(Circular) (Six-base) MAPSORT of: pFN21AE1328.seq Check: 4306 from: 1 to: 5797 pFN21AE1328 5797 bp With 182 enzymes: SgfI * July 14, 2018 22:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2576 2576 Size: 5797 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3607 278 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3140 1937 Size: 1203 4594 Acc65I G'GTAC_C Cuts at: 2649 3118 2649 Size: 469 5328 AclI AA'CG_TT Cuts at: 1563 3924 4297 1563 Size: 2361 373 3063 Fragments arranged by size: 3063 2361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2147 2618 3860 5244 5512 1632 Size: 515 471 1242 1384 268 1917 Fragments arranged by size: 1917 1384 1242 515 471 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5600 AflIII A'CryG_T Cuts at: 1203 2073 2075 4696 5673 1203 Size: 870 2 2621 977 1327 Fragments arranged by size: 2621 1327 977 870 2 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 4526 4526 Size: 5797 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5797 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2558 1184 Size: 1374 4423 AlwNI CAG_nnn'CTG Cuts at: 3540 5112 3540 Size: 1572 4225 ApaLI G'TGCA_C Cuts at: 2533 3854 5010 2533 Size: 1321 1156 3320 Fragments arranged by size: 3320 1321 1156 ApoI r'AATT_y Cuts at: 1493 3106 3332 3484 5593 1493 Size: 1613 226 152 2109 1697 Fragments arranged by size: 2109 1697 1613 226 152 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 2426 3122 1870 Size: 73 483 696 4545 Fragments arranged by size: 4545 696 483 73 BamHI G'GATC_C Cuts at: 3127 3494 3127 Size: 367 5430 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2204 2649 3118 4573 618 Size: 325 217 627 417 445 469 1455 1842 Fragments arranged by size: 1842 1455 627 469 445 417 325 217 BanII G_rGCy'C Cuts at: 1391 2706 3029 3116 1391 Size: 1315 323 87 4072 Fragments arranged by size: 4072 1315 323 87 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2061 2236 928 Size: 971 162 175 4489 Fragments arranged by size: 4489 971 175 162 BbvCI CC'TCA_GC Cuts at: 2973 2973 Size: 5797 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3988 4022 1903 Size: 34 2051 34 3678 Fragments arranged by size: 3678 2051 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3689 4899 3689 Size: 1210 4587 BclI T'GATC_A Cuts at: 1548 1548 Size: 5797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4408 136 Size: 107 122 71 631 605 2736 1525 Fragments arranged by size: 2736 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 2399 3214 2399 Size: 815 4982 Bme1580I G_kGCm'C Cuts at: 1790 2207 2537 3858 5014 1790 Size: 417 330 1321 1156 2573 Fragments arranged by size: 2573 1321 1156 417 330 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5797 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3014 3528 4486 Size: 416 53 83 399 24 1597 514 958 Cuts at: 4486 442 Size: 1753 Fragments arranged by size: 1753 1597 958 514 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5797 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2405 2720 4457 1239 Size: 1166 315 1737 2579 Fragments arranged by size: 2579 1737 1166 315 Bpu10I CC'TnA_GC Cuts at: 2973 2973 Size: 5797 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2603 3922 4787 5085 2603 Size: 1319 865 298 3315 Fragments arranged by size: 3315 1319 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2931 3040 4460 882 Size: 617 1432 109 1420 2219 Fragments arranged by size: 2219 1432 1420 617 109 BsaAI yAC'GTr Cuts at: 493 1204 2076 3588 493 Size: 711 872 1512 2702 Fragments arranged by size: 2702 1512 872 711 BsaBI GATnn'nnATC Cuts at: 5791 5791 Size: 5797 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3604 3986 4621 275 Size: 53 83 186 936 2071 382 635 1451 Fragments arranged by size: 2071 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4230 4628 4902 5049 5460 4230 Size: 398 274 147 411 4567 Fragments arranged by size: 4567 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5572 Fragments arranged by size: 5572 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2182 2543 1186 Size: 996 361 4440 Fragments arranged by size: 4440 996 361 BseYI C'CCAG_C Cuts at: 2026 2379 5000 5496 2026 Size: 353 2621 496 2327 Fragments arranged by size: 2621 2327 496 353 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5224 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3247 4008 5036 1540 Size: 275 186 1246 761 1028 2301 Fragments arranged by size: 2301 1246 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2537 2706 3029 3858 3943 5014 2537 Size: 169 323 829 85 1071 3320 Fragments arranged by size: 3320 1071 829 323 169 85 BsmI GAATG_Cn' Cuts at: 3308 3401 3308 Size: 93 5704 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2207 2537 2706 3029 3116 3858 3943 Size: 399 417 330 169 323 87 742 85 Cuts at: 3943 5014 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 417 399 330 323 169 87 85 BspEI T'CCGG_A Cuts at: 5460 5460 Size: 5797 BspHI T'CATG_A Cuts at: 3686 5416 3686 Size: 1730 4067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2550 2576 3152 844 Size: 1706 26 576 3489 Fragments arranged by size: 3489 1706 576 26 BsrBI CCG'CTC Cuts at: 1394 1823 3684 1394 Size: 429 1861 3507 Fragments arranged by size: 3507 1861 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4292 4466 65 Size: 1183 3044 174 1396 Fragments arranged by size: 3044 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4441 4628 1700 Size: 110 2631 187 2869 Fragments arranged by size: 2869 2631 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5797 BssSI C'ACGA_G Cuts at: 3857 4869 3857 Size: 1012 4785 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5797 BstBI TT'CG_AA Cuts at: 4601 4601 Size: 5797 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5797 BstXI CCAn_nnnn'nTGG Cuts at: 2038 2949 2038 Size: 911 4886 BstYI r'GATC_y Cuts at: 1972 2011 3127 3494 3879 3896 5348 5434 5446 Size: 39 1116 367 385 17 1452 86 12 Cuts at: 5446 5792 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1116 385 367 346 86 39 17 12 Bsu36I CC'TnA_GG Cuts at: 2194 2194 Size: 5797 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5245 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5058 Fragments arranged by size: 5058 683 56 BtsI GCAGTG_nn' Cuts at: 3313 4107 4127 3313 Size: 794 20 4983 Fragments arranged by size: 4983 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 2839 3101 2839 Size: 262 5535 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2240 4804 809 Size: 507 924 2564 1802 Fragments arranged by size: 2564 1802 924 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3244 4133 5762 Size: 54 1425 50 275 18 1414 889 1629 Cuts at: 5762 8 Size: 43 Fragments arranged by size: 1629 1425 1414 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3244 1537 Size: 275 1432 4090 Fragments arranged by size: 4090 1432 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3727 1353 Size: 2374 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2621 2924 4384 4758 4904 387 Size: 1064 138 1032 303 1460 374 146 1280 Fragments arranged by size: 1460 1280 1064 1032 374 303 146 138 EcoICRI GAG'CTC Cuts at: 2704 3027 2704 Size: 323 5474 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2405 2618 2720 3860 4457 5244 Size: 393 515 258 213 102 1140 597 787 Cuts at: 5244 5512 1239 Size: 268 1524 Fragments arranged by size: 1524 1140 787 597 515 393 268 258 213 102 EcoO109I rG'GnC_Cy Cuts at: 3015 3015 Size: 5797 EcoRI G'AATT_C Cuts at: 3106 3484 3106 Size: 378 5419 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 2608 4944 1678 Size: 930 2336 2531 Fragments arranged by size: 2531 2336 930 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2283 2315 4438 4470 4512 Size: 32 135 32 711 32 2123 32 42 Cuts at: 4512 4544 1373 Size: 32 2626 Fragments arranged by size: 2626 2123 711 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2481 3141 3387 669 Size: 988 281 543 660 246 3079 Fragments arranged by size: 3079 988 660 543 281 246 HindIII A'AGCT_T Cuts at: 748 2669 748 Size: 1921 3876 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KpnI G_GTAC'C Cuts at: 2653 3122 2653 Size: 469 5328 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2930 4911 5095 1305 Size: 1625 1981 184 2007 Fragments arranged by size: 2007 1981 1625 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3975 Fragments arranged by size: 3975 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3755 4114 4273 518 Size: 648 2589 359 159 2042 Fragments arranged by size: 2589 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2320 2959 3213 3890 5038 5283 1606 Size: 714 639 254 677 1148 245 2120 Fragments arranged by size: 2120 1148 714 677 639 254 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5245 NdeI CA'TA_TG Cuts at: 387 387 Size: 5797 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5797 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 2089 3155 4700 1132 Size: 97 860 1066 1545 2229 Fragments arranged by size: 2229 1545 1066 860 97 PasI CC'CwG_GG Cuts at: 1348 2470 2761 1348 Size: 1122 291 4384 Fragments arranged by size: 4384 1122 291 PciI A'CATG_T Cuts at: 4696 4696 Size: 5797 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5797 PfoI T'CCnGG_A Cuts at: 2301 2898 3029 2301 Size: 597 131 5069 Fragments arranged by size: 5069 597 131 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PmlI CAC'GTG Cuts at: 2076 2076 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2543 2575 3866 3898 5379 5411 2543 Size: 32 1291 32 1481 32 2929 Fragments arranged by size: 2929 1481 1291 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3015 3015 Size: 5797 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5797 PsiI TTA'TAA Cuts at: 3367 3367 Size: 5797 PstI C_TGCA'G Cuts at: 830 2547 3149 830 Size: 1717 602 3478 Fragments arranged by size: 3478 1717 602 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 PvuII CAG'CTG Cuts at: 2320 2959 2320 Size: 639 5158 SacI G_AGCT'C Cuts at: 2706 3029 2706 Size: 323 5474 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SanDI GG'GwC_CC Cuts at: 3015 3015 Size: 5797 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SexAI A'CCwGG_T Cuts at: 2235 2235 Size: 5797 SfcI C'TryA_G Cuts at: 826 1045 2543 2582 2916 3145 4280 4961 5152 Size: 219 1498 39 334 229 1135 681 191 Cuts at: 5152 826 Size: 1471 Fragments arranged by size: 1498 1471 1135 681 334 229 219 191 39 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 3124 1872 Size: 1252 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2618 3901 4802 5064 820 Size: 197 926 675 1283 901 262 1553 Fragments arranged by size: 1553 1283 926 901 675 262 197 SnaBI TAC'GTA Cuts at: 493 3588 493 Size: 3095 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 2089 3155 1132 Size: 957 1066 3774 Fragments arranged by size: 3774 1066 957 SspI AAT'ATT Cuts at: 5 52 3721 5 Size: 47 3669 2081 Fragments arranged by size: 3669 2081 47 StuI AGG'CCT Cuts at: 1517 2890 2978 1517 Size: 1373 88 4336 Fragments arranged by size: 4336 1373 88 StyI C'CwwG_G Cuts at: 513 1065 2172 3236 513 Size: 552 1107 1064 3074 Fragments arranged by size: 3074 1107 1064 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2918 3824 3841 3994 4179 4624 325 Size: 1255 1338 906 17 153 185 445 1498 Fragments arranged by size: 1498 1338 1255 906 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4043 5755 96 Size: 274 80 33 51 1443 2066 1712 138 Fragments arranged by size: 2066 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5757 5789 5757 Size: 32 5765 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5797 XbaI T'CTAG_A Cuts at: 3133 3133 Size: 5797 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3027 3027 Size: 5797 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 3122 1870 Size: 1252 4545 XmnI GAAnn'nnTTC Cuts at: 3926 3926 Size: 5797 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3605 276 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI