################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BciVI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HinfI HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 55 GTCTAC GT^[AC][GT]AC AfaI 189 GTAC GT^AC Aor51HI 368 AGCGCT AGC^GCT ApaI 167 GGGCCC GGGCC^C Asp700I 915 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 940 GAGCTC G[AT]GC[AT]^C BalI 554 TGGCCA TGG^CCA BbvCI 537 CCTCAGC CCTCAGC(-5/-2) BcgI 790 GCAGTGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 267 TGATCA T^GATCA BfaI 436 CTAG C^TAG BglII 149 AGATCT A^GATCT BsiHKAI 940 GAGCTC G[AT]GC[AT]^C BsmAI 638 GTCTC GTCTC(1/5) Bsp1407I 188 TGTACA T^GTACA BsrBI 694 GAGCGG CCGCTC(-3/-3) BsrFI 926 GCCGGC [GA]^CCGG[CT] BsrGI 188 TGTACA T^GTACA BstYI 149 AGATCT [GA]^GATC[CT] Cfr10I 926 GCCGGC [GA]^CCGG[CT] DdeI 538 CTCAG C^T[ACGT]AG DraII 167 GGGCCCT [GA]G^G[ACGT]CC[CT] DsaI 722 CCGTGG C^C[GA][CT]GG Eco47III 368 AGCGCT AGC^GCT EcoO109I 167 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 261 CCAAGG C^C[AT][AT]GG FbaI 267 TGATCA T^GATCA HaeII 368 AGCGCT [GA]GCGC^[CT] MaeI 436 CTAG C^TAG MflI 149 AGATCT [GA]^GATC[CT] MluNI 554 TGGCCA TGG^CCA MscI 554 TGGCCA TGG^CCA NaeI 926 GCCGGC GCC^GGC NgoMIV 926 GCCGGC G^CCGGC RsaI 189 GTAC GT^AC SacI 940 GAGCTC GAGCT^C SfcI 946 CTACAG C^T[GA][CT]AG SfiI 711 GGCCCGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 925 CGCCGGCG C[GA]^CCGG[CT]G SspBI 188 TGTACA T^GTACA StuI 830 AGGCCT AGG^CCT StyI 261 CCAAGG C^C[AT][AT]GG TaqI 799 TCGA T^CGA Tsp45I 463 GTGAC ^GT[GC]AC TthHB8I 799 TCGA T^CGA XhoII 149 AGATCT [GA]^GATC[CT] XmnI 915 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 61 CGCG CG^CG 857 CGCG AcyI 801 GACGCC G[GA]^CG[CT]C 808 GGCGTC AvaII 303 GGTCC G^G[AT]CC 888 GGACC BanII 167 GGGCCC G[GA]GC[CT]^C 940 GAGCTC BmyI 167 GGGCCC G[AGT]GC[ACT]^C 940 GAGCTC BsaHI 801 GACGCC G[GA]^CG[CT]C 808 GGCGTC BsiEI 182 CGGCCG CG[GA][CT]^CG 859 CGGCCG Bsp1286I 167 GGGCCC G[AGT]GC[ACT]^C 940 GAGCTC BstAPI 75 GCAACTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 521 GCACTGTCTGC BstUI 61 CGCG CG^CG 857 CGCG CfoI 369 GCGC GCG^C 795 GCGC DpnII 150 GATC ^GATC 268 GATC EagI 182 CGGCCG C^GGCCG 859 CGGCCG EclXI 182 CGGCCG C^GGCCG 859 CGGCCG Eco52I 182 CGGCCG C^GGCCG 859 CGGCCG HgaI 801 GACGC GACGC(5/10) 809 GCGTC HhaI 369 GCGC GCG^C 795 GCGC Hin1I 801 GACGCC G[GA]^CG[CT]C 808 GGCGTC HinP1I 369 GCGC G^CGC 795 GCGC MaeII 157 ACGT A^CGT 411 ACGT MaeIII 463 GTGAC ^GT[ACGT]AC 515 GTAAC MboI 150 GATC ^GATC 268 GATC MvnI 61 CGCG CG^CG 857 CGCG NdeII 150 GATC ^GATC 268 GATC PflMI 24 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 387 CCACCCGCTGG Sau3AI 150 GATC ^GATC 268 GATC Van91I 24 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 387 CCACCCGCTGG XcmI 690 CCAGGAGCGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 875 CCAACAGCGTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AluI AlwNI BbvI BcnI BglI BsaJI BsgI BsiYI BslI BsmFI BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I EaeI EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI Ksp632I MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI TseI TspRI