################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AhdI Alw44I AlwI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AgeI 398 ACCGGT A^CCGGT AocI 498 CCTGAGG CC^T[ACGT]AGG AspHI 25 GAGCAC G[AT]GC[AT]^C AvaI 16 CCCGAG C^[CT]CG[GA]G BalI 193 TGGCCA TGG^CCA BbsI 256 GTCTTC GAAGAC(2/6) BciVI 62 GTATCC GTATCC(6/5) BpmI 1012 CTGGAG CTGGAG(16/14) BpuAI 256 GTCTTC GAAGAC(2/6) BsiHKAI 25 GAGCAC G[AT]GC[AT]^C BsmAI 118 GAGAC GTCTC(1/5) BsmI 682 GCATTC GAATGC(1/-1) BsoBI 16 CCCGAG C^[CT]CG[GA]G BsrBI 568 GAGCGG CCGCTC(-3/-3) BsrFI 398 ACCGGT [GA]^CCGG[CT] BstXI 882 CCAGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 498 CCTGAGG CC^T[ACGT]AGG Cfr10I 398 ACCGGT [GA]^CCGG[CT] DpnII 1023 GATC ^GATC DsaI 1155 CCGTGG C^C[GA][CT]GG EaeI 193 TGGCCA [CT]^GGCC[GA] EarI 43 CTCTTC CTCTTC(1/4) Eco81I 498 CCTGAGG CC^T[ACGT]AGG HaeII 808 AGCGCC [GA]GCGC^[CT] HincII 186 GTTGAC GT[CT]^[GA]AC HindII 186 GTTGAC GT[CT]^[GA]AC Ksp632I 43 CTCTTC CTCTTC(1/4) MaeII 788 ACGT A^CGT MboI 1023 GATC ^GATC MluNI 193 TGGCCA TGG^CCA MscI 193 TGGCCA TGG^CCA NdeII 1023 GATC ^GATC PinAI 398 ACCGGT A^CCGGT SapI 42 GCTCTTC GCTCTTC(1/4) Sau3AI 1023 GATC ^GATC StuI 290 AGGCCT AGG^CCT TfiI 301 GATTC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 1057 CGCG CG^CG 1059 CGCG AfaI 54 GTAC GT^AC 360 GTAC BcnI 687 CCGGG CC^[GC]GG 984 CCGGG BglI 128 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 496 GCCCTGAGGGC BmyI 25 GAGCAC G[AGT]GC[ACT]^C 329 GTGCCC BsaWI 398 ACCGGT [AT]^CCGG[AT] 834 ACCGGA Bsp1286I 25 GAGCAC G[AGT]GC[ACT]^C 329 GTGCCC BspMI 56 ACCTGC ACCTGC(4/8) 795 ACCTGC BstAPI 505 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 702 GCAGAGGCTGC BstUI 1057 CGCG CG^CG 1059 CGCG CfoI 809 GCGC GCG^C 1058 GCGC DraII 12 GGGTCCC [GA]G^G[ACGT]CC[CT] 1067 GGGTCCC EcoNI 516 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 726 CCTGAAAGAGG EcoO109I 12 GGGTCCC [GA]G^G[ACGT]CC[CT] 1067 GGGTCCC EcoT14I 456 CCAAGG C^C[AT][AT]GG 964 CCTTGG FauI 125 CCCGC CCCGC(4/6) 588 GCGGG HgaI 39 GACGC GACGC(5/10) 347 GCGTC HhaI 809 GCGC GCG^C 1058 GCGC HinP1I 809 GCGC G^CGC 1058 GCGC MboII 44 TCTTC GAAGA(8/7) 257 TCTTC MvnI 1057 CGCG CG^CG 1059 CGCG NciI 687 CCGGG CC^[GC]GG 984 CCGGG PflMI 357 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 456 CCAAGGCATGG PpuMI 12 GGGTCCC [GA]G^G[AT]CC[CT] 1067 GGGTCCC PstI 709 CTGCAG CTGCA^G 868 CTGCAG RsaI 54 GTAC GT^AC 360 GTAC SfcI 709 CTGCAG C^T[GA][CT]AG 868 CTGCAG StyI 456 CCAAGG C^C[AT][AT]GG 964 CCTTGG Van91I 357 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 456 CCAAGGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AluI AlwNI AvaII BanI BbvI BfaI BsaJI BseRI BsgI BsiYI BslI BsmFI BsrI BssKI BstEII BstF5I BstNI BstPI Cac8I Cfr13I DdeI EcoO65I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MnlI MslI MspA1I MspI MvaI MwoI NlaIII NlaIV PleI PvuII Sau96I ScrFI SfaNI TseI Tsp45I TspRI