################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## Acc65I AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiEI BsiWI BsmBI Bsp1407I BspEI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tru9I Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 145 GACGTC GACGT^C AccI 696 GTCTAC GT^[AC][GT]AC AcyI 145 GACGTC G[GA]^CG[CT]C AfaI 1302 GTAC GT^AC AflIII 1235 ACATGT A^C[GA][CT]GT AlwNI 434 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1053 GGGCCC GGGCC^C AspHI 172 GAGCAC G[AT]GC[AT]^C BamHI 1016 GGATCC G^GATCC BanI 1339 GGCACC G^G[CT][GA]CC BbsI 858 GAAGAC GAAGAC(2/6) BciVI 480 GTATCC GTATCC(6/5) BfaI 394 CTAG C^TAG BpmI 1213 CTGGAG CTGGAG(16/14) BpuAI 858 GAAGAC GAAGAC(2/6) BsaHI 145 GACGTC G[GA]^CG[CT]C BsaI 926 GAGACC GGTCTC(1/5) BsaWI 632 ACCGGA [AT]^CCGG[AT] BsiHKAI 172 GAGCAC G[AT]GC[AT]^C BsmFI 430 GGGAC GGGAC(10/14) BsmI 591 GAATGC GAATGC(1/-1) BspDI 1294 ATCGAT AT^CGAT BspLU11I 1235 ACATGT A^CATGT BspMI 1097 ACCTGC ACCTGC(4/8) BsrDI 420 CATTGC GCAATG(2/0) BsrFI 811 ACCGGC [GA]^CCGG[CT] BstXI 863 CCACGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 811 ACCGGC [GA]^CCGG[CT] ClaI 1294 ATCGAT AT^CGAT EarI 1159 CTCTTC CTCTTC(1/4) HgaI 585 GACGC GACGC(5/10) Hin1I 145 GACGTC G[GA]^CG[CT]C HinfI 320 GATTC G^A[ACGT]TC Ksp632I 1159 CTCTTC CTCTTC(1/4) MaeI 394 CTAG C^TAG MslI 1065 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 1235 ACATGT [GA]CATG^[CT] PflMI 837 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 861 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 892 CTGCAG CTGCA^G RsaI 1302 GTAC GT^AC SapI 1158 GCTCTTC GCTCTTC(1/4) SmlI 574 CTCAAG C^T[CT][GA]AG StuI 1205 AGGCCT AGG^CCT TfiI 320 GATTC G^A[AT]TC Tsp509I 559 AATT ^AATT Van91I 837 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 393 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### BanII 1053 GGGCCC G[GA]GC[CT]^C 1123 GGGCTC BglI 32 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 95 GCCCCGAGGGC BsmAI 926 GAGAC GTCTC(1/5) 1089 GTCTC BstYI 338 AGATCC [GA]^GATC[CT] 1016 GGATCC CfoI 77 GCGC GCG^C 729 GCGC DraII 1052 GGGGCCC [GA]G^G[ACGT]CC[CT] 1053 GGGCCCC EcoO109I 1052 GGGGCCC [GA]G^G[ACGT]CC[CT] 1053 GGGCCCC HhaI 77 GCGC GCG^C 729 GCGC HinP1I 77 GCGC G^CGC 729 GCGC HphI 216 GGTGA GGTGA(8/7) 17 TCACC MaeII 146 ACGT A^CGT 905 ACGT MaeIII 386 GTGAC ^GT[ACGT]AC 413 GTCAC MboII 858 GAAGA GAAGA(8/7) 1160 TCTTC MflI 338 AGATCC [GA]^GATC[CT] 1016 GGATCC PvuII 70 CAGCTG CAG^CTG 688 CAGCTG SfcI 22 CTACAG C^T[GA][CT]AG 892 CTGCAG SmaI 185 CCCGGG CCC^GGG 212 CCCGGG Tsp45I 386 GTGAC ^GT[GC]AC 413 GTCAC XcmI 899 CCAAGAACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1061 CCAGCATCCAGGTGG XhoII 338 AGATCC [GA]^GATC[CT] 1016 GGATCC XmaCI 185 CCCGGG C^CCGGG 212 CCCGGG XmaI 185 CCCGGG C^CCGGG 212 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AluI AlwI AvaI AvaII BalI BbvI BcnI BmyI BsaJI BseRI BsgI BsiYI BslI BsoBI Bsp1286I BspHI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HpaII ItaI MboI MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RcaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I