(Circular) (Six-base) MAPSORT of: pFN21AE4432.seq Check: 4837 from: 1 to: 6058 pFN21AE4432 6058 bp With 182 enzymes: SgfI * March 28, 2012 14:01 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2154 3868 278 Size: 53 83 186 936 618 1714 2468 Fragments arranged by size: 2468 1714 936 618 186 83 53 AccI GT'mk_AC Cuts at: 1937 2702 3401 1937 Size: 765 699 4594 Fragments arranged by size: 4594 765 699 Acc65I G'GTAC_C Cuts at: 3379 3379 Size: 6058 AclI AA'CG_TT Cuts at: 1563 4185 4558 1563 Size: 2622 373 3063 Fragments arranged by size: 3063 2622 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4121 5505 5773 1632 Size: 2489 1384 268 1917 Fragments arranged by size: 2489 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5861 AflIII A'CryG_T Cuts at: 1203 3240 4957 5934 1203 Size: 2037 1717 977 1327 Fragments arranged by size: 2037 1717 1327 977 AgeI A'CCGG_T Cuts at: 4889 4889 Size: 6058 AhdI GACnn_n'nnGTC Cuts at: 4787 4787 Size: 6058 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6058 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6058 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3227 3259 3227 Size: 32 6026 AlwNI CAG_nnn'CTG Cuts at: 2444 3801 5373 2444 Size: 1357 1572 3129 Fragments arranged by size: 3129 1572 1357 ApaI G_GGCC'C Cuts at: 3062 3062 Size: 6058 ApaLI G'TGCA_C Cuts at: 4115 5271 4115 Size: 1156 4902 ApoI r'AATT_y Cuts at: 1493 3367 3593 3745 5854 1493 Size: 1874 226 152 2109 1697 Fragments arranged by size: 2109 1874 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4612 160 Size: 872 3580 1606 Fragments arranged by size: 3580 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 AvaI C'yCGr_G Cuts at: 1870 1943 2102 2190 2217 3383 1870 Size: 73 159 88 27 1166 4545 Fragments arranged by size: 4545 1166 159 88 73 27 BamHI G'GATC_C Cuts at: 3021 3388 3755 3021 Size: 367 367 5324 Fragments arranged by size: 5324 367 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3344 3379 4834 618 Size: 325 217 627 1557 35 1455 1842 Fragments arranged by size: 1842 1557 1455 627 325 217 35 BanII G_rGCy'C Cuts at: 1391 3062 3132 3377 1391 Size: 1671 70 245 4072 Fragments arranged by size: 4072 1671 245 70 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2870 928 Size: 971 971 4116 Fragments arranged by size: 4116 971 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4249 4283 1903 Size: 34 50 34 2228 34 3678 Fragments arranged by size: 3678 2228 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2496 3950 5160 2496 Size: 1454 1210 3394 Fragments arranged by size: 3394 1454 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6058 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2043 2106 4669 Size: 107 122 71 631 605 371 63 2563 Cuts at: 4669 136 Size: 1525 Fragments arranged by size: 2563 1525 631 605 371 122 107 71 63 BglII A'GATC_T Cuts at: 6053 6053 Size: 6058 BlpI GC'TnA_GC Cuts at: 3475 3475 Size: 6058 Bme1580I G_kGCm'C Cuts at: 1790 2138 3062 3347 4119 5275 1790 Size: 348 924 285 772 1156 2573 Fragments arranged by size: 2573 1156 924 772 348 285 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6058 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2323 2459 3789 Size: 416 53 83 399 24 906 136 1330 Cuts at: 3789 4747 442 Size: 958 1753 Fragments arranged by size: 1753 1330 958 906 416 399 136 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6058 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3111 3111 Size: 6058 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3239 4718 1239 Size: 2000 1479 2579 Fragments arranged by size: 2579 2000 1479 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2564 4183 5048 5346 2564 Size: 1619 865 298 3276 Fragments arranged by size: 3276 1619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2925 4721 882 Size: 617 1426 1796 2219 Fragments arranged by size: 2219 1796 1426 617 BsaAI yAC'GTr Cuts at: 493 1204 3849 493 Size: 711 2645 2702 Fragments arranged by size: 2702 2645 711 BsaBI GATnn'nnATC Cuts at: 6052 6052 Size: 6058 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2151 3865 4247 4882 Size: 53 83 186 936 618 1714 382 635 Cuts at: 4882 275 Size: 1451 Fragments arranged by size: 1714 1451 936 635 618 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2637 4491 4889 5163 5310 5721 2637 Size: 1854 398 274 147 411 2974 Fragments arranged by size: 2974 1854 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3227 3257 3320 3350 1178 Size: 30 165 30 1824 30 63 30 3886 Fragments arranged by size: 3886 1824 165 63 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2310 2313 2561 3148 1186 Size: 946 178 3 248 587 4096 Fragments arranged by size: 4096 946 587 248 178 3 BseYI C'CCAG_C Cuts at: 2429 5261 5757 2429 Size: 2832 496 2730 Fragments arranged by size: 2832 2730 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2522 2642 3308 1163 Size: 573 786 120 666 3913 Fragments arranged by size: 3913 786 666 573 120 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3508 4269 5297 1540 Size: 275 186 1507 761 1028 2301 Fragments arranged by size: 2301 1507 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2181 4119 4204 5275 2181 Size: 1938 85 1071 2964 Fragments arranged by size: 2964 1938 1071 85 BsmI GAATG_Cn' Cuts at: 2602 3569 3662 2602 Size: 967 93 4998 Fragments arranged by size: 4998 967 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 2181 3062 3132 3347 3377 4119 Size: 399 348 43 881 70 215 30 742 Cuts at: 4119 4204 5275 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 881 742 399 348 215 85 70 43 30 BspEI T'CCGG_A Cuts at: 5721 5721 Size: 6058 BspHI T'CATG_A Cuts at: 2811 2883 3132 3947 5677 2811 Size: 72 249 815 1730 3192 Fragments arranged by size: 3192 1730 815 249 72 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3111 3346 3413 844 Size: 2267 235 67 3489 Fragments arranged by size: 3489 2267 235 67 BsrBI CCG'CTC Cuts at: 1394 1823 3945 1394 Size: 429 2122 3507 Fragments arranged by size: 3507 2122 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2424 4553 4727 65 Size: 1183 1176 2129 174 1396 Fragments arranged by size: 2129 1396 1183 1176 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2816 4702 4889 1700 Size: 110 1006 1886 187 2869 Fragments arranged by size: 2869 1886 1006 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6058 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6058 BssSI C'ACGA_G Cuts at: 4118 5130 4118 Size: 1012 5046 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6058 BstBI TT'CG_AA Cuts at: 4862 4862 Size: 6058 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6058 BstXI CCAn_nnnn'nTGG Cuts at: 2875 2875 Size: 6058 BstYI r'GATC_y Cuts at: 1972 2343 3021 3388 3755 4140 4157 5609 5695 Size: 371 678 367 367 385 17 1452 86 Cuts at: 5695 5707 6053 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 678 385 371 367 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2795 2868 3053 513 Size: 552 1730 73 185 3518 Fragments arranged by size: 3518 1730 552 185 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2556 2758 2941 487 Size: 683 56 1330 202 183 3604 Fragments arranged by size: 3604 1330 683 202 183 56 BtsI GCAGTG_nn' Cuts at: 2947 3574 4368 4388 2947 Size: 627 794 20 4617 Fragments arranged by size: 4617 794 627 20 ClaI AT'CG_AT Cuts at: 1554 3300 1554 Size: 1746 4312 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6023 DraI TTT'AAA Cuts at: 3362 3362 Size: 6058 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5065 809 Size: 507 3749 1802 Fragments arranged by size: 3749 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2850 3039 3138 Size: 54 1425 50 275 18 1020 189 99 Cuts at: 3138 3315 3323 3505 4394 6023 8 Size: 177 8 182 889 1629 43 Fragments arranged by size: 1629 1425 1020 889 275 189 182 177 99 54 50 43 18 8 EagI C'GGCC_G Cuts at: 1537 1812 3505 1537 Size: 275 1693 4090 Fragments arranged by size: 4090 1693 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3170 3988 1353 Size: 1817 818 3423 Fragments arranged by size: 3423 1817 818 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4645 5019 5165 387 Size: 1064 138 3056 374 146 1280 Fragments arranged by size: 3056 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3239 4121 4718 5505 5773 1239 Size: 393 1607 882 597 787 268 1524 Fragments arranged by size: 1607 1524 882 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3058 3059 3058 Size: 1 6057 EcoRI G'AATT_C Cuts at: 3367 3745 3367 Size: 378 5680 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2170 3271 2170 Size: 1101 4957 FspI TGC'GCA Cuts at: 4564 4564 Size: 6058 HaeII r_GCGC'y Cuts at: 1678 5205 1678 Size: 3527 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2328 2360 3163 3195 3320 Size: 32 135 32 756 32 803 32 125 Cuts at: 3320 3352 4699 4731 4773 4805 1373 Size: 32 1347 32 42 32 2626 Fragments arranged by size: 2626 1347 803 756 135 125 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3402 3648 669 Size: 988 281 1464 246 3079 Fragments arranged by size: 3079 1464 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6058 HpaI GTT'AAC Cuts at: 3648 3648 Size: 6058 KpnI G_GTAC'C Cuts at: 3383 3383 Size: 6058 MfeI C'AATT_G Cuts at: 3657 3657 Size: 6058 MluI A'CGCG_T Cuts at: 5934 5934 Size: 6058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2265 5172 5356 1305 Size: 960 2907 184 2007 Fragments arranged by size: 2907 2007 960 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2852 3041 3140 10 Size: 54 1425 343 1020 189 99 2928 Fragments arranged by size: 2928 1425 1020 343 189 99 54 MslI CAynn'nnrTG Cuts at: 518 1166 3074 4016 4375 4534 518 Size: 648 1908 942 359 159 2042 Fragments arranged by size: 2042 1908 942 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2077 2322 2695 3126 3474 4151 5299 5544 Size: 471 245 373 431 348 677 1148 245 Cuts at: 5544 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 471 431 373 348 245 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5506 NdeI CA'TA_TG Cuts at: 387 387 Size: 6058 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6058 NotI GC'GGCC_GC Cuts at: 3505 3505 Size: 6058 NspI r_CATG'y Cuts at: 1132 1229 3244 3416 4961 1132 Size: 97 2015 172 1545 2229 Fragments arranged by size: 2229 2015 1545 172 97 PasI CC'CwG_GG Cuts at: 1348 2332 1348 Size: 984 5074 PciI A'CATG_T Cuts at: 3240 4957 3240 Size: 1717 4341 PflMI CCAn_nnn'nTGG Cuts at: 1108 2848 1108 Size: 1740 4318 PfoI T'CCnGG_A Cuts at: 2874 3024 3216 2874 Size: 150 192 5716 Fragments arranged by size: 5716 192 150 PmeI GTTT'AAAC Cuts at: 3362 3362 Size: 6058 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3227 3259 4127 4159 5640 5672 3227 Size: 32 868 32 1481 32 3613 Fragments arranged by size: 3613 1481 868 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2870 1613 Size: 1257 4801 PsiI TTA'TAA Cuts at: 3628 3628 Size: 6058 PspOMI G'GGCC_C Cuts at: 3058 3058 Size: 6058 PstI C_TGCA'G Cuts at: 830 2901 3410 830 Size: 2071 509 3478 Fragments arranged by size: 3478 2071 509 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6058 PvuII CAG'CTG Cuts at: 2077 2695 2077 Size: 618 5440 SalI G'TCGA_C Cuts at: 1936 3400 1936 Size: 1464 4594 SapI GCTCTTCn'nnn_ Cuts at: 3170 3170 Size: 6058 SbfI CC_TGCA'GG Cuts at: 3410 3410 Size: 6058 ScaI AGT'ACT Cuts at: 4306 6018 4306 Size: 1712 4346 SfcI C'TryA_G Cuts at: 826 1045 2027 2897 3406 4541 5222 5413 826 Size: 219 982 870 509 1135 681 191 1471 Fragments arranged by size: 1471 1135 982 870 681 509 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6058 SmaI CCC'GGG Cuts at: 1872 2192 2219 3385 1872 Size: 320 27 1166 4545 Fragments arranged by size: 4545 1166 320 27 SmlI C'TyrA_G Cuts at: 820 1017 1943 2579 4162 5063 5325 820 Size: 197 926 636 1583 901 262 1553 Fragments arranged by size: 1583 1553 926 901 636 262 197 SnaBI TAC'GTA Cuts at: 493 3849 493 Size: 3356 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6058 SphI G_CATG'C Cuts at: 1132 3416 1132 Size: 2284 3774 SspI AAT'ATT Cuts at: 5 52 3982 5 Size: 47 3930 2081 Fragments arranged by size: 3930 2081 47 StuI AGG'CCT Cuts at: 1517 3212 1517 Size: 1695 4363 StyI C'CwwG_G Cuts at: 513 1065 3497 513 Size: 552 2432 3074 Fragments arranged by size: 3074 2432 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3011 3362 4085 4102 4255 4440 4885 Size: 1255 1431 351 723 17 153 185 445 Cuts at: 4885 325 Size: 1498 Fragments arranged by size: 1498 1431 1255 723 445 351 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4304 6016 96 Size: 274 80 33 51 1443 2327 1712 138 Fragments arranged by size: 2327 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6018 6050 6018 Size: 32 6026 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6058 XbaI T'CTAG_A Cuts at: 2398 3394 2398 Size: 996 5062 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2911 3073 2911 Size: 162 5896 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6058 XmaI C'CCGG_G Cuts at: 1870 2190 2217 3383 1870 Size: 320 27 1166 4545 Fragments arranged by size: 4545 1166 320 27 XmnI GAAnn'nnTTC Cuts at: 4187 4187 Size: 6058 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2152 3866 276 Size: 53 83 186 936 618 1714 2468 Fragments arranged by size: 2468 1714 936 618 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI