(Circular) (Six-base) MAPSORT of: pFN21AE2473.seq Check: 9357 from: 1 to: 7231 pFN21AE2473 7231 bp With 182 enzymes: SgfI * December 3, 2014 10:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3069 3069 Size: 7231 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5041 278 Size: 53 83 186 936 3505 2468 Fragments arranged by size: 3505 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2714 4574 1937 Size: 777 1860 4594 Fragments arranged by size: 4594 1860 777 Acc65I G'GTAC_C Cuts at: 4552 4552 Size: 7231 AclI AA'CG_TT Cuts at: 1563 5358 5731 1563 Size: 3795 373 3063 Fragments arranged by size: 3795 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2234 3202 3778 4164 4199 4521 5294 6678 Size: 602 968 576 386 35 322 773 1384 Cuts at: 6678 6946 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 968 773 602 576 386 322 268 35 AfeI AGC'GCT Cuts at: 3478 4272 3478 Size: 794 6437 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7034 AflIII A'CryG_T Cuts at: 1203 3693 3828 4464 6130 7107 1203 Size: 2490 135 636 1666 977 1327 Fragments arranged by size: 2490 1666 1327 977 636 135 AgeI A'CCGG_T Cuts at: 6062 6062 Size: 7231 AhdI GACnn_n'nnGTC Cuts at: 5960 5960 Size: 7231 AleI CACnn'nnGTG Cuts at: 1166 2096 2521 1166 Size: 930 425 5876 Fragments arranged by size: 5876 930 425 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3917 4265 1184 Size: 2733 348 4150 Fragments arranged by size: 4150 2733 348 AlwNI CAG_nnn'CTG Cuts at: 2410 3511 4974 6546 2410 Size: 1101 1463 1572 3095 Fragments arranged by size: 3095 1572 1463 1101 ApaI G_GGCC'C Cuts at: 2043 2308 2623 3750 3875 2043 Size: 265 315 1127 125 5399 Fragments arranged by size: 5399 1127 315 265 125 ApaLI G'TGCA_C Cuts at: 3788 5288 6444 3788 Size: 1500 1156 4575 Fragments arranged by size: 4575 1500 1156 ApoI r'AATT_y Cuts at: 1493 4540 4766 4918 7027 1493 Size: 3047 226 152 2109 1697 Fragments arranged by size: 3047 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5785 160 Size: 872 4753 1606 Fragments arranged by size: 4753 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7231 AvaI C'yCGr_G Cuts at: 1870 1943 2298 2769 3351 3524 4443 4556 1870 Size: 73 355 471 582 173 919 113 4545 Fragments arranged by size: 4545 919 582 471 355 173 113 73 BamHI G'GATC_C Cuts at: 3006 4561 4928 3006 Size: 1555 367 5309 Fragments arranged by size: 5309 1555 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2357 2848 2893 3084 3093 Size: 325 217 627 570 491 45 191 9 Cuts at: 3093 3605 3647 4552 6007 618 Size: 512 42 905 1455 1842 Fragments arranged by size: 1842 1455 905 627 570 512 491 325 217 191 45 42 9 BanII G_rGCy'C Cuts at: 1391 2043 2308 2623 2817 3072 3525 3531 3750 Size: 652 265 315 194 255 453 6 219 Cuts at: 3750 3875 4550 1391 Size: 125 675 4072 Fragments arranged by size: 4072 675 652 453 315 265 255 219 194 125 6 BbeI G_GCGC'C Cuts at: 2361 3097 2361 Size: 736 6495 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2926 4509 928 Size: 971 1027 1583 3650 Fragments arranged by size: 3650 1583 1027 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4321 4355 5422 5456 1903 Size: 34 2384 34 1067 34 3678 Fragments arranged by size: 3678 2384 1067 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4270 5123 6333 4270 Size: 853 1210 5168 Fragments arranged by size: 5168 1210 853 BclI T'GATC_A Cuts at: 1548 1548 Size: 7231 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2276 2280 2289 Size: 107 122 71 631 605 604 4 9 Cuts at: 2289 2303 4133 4301 5842 136 Size: 14 1830 168 1541 1525 Fragments arranged by size: 1830 1541 1525 631 605 604 168 122 107 71 14 9 4 BglII A'GATC_T Cuts at: 7226 7226 Size: 7231 BlpI GC'TnA_GC Cuts at: 3155 4184 4648 3155 Size: 1029 464 5738 Fragments arranged by size: 5738 1029 464 Bme1580I G_kGCm'C Cuts at: 1790 2043 2103 2294 2308 2623 2836 3134 3358 Size: 253 60 191 14 315 213 298 224 Cuts at: 3358 3750 3792 3875 4014 5292 6448 1790 Size: 392 42 83 139 1278 1156 2573 Fragments arranged by size: 2573 1278 1156 392 315 298 253 224 213 191 139 83 60 42 14 BmgBI CAC'GTC Cuts at: 1318 3027 3829 1318 Size: 1709 802 4720 Fragments arranged by size: 4720 1709 802 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2632 4962 5920 Size: 416 53 83 399 24 1215 2330 958 Cuts at: 5920 442 Size: 1753 Fragments arranged by size: 2330 1753 1215 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7231 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2799 2799 Size: 7231 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2363 2613 2933 3759 5891 1239 Size: 1124 250 320 826 2132 2579 Fragments arranged by size: 2579 2132 1124 826 320 250 Bpu10I CC'TnA_GC Cuts at: 4104 4104 Size: 7231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2924 4214 5356 6221 6519 2924 Size: 1290 1142 865 298 3636 Fragments arranged by size: 3636 1290 1142 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2456 2593 2618 3999 5894 882 Size: 617 957 137 25 1381 1895 2219 Fragments arranged by size: 2219 1895 1381 957 617 137 25 BsaAI yAC'GTr Cuts at: 493 1204 2224 5022 493 Size: 711 1020 2798 2702 Fragments arranged by size: 2798 2702 1020 711 BsaBI GATnn'nnATC Cuts at: 7225 7225 Size: 7231 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2158 2358 3094 5038 Size: 53 83 186 936 625 200 736 1944 Cuts at: 5038 5420 6055 275 Size: 382 635 1451 Fragments arranged by size: 1944 1451 936 736 635 625 382 200 186 83 53 BsaWI w'CCGG_w Cuts at: 3990 5664 6062 6336 6483 6894 3990 Size: 1674 398 274 147 411 4327 Fragments arranged by size: 4327 1674 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3455 3485 3680 3710 1178 Size: 30 165 30 2052 30 195 30 4699 Fragments arranged by size: 4699 2052 195 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2450 3521 3728 1186 Size: 1264 1071 207 4689 Fragments arranged by size: 4689 1264 1071 207 BseYI C'CCAG_C Cuts at: 4076 6434 6930 4076 Size: 2358 496 4377 Fragments arranged by size: 4377 2358 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3035 3047 1163 Size: 573 1299 12 5347 Fragments arranged by size: 5347 1299 573 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4066 4681 5442 6470 1540 Size: 275 186 2065 615 761 1028 2301 Fragments arranged by size: 2301 2065 1028 761 615 275 186 BsiHKAI G_wGCw'C Cuts at: 2802 3072 3600 3792 3896 5292 5377 6448 2802 Size: 270 528 192 104 1396 85 1071 3585 Fragments arranged by size: 3585 1396 1071 528 270 192 104 85 BsmI GAATG_Cn' Cuts at: 4742 4835 4742 Size: 93 7138 BsmBI CGTCTCn'nnnn_ Cuts at: 3033 3380 3033 Size: 347 6884 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2103 2294 2308 2623 2802 2817 Size: 399 253 60 191 14 315 179 15 Cuts at: 2817 2836 3072 3134 3358 3525 3531 3600 3750 Size: 19 236 62 224 167 6 69 150 Cuts at: 3750 3792 3875 3896 4014 4550 5292 5377 6448 Size: 42 83 21 118 536 742 85 1071 Cuts at: 6448 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 536 399 315 253 236 224 191 179 167 150 118 85 83 69 62 60 42 21 19 15 14 6 BspEI T'CCGG_A Cuts at: 6894 6894 Size: 7231 BspHI T'CATG_A Cuts at: 2659 5120 6850 2659 Size: 2461 1730 3040 Fragments arranged by size: 3040 2461 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2542 2644 2997 3069 4586 844 Size: 1698 102 353 72 1517 3489 Fragments arranged by size: 3489 1698 1517 353 102 72 BsrBI CCG'CTC Cuts at: 1394 1823 2167 2389 4171 4188 5118 1394 Size: 429 344 222 1782 17 930 3507 Fragments arranged by size: 3507 1782 930 429 344 222 17 BsrDI GCAATG_nn' Cuts at: 65 1248 2421 2648 4212 5726 5900 65 Size: 1183 1173 227 1564 1514 174 1396 Fragments arranged by size: 1564 1514 1396 1183 1173 227 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2201 2270 2354 3536 5875 6062 1700 Size: 110 391 69 84 1182 2339 187 2869 Fragments arranged by size: 2869 2339 1182 391 187 110 84 69 BsrGI T'GTAC_A Cuts at: 96 3624 96 Size: 3528 3703 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7231 BssSI C'ACGA_G Cuts at: 2096 3065 3357 4427 5291 6303 2096 Size: 969 292 1070 864 1012 3024 Fragments arranged by size: 3024 1070 1012 969 864 292 BstAPI GCAn_nnn'nTGC Cuts at: 794 2025 794 Size: 1231 6000 BstBI TT'CG_AA Cuts at: 6035 6035 Size: 7231 BstEII G'GTnAC_C Cuts at: 1184 3973 1184 Size: 2789 4442 BstXI CCAn_nnnn'nTGG Cuts at: 2523 2957 4345 2523 Size: 434 1388 5409 Fragments arranged by size: 5409 1388 434 BstYI r'GATC_y Cuts at: 1972 3006 4383 4561 4928 5313 5330 6782 6868 Size: 1034 1377 178 367 385 17 1452 86 Cuts at: 6868 6880 7226 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1377 1034 385 367 346 178 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 4521 513 Size: 552 939 39 2478 3223 Fragments arranged by size: 3223 2478 939 552 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4027 487 Size: 683 56 2801 3691 Fragments arranged by size: 3691 2801 683 56 BtsI GCAGTG_nn' Cuts at: 2292 2309 3074 3369 4747 5541 5561 2292 Size: 17 765 295 1378 794 20 3962 Fragments arranged by size: 3962 1378 794 765 295 20 17 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7231 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7196 DraI TTT'AAA Cuts at: 4535 4535 Size: 7231 DraIII CAC_nnn'GTG Cuts at: 3303 3303 Size: 7231 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6238 809 Size: 507 4922 1802 Fragments arranged by size: 4922 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2241 2268 2281 Size: 54 1425 50 275 18 411 27 13 Cuts at: 2281 2352 3189 4063 4311 4678 5567 7196 8 Size: 71 837 874 248 367 889 1629 43 Fragments arranged by size: 1629 1425 889 874 837 411 367 275 248 71 54 50 43 27 18 13 EagI C'GGCC_G Cuts at: 1537 1812 4063 4678 1537 Size: 275 2251 615 4090 Fragments arranged by size: 4090 2251 615 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2151 2658 3884 5161 1353 Size: 798 507 1226 1277 3423 Fragments arranged by size: 3423 1277 1226 798 507 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2732 5818 6192 6338 387 Size: 1064 138 1143 3086 374 146 1280 Fragments arranged by size: 3086 1280 1143 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3070 3070 Size: 7231 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2363 2613 2933 3202 3759 3778 Size: 393 602 129 250 320 269 557 19 Cuts at: 3778 4164 4199 4521 5294 5891 6678 6946 1239 Size: 386 35 322 773 597 787 268 1524 Fragments arranged by size: 1524 787 773 602 597 557 393 386 322 320 269 268 250 129 35 19 EcoNI CCTnn'n_nnAGG Cuts at: 3655 3741 3655 Size: 86 7145 EcoO109I rG'GnC_Cy Cuts at: 2039 2304 2305 2620 3730 3746 3747 2039 Size: 265 1 315 1110 16 1 5523 Fragments arranged by size: 5523 1110 315 265 16 1 1 EcoRI G'AATT_C Cuts at: 4540 4918 4540 Size: 378 6853 FseI GG_CCGG'CC Cuts at: 2205 2274 2205 Size: 69 7162 FspI TGC'GCA Cuts at: 2993 3553 5737 2993 Size: 560 2184 4487 Fragments arranged by size: 4487 2184 560 HaeII r_GCGC'y Cuts at: 1678 2261 2361 3097 3480 4274 6378 1678 Size: 583 100 736 383 794 2104 2531 Fragments arranged by size: 2531 2104 794 736 583 383 100 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2174 2206 3109 3141 3426 Size: 32 135 32 602 32 903 32 285 Cuts at: 3426 3455 3458 3487 3917 3949 4250 4282 5872 Size: 29 3 29 430 32 301 32 1590 Cuts at: 5872 5904 5946 5978 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1590 903 602 430 301 285 135 42 32 32 32 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 4575 4821 669 Size: 988 281 2637 246 3079 Fragments arranged by size: 3079 2637 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7231 HpaI GTT'AAC Cuts at: 4821 4821 Size: 7231 KasI G'GCGC_C Cuts at: 2357 3093 2357 Size: 736 6495 KpnI G_GTAC'C Cuts at: 4556 4556 Size: 7231 MfeI C'AATT_G Cuts at: 3637 4830 3637 Size: 1193 6038 MluI A'CGCG_T Cuts at: 7107 7107 Size: 7231 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6345 6529 1305 Size: 5040 184 2007 Fragments arranged by size: 5040 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2243 4313 10 Size: 54 1425 343 411 2070 2928 Fragments arranged by size: 2928 2070 1425 411 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2096 2521 3683 3842 4343 5189 5548 Size: 648 930 425 1162 159 501 846 359 Cuts at: 5548 5707 518 Size: 159 2042 Fragments arranged by size: 2042 1162 930 846 648 501 425 359 159 159 MspA1I CmG'CkG Cuts at: 1606 2174 2233 2483 2791 3622 4076 4474 4647 Size: 568 59 250 308 831 454 398 173 Cuts at: 4647 5324 6472 6717 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 831 677 568 454 398 308 250 245 173 59 NaeI GCC'GGC Cuts at: 2203 2272 2356 3538 2203 Size: 69 84 1182 5896 Fragments arranged by size: 5896 1182 84 69 NarI GG'CG_CC Cuts at: 2358 3094 2358 Size: 736 6495 NcoI C'CATG_G Cuts at: 513 1065 2004 4521 513 Size: 552 939 2517 3223 Fragments arranged by size: 3223 2517 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7231 NgoMIV G'CCGG_C Cuts at: 2201 2270 2354 3536 2201 Size: 69 84 1182 5896 Fragments arranged by size: 5896 1182 84 69 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7231 NotI GC'GGCC_GC Cuts at: 4678 4678 Size: 7231 NspI r_CATG'y Cuts at: 1132 1229 3226 3631 3697 4468 4589 6134 1132 Size: 97 1997 405 66 771 121 1545 2229 Fragments arranged by size: 2229 1997 1545 771 405 121 97 66 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7231 PciI A'CATG_T Cuts at: 3693 4464 6130 3693 Size: 771 1666 4794 Fragments arranged by size: 4794 1666 771 PflMI CCAn_nnn'nTGG Cuts at: 1108 2677 3511 1108 Size: 1569 834 4828 Fragments arranged by size: 4828 1569 834 PfoI T'CCnGG_A Cuts at: 3736 3736 Size: 7231 PmeI GTTT'AAAC Cuts at: 4535 4535 Size: 7231 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2820 2852 5300 5332 6813 6845 2820 Size: 32 2448 32 1481 32 3206 Fragments arranged by size: 3206 2448 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3730 3730 Size: 7231 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7231 PsiI TTA'TAA Cuts at: 4801 4801 Size: 7231 PspOMI G'GGCC_C Cuts at: 2039 2304 2619 3746 3871 2039 Size: 265 315 1127 125 5399 Fragments arranged by size: 5399 1127 315 265 125 PspXI vC'TCGA_Gb Cuts at: 2769 2769 Size: 7231 PstI C_TGCA'G Cuts at: 830 2156 2232 2790 3825 4114 4479 4583 830 Size: 1326 76 558 1035 289 365 104 3478 Fragments arranged by size: 3478 1326 1035 558 365 289 104 76 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7231 PvuII CAG'CTG Cuts at: 2233 2483 2791 3622 4076 4474 2233 Size: 250 308 831 454 398 4990 Fragments arranged by size: 4990 831 454 398 308 250 SacI G_AGCT'C Cuts at: 3072 3072 Size: 7231 SalI G'TCGA_C Cuts at: 1936 4573 1936 Size: 2637 4594 SapI GCTCTTCn'nnn_ Cuts at: 2151 3884 2151 Size: 1733 5498 SbfI CC_TGCA'GG Cuts at: 4583 4583 Size: 7231 ScaI AGT'ACT Cuts at: 5479 7191 5479 Size: 1712 5519 SexAI A'CCwGG_T Cuts at: 3162 3510 3162 Size: 348 6883 SfcI C'TryA_G Cuts at: 826 1045 2152 2228 2786 2860 3578 3655 3821 Size: 219 1107 76 558 74 718 77 166 Cuts at: 3821 4110 4475 4579 5714 6395 6586 826 Size: 289 365 104 1135 681 191 1471 Fragments arranged by size: 1471 1135 1107 718 681 558 365 289 219 191 166 104 77 76 74 SfiI GGCCn_nnn'nGGCC Cuts at: 2280 2280 Size: 7231 SfoI GGC'GCC Cuts at: 2359 3095 2359 Size: 736 6495 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7231 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7231 SmaI CCC'GGG Cuts at: 1872 2300 3353 4558 1872 Size: 428 1053 1205 4545 Fragments arranged by size: 4545 1205 1053 428 SmlI C'TyrA_G Cuts at: 820 1017 1943 2769 2939 4229 5335 6236 6498 Size: 197 926 826 170 1290 1106 901 262 Cuts at: 6498 820 Size: 1553 Fragments arranged by size: 1553 1290 1106 926 901 826 262 197 170 SnaBI TAC'GTA Cuts at: 493 5022 493 Size: 4529 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7231 SphI G_CATG'C Cuts at: 1132 4589 1132 Size: 3457 3774 SspI AAT'ATT Cuts at: 5 52 5155 5 Size: 47 5103 2081 Fragments arranged by size: 5103 2081 47 StuI AGG'CCT Cuts at: 1517 3983 1517 Size: 2466 4765 StyI C'CwwG_G Cuts at: 513 1065 2004 2133 2897 4521 4670 513 Size: 552 939 129 764 1624 149 3074 Fragments arranged by size: 3074 1624 939 764 552 149 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2560 2685 5258 5275 5428 5613 6058 Size: 1255 980 125 2573 17 153 185 445 Cuts at: 6058 325 Size: 1498 Fragments arranged by size: 2573 1498 1255 980 445 185 153 125 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2969 3624 5477 Size: 274 80 33 51 1443 992 655 1853 Cuts at: 5477 7189 96 Size: 1712 138 Fragments arranged by size: 1853 1712 1443 992 655 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7191 7223 7191 Size: 32 7199 Tth111I GACn'n_nGTC Cuts at: 1316 2631 4022 1316 Size: 1315 1391 4525 Fragments arranged by size: 4525 1391 1315 XbaI T'CTAG_A Cuts at: 4567 4567 Size: 7231 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2523 4342 2011 Size: 512 1819 4900 Fragments arranged by size: 4900 1819 512 XhoI C'TCGA_G Cuts at: 1943 2769 1943 Size: 826 6405 XmaI C'CCGG_G Cuts at: 1870 2298 3351 4556 1870 Size: 428 1053 1205 4545 Fragments arranged by size: 4545 1205 1053 428 XmnI GAAnn'nnTTC Cuts at: 5360 5360 Size: 7231 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5039 276 Size: 53 83 186 936 3505 2468 Fragments arranged by size: 3505 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoRV FalI FspAI NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SrfI SwaI