(Circular) (Six-base) MAPSORT of: pFN21AE4389.seq Check: 5506 from: 1 to: 5929 pFN21AE4389 5929 bp With 182 enzymes: SgfI * March 28, 2012 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3739 278 Size: 53 83 186 936 2203 2468 Fragments arranged by size: 2468 2203 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3272 1937 Size: 1335 4594 Acc65I G'GTAC_C Cuts at: 3250 3250 Size: 5929 AclI AA'CG_TT Cuts at: 1563 4056 4429 1563 Size: 2493 373 3063 Fragments arranged by size: 3063 2493 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2408 3992 5376 5644 1632 Size: 776 1584 1384 268 1917 Fragments arranged by size: 1917 1584 1384 776 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5732 AflIII A'CryG_T Cuts at: 1203 4828 5805 1203 Size: 3625 977 1327 Fragments arranged by size: 3625 1327 977 AgeI A'CCGG_T Cuts at: 2403 4760 2403 Size: 2357 3572 AhdI GACnn_n'nnGTC Cuts at: 4658 4658 Size: 5929 AleI CACnn'nnGTG Cuts at: 1166 2249 3110 1166 Size: 1083 861 3985 Fragments arranged by size: 3985 1083 861 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3128 1184 Size: 1944 3985 AlwNI CAG_nnn'CTG Cuts at: 2062 2368 2713 3672 5244 2062 Size: 306 345 959 1572 2747 Fragments arranged by size: 2747 1572 959 345 306 ApaLI G'TGCA_C Cuts at: 3986 5142 3986 Size: 1156 4773 ApoI r'AATT_y Cuts at: 1493 3238 3464 3616 5725 1493 Size: 1745 226 152 2109 1697 Fragments arranged by size: 2109 1745 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4483 160 Size: 872 3451 1606 Fragments arranged by size: 3451 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5929 AvaI C'yCGr_G Cuts at: 1870 1943 2021 3254 1870 Size: 73 78 1233 4545 Fragments arranged by size: 4545 1233 78 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2050 2083 2050 Size: 33 5896 BamHI G'GATC_C Cuts at: 3259 3626 3259 Size: 367 5562 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2167 2204 2254 2333 2836 Size: 325 217 627 380 37 50 79 503 Cuts at: 2836 2883 3250 4705 618 Size: 47 367 1455 1842 Fragments arranged by size: 1842 1455 627 503 380 367 325 217 79 50 47 37 BanII G_rGCy'C Cuts at: 1391 3248 1391 Size: 1857 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2254 928 Size: 971 355 4603 Fragments arranged by size: 4603 971 355 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4120 4154 1903 Size: 34 2183 34 3678 Fragments arranged by size: 3678 2183 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2078 3821 5031 2078 Size: 1743 1210 2976 Fragments arranged by size: 2976 1743 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5929 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2139 2507 4540 Size: 107 122 71 631 605 467 368 2033 Cuts at: 4540 136 Size: 1525 Fragments arranged by size: 2033 1525 631 605 467 368 122 107 71 BglII A'GATC_T Cuts at: 5924 5924 Size: 5929 BlpI GC'TnA_GC Cuts at: 3346 3346 Size: 5929 Bme1580I G_kGCm'C Cuts at: 1790 2338 3990 5146 1790 Size: 548 1652 1156 2573 Fragments arranged by size: 2573 1652 1156 548 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5929 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2616 3213 3660 Size: 416 53 83 399 24 1199 597 447 Cuts at: 3660 4618 442 Size: 958 1753 Fragments arranged by size: 1753 1199 958 597 447 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5929 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3038 4589 1239 Size: 1799 1551 2579 Fragments arranged by size: 2579 1799 1551 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4054 4919 5217 4054 Size: 865 298 4766 Fragments arranged by size: 4766 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4592 882 Size: 617 3093 2219 Fragments arranged by size: 3093 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3720 493 Size: 711 2516 2702 Fragments arranged by size: 2702 2516 711 BsaBI GATnn'nnATC Cuts at: 5923 5923 Size: 5929 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3736 4118 4753 275 Size: 53 83 186 936 2203 382 635 1451 Fragments arranged by size: 2203 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2403 2839 4362 4760 5034 5181 5592 2403 Size: 436 1523 398 274 147 411 2740 Fragments arranged by size: 2740 1523 436 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2091 2121 1178 Size: 30 165 30 688 30 4986 Fragments arranged by size: 4986 688 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2042 2135 2212 2395 2459 2879 1186 Size: 853 3 93 77 183 64 420 4236 Fragments arranged by size: 4236 853 420 183 93 77 64 3 BseYI C'CCAG_C Cuts at: 2137 3076 5132 5628 2137 Size: 939 2056 496 2438 Fragments arranged by size: 2438 2056 939 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2501 2645 3104 1163 Size: 573 765 144 459 3988 Fragments arranged by size: 3988 765 573 459 144 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3379 4140 5168 1540 Size: 275 186 1378 761 1028 2301 Fragments arranged by size: 2301 1378 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 3990 4075 5146 2034 Size: 1956 85 1071 2817 Fragments arranged by size: 2817 1956 1071 85 BsmI GAATG_Cn' Cuts at: 2687 3440 3533 2687 Size: 753 93 5083 Fragments arranged by size: 5083 753 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2338 3248 3990 4075 5146 1391 Size: 399 244 304 910 742 85 1071 2174 Fragments arranged by size: 2174 1071 910 742 399 304 244 85 BspEI T'CCGG_A Cuts at: 5592 5592 Size: 5929 BspHI T'CATG_A Cuts at: 3818 5548 3818 Size: 1730 4199 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2070 2809 3284 844 Size: 1226 739 475 3489 Fragments arranged by size: 3489 1226 739 475 BsrBI CCG'CTC Cuts at: 1394 1823 2575 3816 1394 Size: 429 752 1241 3507 Fragments arranged by size: 3507 1241 752 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4424 4598 65 Size: 1183 3176 174 1396 Fragments arranged by size: 3176 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2403 4573 4760 1700 Size: 110 593 2170 187 2869 Fragments arranged by size: 2869 2170 593 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5929 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5929 BssSI C'ACGA_G Cuts at: 3989 5001 3989 Size: 1012 4917 BstAPI GCAn_nnn'nTGC Cuts at: 794 2516 2713 794 Size: 1722 197 4010 Fragments arranged by size: 4010 1722 197 BstBI TT'CG_AA Cuts at: 4733 4733 Size: 5929 BstEII G'GTnAC_C Cuts at: 1184 2182 2456 2596 2905 1184 Size: 998 274 140 309 4208 Fragments arranged by size: 4208 998 309 274 140 BstXI CCAn_nnnn'nTGG Cuts at: 2894 2894 Size: 5929 BstYI r'GATC_y Cuts at: 1972 3259 3626 4011 4028 5480 5566 5578 5924 Size: 1287 367 385 17 1452 86 12 346 Cuts at: 5924 1972 Size: 1977 Fragments arranged by size: 1977 1452 1287 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2504 2504 Size: 5929 BtgI C'CryG_G Cuts at: 513 1065 3160 513 Size: 552 2095 3282 Fragments arranged by size: 3282 2095 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5190 Fragments arranged by size: 5190 683 56 BtsI GCAGTG_nn' Cuts at: 3445 4239 4259 3445 Size: 794 20 5115 Fragments arranged by size: 5115 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5929 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5894 DraI TTT'AAA Cuts at: 3233 3233 Size: 5929 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4936 809 Size: 507 3620 1802 Fragments arranged by size: 3620 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2198 3376 4265 Size: 54 1425 50 275 18 368 1178 889 Cuts at: 4265 5894 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1178 889 368 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3376 1537 Size: 275 1564 4090 Fragments arranged by size: 4090 1564 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 3859 1353 Size: 701 1805 3423 Fragments arranged by size: 3423 1805 701 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 4516 4890 5036 387 Size: 1064 138 453 2474 374 146 1280 Fragments arranged by size: 2474 1280 1064 453 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2408 3038 3992 4589 5376 5644 1239 Size: 393 776 630 954 597 787 268 1524 Fragments arranged by size: 1524 954 787 776 630 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2525 2735 2525 Size: 210 5719 EcoO109I rG'GnC_Cy Cuts at: 2018 3073 2018 Size: 1055 4874 EcoRI G'AATT_C Cuts at: 3238 3616 3238 Size: 378 5551 FspI TGC'GCA Cuts at: 4435 4435 Size: 5929 HaeII r_GCGC'y Cuts at: 1678 2817 5076 1678 Size: 1139 2259 2531 Fragments arranged by size: 2531 2259 1139 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 4570 4602 4644 Size: 32 135 32 449 32 2517 32 42 Cuts at: 4644 4676 1373 Size: 32 2626 Fragments arranged by size: 2626 2517 449 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2193 3273 3519 669 Size: 988 281 255 1080 246 3079 Fragments arranged by size: 3079 1080 988 281 255 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5929 HpaI GTT'AAC Cuts at: 3519 3519 Size: 5929 KpnI G_GTAC'C Cuts at: 3254 3254 Size: 5929 MfeI C'AATT_G Cuts at: 3528 3528 Size: 5929 MluI A'CGCG_T Cuts at: 5805 5805 Size: 5929 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5043 5227 1305 Size: 3738 184 2007 Fragments arranged by size: 3738 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2200 10 Size: 54 1425 343 368 3739 Fragments arranged by size: 3739 1425 368 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2247 2249 2423 3110 3887 4246 4405 Size: 648 1081 2 174 687 777 359 159 Cuts at: 4405 518 Size: 2042 Fragments arranged by size: 2042 1081 777 687 648 359 174 159 2 MspA1I CmG'CkG Cuts at: 1606 2137 2485 2872 2894 3345 4022 5170 5415 Size: 531 348 387 22 451 677 1148 245 Cuts at: 5415 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 531 451 387 348 245 22 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5377 NdeI CA'TA_TG Cuts at: 387 387 Size: 5929 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5929 NotI GC'GGCC_GC Cuts at: 3376 3376 Size: 5929 NspI r_CATG'y Cuts at: 1132 1229 3287 4832 1132 Size: 97 2058 1545 2229 Fragments arranged by size: 2229 2058 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5929 PciI A'CATG_T Cuts at: 4828 4828 Size: 5929 PflMI CCAn_nnn'nTGG Cuts at: 1108 2368 2467 1108 Size: 1260 99 4570 Fragments arranged by size: 4570 1260 99 PfoI T'CCnGG_A Cuts at: 2691 2691 Size: 5929 PmeI GTTT'AAAC Cuts at: 3233 3233 Size: 5929 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3137 3169 3998 4030 5511 5543 3137 Size: 32 829 32 1481 32 3523 Fragments arranged by size: 3523 1481 829 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2018 3073 2018 Size: 1055 4874 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5929 PsiI TTA'TAA Cuts at: 3499 3499 Size: 5929 PstI C_TGCA'G Cuts at: 830 2718 2877 3281 830 Size: 1888 159 404 3478 Fragments arranged by size: 3478 1888 404 159 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5929 PvuII CAG'CTG Cuts at: 2137 2872 2894 2137 Size: 735 22 5172 Fragments arranged by size: 5172 735 22 SalI G'TCGA_C Cuts at: 1936 3271 1936 Size: 1335 4594 SanDI GG'GwC_CC Cuts at: 2018 3073 2018 Size: 1055 4874 SapI GCTCTTCn'nnn_ Cuts at: 2054 2054 Size: 5929 SbfI CC_TGCA'GG Cuts at: 3281 3281 Size: 5929 ScaI AGT'ACT Cuts at: 4177 5889 4177 Size: 1712 4217 SfcI C'TryA_G Cuts at: 826 1045 2714 2873 3277 4412 5093 5284 826 Size: 219 1669 159 404 1135 681 191 1471 Fragments arranged by size: 1669 1471 1135 681 404 219 191 159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5929 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5929 SmaI CCC'GGG Cuts at: 1872 3256 1872 Size: 1384 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4033 4934 5196 820 Size: 197 926 2090 901 262 1553 Fragments arranged by size: 2090 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3720 493 Size: 3227 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5929 SphI G_CATG'C Cuts at: 1132 3287 1132 Size: 2155 3774 SspI AAT'ATT Cuts at: 5 52 3853 5 Size: 47 3801 2081 Fragments arranged by size: 3801 2081 47 StuI AGG'CCT Cuts at: 1517 2297 1517 Size: 780 5149 StyI C'CwwG_G Cuts at: 513 1065 2461 2969 3368 513 Size: 552 1396 508 399 3074 Fragments arranged by size: 3074 1396 552 508 399 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2049 2578 3109 3956 3973 4126 4311 Size: 1255 469 529 531 847 17 153 185 Cuts at: 4311 4756 325 Size: 445 1498 Fragments arranged by size: 1498 1255 847 531 529 469 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4175 5887 96 Size: 274 80 33 51 1443 2198 1712 138 Fragments arranged by size: 2198 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2091 2123 2333 2365 5889 5921 2091 Size: 32 210 32 3524 32 2099 Fragments arranged by size: 3524 2099 210 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5929 XbaI T'CTAG_A Cuts at: 3265 3265 Size: 5929 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5929 XmaI C'CCGG_G Cuts at: 1870 3254 1870 Size: 1384 4545 XmnI GAAnn'nnTTC Cuts at: 4058 4058 Size: 5929 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3737 276 Size: 53 83 186 936 2203 2468 Fragments arranged by size: 2468 2203 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI