################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI AocI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbeI BbrPI BciVI BfaI BfrI BlnI BlpI Bpu1102I BsaBI BsiWI BsmI BspDI BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eco81I EcoO65I EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MunI NarI NdeI NheI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 944 TCCGGA T^CCGGA AcyI 430 GGCGTC G[GA]^CG[CT]C AhdI 1066 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1182 GTGCAC G^TGCAC Aor51HI 767 AGCGCT AGC^GCT ApaLI 1182 GTGCAC G^TGCAC AspEI 1066 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1645 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 916 GTCTTC GAAGAC(2/6) BbvCI 1745 CCTCAGC CCTCAGC(-5/-2) BcgI 1248 CGAGGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 515 TGATCA T^GATCA BglII 647 AGATCT A^GATCT BpuAI 916 GTCTTC GAAGAC(2/6) BsaHI 430 GGCGTC G[GA]^CG[CT]C BsaI 1471 GAGACC GGTCTC(1/5) BsaWI 944 TCCGGA [AT]^CCGG[AT] BseAI 944 TCCGGA T^CCGGA BsmBI 2263 GAGACG CGTCTC(1/5) BspEI 944 TCCGGA T^CCGGA BspLU11I 2060 ACATGT A^CATGT BsrDI 1190 GCAATG GCAATG(2/0) BssSI 1246 CACGAG CACGAG(-5/-1) BstAPI 242 GCACCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 243 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1066 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 767 AGCGCT AGC^GCT EcoRI 675 GAATTC G^AATTC FbaI 515 TGATCA T^GATCA HaeII 767 AGCGCT [GA]GCGC^[CT] HgaI 431 GCGTC GACGC(5/10) Hin1I 430 GGCGTC G[GA]^CG[CT]C MluI 1853 ACGCGT A^CGCGT MroI 944 TCCGGA T^CCGGA NaeI 1781 GCCGGC GCC^GGC NgoMIV 1781 GCCGGC G^CCGGC NotI 1316 GCGGCCGC GC^GGCCGC PaeR7I 936 CTCGAG C^TCGAG PflFI 1645 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1228 GAGTC GAGTC(4/5) ScaI 104 AGTACT AGT^ACT SmlI 936 CTCGAG C^T[CT][GA]AG SphI 409 GCATGC GCATG^C StuI 7 AGGCCT AGG^CCT Tth111I 1645 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 936 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 499 AAATTT [GA]^AATT[CT] 675 GAATTC AflIII 1853 ACGCGT A^C[GA][CT]GT 2060 ACATGT AlwNI 1124 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG 1962 CAGCTTCTG ApaI 355 GGGCCC GGGCC^C 831 GGGCCC ApoI 499 AAATTT [GA]^AATT[CT] 675 GAATTC BcnI 210 CCGGG CC^[GC]GG 291 CCGGG BsaAI 304 TACGTG [CT]AC^GT[GA] 613 TACGTG Bsp1407I 737 TGTACA T^GTACA 2228 TGTACA BspHI 1514 TCATGA T^CATGA 2186 TCATGA BsrGI 737 TGTACA T^GTACA 2228 TGTACA EarI 189 GAAGAG CTCTTC(1/4) 2260 GAAGAG EcoNI 752 CCTCACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2124 CCTGTTCCAGG HincII 1495 GTCAAC GT[CT]^[GA]AC 2092 GTTGAC HindII 1495 GTCAAC GT[CT]^[GA]AC 2092 GTTGAC Ksp632I 189 GAAGAG CTCTTC(1/4) 2260 GAAGAG MseI 2141 TTAA T^TAA 2299 TTAA NciI 210 CCGGG CC^[GC]GG 291 CCGGG PpuMI 1064 GGGACCT [GA]G^G[AT]CC[CT] 1366 GGGTCCC RcaI 1514 TCATGA T^CATGA 2186 TCATGA SspBI 737 TGTACA T^GTACA 2228 TGTACA Tru9I 2141 TTAA T^TAA 2299 TTAA XcmI 818 CCAGCTGCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1961 CCAGCTTCTGCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaI AvaII BalI BanI BanII BbvI BglI BmyI BpmI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII