(Circular) (Six-base) MAPSORT of: pFN21AE4578.seq Check: 3999 from: 1 to: 7027 pFN21AE4578 7027 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2882 2882 Size: 7027 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4837 278 Size: 53 83 186 936 3301 2468 Fragments arranged by size: 3301 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4370 1937 Size: 2433 4594 Acc65I G'GTAC_C Cuts at: 4348 4348 Size: 7027 AclI AA'CG_TT Cuts at: 1563 5154 5527 1563 Size: 3591 373 3063 Fragments arranged by size: 3591 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2908 4243 4276 5090 6474 6742 1632 Size: 1276 1335 33 814 1384 268 1917 Fragments arranged by size: 1917 1384 1335 1276 814 268 33 AfeI AGC'GCT Cuts at: 2774 2774 Size: 7027 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6830 AflIII A'CryG_T Cuts at: 1203 3858 4065 5926 6903 1203 Size: 2655 207 1861 977 1327 Fragments arranged by size: 2655 1861 1327 977 207 AgeI A'CCGG_T Cuts at: 5858 5858 Size: 7027 AhdI GACnn_n'nnGTC Cuts at: 3076 5756 3076 Size: 2680 4347 AleI CACnn'nnGTG Cuts at: 1166 2603 3944 4109 4248 1166 Size: 1437 1341 165 139 3945 Fragments arranged by size: 3945 1437 1341 165 139 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7027 AlwNI CAG_nnn'CTG Cuts at: 3134 3972 4770 6342 3134 Size: 838 798 1572 3819 Fragments arranged by size: 3819 1572 838 798 ApaI G_GGCC'C Cuts at: 2364 2840 2364 Size: 476 6551 ApaLI G'TGCA_C Cuts at: 3187 5084 6240 3187 Size: 1897 1156 3974 Fragments arranged by size: 3974 1897 1156 ApoI r'AATT_y Cuts at: 1493 2504 2680 4336 4562 4714 6823 1493 Size: 1011 176 1656 226 152 2109 1697 Fragments arranged by size: 2109 1697 1656 1011 226 176 152 AseI AT'TA_AT Cuts at: 160 1032 5581 160 Size: 872 4549 1606 Fragments arranged by size: 4549 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7027 AvaI C'yCGr_G Cuts at: 1870 1943 2105 2941 3538 4352 1870 Size: 73 162 836 597 814 4545 Fragments arranged by size: 4545 836 814 597 162 73 BamHI G'GATC_C Cuts at: 4357 4724 4357 Size: 367 6660 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2246 2996 3938 4348 5803 Size: 325 217 627 459 750 942 410 1455 Cuts at: 5803 618 Size: 1842 Fragments arranged by size: 1842 1455 942 750 627 459 410 325 217 BanII G_rGCy'C Cuts at: 1391 2364 2840 4302 4346 1391 Size: 973 476 1462 44 4072 Fragments arranged by size: 4072 1462 973 476 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2914 928 Size: 971 1015 5041 Fragments arranged by size: 5041 1015 971 BbvCI CC'TCA_GC Cuts at: 3751 3751 Size: 7027 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3242 3276 5218 5252 1903 Size: 34 1305 34 1942 34 3678 Fragments arranged by size: 3678 1942 1305 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4919 6129 4919 Size: 1210 5817 BclI T'GATC_A Cuts at: 1548 2520 1548 Size: 972 6055 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2162 2244 3125 Size: 107 122 71 631 605 490 82 881 Cuts at: 3125 3318 3444 3600 5638 136 Size: 193 126 156 2038 1525 Fragments arranged by size: 2038 1525 881 631 605 490 193 156 126 122 107 82 71 BglII A'GATC_T Cuts at: 2652 7022 2652 Size: 4370 2657 BlpI GC'TnA_GC Cuts at: 4444 4444 Size: 7027 Bme1580I G_kGCm'C Cuts at: 1790 2249 2364 2840 3191 4116 5088 6244 1790 Size: 459 115 476 351 925 972 1156 2573 Fragments arranged by size: 2573 1156 972 925 476 459 351 115 BmgBI CAC'GTC Cuts at: 1318 2287 3586 4111 1318 Size: 969 1299 525 4234 Fragments arranged by size: 4234 1299 969 525 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2597 3155 3415 Size: 416 53 83 399 24 1180 558 260 Cuts at: 3415 4758 5716 442 Size: 1343 958 1753 Fragments arranged by size: 1753 1343 1180 958 558 416 399 260 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7027 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2594 2594 Size: 7027 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2806 3713 4140 5687 1239 Size: 1567 907 427 1547 2579 Fragments arranged by size: 2579 1567 1547 907 427 Bpu10I CC'TnA_GC Cuts at: 2069 3751 2069 Size: 1682 5345 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5152 6017 6315 5152 Size: 865 298 5864 Fragments arranged by size: 5864 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3470 5690 882 Size: 617 1971 2220 2219 Fragments arranged by size: 2220 2219 1971 617 BsaAI yAC'GTr Cuts at: 493 1204 2311 2620 4818 493 Size: 711 1107 309 2198 2702 Fragments arranged by size: 2702 2198 1107 711 309 BsaBI GATnn'nnATC Cuts at: 7021 7021 Size: 7027 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2436 4834 5216 5851 Size: 53 83 186 936 903 2398 382 635 Cuts at: 5851 275 Size: 1451 Fragments arranged by size: 2398 1451 936 903 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2949 5460 5858 6132 6279 6690 2949 Size: 2511 398 274 147 411 3286 Fragments arranged by size: 3286 2511 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2252 2282 2306 2336 3467 Size: 30 165 30 849 30 24 30 1131 Cuts at: 3467 3497 1178 Size: 30 4708 Fragments arranged by size: 4708 1131 849 165 30 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2329 3269 3746 3793 1186 Size: 1143 940 477 47 4420 Fragments arranged by size: 4420 1143 940 477 47 BseYI C'CCAG_C Cuts at: 2186 2302 2430 2833 3103 3442 4059 6230 6726 Size: 116 128 403 270 339 617 2171 496 Cuts at: 6726 2186 Size: 2487 Fragments arranged by size: 2487 2171 617 496 403 339 270 128 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2497 2782 3629 3695 1163 Size: 573 761 285 847 66 4495 Fragments arranged by size: 4495 847 761 573 285 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2895 3325 3853 4477 5238 6266 Size: 275 186 894 430 528 624 761 1028 Cuts at: 6266 1540 Size: 2301 Fragments arranged by size: 2301 1028 894 761 624 528 430 275 186 BsiHKAI G_wGCw'C Cuts at: 2024 3030 3191 3539 4290 5088 5173 6244 2024 Size: 1006 161 348 751 798 85 1071 2807 Fragments arranged by size: 2807 1071 1006 798 751 348 161 85 BsmI GAATG_Cn' Cuts at: 4538 4631 4538 Size: 93 6934 BsmBI CGTCTCn'nnnn_ Cuts at: 4262 4262 Size: 7027 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2249 2364 2840 3030 3191 3539 Size: 399 234 225 115 476 190 161 348 Cuts at: 3539 4116 4290 4302 4346 5088 5173 6244 1391 Size: 577 174 12 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 577 476 399 348 234 225 190 174 161 115 85 44 12 BspEI T'CCGG_A Cuts at: 2949 6690 2949 Size: 3741 3286 BspHI T'CATG_A Cuts at: 3519 4191 4916 6646 3519 Size: 672 725 1730 3900 Fragments arranged by size: 3900 1730 725 672 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2882 2908 3930 4315 4382 844 Size: 2038 26 1022 385 67 3489 Fragments arranged by size: 3489 2038 1022 385 67 26 BsrBI CCG'CTC Cuts at: 1394 1823 4914 1394 Size: 429 3091 3507 Fragments arranged by size: 3507 3091 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3202 5522 5696 65 Size: 1183 1954 2320 174 1396 Fragments arranged by size: 2320 1954 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2122 2801 2890 3699 3786 5671 5858 Size: 110 312 679 89 809 87 1885 187 Cuts at: 5858 1700 Size: 2869 Fragments arranged by size: 2869 1885 809 679 312 187 110 89 87 BsrGI T'GTAC_A Cuts at: 96 2742 4233 96 Size: 2646 1491 2890 Fragments arranged by size: 2890 2646 1491 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7027 BssSI C'ACGA_G Cuts at: 3251 5087 6099 3251 Size: 1836 1012 4179 Fragments arranged by size: 4179 1836 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2253 794 Size: 1459 5568 BstBI TT'CG_AA Cuts at: 5831 5831 Size: 7027 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7027 BstXI CCAn_nnnn'nTGG Cuts at: 2390 3691 3821 4250 2390 Size: 1301 130 429 5167 Fragments arranged by size: 5167 1301 429 130 BstYI r'GATC_y Cuts at: 1972 2652 3180 3810 3864 4357 4724 5109 5126 Size: 680 528 630 54 493 367 385 17 Cuts at: 5126 6578 6664 6676 7022 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 680 630 528 493 385 367 346 86 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2790 2895 3288 3423 3726 3774 Size: 552 939 786 105 393 135 303 48 Cuts at: 3774 4050 513 Size: 276 3490 Fragments arranged by size: 3490 939 786 552 393 303 276 135 105 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2271 487 Size: 683 56 1045 5243 Fragments arranged by size: 5243 1045 683 56 BtsI GCAGTG_nn' Cuts at: 3562 3725 4543 5337 5357 3562 Size: 163 818 794 20 5232 Fragments arranged by size: 5232 818 794 163 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7027 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6992 DraI TTT'AAA Cuts at: 4331 4331 Size: 7027 DraIII CAC_nnn'GTG Cuts at: 2253 2253 Size: 7027 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6034 809 Size: 507 4718 1802 Fragments arranged by size: 4718 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2163 2236 2673 Size: 54 1425 50 275 18 333 73 437 Cuts at: 2673 2780 2892 3081 3322 3397 3823 3850 4216 Size: 107 112 189 241 75 426 27 366 Cuts at: 4216 4474 5363 6992 8 Size: 258 889 1629 43 Fragments arranged by size: 1629 1425 889 437 426 366 333 275 258 241 189 112 107 75 73 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2892 3322 3850 4474 1537 Size: 275 1080 430 528 624 4090 Fragments arranged by size: 4090 1080 624 528 430 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2189 4260 4957 1353 Size: 836 2071 697 3423 Fragments arranged by size: 3423 2071 836 697 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3618 5614 5988 6134 387 Size: 1064 138 2029 1996 374 146 1280 Fragments arranged by size: 2029 1996 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2806 2908 3713 4140 4243 4276 5090 Size: 393 1174 102 805 427 103 33 814 Cuts at: 5090 5687 6474 6742 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1174 814 805 787 597 427 393 268 103 102 33 EcoNI CCTnn'n_nnAGG Cuts at: 2761 4133 2761 Size: 1372 5655 EcoO109I rG'GnC_Cy Cuts at: 2149 2360 2837 3070 3372 2149 Size: 211 477 233 302 5804 Fragments arranged by size: 5804 477 302 233 211 EcoRI G'AATT_C Cuts at: 2680 4336 4714 2680 Size: 1656 378 4993 Fragments arranged by size: 4993 1656 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3985 3985 Size: 7027 FspI TGC'GCA Cuts at: 5533 5533 Size: 7027 HaeII r_GCGC'y Cuts at: 1678 2776 6174 1678 Size: 1098 3398 2531 Fragments arranged by size: 3398 2531 1098 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2252 2284 2306 2338 2924 Size: 32 135 32 680 32 22 32 586 Cuts at: 2924 2956 3062 3094 5668 5700 5742 5774 1373 Size: 32 106 32 2574 32 42 32 2626 Fragments arranged by size: 2626 2574 680 586 135 106 42 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3502 4099 4371 4617 669 Size: 988 281 1564 597 272 246 3079 Fragments arranged by size: 3079 1564 988 597 281 272 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7027 HpaI GTT'AAC Cuts at: 4617 4617 Size: 7027 KpnI G_GTAC'C Cuts at: 4352 4352 Size: 7027 MfeI C'AATT_G Cuts at: 4626 4626 Size: 7027 MluI A'CGCG_T Cuts at: 3858 6903 3858 Size: 3045 3982 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3855 4206 6141 6325 1305 Size: 2550 351 1935 184 2007 Fragments arranged by size: 2550 2007 1935 351 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2165 2238 2782 3399 3825 Size: 54 1425 343 333 73 544 617 426 Cuts at: 3825 10 Size: 3212 Fragments arranged by size: 3212 1425 617 544 426 343 333 73 54 MslI CAynn'nnrTG Cuts at: 518 1166 2603 3495 3819 3902 3944 4049 4109 Size: 648 1437 892 324 83 42 105 60 Cuts at: 4109 4229 4248 4985 5344 5503 518 Size: 120 19 737 359 159 2042 Fragments arranged by size: 2042 1437 892 737 648 359 324 159 120 105 83 60 42 19 MspA1I CmG'CkG Cuts at: 1606 2037 2091 2176 2826 3442 4296 4315 4443 Size: 431 54 85 650 616 854 19 128 Cuts at: 4443 5120 6268 6513 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 854 677 650 616 431 245 128 85 54 19 NaeI GCC'GGC Cuts at: 3788 3788 Size: 7027 NcoI C'CATG_G Cuts at: 513 1065 2004 2790 3288 3423 3726 4050 513 Size: 552 939 786 498 135 303 324 3490 Fragments arranged by size: 3490 939 786 552 498 324 303 135 NdeI CA'TA_TG Cuts at: 387 387 Size: 7027 NgoMIV G'CCGG_C Cuts at: 3786 3786 Size: 7027 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7027 NotI GC'GGCC_GC Cuts at: 3322 4474 3322 Size: 1152 5875 NspI r_CATG'y Cuts at: 1132 1229 2418 2704 4069 4385 5930 1132 Size: 97 1189 286 1365 316 1545 2229 Fragments arranged by size: 2229 1545 1365 1189 316 286 97 PasI CC'CwG_GG Cuts at: 1348 2714 3598 1348 Size: 1366 884 4777 Fragments arranged by size: 4777 1366 884 PciI A'CATG_T Cuts at: 4065 5926 4065 Size: 1861 5166 PflMI CCAn_nnn'nTGG Cuts at: 1108 2325 2430 3898 1108 Size: 1217 105 1468 4237 Fragments arranged by size: 4237 1468 1217 105 PfoI T'CCnGG_A Cuts at: 2324 3471 2324 Size: 1147 5880 PmeI GTTT'AAAC Cuts at: 4331 4331 Size: 7027 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3728 3760 5096 5128 6609 6641 3728 Size: 32 1336 32 1481 32 4114 Fragments arranged by size: 4114 1481 1336 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3070 3372 3070 Size: 302 6725 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7027 PsiI TTA'TAA Cuts at: 4597 4597 Size: 7027 PspOMI G'GGCC_C Cuts at: 2360 2836 2360 Size: 476 6551 PstI C_TGCA'G Cuts at: 830 2036 3098 3720 4379 830 Size: 1206 1062 622 659 3478 Fragments arranged by size: 3478 1206 1062 659 622 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7027 PvuII CAG'CTG Cuts at: 2037 2091 2176 2826 3442 4315 2037 Size: 54 85 650 616 873 4749 Fragments arranged by size: 4749 873 650 616 85 54 SalI G'TCGA_C Cuts at: 1936 4369 1936 Size: 2433 4594 SanDI GG'GwC_CC Cuts at: 3372 3372 Size: 7027 SbfI CC_TGCA'GG Cuts at: 4379 4379 Size: 7027 ScaI AGT'ACT Cuts at: 2111 5275 6987 2111 Size: 3164 1712 2151 Fragments arranged by size: 3164 2151 1712 SexAI A'CCwGG_T Cuts at: 3072 3479 3651 3072 Size: 407 172 6448 Fragments arranged by size: 6448 407 172 SfcI C'TryA_G Cuts at: 826 1045 2032 3094 3716 4375 5510 6191 6382 Size: 219 987 1062 622 659 1135 681 191 Cuts at: 6382 826 Size: 1471 Fragments arranged by size: 1471 1135 1062 987 681 659 622 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7027 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7027 SmaI CCC'GGG Cuts at: 1872 4354 1872 Size: 2482 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2941 5131 6032 6294 820 Size: 197 926 998 2190 901 262 1553 Fragments arranged by size: 2190 1553 998 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4818 493 Size: 4325 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7027 SphI G_CATG'C Cuts at: 1132 2418 4385 1132 Size: 1286 1967 3774 Fragments arranged by size: 3774 1967 1286 SspI AAT'ATT Cuts at: 5 52 4951 5 Size: 47 4899 2081 Fragments arranged by size: 4899 2081 47 StuI AGG'CCT Cuts at: 1517 2014 1517 Size: 497 6530 StyI C'CwwG_G Cuts at: 513 1065 2004 2790 3288 3423 3726 3942 4050 Size: 552 939 786 498 135 303 216 108 Cuts at: 4050 4466 513 Size: 416 3074 Fragments arranged by size: 3074 939 786 552 498 416 303 216 135 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3779 3926 5054 5071 5224 5409 5854 Size: 1255 2199 147 1128 17 153 185 445 Cuts at: 5854 325 Size: 1498 Fragments arranged by size: 2199 1498 1255 1128 445 185 153 147 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2109 2226 2387 Size: 274 80 33 51 1443 132 117 161 Cuts at: 2387 2742 4233 5273 6985 96 Size: 355 1491 1040 1712 138 Fragments arranged by size: 1712 1491 1443 1040 355 274 161 138 132 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3379 3411 6987 7019 3379 Size: 32 3576 32 3387 Fragments arranged by size: 3576 3387 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3653 1316 Size: 2337 4690 XbaI T'CTAG_A Cuts at: 4363 4363 Size: 7027 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2830 3973 2830 Size: 1143 5884 XhoI C'TCGA_G Cuts at: 1943 2941 1943 Size: 998 6029 XmaI C'CCGG_G Cuts at: 1870 4352 1870 Size: 2482 4545 XmnI GAAnn'nnTTC Cuts at: 5156 5156 Size: 7027 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4835 276 Size: 53 83 186 936 3301 2468 Fragments arranged by size: 3301 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI