(Linear) (Six-base) MAPSORT of: KIEE4389.seq Check: 2441 from: 1 to: 1224 KIEE4389.orf With 182 enzymes: SgfI * March 22, 2012 17:53 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 403 1224 Size: 403 821 AgeI A'CCGG_T Cuts at: 0 398 1224 Size: 398 826 AleI CACnn'nnGTG Cuts at: 0 244 1105 1224 Size: 244 861 119 Fragments arranged by size: 861 244 119 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1123 1224 Size: 1123 101 AlwNI CAG_nnn'CTG Cuts at: 0 57 363 708 1224 Size: 57 306 345 516 Fragments arranged by size: 516 345 306 57 AvaI C'yCGr_G Cuts at: 0 16 1224 Size: 16 1208 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 45 78 1224 Size: 45 33 1146 Fragments arranged by size: 1146 45 33 BanI G'GyrC_C Cuts at: 0 162 199 249 328 831 878 1224 Size: 162 37 50 79 503 47 346 Fragments arranged by size: 503 346 162 79 50 47 37 BbsI GAAGACnn'nnnn_ Cuts at: 0 249 1224 Size: 249 975 BciVI GTATCCnnnnn_n' Cuts at: 0 73 1224 Size: 73 1151 BglI GCCn_nnn'nGGC Cuts at: 0 134 502 1224 Size: 134 368 722 Fragments arranged by size: 722 368 134 Bme1580I G_kGCm'C Cuts at: 0 333 1224 Size: 333 891 BmrI ACTGGGnnnn_n' Cuts at: 0 611 1208 1224 Size: 611 597 16 Fragments arranged by size: 611 597 16 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1033 1224 Size: 1033 191 BsaWI w'CCGG_w Cuts at: 0 398 834 1224 Size: 398 436 390 Fragments arranged by size: 436 398 390 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 86 116 1224 Size: 86 30 1108 Fragments arranged by size: 1108 86 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 34 37 130 207 390 454 874 1224 Size: 34 3 93 77 183 64 420 350 Fragments arranged by size: 420 350 183 93 77 64 34 3 BseYI C'CCAG_C Cuts at: 0 132 1071 1224 Size: 132 939 153 Fragments arranged by size: 939 153 132 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 496 640 1099 1224 Size: 496 144 459 125 Fragments arranged by size: 496 459 144 125 BsiHKAI G_wGCw'C Cuts at: 0 29 1224 Size: 29 1195 BsmI GAATG_Cn' Cuts at: 0 682 1224 Size: 682 542 Bsp1286I G_dGCh'C Cuts at: 0 29 333 1224 Size: 29 304 891 Fragments arranged by size: 891 304 29 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 65 804 1224 Size: 65 739 420 Fragments arranged by size: 739 420 65 BsrBI CCG'CTC Cuts at: 0 570 1224 Size: 570 654 BsrFI r'CCGG_y Cuts at: 0 398 1224 Size: 398 826 BstAPI GCAn_nnn'nTGC Cuts at: 0 511 708 1224 Size: 511 197 516 Fragments arranged by size: 516 511 197 BstEII G'GTnAC_C Cuts at: 0 177 451 591 900 1224 Size: 177 274 140 309 324 Fragments arranged by size: 324 309 274 177 140 BstXI CCAn_nnnn'nTGG Cuts at: 0 889 1224 Size: 889 335 Bsu36I CC'TnA_GG Cuts at: 0 499 1224 Size: 499 725 BtgI C'CryG_G Cuts at: 0 1155 1224 Size: 1155 69 EaeI y'GGCC_r Cuts at: 0 193 1224 Size: 193 1031 EarI CTCTTCn'nnn_ Cuts at: 0 49 1224 Size: 49 1175 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 37 1224 Size: 37 1187 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 403 1033 1224 Size: 403 630 191 Fragments arranged by size: 630 403 191 EcoNI CCTnn'n_nnAGG Cuts at: 0 520 730 1224 Size: 520 210 494 Fragments arranged by size: 520 494 210 EcoO109I rG'GnC_Cy Cuts at: 0 13 1068 1224 Size: 13 1055 156 Fragments arranged by size: 1055 156 13 HaeII r_GCGC'y Cuts at: 0 812 1224 Size: 812 412 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 16 48 1224 Size: 16 32 1176 Fragments arranged by size: 1176 32 16 HincII GTy'rAC Cuts at: 0 188 1224 Size: 188 1036 MscI TGG'CCA Cuts at: 0 195 1224 Size: 195 1029 MslI CAynn'nnrTG Cuts at: 0 242 244 418 1105 1224 Size: 242 2 174 687 119 Fragments arranged by size: 687 242 174 119 2 MspA1I CmG'CkG Cuts at: 0 132 480 867 889 1224 Size: 132 348 387 22 335 Fragments arranged by size: 387 348 335 132 22 PflMI CCAn_nnn'nTGG Cuts at: 0 363 462 1224 Size: 363 99 762 Fragments arranged by size: 762 363 99 PfoI T'CCnGG_A Cuts at: 0 686 1224 Size: 686 538 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1132 1164 1224 Size: 1132 32 60 Fragments arranged by size: 1132 60 32 PpuMI rG'GwC_Cy Cuts at: 0 13 1068 1224 Size: 13 1055 156 Fragments arranged by size: 1055 156 13 PstI C_TGCA'G Cuts at: 0 713 872 1224 Size: 713 159 352 Fragments arranged by size: 713 352 159 PvuII CAG'CTG Cuts at: 0 132 867 889 1224 Size: 132 735 22 335 Fragments arranged by size: 735 335 132 22 SanDI GG'GwC_CC Cuts at: 0 13 1068 1224 Size: 13 1055 156 Fragments arranged by size: 1055 156 13 SapI GCTCTTCn'nnn_ Cuts at: 0 49 1224 Size: 49 1175 SfcI C'TryA_G Cuts at: 0 709 868 1224 Size: 709 159 356 Fragments arranged by size: 709 356 159 StuI AGG'CCT Cuts at: 0 292 1224 Size: 292 932 StyI C'CwwG_G Cuts at: 0 456 964 1224 Size: 456 508 260 Fragments arranged by size: 508 456 260 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 44 573 1104 1224 Size: 44 529 531 120 Fragments arranged by size: 531 529 120 44 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 86 118 328 360 1224 Size: 86 32 210 32 864 Fragments arranged by size: 864 210 86 32 32 Enzymes that do cut: AcuI AgeI AleI AlfI AlwNI AvaI BaeI BanI BbsI BciVI BglI Bme1580I BmrI BpmI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII BstXI Bsu36I BtgI EaeI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MscI MslI MspA1I PflMI PfoI PpiI PpuMI PstI PvuII SanDI SapI SfcI StuI StyI TaqII TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BamHI BanII BbeI BbvCI BcgI BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI