Restriction map for pFN21AB5454
 (Circular) (Six-base) MAPSORT of: pFN21AB5454.seq  Check: 8727  from: 1  to: 6247



pFN21AB5454  6247 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:31  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3504    4057     278 
   Size:         53      83     186     936    1968     553    2468

  Fragments arranged by size: 

                2468    1968     936     553     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2897    3590    1937 
   Size:        960     693    4594

  Fragments arranged by size: 

                4594     960     693
 
Acc65I G'GTAC_C

Cuts at:   3568    3568 
   Size:       6247
 
AclI AA'CG_TT

Cuts at:   1563    4374    4747    1563 
   Size:       2811     373    3063

  Fragments arranged by size: 

                3063    2811     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2192    2369    4310    5694    5962    1632 
   Size:        560     177    1941    1384     268    1917

  Fragments arranged by size: 

                1941    1917    1384     560     268     177
 
AfeI AGC'GCT

Cuts at:   2081    2081 
   Size:       6247
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6050
 
AflIII A'CryG_T

Cuts at:   1203    5146    6123    1203 
   Size:       3943     977    1327

  Fragments arranged by size: 

                3943    1327     977
 
AgeI A'CCGG_T

Cuts at:   5078    5078 
   Size:       6247
 
AhdI GACnn_n'nnGTC

Cuts at:   3010    4976    3010 
   Size:       1966    4281
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6247
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2847    3374    1184 
   Size:       1663     527    4057

  Fragments arranged by size: 

                4057    1663     527
 
AlwNI CAG_nnn'CTG

Cuts at:   3067    3990    5562    3067 
   Size:        923    1572    3752

  Fragments arranged by size: 

                3752    1572     923
 
ApaLI G'TGCA_C

Cuts at:   4304    5460    4304 
   Size:       1156    5091
 
ApoI r'AATT_y

Cuts at:   1493    3556    3782    3934    6043    1493 
   Size:       2063     226     152    2109    1697

  Fragments arranged by size: 

                2109    2063    1697     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4801     160 
   Size:        872    3769    1606

  Fragments arranged by size: 

                3769    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6247
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2092    3572    1870 
   Size:         73     149    1480    4545

  Fragments arranged by size: 

                4545    1480     149      73
 
BamHI G'GATC_C

Cuts at:   3577    3944    3577 
   Size:        367    5880
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3568    5023     618 
   Size:        325     217     627    1781    1455    1842

  Fragments arranged by size: 

                1842    1781    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2924    3161    3566    1391 
   Size:       1533     237     405    4072

  Fragments arranged by size: 

                4072    1533     405     237
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    5276
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2176    2210    4438    4472    1903 
   Size:         34     239      34    2228      34    3678

  Fragments arranged by size: 

                3678    2228     239      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4139    5349    4139 
   Size:       1210    5037
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6247
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2567    4858     136 
   Size:        107     122      71     631     605     895    2291    1525

  Fragments arranged by size: 

                2291    1525     895     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   6242    6242 
   Size:       6247
 
BlpI GC'TnA_GC

Cuts at:   2723    3664    2723 
   Size:        941    5306
 
Bme1580I G_kGCm'C

Cuts at:   1790    4308    5464    1790 
   Size:       2518    1156    2573

  Fragments arranged by size: 

                2573    2518    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6247
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3978    4936     442 
   Size:        416      53      83     399      24    2561     958    1753

  Fragments arranged by size: 

                2561    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6247
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2938    3199    2938 
   Size:        261    5986
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3161    3347    4907    1239 
   Size:       1922     186    1560    2579

  Fragments arranged by size: 

                2579    1922    1560     186
 
Bpu10I CC'TnA_GC

Cuts at:   3160    3160 
   Size:       6247
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4372    5237    5535    4372 
   Size:        865     298    5084

  Fragments arranged by size: 

                5084     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3187    4910     882 
   Size:        617    1688    1723    2219

  Fragments arranged by size: 

                2219    1723    1688     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4038     493 
   Size:        711    2834    2702

  Fragments arranged by size: 

                2834    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6241    6241 
   Size:       6247
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3501    4054    4436    5071 
   Size:         53      83     186     936    1968     553     382     635

Cuts at:   5071     275 
   Size:       1451

  Fragments arranged by size: 

                1968    1451     936     635     553     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4680    5078    5352    5499    5910    4680 
   Size:        398     274     147     411    5017

  Fragments arranged by size: 

                5017     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3134    3164    1178 
   Size:         30     165      30    1731      30    4261

  Fragments arranged by size: 

                4261    1731     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2318    3065    1186 
   Size:       1132     747    4368

  Fragments arranged by size: 

                4368    1132     747
 
BseYI C'CCAG_C

Cuts at:   2050    2570    5450    5946    2050 
   Size:        520    2880     496    2351

  Fragments arranged by size: 

                2880    2351     520     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2405    2825    1163 
   Size:        573     669     420    4585

  Fragments arranged by size: 

                4585     669     573     420
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2864    3697    4458    5486    1540 
   Size:        275     186     863     833     761    1028    2301

  Fragments arranged by size: 

                2301    1028     863     833     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   4308    4393    5464    4308 
   Size:         85    1071    5091

  Fragments arranged by size: 

                5091    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3758    3851    3758 
   Size:         93    6154
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2060    3492    2060 
   Size:       1432    4815
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2924    3161    3566    4308    4393    5464    1391 
   Size:        399    1134     237     405     742      85    1071    2174

  Fragments arranged by size: 

                2174    1134    1071     742     405     399     237      85
            
 
BspEI T'CCGG_A

Cuts at:   5910    5910 
   Size:       6247
 
BspHI T'CATG_A

Cuts at:   2688    4136    5866    2688 
   Size:       1448    1730    3069

  Fragments arranged by size: 

                3069    1730    1448
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2880    2909    3602     844 
   Size:       2036      29     693    3489

  Fragments arranged by size: 

                3489    2036     693      29
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2365    2386    4134    1394 
   Size:        429     542      21    1748    3507

  Fragments arranged by size: 

                3507    1748     542     429      21
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2829    4742    4916      65 
   Size:       1183    1581    1913     174    1396

  Fragments arranged by size: 

                1913    1581    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3216    4891    5078    1700 
   Size:        110    1406    1675     187    2869

  Fragments arranged by size: 

                2869    1675    1406     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2489      96 
   Size:       2393    3854
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6247
 
BssSI C'ACGA_G

Cuts at:   4307    5319    4307 
   Size:       1012    5235
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6247
 
BstBI TT'CG_AA

Cuts at:   3223    5051    3223 
   Size:       1828    4419
 
BstEII G'GTnAC_C

Cuts at:   1184    3535    1184 
   Size:       2351    3896
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2011    2011 
   Size:       6247
 
BstYI r'GATC_y

Cuts at:   1972    2014    3577    3944    4329    4346    5798    5884    5896 
   Size:         42    1563     367     385      17    1452      86      12

Cuts at:   5896    6242    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1563    1452     385     367     346      86      42
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2562    2926    2941     513 
   Size:        552     939     558     364      15    3819

  Fragments arranged by size: 

                3819     939     558     552     364      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5508

  Fragments arranged by size: 

                5508     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2773    3763    4557    4577    2773 
   Size:        990     794      20    4443

  Fragments arranged by size: 

                4443     990     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6247
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6212
 
DraI TTT'AAA

Cuts at:   3551    3551 
   Size:       6247
 
DraIII CAC_nnn'GTG

Cuts at:   3088    3088 
   Size:       6247
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5254     809 
   Size:        507    3938    1802

  Fragments arranged by size: 

                3938    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2861    2929    3694 
   Size:         54    1425      50     275      18    1031      68     765

Cuts at:   3694    4583    6212       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425    1031     889     765     275      68      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2861    3694    1537 
   Size:        275    1049     833    4090

  Fragments arranged by size: 

                4090    1049     833     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3024    4177    1353 
   Size:       1671    1153    3423

  Fragments arranged by size: 

                3423    1671    1153
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4834    5208    5354     387 
   Size:       1064     138    3245     374     146    1280

  Fragments arranged by size: 

                3245    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2192    2369    3161    3347    4310    4907    5694 
   Size:        393     560     177     792     186     963     597     787

Cuts at:   5694    5962    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     963     792     787     597     560     393     268
                 186     177
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2615    2615 
   Size:       6247
 
EcoO109I rG'GnC_Cy

Cuts at:   2047    2089    2599    2869    2047 
   Size:         42     510     270    5425

  Fragments arranged by size: 

                5425     510     270      42
 
EcoRI G'AATT_C

Cuts at:   3556    3934    3556 
   Size:        378    5869
 
FspI TGC'GCA

Cuts at:   4753    4753 
   Size:       6247
 
HaeII r_GCGC'y

Cuts at:   1678    2083    5394    1678 
   Size:        405    3311    2531

  Fragments arranged by size: 

                3311    2531     405
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2222    2254    2771    2803    2996 
   Size:         32     135      32     650      32     517      32     193

Cuts at:   2996    3028    4888    4920    4962    4994    1373 
   Size:         32    1860      32      42      32    2626

  Fragments arranged by size: 

                2626    1860     650     517     193     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3591    3837     669 
   Size:        988     281    1653     246    3079

  Fragments arranged by size: 

                3079    1653     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    3131     748 
   Size:       2383    3864
 
HpaI GTT'AAC

Cuts at:   3837    3837 
   Size:       6247
 
KpnI G_GTAC'C

Cuts at:   3572    3572 
   Size:       6247
 
MfeI C'AATT_G

Cuts at:   3846    3846 
   Size:       6247
 
MluI A'CGCG_T

Cuts at:   6123    6123 
   Size:       6247
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2347    2355    5361    5545    1305 
   Size:       1042       8    3006     184    2007

  Fragments arranged by size: 

                3006    2007    1042     184       8
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4425

  Fragments arranged by size: 

                4425    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2847    2931    3356    4205    4564    4723     518 
   Size:        648    1681      84     425     849     359     159    2042

  Fragments arranged by size: 

                2042    1681     849     648     425     359     159      84
            
 
MspA1I CmG'CkG

Cuts at:   1606    2110    3663    4340    5488    5733    1606 
   Size:        504    1553     677    1148     245    2120

  Fragments arranged by size: 

                2120    1553    1148     677     504     245
 
NaeI GCC'GGC

Cuts at:   3218    3218 
   Size:       6247
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2926     513 
   Size:        552     939     922    3834

  Fragments arranged by size: 

                3834     939     922     552
 
NdeI CA'TA_TG

Cuts at:    387    3276     387 
   Size:       2889    3358
 
NgoMIV G'CCGG_C

Cuts at:   3216    3216 
   Size:       6247
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6247
 
NotI GC'GGCC_GC

Cuts at:   3694    3694 
   Size:       6247
 
NspI r_CATG'y

Cuts at:   1132    1229    3605    5150    1132 
   Size:         97    2376    1545    2229

  Fragments arranged by size: 

                2376    2229    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    3067    1348 
   Size:       1719    4528
 
PciI A'CATG_T

Cuts at:   5146    5146 
   Size:       6247
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3067    1108 
   Size:       1959    4288
 
PfoI T'CCnGG_A

Cuts at:   2610    3324    2610 
   Size:        714    5533
 
PmeI GTTT'AAAC

Cuts at:   3551    3551 
   Size:       6247
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2607    2639    4316    4348    5829    5861    2607 
   Size:         32    1677      32    1481      32    2993

  Fragments arranged by size: 

                2993    1677    1481      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2047    2089    2599    2869    2047 
   Size:         42     510     270    5425

  Fragments arranged by size: 

                5425     510     270      42
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6247
 
PsiI TTA'TAA

Cuts at:   2748    3817    2748 
   Size:       1069    5178
 
PstI C_TGCA'G

Cuts at:    830    2281    3525    3599     830 
   Size:       1451    1244      74    3478

  Fragments arranged by size: 

                3478    1451    1244      74
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6247
 
PvuII CAG'CTG

Cuts at:   2110    2110 
   Size:       6247
 
SalI G'TCGA_C

Cuts at:   1936    3589    1936 
   Size:       1653    4594
 
SanDI GG'GwC_CC

Cuts at:   2047    2047 
   Size:       6247
 
SbfI CC_TGCA'GG

Cuts at:   3599    3599 
   Size:       6247
 
ScaI AGT'ACT

Cuts at:   2507    4495    6207    2507 
   Size:       1988    1712    2547

  Fragments arranged by size: 

                2547    1988    1712
 
SexAI A'CCwGG_T

Cuts at:   2520    2520 
   Size:       6247
 
SfcI C'TryA_G

Cuts at:    826    1045    2277    3521    3595    4730    5411    5602     826 
   Size:        219    1232    1244      74    1135     681     191    1471

  Fragments arranged by size: 

                1471    1244    1232    1135     681     219     191      74
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6247
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6247
 
SmaI CCC'GGG

Cuts at:   1872    2094    3574    1872 
   Size:        222    1480    4545

  Fragments arranged by size: 

                4545    1480     222
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4351    5252    5514     820 
   Size:        197     926    2408     901     262    1553

  Fragments arranged by size: 

                2408    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4038     493 
   Size:       3545    2702
 
SpeI A'CTAG_T

Cuts at:    152    2098     152 
   Size:       1946    4301
 
SphI G_CATG'C

Cuts at:   1132    3605    1132 
   Size:       2473    3774
 
SspI AAT'ATT

Cuts at:      5      52    3094    4171       5 
   Size:         47    3042    1077    2081

  Fragments arranged by size: 

                3042    2081    1077      47
 
StuI AGG'CCT

Cuts at:   1517    3146    1517 
   Size:       1629    4618
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2646    2926    3686     513 
   Size:        552     939     642     280     760    3074

  Fragments arranged by size: 

                3074     939     760     642     552     280
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4274    4291    4444    4629    5074     325 
   Size:       1255    2694      17     153     185     445    1498

  Fragments arranged by size: 

                2694    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2489    2505    2988 
   Size:        274      80      33      51    1443     512      16     483

Cuts at:   2988    4493    6205      96 
   Size:       1505    1712     138

  Fragments arranged by size: 

                1712    1505    1443     512     483     274     138      80
                  51      33      16
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2467    2499    6207    6239    2467 
   Size:         32    3708      32    2475

  Fragments arranged by size: 

                3708    2475      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6247
 
XbaI T'CTAG_A

Cuts at:   3583    3583 
   Size:       6247
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3250    3250 
   Size:       6247
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6247
 
XmaI C'CCGG_G

Cuts at:   1870    2092    3572    1870 
   Size:        222    1480    4545

  Fragments arranged by size: 

                4545    1480     222
 
XmnI GAAnn'nnTTC

Cuts at:   2081    3377    4376    2081 
   Size:       1296     999    3952

  Fragments arranged by size: 

                3952    1296     999
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3502    4055     276 
   Size:         53      83     186     936    1968     553    2468

  Fragments arranged by size: 

                2468    1968     936     553     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI     NdeI
   NgoMIV     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI    PvuII
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI    BsiWI  BstZ17I   Bsu36I  EcoICRI    EcoRV     FalI     FseI
    FspAI     KasI     NarI     NruI     NsiI     PacI     PmlI   PspOMI
    PspXI     PsrI    RsrII     SacI    SacII     SapI     SfiI     SfoI
     SrfI     SwaI
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