(Circular) (Six-base) MAPSORT of: pFN21AE6204.seq Check: 3276 from: 1 to: 5527 pFN21AE6204 5527 bp With 182 enzymes: SgfI * March 18, 2015 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3337 278 Size: 53 83 186 936 1801 2468 Fragments arranged by size: 2468 1801 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2870 1937 Size: 933 4594 Acc65I G'GTAC_C Cuts at: 2848 2848 Size: 5527 AclI AA'CG_TT Cuts at: 1563 3654 4027 1563 Size: 2091 373 3063 Fragments arranged by size: 3063 2091 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2159 3590 4974 5242 1632 Size: 527 1431 1384 268 1917 Fragments arranged by size: 1917 1431 1384 527 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5330 AflIII A'CryG_T Cuts at: 1203 4426 5403 1203 Size: 3223 977 1327 Fragments arranged by size: 3223 1327 977 AgeI A'CCGG_T Cuts at: 4358 4358 Size: 5527 AhdI GACnn_n'nnGTC Cuts at: 4256 4256 Size: 5527 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5527 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2583 1184 Size: 1399 4128 AlwNI CAG_nnn'CTG Cuts at: 3270 4842 3270 Size: 1572 3955 ApaI G_GGCC'C Cuts at: 2033 2033 Size: 5527 ApaLI G'TGCA_C Cuts at: 3584 4740 3584 Size: 1156 4371 ApoI r'AATT_y Cuts at: 1493 2836 3062 3214 5323 1493 Size: 1343 226 152 2109 1697 Fragments arranged by size: 2109 1697 1343 226 152 AseI AT'TA_AT Cuts at: 160 1032 4081 160 Size: 872 3049 1606 Fragments arranged by size: 3049 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5527 AvaI C'yCGr_G Cuts at: 1870 1943 2026 2852 1870 Size: 73 83 826 4545 Fragments arranged by size: 4545 826 83 73 BamHI G'GATC_C Cuts at: 2857 3224 2857 Size: 367 5160 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2104 2257 2848 4303 618 Size: 325 217 627 317 153 591 1455 1842 Fragments arranged by size: 1842 1455 627 591 325 317 217 153 BanII G_rGCy'C Cuts at: 1391 2033 2846 1391 Size: 642 813 4072 Fragments arranged by size: 4072 813 642 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2301 928 Size: 971 402 4154 Fragments arranged by size: 4154 971 402 BbvCI CC'TCA_GC Cuts at: 2153 2153 Size: 5527 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2007 2041 3718 3752 1903 Size: 34 70 34 1677 34 3678 Fragments arranged by size: 3678 1677 70 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3419 4629 3419 Size: 1210 4317 BclI T'GATC_A Cuts at: 1548 1548 Size: 5527 BfrBI ATG'CAT Cuts at: 2738 2738 Size: 5527 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4138 136 Size: 107 122 71 631 605 2466 1525 Fragments arranged by size: 2466 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5522 5522 Size: 5527 BlpI GC'TnA_GC Cuts at: 2168 2944 2168 Size: 776 4751 Bme1580I G_kGCm'C Cuts at: 1790 2033 2109 3588 4744 1790 Size: 243 76 1479 1156 2573 Fragments arranged by size: 2573 1479 1156 243 76 BmgBI CAC'GTC Cuts at: 1318 2058 1318 Size: 740 4787 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2401 3258 4216 Size: 416 53 83 399 24 984 857 958 Cuts at: 4216 442 Size: 1753 Fragments arranged by size: 1753 984 958 857 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5527 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2379 4187 1239 Size: 1140 1808 2579 Fragments arranged by size: 2579 1808 1140 Bpu10I CC'TnA_GC Cuts at: 2153 2153 Size: 5527 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3652 4517 4815 3652 Size: 865 298 4364 Fragments arranged by size: 4364 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4190 882 Size: 617 2691 2219 Fragments arranged by size: 2691 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2790 3318 493 Size: 711 1586 528 2702 Fragments arranged by size: 2702 1586 711 528 BsaBI GATnn'nnATC Cuts at: 2610 5521 2610 Size: 2911 2616 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3334 3716 4351 275 Size: 53 83 186 936 1801 382 635 1451 Fragments arranged by size: 1801 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3960 4358 4632 4779 5190 3960 Size: 398 274 147 411 4297 Fragments arranged by size: 4297 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5302 Fragments arranged by size: 5302 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2757 1186 Size: 1571 3956 BseYI C'CCAG_C Cuts at: 4730 5226 4730 Size: 496 5031 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2114 1163 Size: 573 378 4576 Fragments arranged by size: 4576 573 378 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2977 3738 4766 1540 Size: 275 186 976 761 1028 2301 Fragments arranged by size: 2301 1028 976 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3588 3673 4744 3588 Size: 85 1071 4371 Fragments arranged by size: 4371 1071 85 BsmI GAATG_Cn' Cuts at: 2401 3038 3131 2401 Size: 637 93 4797 Fragments arranged by size: 4797 637 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2109 2846 3588 3673 4744 1391 Size: 399 243 76 737 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 737 399 243 85 76 BspEI T'CCGG_A Cuts at: 5190 5190 Size: 5527 BspHI T'CATG_A Cuts at: 3416 5146 3416 Size: 1730 3797 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2882 844 Size: 2038 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2025 2172 3414 1394 Size: 429 202 147 1242 3507 Fragments arranged by size: 3507 1242 429 202 147 BsrDI GCAATG_nn' Cuts at: 65 1248 2676 4022 4196 65 Size: 1183 1428 1346 174 1396 Fragments arranged by size: 1428 1396 1346 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2187 4171 4358 1700 Size: 110 377 1984 187 2869 Fragments arranged by size: 2869 1984 377 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5527 BssHII G'CGCG_C Cuts at: 1925 2076 1925 Size: 151 5376 BssSI C'ACGA_G Cuts at: 3587 4599 3587 Size: 1012 4515 BstAPI GCAn_nnn'nTGC Cuts at: 794 2387 794 Size: 1593 3934 BstBI TT'CG_AA Cuts at: 4331 4331 Size: 5527 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5527 BstYI r'GATC_y Cuts at: 1972 2857 3224 3609 3626 5078 5164 5176 5522 Size: 885 367 385 17 1452 86 12 346 Cuts at: 5522 1972 Size: 1977 Fragments arranged by size: 1977 1452 885 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2062 2100 2205 2719 513 Size: 552 997 38 105 514 3321 Fragments arranged by size: 3321 997 552 514 105 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4788 Fragments arranged by size: 4788 683 56 BtsI GCAGTG_nn' Cuts at: 3043 3837 3857 3043 Size: 794 20 4713 Fragments arranged by size: 4713 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5527 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5492 DraI TTT'AAA Cuts at: 2831 2831 Size: 5527 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4534 809 Size: 507 3218 1802 Fragments arranged by size: 3218 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2173 2974 Size: 54 1425 50 275 18 222 121 801 Cuts at: 2974 3863 5492 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 801 275 222 121 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2974 1537 Size: 275 1162 4090 Fragments arranged by size: 4090 1162 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3457 1353 Size: 2104 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4114 4488 4634 387 Size: 1064 138 2525 374 146 1280 Fragments arranged by size: 2525 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2379 3590 4187 4974 5242 1239 Size: 393 527 220 1211 597 787 268 1524 Fragments arranged by size: 1524 1211 787 597 527 393 268 220 EcoO109I rG'GnC_Cy Cuts at: 2593 2759 2593 Size: 166 5361 EcoRI G'AATT_C Cuts at: 2836 3214 2836 Size: 378 5149 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2164 2164 Size: 5527 FspI TGC'GCA Cuts at: 4033 4033 Size: 5527 HaeII r_GCGC'y Cuts at: 1678 4674 1678 Size: 2996 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4168 4200 4242 4274 1373 Size: 32 135 32 2596 32 42 32 2626 Fragments arranged by size: 2626 2596 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2871 3117 669 Size: 988 281 933 246 3079 Fragments arranged by size: 3079 988 933 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5527 HpaI GTT'AAC Cuts at: 3117 3117 Size: 5527 KpnI G_GTAC'C Cuts at: 2852 2852 Size: 5527 MfeI C'AATT_G Cuts at: 3126 3126 Size: 5527 MluI A'CGCG_T Cuts at: 5403 5403 Size: 5527 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2112 4641 4825 1305 Size: 807 2529 184 2007 Fragments arranged by size: 2529 2007 807 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3705 Fragments arranged by size: 3705 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 2733 3485 3844 4003 518 Size: 648 1089 478 752 359 159 2042 Fragments arranged by size: 2042 1089 752 648 478 359 159 MspA1I CmG'CkG Cuts at: 1606 2064 2943 3620 4768 5013 1606 Size: 458 879 677 1148 245 2120 Fragments arranged by size: 2120 1148 879 677 458 245 NaeI GCC'GGC Cuts at: 2189 2189 Size: 5527 NcoI C'CATG_G Cuts at: 513 1065 2719 513 Size: 552 1654 3321 Fragments arranged by size: 3321 1654 552 NdeI CA'TA_TG Cuts at: 387 2383 387 Size: 1996 3531 NgoMIV G'CCGG_C Cuts at: 2187 2187 Size: 5527 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5527 NotI GC'GGCC_GC Cuts at: 2974 2974 Size: 5527 NsiI A_TGCA'T Cuts at: 2740 2740 Size: 5527 NspI r_CATG'y Cuts at: 1132 1229 2885 4430 1132 Size: 97 1656 1545 2229 Fragments arranged by size: 2229 1656 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5527 PciI A'CATG_T Cuts at: 4426 4426 Size: 5527 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5527 PmeI GTTT'AAAC Cuts at: 2831 2831 Size: 5527 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3596 3628 5109 5141 3596 Size: 32 1481 32 3982 Fragments arranged by size: 3982 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2593 2759 2593 Size: 166 5361 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5527 PsiI TTA'TAA Cuts at: 3097 3097 Size: 5527 PspOMI G'GGCC_C Cuts at: 2029 2029 Size: 5527 PstI C_TGCA'G Cuts at: 830 2572 2879 830 Size: 1742 307 3478 Fragments arranged by size: 3478 1742 307 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5527 SacII CC_GC'GG Cuts at: 2065 2065 Size: 5527 SalI G'TCGA_C Cuts at: 1936 2869 1936 Size: 933 4594 SanDI GG'GwC_CC Cuts at: 2593 2593 Size: 5527 SbfI CC_TGCA'GG Cuts at: 2879 2879 Size: 5527 ScaI AGT'ACT Cuts at: 3775 5487 3775 Size: 1712 3815 SfcI C'TryA_G Cuts at: 826 1045 2568 2875 4010 4691 4882 826 Size: 219 1523 307 1135 681 191 1471 Fragments arranged by size: 1523 1471 1135 681 307 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5527 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5527 SmaI CCC'GGG Cuts at: 1872 2854 1872 Size: 982 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3631 4532 4794 820 Size: 197 926 1688 901 262 1553 Fragments arranged by size: 1688 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3318 493 Size: 2825 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5527 SphI G_CATG'C Cuts at: 1132 2885 1132 Size: 1753 3774 SspI AAT'ATT Cuts at: 5 52 3451 5 Size: 47 3399 2081 Fragments arranged by size: 3399 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5527 StyI C'CwwG_G Cuts at: 513 1065 2088 2719 2966 513 Size: 552 1023 631 247 3074 Fragments arranged by size: 3074 1023 631 552 247 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2363 2420 3554 3571 3724 3909 4354 Size: 1255 783 57 1134 17 153 185 445 Cuts at: 4354 325 Size: 1498 Fragments arranged by size: 1498 1255 1134 783 445 185 153 57 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3773 5485 96 Size: 274 80 33 51 1443 1796 1712 138 Fragments arranged by size: 1796 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5487 5519 5487 Size: 32 5495 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5527 XbaI T'CTAG_A Cuts at: 2608 2863 2608 Size: 255 5272 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2809 2809 Size: 5527 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5527 XmaI C'CCGG_G Cuts at: 1870 2852 1870 Size: 982 4545 XmnI GAAnn'nnTTC Cuts at: 2339 3656 2339 Size: 1317 4210 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3335 276 Size: 53 83 186 936 1801 2468 Fragments arranged by size: 2468 1801 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI