(Linear) (Six-base) MAPSORT of: KIEE4578.seq Check: 2305 from: 1 to: 2322 KIEE4578.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 877 2322 Size: 877 1445 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 903 2238 2271 2322 Size: 903 1335 33 51 Fragments arranged by size: 1335 903 51 33 AfeI AGC'GCT Cuts at: 0 769 2322 Size: 769 1553 AflIII A'CryG_T Cuts at: 0 1853 2060 2322 Size: 1853 207 262 Fragments arranged by size: 1853 262 207 AhdI GACnn_n'nnGTC Cuts at: 0 1071 2322 Size: 1071 1251 AleI CACnn'nnGTG Cuts at: 0 598 1939 2104 2243 2322 Size: 598 1341 165 139 79 Fragments arranged by size: 1341 598 165 139 79 AlwNI CAG_nnn'CTG Cuts at: 0 1129 1967 2322 Size: 1129 838 355 Fragments arranged by size: 1129 838 355 ApaI G_GGCC'C Cuts at: 0 359 835 2322 Size: 359 476 1487 Fragments arranged by size: 1487 476 359 ApaLI G'TGCA_C Cuts at: 0 1182 2322 Size: 1182 1140 ApoI r'AATT_y Cuts at: 0 499 675 2322 Size: 499 176 1647 Fragments arranged by size: 1647 499 176 AvaI C'yCGr_G Cuts at: 0 100 936 1533 2322 Size: 100 836 597 789 Fragments arranged by size: 836 789 597 100 BanI G'GyrC_C Cuts at: 0 241 991 1933 2322 Size: 241 750 942 389 Fragments arranged by size: 942 750 389 241 BanII G_rGCy'C Cuts at: 0 359 835 2297 2322 Size: 359 476 1462 25 Fragments arranged by size: 1462 476 359 25 BbsI GAAGACnn'nnnn_ Cuts at: 0 909 2322 Size: 909 1413 BbvCI CC'TCA_GC Cuts at: 0 1746 2322 Size: 1746 576 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1237 1271 2322 Size: 1237 34 1051 Fragments arranged by size: 1237 1051 34 BclI T'GATC_A Cuts at: 0 515 2322 Size: 515 1807 BglI GCCn_nnn'nGGC Cuts at: 0 157 239 1120 1313 1439 1595 2322 Size: 157 82 881 193 126 156 727 Fragments arranged by size: 881 727 193 157 156 126 82 BglII A'GATC_T Cuts at: 0 647 2322 Size: 647 1675 Bme1580I G_kGCm'C Cuts at: 0 244 359 835 1186 2111 2322 Size: 244 115 476 351 925 211 Fragments arranged by size: 925 476 351 244 211 115 BmgBI CAC'GTC Cuts at: 0 282 1581 2106 2322 Size: 282 1299 525 216 Fragments arranged by size: 1299 525 282 216 BmrI ACTGGGnnnn_n' Cuts at: 0 592 1150 1410 2322 Size: 592 558 260 912 Fragments arranged by size: 912 592 558 260 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 589 2322 Size: 589 1733 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 801 1708 2135 2322 Size: 801 907 427 187 Fragments arranged by size: 907 801 427 187 Bpu10I CC'TnA_GC Cuts at: 0 64 1746 2322 Size: 64 1682 576 Fragments arranged by size: 1682 576 64 BsaI GGTCTCn'nnnn_ Cuts at: 0 1465 2322 Size: 1465 857 BsaAI yAC'GTr Cuts at: 0 306 615 2322 Size: 306 309 1707 Fragments arranged by size: 1707 309 306 BsaHI Gr'CG_yC Cuts at: 0 431 2322 Size: 431 1891 BsaWI w'CCGG_w Cuts at: 0 944 2322 Size: 944 1378 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 247 277 301 331 1462 1492 2322 Size: 247 30 24 30 1131 30 830 Fragments arranged by size: 1131 830 247 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 324 1264 1741 1788 2322 Size: 324 940 477 47 534 Fragments arranged by size: 940 534 477 324 47 BseYI C'CCAG_C Cuts at: 0 181 297 425 828 1098 1437 2054 2322 Size: 181 116 128 403 270 339 617 268 Fragments arranged by size: 617 403 339 270 268 181 128 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 492 777 1624 1690 2322 Size: 492 285 847 66 632 Fragments arranged by size: 847 632 492 285 66 BsiEI CG_ry'CG Cuts at: 0 890 1320 1848 2322 Size: 890 430 528 474 Fragments arranged by size: 890 528 474 430 BsiHKAI G_wGCw'C Cuts at: 0 19 1025 1186 1534 2285 2322 Size: 19 1006 161 348 751 37 Fragments arranged by size: 1006 751 348 161 37 19 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2257 2322 Size: 2257 65 Bsp1286I G_dGCh'C Cuts at: 0 19 244 359 835 1025 1186 1534 2111 Size: 19 225 115 476 190 161 348 577 Cuts at: 2111 2285 2297 2322 Size: 174 12 25 Fragments arranged by size: 577 476 348 225 190 174 161 115 25 19 12 BspEI T'CCGG_A Cuts at: 0 944 2322 Size: 944 1378 BspHI T'CATG_A Cuts at: 0 1514 2186 2322 Size: 1514 672 136 Fragments arranged by size: 1514 672 136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 877 903 1925 2322 Size: 877 26 1022 397 Fragments arranged by size: 1022 877 397 26 BsrDI GCAATG_nn' Cuts at: 0 1197 2322 Size: 1197 1125 BsrFI r'CCGG_y Cuts at: 0 117 796 885 1694 1781 2322 Size: 117 679 89 809 87 541 Fragments arranged by size: 809 679 541 117 89 87 BsrGI T'GTAC_A Cuts at: 0 737 2228 2322 Size: 737 1491 94 Fragments arranged by size: 1491 737 94 BssSI C'ACGA_G Cuts at: 0 1246 2322 Size: 1246 1076 BstAPI GCAn_nnn'nTGC Cuts at: 0 248 2322 Size: 248 2074 BstXI CCAn_nnnn'nTGG Cuts at: 0 385 1686 1816 2245 2322 Size: 385 1301 130 429 77 Fragments arranged by size: 1301 429 385 130 77 BstYI r'GATC_y Cuts at: 0 647 1175 1805 1859 2322 Size: 647 528 630 54 463 Fragments arranged by size: 647 630 528 463 54 BtgI C'CryG_G Cuts at: 0 785 890 1283 1418 1721 1769 2045 2322 Size: 785 105 393 135 303 48 276 277 Fragments arranged by size: 785 393 303 277 276 135 105 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 266 2322 Size: 266 2056 BtsI GCAGTG_nn' Cuts at: 0 1557 1720 2322 Size: 1557 163 602 Fragments arranged by size: 1557 602 163 DraIII CAC_nnn'GTG Cuts at: 0 248 2322 Size: 248 2074 EaeI y'GGCC_r Cuts at: 0 158 231 668 775 887 1076 1317 1392 Size: 158 73 437 107 112 189 241 75 Cuts at: 1392 1818 1845 2211 2322 Size: 426 27 366 111 Fragments arranged by size: 437 426 366 241 189 158 112 111 107 75 73 27 EagI C'GGCC_G Cuts at: 0 887 1317 1845 2322 Size: 887 430 528 477 Fragments arranged by size: 887 528 477 430 EarI CTCTTCn'nnn_ Cuts at: 0 184 2255 2322 Size: 184 2071 67 Fragments arranged by size: 2071 184 67 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1613 2322 Size: 1613 709 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 801 903 1708 2135 2238 2271 2322 Size: 801 102 805 427 103 33 51 Fragments arranged by size: 805 801 427 103 102 51 33 EcoNI CCTnn'n_nnAGG Cuts at: 0 756 2128 2322 Size: 756 1372 194 Fragments arranged by size: 1372 756 194 EcoO109I rG'GnC_Cy Cuts at: 0 144 355 832 1065 1367 2322 Size: 144 211 477 233 302 955 Fragments arranged by size: 955 477 302 233 211 144 EcoRI G'AATT_C Cuts at: 0 675 2322 Size: 675 1647 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1980 2322 Size: 1980 342 HaeII r_GCGC'y Cuts at: 0 771 2322 Size: 771 1551 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 247 279 301 333 919 951 1057 1089 Size: 247 32 22 32 586 32 106 32 Cuts at: 1089 2322 Size: 1233 Fragments arranged by size: 1233 586 247 106 32 32 32 32 22 HincII GTy'rAC Cuts at: 0 1497 2094 2322 Size: 1497 597 228 Fragments arranged by size: 1497 597 228 MluI A'CGCG_T Cuts at: 0 1853 2322 Size: 1853 469 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1850 2201 2322 Size: 1850 351 121 Fragments arranged by size: 1850 351 121 MscI TGG'CCA Cuts at: 0 160 233 777 1394 1820 2322 Size: 160 73 544 617 426 502 Fragments arranged by size: 617 544 502 426 160 73 MslI CAynn'nnrTG Cuts at: 0 598 1490 1814 1897 1939 2044 2104 2224 Size: 598 892 324 83 42 105 60 120 Cuts at: 2224 2243 2322 Size: 19 79 Fragments arranged by size: 892 598 324 120 105 83 79 60 42 19 MspA1I CmG'CkG Cuts at: 0 32 86 171 821 1437 2291 2310 2322 Size: 32 54 85 650 616 854 19 12 Fragments arranged by size: 854 650 616 85 54 32 19 12 NaeI GCC'GGC Cuts at: 0 1783 2322 Size: 1783 539 NcoI C'CATG_G Cuts at: 0 785 1283 1418 1721 2045 2322 Size: 785 498 135 303 324 277 Fragments arranged by size: 785 498 324 303 277 135 NgoMIV G'CCGG_C Cuts at: 0 1781 2322 Size: 1781 541 NotI GC'GGCC_GC Cuts at: 0 1317 2322 Size: 1317 1005 NspI r_CATG'y Cuts at: 0 413 699 2064 2322 Size: 413 286 1365 258 Fragments arranged by size: 1365 413 286 258 PasI CC'CwG_GG Cuts at: 0 709 1593 2322 Size: 709 884 729 Fragments arranged by size: 884 729 709 PciI A'CATG_T Cuts at: 0 2060 2322 Size: 2060 262 PflMI CCAn_nnn'nTGG Cuts at: 0 320 425 1893 2322 Size: 320 105 1468 429 Fragments arranged by size: 1468 429 320 105 PfoI T'CCnGG_A Cuts at: 0 319 1466 2322 Size: 319 1147 856 Fragments arranged by size: 1147 856 319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1723 1755 2322 Size: 1723 32 567 Fragments arranged by size: 1723 567 32 PpuMI rG'GwC_Cy Cuts at: 0 1065 1367 2322 Size: 1065 302 955 Fragments arranged by size: 1065 955 302 PspOMI G'GGCC_C Cuts at: 0 355 831 2322 Size: 355 476 1491 Fragments arranged by size: 1491 476 355 PstI C_TGCA'G Cuts at: 0 31 1093 1715 2322 Size: 31 1062 622 607 Fragments arranged by size: 1062 622 607 31 PvuII CAG'CTG Cuts at: 0 32 86 171 821 1437 2310 2322 Size: 32 54 85 650 616 873 12 Fragments arranged by size: 873 650 616 85 54 32 12 SanDI GG'GwC_CC Cuts at: 0 1367 2322 Size: 1367 955 ScaI AGT'ACT Cuts at: 0 106 2322 Size: 106 2216 SexAI A'CCwGG_T Cuts at: 0 1067 1474 1646 2322 Size: 1067 407 172 676 Fragments arranged by size: 1067 676 407 172 SfcI C'TryA_G Cuts at: 0 27 1089 1711 2322 Size: 27 1062 622 611 Fragments arranged by size: 1062 622 611 27 SmlI C'TyrA_G Cuts at: 0 936 2322 Size: 936 1386 SphI G_CATG'C Cuts at: 0 413 2322 Size: 413 1909 StuI AGG'CCT Cuts at: 0 9 2322 Size: 9 2313 StyI C'CwwG_G Cuts at: 0 785 1283 1418 1721 1937 2045 2322 Size: 785 498 135 303 216 108 277 Fragments arranged by size: 785 498 303 277 216 135 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1774 1921 2322 Size: 1774 147 401 Fragments arranged by size: 1774 401 147 TatI w'GTAC_w Cuts at: 0 104 221 382 737 2228 2322 Size: 104 117 161 355 1491 94 Fragments arranged by size: 1491 355 161 117 104 94 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1374 1406 2322 Size: 1374 32 916 Fragments arranged by size: 1374 916 32 Tth111I GACn'n_nGTC Cuts at: 0 1648 2322 Size: 1648 674 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 825 1968 2322 Size: 825 1143 354 Fragments arranged by size: 1143 825 354 XhoI C'TCGA_G Cuts at: 0 936 2322 Size: 936 1386 Enzymes that do cut: AarI AcuI AfeI AflIII AhdI AleI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI BclI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII MluI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SanDI ScaI SexAI SfcI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BfrBI BlpI BmtI BpuEI BsaBI BsiWI BsmI BsrBI BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MfeI NarI NdeI NheI NruI NsiI PacI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XbaI XmaI XmnI ZraI