################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI BspDI BspEI BspMI BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraII DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV FseI FspI HapII Hin1I HindIII HpaII HphI KasI KpnI MfeI MflI MluI MluNI MroI MscI MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AflIII 411 ACATGT A^C[GA][CT]GT AocI 484 CCTGAGG CC^T[ACGT]AGG AvaII 805 GGACC G^G[AT]CC BcgI 1449 GCATAATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 528 GGATAC GTATCC(6/5) BclI 431 TGATCA T^GATCA BsaAI 185 TACGTG [CT]AC^GT[GA] BsaBI 1174 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 281 GAGACC GGTCTC(1/5) BsmBI 589 CGTCTC CGTCTC(1/5) BsmFI 744 GTCCC GGGAC(10/14) Bsp1407I 388 TGTACA T^GTACA BspHI 1256 TCATGA T^CATGA BspLU11I 411 ACATGT A^CATGT BsrBI 818 GAGCGG CCGCTC(-3/-3) BsrGI 388 TGTACA T^GTACA BssSI 1478 CTCGTG CACGAG(-5/-1) Bsu36I 484 CCTGAGG CC^T[ACGT]AGG Cfr13I 805 GGACC G^G[ACGT]CC DraI 718 TTTAAA TTT^AAA DrdI 541 GACAACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 484 CCTGAGG CC^T[ACGT]AGG EcoT14I 926 CCATGG C^C[AT][AT]GG EcoT22I 728 ATGCAT ATGCA^T FauI 42 GCGGG CCCGC(4/6) FbaI 431 TGATCA T^GATCA HaeIII 482 GGCC GG^CC HgaI 55 GACGC GACGC(5/10) HincII 520 GTTAAC GT[CT]^[GA]AC HindII 520 GTTAAC GT[CT]^[GA]AC HpaI 520 GTTAAC GTT^AAC KspI 40 CCGCGG CCGC^GG MamI 1174 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 926 CCATGG C^CATGG NsiI 728 ATGCAT ATGCA^T NspI 411 ACATGT [GA]CATG^[CT] PacI 628 TTAATTAA TTAAT^TAA PstI 460 CTGCAG CTGCA^G RcaI 1256 TCATGA T^CATGA SacI 1283 GAGCTC GAGCT^C SacII 40 CCGCGG CCGC^GG SapI 509 GAAGAGC GCTCTTC(1/4) Sau96I 805 GGACC G^G[ACGT]CC SfcI 460 CTGCAG C^T[GA][CT]AG SpeI 571 ACTAGT A^CTAGT SspBI 388 TGTACA T^GTACA StyI 926 CCATGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 41 CGCG CG^CG 57 CGCG AlwI 1300 GATCC GGATC(4/5) 1483 GATCC AspHI 1104 GAGCAC G[AT]GC[AT]^C 1283 GAGCTC BanII 1283 GAGCTC G[GA]GC[CT]^C 1385 GAGCCC BfaI 572 CTAG C^TAG 1036 CTAG BpmI 592 CTCCAG CTGGAG(16/14) 1073 CTGGAG BsaJI 40 CCGCGG C^C[ACGT][ACGT]GG 926 CCATGG BsiHKAI 1104 GAGCAC G[AT]GC[AT]^C 1283 GAGCTC BsiYI 459 CCTGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1393 CCTCATGCTGG BslI 459 CCTGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1393 CCTCATGCTGG BstF5I 342 GGATG GGATG(2/0) 468 GGATG BstUI 41 CGCG CG^CG 57 CGCG CfoI 8 GCGC GCG^C 14 GCGC DsaI 40 CCGCGG C^C[GA][CT]GG 926 CCATGG FokI 342 GGATG GGATG(9/13) 468 GGATG HaeII 7 AGCGCC [GA]GCGC^[CT] 13 AGCGCC HhaI 8 GCGC GCG^C 14 GCGC HinP1I 8 GCGC G^CGC 14 GCGC MaeI 572 CTAG C^TAG 1036 CTAG MaeIII 408 GTTAC ^GT[ACGT]AC 1426 GTTAC MslI 870 CATTGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1396 CATGCTGGTG MvnI 41 CGCG CG^CG 57 CGCG PvuII 463 CAGCTG CAG^CTG 674 CAGCTG SfaNI 472 GATGC GCATC(5/9) 602 GCATC TaqI 126 TCGA T^CGA 1079 TCGA TfiI 369 GAATC G^A[AT]TC 1369 GATTC TthHB8I 126 TCGA T^CGA 1079 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI Asp700I BbsI BbvI BmyI BpuAI BseRI BsmAI Bsp1286I BsrI BssKI BstNI Cac8I DdeI DpnII EarI EcoRII Fnu4HI HinfI ItaI Ksp632I MaeII MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI ScrFI SspI Tru9I TseI Tsp509I TspRI XmnI