(Circular) (Six-base) MAPSORT of: pFN21AB5454.seq Check: 8727 from: 1 to: 6247 pFN21AB5454 6247 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3504 4057 278 Size: 53 83 186 936 1968 553 2468 Fragments arranged by size: 2468 1968 936 553 186 83 53 AccI GT'mk_AC Cuts at: 1937 2897 3590 1937 Size: 960 693 4594 Fragments arranged by size: 4594 960 693 Acc65I G'GTAC_C Cuts at: 3568 3568 Size: 6247 AclI AA'CG_TT Cuts at: 1563 4374 4747 1563 Size: 2811 373 3063 Fragments arranged by size: 3063 2811 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2192 2369 4310 5694 5962 1632 Size: 560 177 1941 1384 268 1917 Fragments arranged by size: 1941 1917 1384 560 268 177 AfeI AGC'GCT Cuts at: 2081 2081 Size: 6247 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6050 AflIII A'CryG_T Cuts at: 1203 5146 6123 1203 Size: 3943 977 1327 Fragments arranged by size: 3943 1327 977 AgeI A'CCGG_T Cuts at: 5078 5078 Size: 6247 AhdI GACnn_n'nnGTC Cuts at: 3010 4976 3010 Size: 1966 4281 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6247 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2847 3374 1184 Size: 1663 527 4057 Fragments arranged by size: 4057 1663 527 AlwNI CAG_nnn'CTG Cuts at: 3067 3990 5562 3067 Size: 923 1572 3752 Fragments arranged by size: 3752 1572 923 ApaLI G'TGCA_C Cuts at: 4304 5460 4304 Size: 1156 5091 ApoI r'AATT_y Cuts at: 1493 3556 3782 3934 6043 1493 Size: 2063 226 152 2109 1697 Fragments arranged by size: 2109 2063 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4801 160 Size: 872 3769 1606 Fragments arranged by size: 3769 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 AvaI C'yCGr_G Cuts at: 1870 1943 2092 3572 1870 Size: 73 149 1480 4545 Fragments arranged by size: 4545 1480 149 73 BamHI G'GATC_C Cuts at: 3577 3944 3577 Size: 367 5880 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3568 5023 618 Size: 325 217 627 1781 1455 1842 Fragments arranged by size: 1842 1781 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2924 3161 3566 1391 Size: 1533 237 405 4072 Fragments arranged by size: 4072 1533 405 237 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5276 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2176 2210 4438 4472 1903 Size: 34 239 34 2228 34 3678 Fragments arranged by size: 3678 2228 239 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4139 5349 4139 Size: 1210 5037 BclI T'GATC_A Cuts at: 1548 1548 Size: 6247 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2567 4858 136 Size: 107 122 71 631 605 895 2291 1525 Fragments arranged by size: 2291 1525 895 631 605 122 107 71 BglII A'GATC_T Cuts at: 6242 6242 Size: 6247 BlpI GC'TnA_GC Cuts at: 2723 3664 2723 Size: 941 5306 Bme1580I G_kGCm'C Cuts at: 1790 4308 5464 1790 Size: 2518 1156 2573 Fragments arranged by size: 2573 2518 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6247 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3978 4936 442 Size: 416 53 83 399 24 2561 958 1753 Fragments arranged by size: 2561 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6247 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2938 3199 2938 Size: 261 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3161 3347 4907 1239 Size: 1922 186 1560 2579 Fragments arranged by size: 2579 1922 1560 186 Bpu10I CC'TnA_GC Cuts at: 3160 3160 Size: 6247 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4372 5237 5535 4372 Size: 865 298 5084 Fragments arranged by size: 5084 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3187 4910 882 Size: 617 1688 1723 2219 Fragments arranged by size: 2219 1723 1688 617 BsaAI yAC'GTr Cuts at: 493 1204 4038 493 Size: 711 2834 2702 Fragments arranged by size: 2834 2702 711 BsaBI GATnn'nnATC Cuts at: 6241 6241 Size: 6247 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3501 4054 4436 5071 Size: 53 83 186 936 1968 553 382 635 Cuts at: 5071 275 Size: 1451 Fragments arranged by size: 1968 1451 936 635 553 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4680 5078 5352 5499 5910 4680 Size: 398 274 147 411 5017 Fragments arranged by size: 5017 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3134 3164 1178 Size: 30 165 30 1731 30 4261 Fragments arranged by size: 4261 1731 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2318 3065 1186 Size: 1132 747 4368 Fragments arranged by size: 4368 1132 747 BseYI C'CCAG_C Cuts at: 2050 2570 5450 5946 2050 Size: 520 2880 496 2351 Fragments arranged by size: 2880 2351 520 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2405 2825 1163 Size: 573 669 420 4585 Fragments arranged by size: 4585 669 573 420 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2864 3697 4458 5486 1540 Size: 275 186 863 833 761 1028 2301 Fragments arranged by size: 2301 1028 863 833 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4308 4393 5464 4308 Size: 85 1071 5091 Fragments arranged by size: 5091 1071 85 BsmI GAATG_Cn' Cuts at: 3758 3851 3758 Size: 93 6154 BsmBI CGTCTCn'nnnn_ Cuts at: 2060 3492 2060 Size: 1432 4815 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2924 3161 3566 4308 4393 5464 1391 Size: 399 1134 237 405 742 85 1071 2174 Fragments arranged by size: 2174 1134 1071 742 405 399 237 85 BspEI T'CCGG_A Cuts at: 5910 5910 Size: 6247 BspHI T'CATG_A Cuts at: 2688 4136 5866 2688 Size: 1448 1730 3069 Fragments arranged by size: 3069 1730 1448 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2880 2909 3602 844 Size: 2036 29 693 3489 Fragments arranged by size: 3489 2036 693 29 BsrBI CCG'CTC Cuts at: 1394 1823 2365 2386 4134 1394 Size: 429 542 21 1748 3507 Fragments arranged by size: 3507 1748 542 429 21 BsrDI GCAATG_nn' Cuts at: 65 1248 2829 4742 4916 65 Size: 1183 1581 1913 174 1396 Fragments arranged by size: 1913 1581 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3216 4891 5078 1700 Size: 110 1406 1675 187 2869 Fragments arranged by size: 2869 1675 1406 187 110 BsrGI T'GTAC_A Cuts at: 96 2489 96 Size: 2393 3854 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6247 BssSI C'ACGA_G Cuts at: 4307 5319 4307 Size: 1012 5235 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6247 BstBI TT'CG_AA Cuts at: 3223 5051 3223 Size: 1828 4419 BstEII G'GTnAC_C Cuts at: 1184 3535 1184 Size: 2351 3896 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6247 BstYI r'GATC_y Cuts at: 1972 2014 3577 3944 4329 4346 5798 5884 5896 Size: 42 1563 367 385 17 1452 86 12 Cuts at: 5896 6242 1972 Size: 346 1977 Fragments arranged by size: 1977 1563 1452 385 367 346 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2562 2926 2941 513 Size: 552 939 558 364 15 3819 Fragments arranged by size: 3819 939 558 552 364 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5508 Fragments arranged by size: 5508 683 56 BtsI GCAGTG_nn' Cuts at: 2773 3763 4557 4577 2773 Size: 990 794 20 4443 Fragments arranged by size: 4443 990 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6247 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6212 DraI TTT'AAA Cuts at: 3551 3551 Size: 6247 DraIII CAC_nnn'GTG Cuts at: 3088 3088 Size: 6247 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5254 809 Size: 507 3938 1802 Fragments arranged by size: 3938 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2861 2929 3694 Size: 54 1425 50 275 18 1031 68 765 Cuts at: 3694 4583 6212 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1031 889 765 275 68 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2861 3694 1537 Size: 275 1049 833 4090 Fragments arranged by size: 4090 1049 833 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3024 4177 1353 Size: 1671 1153 3423 Fragments arranged by size: 3423 1671 1153 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4834 5208 5354 387 Size: 1064 138 3245 374 146 1280 Fragments arranged by size: 3245 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2369 3161 3347 4310 4907 5694 Size: 393 560 177 792 186 963 597 787 Cuts at: 5694 5962 1239 Size: 268 1524 Fragments arranged by size: 1524 963 792 787 597 560 393 268 186 177 EcoNI CCTnn'n_nnAGG Cuts at: 2615 2615 Size: 6247 EcoO109I rG'GnC_Cy Cuts at: 2047 2089 2599 2869 2047 Size: 42 510 270 5425 Fragments arranged by size: 5425 510 270 42 EcoRI G'AATT_C Cuts at: 3556 3934 3556 Size: 378 5869 FspI TGC'GCA Cuts at: 4753 4753 Size: 6247 HaeII r_GCGC'y Cuts at: 1678 2083 5394 1678 Size: 405 3311 2531 Fragments arranged by size: 3311 2531 405 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2222 2254 2771 2803 2996 Size: 32 135 32 650 32 517 32 193 Cuts at: 2996 3028 4888 4920 4962 4994 1373 Size: 32 1860 32 42 32 2626 Fragments arranged by size: 2626 1860 650 517 193 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3591 3837 669 Size: 988 281 1653 246 3079 Fragments arranged by size: 3079 1653 988 281 246 HindIII A'AGCT_T Cuts at: 748 3131 748 Size: 2383 3864 HpaI GTT'AAC Cuts at: 3837 3837 Size: 6247 KpnI G_GTAC'C Cuts at: 3572 3572 Size: 6247 MfeI C'AATT_G Cuts at: 3846 3846 Size: 6247 MluI A'CGCG_T Cuts at: 6123 6123 Size: 6247 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2347 2355 5361 5545 1305 Size: 1042 8 3006 184 2007 Fragments arranged by size: 3006 2007 1042 184 8 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4425 Fragments arranged by size: 4425 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2847 2931 3356 4205 4564 4723 518 Size: 648 1681 84 425 849 359 159 2042 Fragments arranged by size: 2042 1681 849 648 425 359 159 84 MspA1I CmG'CkG Cuts at: 1606 2110 3663 4340 5488 5733 1606 Size: 504 1553 677 1148 245 2120 Fragments arranged by size: 2120 1553 1148 677 504 245 NaeI GCC'GGC Cuts at: 3218 3218 Size: 6247 NcoI C'CATG_G Cuts at: 513 1065 2004 2926 513 Size: 552 939 922 3834 Fragments arranged by size: 3834 939 922 552 NdeI CA'TA_TG Cuts at: 387 3276 387 Size: 2889 3358 NgoMIV G'CCGG_C Cuts at: 3216 3216 Size: 6247 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6247 NotI GC'GGCC_GC Cuts at: 3694 3694 Size: 6247 NspI r_CATG'y Cuts at: 1132 1229 3605 5150 1132 Size: 97 2376 1545 2229 Fragments arranged by size: 2376 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3067 1348 Size: 1719 4528 PciI A'CATG_T Cuts at: 5146 5146 Size: 6247 PflMI CCAn_nnn'nTGG Cuts at: 1108 3067 1108 Size: 1959 4288 PfoI T'CCnGG_A Cuts at: 2610 3324 2610 Size: 714 5533 PmeI GTTT'AAAC Cuts at: 3551 3551 Size: 6247 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2607 2639 4316 4348 5829 5861 2607 Size: 32 1677 32 1481 32 2993 Fragments arranged by size: 2993 1677 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2047 2089 2599 2869 2047 Size: 42 510 270 5425 Fragments arranged by size: 5425 510 270 42 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6247 PsiI TTA'TAA Cuts at: 2748 3817 2748 Size: 1069 5178 PstI C_TGCA'G Cuts at: 830 2281 3525 3599 830 Size: 1451 1244 74 3478 Fragments arranged by size: 3478 1451 1244 74 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6247 PvuII CAG'CTG Cuts at: 2110 2110 Size: 6247 SalI G'TCGA_C Cuts at: 1936 3589 1936 Size: 1653 4594 SanDI GG'GwC_CC Cuts at: 2047 2047 Size: 6247 SbfI CC_TGCA'GG Cuts at: 3599 3599 Size: 6247 ScaI AGT'ACT Cuts at: 2507 4495 6207 2507 Size: 1988 1712 2547 Fragments arranged by size: 2547 1988 1712 SexAI A'CCwGG_T Cuts at: 2520 2520 Size: 6247 SfcI C'TryA_G Cuts at: 826 1045 2277 3521 3595 4730 5411 5602 826 Size: 219 1232 1244 74 1135 681 191 1471 Fragments arranged by size: 1471 1244 1232 1135 681 219 191 74 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6247 SmaI CCC'GGG Cuts at: 1872 2094 3574 1872 Size: 222 1480 4545 Fragments arranged by size: 4545 1480 222 SmlI C'TyrA_G Cuts at: 820 1017 1943 4351 5252 5514 820 Size: 197 926 2408 901 262 1553 Fragments arranged by size: 2408 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4038 493 Size: 3545 2702 SpeI A'CTAG_T Cuts at: 152 2098 152 Size: 1946 4301 SphI G_CATG'C Cuts at: 1132 3605 1132 Size: 2473 3774 SspI AAT'ATT Cuts at: 5 52 3094 4171 5 Size: 47 3042 1077 2081 Fragments arranged by size: 3042 2081 1077 47 StuI AGG'CCT Cuts at: 1517 3146 1517 Size: 1629 4618 StyI C'CwwG_G Cuts at: 513 1065 2004 2646 2926 3686 513 Size: 552 939 642 280 760 3074 Fragments arranged by size: 3074 939 760 642 552 280 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4274 4291 4444 4629 5074 325 Size: 1255 2694 17 153 185 445 1498 Fragments arranged by size: 2694 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2489 2505 2988 Size: 274 80 33 51 1443 512 16 483 Cuts at: 2988 4493 6205 96 Size: 1505 1712 138 Fragments arranged by size: 1712 1505 1443 512 483 274 138 80 51 33 16 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2467 2499 6207 6239 2467 Size: 32 3708 32 2475 Fragments arranged by size: 3708 2475 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6247 XbaI T'CTAG_A Cuts at: 3583 3583 Size: 6247 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3250 3250 Size: 6247 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6247 XmaI C'CCGG_G Cuts at: 1870 2092 3572 1870 Size: 222 1480 4545 Fragments arranged by size: 4545 1480 222 XmnI GAAnn'nnTTC Cuts at: 2081 3377 4376 2081 Size: 1296 999 3952 Fragments arranged by size: 3952 1296 999 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3502 4055 276 Size: 53 83 186 936 1968 553 2468 Fragments arranged by size: 2468 1968 936 553 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI