711 BsaBI GATnn'nnATC Cuts at: 2885 5743 2885 Size: 2858 2891 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2124 3556 3938 4573 Size: 53 83 186 936 591 1432 382 635 Cuts at: 4573 275 Size: 1451 Fragments arranged by size: 1451 1432 936 635 591 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4182 4580 4854 5001 5412 4182 Size: 398 274 147 411 4519 Fragments arranged by size: 4519 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2372 2402 1178 Size: 30 165 30 969 30 4525 Fragments arranged by size: 4525 969 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2663 1186 Size: 1477 4272 BseYI C'CCAG_C Cuts at: 2266 2401 4952 5448 2266 Size: 135 2551 496 2567 Fragments arranged by size: 2567 2551 496 135 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2072 2141 2171 2303 2318 1163 Size: 573 336 69 30 132 15 4594 Fragments arranged by size: 4594 573 336 132 69 30 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3199 3960 4988 1540 Size: 275 186 1198 761 1028 2301 Fragments arranged by size: 2301 1198 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2253 3810 3895 4966 2253 Size: 1557 85 1071 3036 Fragments arranged by size: 3036 1557 1071 85 BsmI GAATG_Cn' Cuts at: 3260 3353 3260 Size: 93 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2253 2401 2467 2681 2871 3068 3810 Size: 399 463 148 66 214 190 197 742 Cuts at: 3810 3895 4966 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 463 399 214 197 190 148 85 66 BspEI T'CCGG_A Cuts at: 5412 5412 Size: 5749 BspHI T'CATG_A Cuts at: 2322 3638 5368 2322 Size: 1316 1730 2703 Fragments arranged by size: 2703 1730 1316 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2926 3104 844 Size: 2082 178 3489 Fragments arranged by size: 3489 2082 178 BsrBI CCG'CTC Cuts at: 1394 1823 2275 2461 2920 3636 1394 Size: 429 452 186 459 716 3507 Fragments arranged by size: 3507 716 459 452 429 186 BsrDI GCAATG_nn' Cuts at: 65 1248 2311 4244 4418 65 Size: 1183 1063 1933 174 1396 Fragments arranged by size: 1933 1396 1183 1063 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2095 4393 4580 1700 Size: 110 285 2298 187 2869 Fragments arranged by size: 2869 2298 285 187 110 BsrGI T'GTAC_A Cuts at: 96 2007 96 Size: 1911 3838 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5749 BssSI C'ACGA_G Cuts at: 3809 4821 3809 Size: 1012 4737 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5749 BstBI TT'CG_AA Cuts at: 4553 4553 Size: 5749 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5749 BstXI CCAn_nnnn'nTGG Cuts at: 2360 2557 2360 Size: 197 5552 BstYI r'GATC_y Cuts at: 1972 2056 2880 3079 3446 3831 3848 5300 5386 Size: 84 824 199 367 385 17 1452 86 Cuts at: 5386 5398 5744 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 824 385 367 346 199 86 84 17 12 Bsu36I CC'TnA_GG Cuts at: 2709 3000 2709 Size: 291 5458 BtgI C'CryG_G Cuts at: 513 1065 2336 513 Size: 552 1271 3926 Fragments arranged by size: 3926 1271 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5010 Fragments arranged by size: 5010 683 56 BtsI GCAGTG_nn' Cuts at: 3265 4059 4079 3265 Size: 794 20 4935 Fragments arranged by size: 4935 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5749 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5714 DraI TTT'AAA Cuts at: 3053 3053 Size: 5749 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4756 809 Size: 507 3440 1802 Fragments arranged by size: 3440 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2589 2940 3196 Size: 54 1425 50 275 18 759 351 256 Cuts at: 3196 4085 5714 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 759 351 275 256 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3196 1537 Size: 275 1384 4090 Fragments arranged by size: 4090 1384 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2166 2324 3679 1353 Size: 813 158 1355 3423 Fragments arranged by size: 3423 1355 813 158 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4336 4710 4856 387 Size: 1064 138 2747 374 146 1280 Fragments arranged by size: 2747 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2714 2794 3812 4409 5196 5464 Size: 393 479 603 80 1018 597 787 268 Cuts at: 5464 1239 Size: 1524 Fragments arranged by size: 1524 1018 787 603 597 479 393 268 80 EcoNI CCTnn'n_nnAGG Cuts at: 2795 2795 Size: 5749 EcoO109I rG'GnC_Cy Cuts at: 2229 2341 2578 2671 2678 2229 Size: 112 237 93 7 5300 Fragments arranged by size: 5300 237 112 93 7 EcoRI G'AATT_C Cuts at: 3058 3436 3058 Size: 378 5371 EcoRV GAT'ATC Cuts at: 2838 2838 Size: 5749 FspI TGC'GCA Cuts at: 4255 4255 Size: 5749 HaeII r_GCGC'y Cuts at: 1678 4896 1678 Size: 3218 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2983 3015 4390 4422 4464 Size: 32 135 32 1411 32 1375 32 42 Cuts at: 4464 4496 1373 Size: 32 2626 Fragments arranged by size: 2626 1411 1375 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3093 3339 669 Size: 988 281 1155 246 3079 Fragments arranged by size: 3079 1155 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5749 HpaI GTT'AAC Cuts at: 3339 3339 Size: 5749 KpnI G_GTAC'C Cuts at: 3074 3074 Size: 5749 MfeI C'AATT_G Cuts at: 3348 3348 Size: 5749 MluI A'CGCG_T Cuts at: 5625 5625 Size: 5749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2574 4863 5047 1305 Size: 1269 2289 184 2007 Fragments arranged by size: 2289 2007 1269 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2591 10 Size: 54 1425 343 759 3168 Fragments arranged by size: 3168 1425 759 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2984 3707 4066 4225 518 Size: 648 1818 723 359 159 2042 Fragments arranged by size: 2042 1818 723 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2405 2726 3165 3842 4990 5235 1606 Size: 799 321 439 677 1148 245 2120 Fragments arranged by size: 2120 1148 799 677 439 321 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5197 NdeI CA'TA_TG Cuts at: 387 387 Size: 5749 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5749 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 2072 2965 3107 4652 1132 Size: 97 843 893 142 1545 2229 Fragments arranged by size: 2229 1545 893 843 142 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5749 PciI A'CATG_T Cuts at: 4648 4648 Size: 5749 PflMI CCAn_nnn'nTGG Cuts at: 1108 2674 1108 Size: 1566 4183 PfoI T'CCnGG_A Cuts at: 2505 2556 2595 2505 Size: 51 39 5659 Fragments arranged by size: 5659 51 39 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3818 3850 5331 5363 3818 Size: 32 1481 32 4204 Fragments arranged by size: 4204 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2578 2671 2578 Size: 93 5656 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5749 PsiI TTA'TAA Cuts at: 3319 3319 Size: 5749 PspOMI G'GGCC_C Cuts at: 2677 2677 Size: 5749 PstI C_TGCA'G Cuts at: 830 2937 3101 830 Size: 2107 164 3478 Fragments arranged by size: 3478 2107 164 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5749 PvuII CAG'CTG Cuts at: 2405 2726 2405 Size: 321 5428 SalI G'TCGA_C Cuts at: 1936 3091 1936 Size: 1155 4594 SbfI CC_TGCA'GG Cuts at: 2937 3101 2937 Size: 164 5585 ScaI AGT'ACT Cuts at: 3997 5709 3997 Size: 1712 4037 SexAI A'CCwGG_T Cuts at: 2424 2424 Size: 5749 SfcI C'TryA_G Cuts at: 826 1045 2933 3097 4232 4913 5104 826 Size: 219 1888 164 1135 681 191 1471 Fragments arranged by size: 1888 1471 1135 681 219 191 164 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5749 SmaI CCC'GGG Cuts at: 1872 2216 3076 1872 Size: 344 860 4545 Fragments arranged by size: 4545 860 344 SmlI C'TyrA_G Cuts at: 820 1017 1943 2345 3853 4754 5016 820 Size: 197 926 402 1508 901 262 1553 Fragments arranged by size: 1553 1508 926 901 402 262 197 SnaBI TAC'GTA Cuts at: 493 3540 493 Size: 3047 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5749 SphI G_CATG'C Cuts at: 1132 2965 3107 1132 Size: 1833 142 3774 Fragments arranged by size: 3774 1833 142 SspI AAT'ATT Cuts at: 5 52 3673 5 Size: 47 3621 2081 Fragments arranged by size: 3621 2081 47 StuI AGG'CCT Cuts at: 1517 2064 1517 Size: 547 5202 StyI C'CwwG_G Cuts at: 513 1065 2796 3188 513 Size: 552 1731 392 3074 Fragments arranged by size: 3074 1731 552 392 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3776 3793 3946 4131 4576 325 Size: 1255 2196 17 153 185 445 1498 Fragments arranged by size: 2196 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 3995 5707 Size: 274 80 33 51 1443 30 1988 1712 Cuts at: 5707 96 Size: 138 Fragments arranged by size: 1988 1712 1443 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2002 2034 2372 2404 5709 5741 2002 Size: 32 338 32 3305 32 2010 Fragments arranged by size: 3305 2010 338 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5749 XbaI T'CTAG_A Cuts at: 3085 3085 Size: 5749 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5749 XmaI C'CCGG_G Cuts at: 1870 2214 3074 1870 Size: 344 860 4545 Fragments arranged by size: 4545 860 344 XmnI GAAnn'nnTTC Cuts at: 2525 3878 2525 Size: 1353 4396 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3557 276 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI