(Circular) (Six-base) MAPSORT of: pFN21AE1091.seq Check: 3846 from: 1 to: 5689 pFN21AE1091 5689 bp With 182 enzymes: SgfI * October 14, 2009 13:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2366 2366 Size: 5689 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2061 3032 1937 Size: 124 971 4594 Fragments arranged by size: 4594 971 124 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3752 5136 5404 1632 Size: 2120 1384 268 1917 Fragments arranged by size: 2120 1917 1384 268 AfeI AGC'GCT Cuts at: 2375 2375 Size: 5689 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 4588 5565 1203 Size: 3385 977 1327 Fragments arranged by size: 3385 1327 977 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 2189 2415 1166 Size: 1023 226 4440 Fragments arranged by size: 4440 1023 226 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5689 AlwNI CAG_nnn'CTG Cuts at: 2035 2482 2687 3432 5004 2035 Size: 447 205 745 1572 2720 Fragments arranged by size: 2720 1572 745 447 205 ApaI G_GGCC'C Cuts at: 2176 2176 Size: 5689 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2998 3224 3376 5485 1493 Size: 1505 226 152 2109 1697 Fragments arranged by size: 2109 1697 1505 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 3014 1870 Size: 73 1071 4545 Fragments arranged by size: 4545 1071 73 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3010 4465 618 Size: 325 217 627 1223 1455 1842 Fragments arranged by size: 1842 1455 1223 627 325 217 BanII G_rGCy'C Cuts at: 1391 2176 2949 3008 1391 Size: 785 773 59 4072 Fragments arranged by size: 4072 785 773 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4718 BbvCI CC'TCA_GC Cuts at: 2543 2543 Size: 5689 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2784 2818 3880 3914 1903 Size: 34 847 34 1062 34 3678 Fragments arranged by size: 3678 1062 847 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 2272 1548 Size: 724 4965 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2630 2673 2723 Size: 107 122 71 631 605 958 43 50 Cuts at: 2723 2792 4300 136 Size: 69 1508 1525 Fragments arranged by size: 1525 1508 958 631 605 122 107 71 69 50 43 BglII A'GATC_T Cuts at: 2154 5684 2154 Size: 3530 2159 BlpI GC'TnA_GC Cuts at: 3106 3106 Size: 5689 Bme1580I G_kGCm'C Cuts at: 1790 2176 3750 4906 1790 Size: 386 1574 1156 2573 Fragments arranged by size: 2573 1574 1156 386 BmgBI CAC'GTC Cuts at: 1318 2163 1318 Size: 845 4844 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2470 3420 4378 Size: 416 53 83 399 24 1053 950 958 Cuts at: 4378 442 Size: 1753 Fragments arranged by size: 1753 1053 958 950 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4349 1239 Size: 3110 2579 Bpu10I CC'TnA_GC Cuts at: 2543 2543 Size: 5689 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3814 4679 4977 3814 Size: 865 298 4526 Fragments arranged by size: 4526 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4352 882 Size: 617 2853 2219 Fragments arranged by size: 2853 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3480 493 Size: 711 2276 2702 Fragments arranged by size: 2702 2276 711 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2807 2814 3496 3878 Size: 53 83 186 936 1274 7 682 382 Cuts at: 3878 4513 275 Size: 635 1451 Fragments arranged by size: 1451 1274 936 682 635 382 186 83 53 7 BsaWI w'CCGG_w Cuts at: 4122 4520 4794 4941 5352 4122 Size: 398 274 147 411 4459 Fragments arranged by size: 4459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2931 2961 1178 Size: 30 165 30 1528 30 3906 Fragments arranged by size: 3906 1528 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5689 BseYI C'CCAG_C Cuts at: 4892 5388 4892 Size: 496 5193 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2143 2318 2806 1163 Size: 573 407 175 488 4046 Fragments arranged by size: 4046 573 488 407 175 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2190 2867 3139 3900 4928 1540 Size: 275 186 189 677 272 761 1028 2301 Fragments arranged by size: 2301 1028 761 677 275 272 189 186 BsiHKAI G_wGCw'C Cuts at: 2949 3750 3835 4906 2949 Size: 801 85 1071 3732 Fragments arranged by size: 3732 1071 801 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2176 2949 3008 3750 3835 4906 1391 Size: 399 386 773 59 742 85 1071 2174 Fragments arranged by size: 2174 1071 773 742 399 386 85 59 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 3578 5308 3578 Size: 1730 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2229 2340 2366 3044 844 Size: 1385 111 26 678 3489 Fragments arranged by size: 3489 1385 678 111 26 BsrBI CCG'CTC Cuts at: 1394 1823 2701 3576 1394 Size: 429 878 875 3507 Fragments arranged by size: 3507 878 875 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2931 4333 4520 1700 Size: 110 1121 1402 187 2869 Fragments arranged by size: 2869 1402 1121 187 110 BsrGI T'GTAC_A Cuts at: 96 2193 96 Size: 2097 3592 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 3749 4761 3749 Size: 1012 4677 BstAPI GCAn_nnn'nTGC Cuts at: 794 2086 2532 794 Size: 1292 446 3951 Fragments arranged by size: 3951 1292 446 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstYI r'GATC_y Cuts at: 1972 2154 3019 3386 3771 3788 5240 5326 5338 Size: 182 865 367 385 17 1452 86 12 Cuts at: 5338 5684 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 865 385 367 346 182 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2727 513 Size: 552 939 723 3475 Fragments arranged by size: 3475 939 723 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4950 Fragments arranged by size: 4950 683 56 BtsI GCAGTG_nn' Cuts at: 2802 3205 3999 4019 2802 Size: 403 794 20 4472 Fragments arranged by size: 4472 794 403 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 2463 2559 Size: 54 1425 50 275 18 357 276 96 Cuts at: 2559 2724 2864 3136 4025 5654 8 Size: 165 140 272 889 1629 43 Fragments arranged by size: 1629 1425 889 357 276 275 272 165 140 96 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2187 2864 3136 1537 Size: 275 375 677 272 4090 Fragments arranged by size: 4090 677 375 275 272 EarI CTCTTCn'nnn_ Cuts at: 1353 2324 2354 2909 3619 1353 Size: 971 30 555 710 3423 Fragments arranged by size: 3423 971 710 555 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2918 4276 4650 4796 387 Size: 1064 138 1329 1358 374 146 1280 Fragments arranged by size: 1358 1329 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2947 2947 Size: 5689 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3752 4349 5136 5404 1239 Size: 393 2120 597 787 268 1524 Fragments arranged by size: 2120 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2173 2173 Size: 5689 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 2377 4836 1678 Size: 699 2459 2531 Fragments arranged by size: 2531 2459 699 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2968 3000 4330 4362 4404 Size: 32 135 32 1396 32 1330 32 42 Cuts at: 4404 4436 1373 Size: 32 2626 Fragments arranged by size: 2626 1396 1330 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4803 4987 1305 Size: 3498 184 2007 Fragments arranged by size: 3498 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2561 10 Size: 54 1425 343 729 3138 Fragments arranged by size: 3138 1425 729 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2189 2415 2726 3647 4006 4165 518 Size: 648 1023 226 311 921 359 159 2042 Fragments arranged by size: 2042 1023 921 648 359 311 226 159 MspA1I CmG'CkG Cuts at: 1606 2131 2398 2846 3105 3782 4930 5175 1606 Size: 525 267 448 259 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 525 448 267 259 245 NaeI GCC'GGC Cuts at: 2933 2933 Size: 5689 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4198 Fragments arranged by size: 4198 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NgoMIV G'CCGG_C Cuts at: 2931 2931 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 3047 4592 1132 Size: 97 1818 1545 2229 Fragments arranged by size: 2229 1818 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2035 2398 1108 Size: 902 25 363 4399 Fragments arranged by size: 4399 902 363 25 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PspOMI G'GGCC_C Cuts at: 2172 2172 Size: 5689 PstI C_TGCA'G Cuts at: 830 3041 830 Size: 2211 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 SacI G_AGCT'C Cuts at: 2949 2949 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SfcI C'TryA_G Cuts at: 826 1045 2951 3037 4172 4853 5044 826 Size: 219 1906 86 1135 681 191 1471 Fragments arranged by size: 1906 1471 1135 681 219 191 86 SfiI GGCCn_nnn'nGGCC Cuts at: 2723 2723 Size: 5689 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 2931 1700 Size: 1231 4458 SmaI CCC'GGG Cuts at: 1872 3016 1872 Size: 1144 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3793 4694 4956 820 Size: 197 926 1850 901 262 1553 Fragments arranged by size: 1850 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 2837 1517 Size: 1320 4369 StyI C'CwwG_G Cuts at: 513 1065 2004 2266 3128 513 Size: 552 939 262 862 3074 Fragments arranged by size: 3074 939 862 552 262 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2062 2579 2930 3716 3733 3886 4071 Size: 1255 482 517 351 786 17 153 185 Cuts at: 4071 4516 325 Size: 445 1498 Fragments arranged by size: 1498 1255 786 517 482 445 351 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2193 3935 5647 Size: 274 80 33 51 1443 216 1742 1712 Cuts at: 5647 96 Size: 138 Fragments arranged by size: 1742 1712 1443 274 216 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2931 2963 5649 5681 2931 Size: 32 2686 32 2939 Fragments arranged by size: 2939 2686 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2702 2887 2702 Size: 185 5504 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 3014 1870 Size: 1144 4545 XmnI GAAnn'nnTTC Cuts at: 2924 3818 2924 Size: 894 4795 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI