(Circular) (Six-base) MAPSORT of: pFN21AE3924.seq Check: 3597 from: 1 to: 5743 pFN21AE3924 5743 bp With 182 enzymes: SgfI * March 28, 2012 11:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3553 278 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3086 1937 Size: 1149 4594 Acc65I G'GTAC_C Cuts at: 3064 3064 Size: 5743 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3806 5190 5458 1632 Size: 2174 1384 268 1917 Fragments arranged by size: 2174 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5546 AflIII A'CryG_T Cuts at: 1203 2257 2536 4642 5619 1203 Size: 1054 279 2106 977 1327 Fragments arranged by size: 2106 1327 1054 977 279 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 4472 4472 Size: 5743 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5743 AlwNI CAG_nnn'CTG Cuts at: 2209 2353 2611 3486 5058 2209 Size: 144 258 875 1572 2894 Fragments arranged by size: 2894 1572 875 258 144 ApaI G_GGCC'C Cuts at: 2704 2704 Size: 5743 ApaLI G'TGCA_C Cuts at: 2881 3800 4956 2881 Size: 919 1156 3668 Fragments arranged by size: 3668 1156 919 ApoI r'AATT_y Cuts at: 1493 3052 3278 3430 5539 1493 Size: 1559 226 152 2109 1697 Fragments arranged by size: 2109 1697 1559 226 152 AseI AT'TA_AT Cuts at: 160 1032 4297 160 Size: 872 3265 1606 Fragments arranged by size: 3265 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 3068 1870 Size: 73 1125 4545 Fragments arranged by size: 4545 1125 73 BamHI G'GATC_C Cuts at: 3073 3440 3073 Size: 367 5376 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2096 2647 2907 3064 4519 Size: 325 217 627 309 551 260 157 1455 Cuts at: 4519 618 Size: 1842 Fragments arranged by size: 1842 1455 627 551 325 309 260 217 157 BanII G_rGCy'C Cuts at: 1391 2704 3062 1391 Size: 1313 358 4072 Fragments arranged by size: 4072 1313 358 BbeI G_GCGC'C Cuts at: 2100 2651 2100 Size: 551 5192 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2488 2557 928 Size: 971 589 69 4114 Fragments arranged by size: 4114 971 589 69 BbvCI CC'TCA_GC Cuts at: 2612 2612 Size: 5743 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3934 3968 1903 Size: 34 1997 34 3678 Fragments arranged by size: 3678 1997 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2247 3635 4845 2247 Size: 1388 1210 3145 Fragments arranged by size: 3145 1388 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5743 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2407 4354 136 Size: 107 122 71 631 605 735 1947 1525 Fragments arranged by size: 1947 1525 735 631 605 122 107 71 BglII A'GATC_T Cuts at: 5738 5738 Size: 5743 BlpI GC'TnA_GC Cuts at: 3160 3160 Size: 5743 Bme1580I G_kGCm'C Cuts at: 1790 2265 2704 2885 3804 4960 1790 Size: 475 439 181 919 1156 2573 Fragments arranged by size: 2573 1156 919 475 439 181 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5743 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3474 4432 442 Size: 416 53 83 399 24 2057 958 1753 Fragments arranged by size: 2057 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5743 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2176 2176 Size: 5743 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 2363 2510 2915 4403 1239 Size: 914 210 147 405 1488 2579 Fragments arranged by size: 2579 1488 914 405 210 147 Bpu10I CC'TnA_GC Cuts at: 2419 2612 2419 Size: 193 5550 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3868 4733 5031 3868 Size: 865 298 4580 Fragments arranged by size: 4580 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4406 882 Size: 617 2907 2219 Fragments arranged by size: 2907 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2601 3534 493 Size: 711 1397 933 2702 Fragments arranged by size: 2702 1397 933 711 BsaBI GATnn'nnATC Cuts at: 5737 5737 Size: 5743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2097 2648 3550 3932 Size: 53 83 186 936 564 551 902 382 Cuts at: 3932 4567 275 Size: 635 1451 Fragments arranged by size: 1451 936 902 635 564 551 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4176 4574 4848 4995 5406 4176 Size: 398 274 147 411 4513 Fragments arranged by size: 4513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5518 Fragments arranged by size: 5518 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 2257 2488 2867 2912 1186 Size: 1045 26 231 379 45 4017 Fragments arranged by size: 4017 1045 379 231 45 26 BseYI C'CCAG_C Cuts at: 2722 2943 4946 5442 2722 Size: 221 2003 496 3023 Fragments arranged by size: 3023 2003 496 221 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2087 2726 2801 2986 1163 Size: 573 351 639 75 185 3920 Fragments arranged by size: 3920 639 573 351 185 75 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 3193 3954 4982 1540 Size: 275 186 15 1177 761 1028 2301 Fragments arranged by size: 2301 1177 1028 761 275 186 15 BsiHKAI G_wGCw'C Cuts at: 2885 3804 3889 4960 2885 Size: 919 85 1071 3668 Fragments arranged by size: 3668 1071 919 85 BsmI GAATG_Cn' Cuts at: 3254 3347 3254 Size: 93 5650 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2265 2704 2885 3062 3804 3889 4960 Size: 399 475 439 181 177 742 85 1071 Cuts at: 4960 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 475 439 399 181 177 85 BspEI T'CCGG_A Cuts at: 5406 5406 Size: 5743 BspHI T'CATG_A Cuts at: 3632 5362 3632 Size: 1730 4013 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2325 3098 844 Size: 1481 773 3489 Fragments arranged by size: 3489 1481 773 BsrBI CCG'CTC Cuts at: 1394 1823 3630 1394 Size: 429 1807 3507 Fragments arranged by size: 3507 1807 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4238 4412 65 Size: 1183 2990 174 1396 Fragments arranged by size: 2990 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4387 4574 1700 Size: 110 2577 187 2869 Fragments arranged by size: 2869 2577 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5743 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5743 BssSI C'ACGA_G Cuts at: 3803 4815 3803 Size: 1012 4731 BstAPI GCAn_nnn'nTGC Cuts at: 794 2353 2749 2889 794 Size: 1559 396 140 3648 Fragments arranged by size: 3648 1559 396 140 BstBI TT'CG_AA Cuts at: 4547 4547 Size: 5743 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5743 BstYI r'GATC_y Cuts at: 1972 2620 3073 3440 3825 3842 5294 5380 5392 Size: 648 453 367 385 17 1452 86 12 Cuts at: 5392 5738 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 648 453 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2627 2696 2627 Size: 69 5674 BtgI C'CryG_G Cuts at: 513 1065 2004 2068 2278 2557 2757 2821 513 Size: 552 939 64 210 279 200 64 3435 Fragments arranged by size: 3435 939 552 279 210 200 64 64 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2232 487 Size: 683 56 1006 3998 Fragments arranged by size: 3998 1006 683 56 BtsI GCAGTG_nn' Cuts at: 2735 3259 4053 4073 2735 Size: 524 794 20 4405 Fragments arranged by size: 4405 794 524 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5743 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5708 DraI TTT'AAA Cuts at: 3047 3047 Size: 5743 DraIII CAC_nnn'GTG Cuts at: 2749 2889 2961 2749 Size: 140 72 5531 Fragments arranged by size: 5531 140 72 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4750 809 Size: 507 3434 1802 Fragments arranged by size: 3434 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2451 2577 Size: 54 1425 50 275 18 183 438 126 Cuts at: 2577 2929 3190 4079 5708 8 Size: 352 261 889 1629 43 Fragments arranged by size: 1629 1425 889 438 352 275 261 183 126 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2013 3190 1537 Size: 275 201 1177 4090 Fragments arranged by size: 4090 1177 275 201 EarI CTCTTCn'nnn_ Cuts at: 1353 3673 1353 Size: 2320 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2993 4330 4704 4850 387 Size: 1064 138 1404 1337 374 146 1280 Fragments arranged by size: 1404 1337 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2363 2510 2915 3806 4403 5190 Size: 393 521 210 147 405 891 597 787 Cuts at: 5190 5458 1239 Size: 268 1524 Fragments arranged by size: 1524 891 787 597 521 405 393 268 210 147 EcoNI CCTnn'n_nnAGG Cuts at: 2213 2345 2507 2213 Size: 132 162 5449 Fragments arranged by size: 5449 162 132 EcoO109I rG'GnC_Cy Cuts at: 2329 2512 2632 2692 2700 2841 2329 Size: 183 120 60 8 141 5231 Fragments arranged by size: 5231 183 141 120 60 8 EcoRI G'AATT_C Cuts at: 3052 3430 3052 Size: 378 5365 FspI TGC'GCA Cuts at: 4249 4249 Size: 5743 HaeII r_GCGC'y Cuts at: 1678 2100 2434 2483 2612 2651 4890 1678 Size: 422 334 49 129 39 2239 2531 Fragments arranged by size: 2531 2239 422 334 129 49 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2499 2531 2605 2637 4384 Size: 32 135 32 927 32 74 32 1747 Cuts at: 4384 4416 4458 4490 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1747 927 135 74 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3087 3333 669 Size: 988 281 1149 246 3079 Fragments arranged by size: 3079 1149 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5743 HpaI GTT'AAC Cuts at: 3333 3333 Size: 5743 KasI G'GCGC_C Cuts at: 2096 2647 2096 Size: 551 5192 KpnI G_GTAC'C Cuts at: 3068 3068 Size: 5743 MfeI C'AATT_G Cuts at: 3342 3342 Size: 5743 MluI A'CGCG_T Cuts at: 5619 5619 Size: 5743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4857 5041 1305 Size: 3552 184 2007 Fragments arranged by size: 3552 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2579 10 Size: 54 1425 343 747 3174 Fragments arranged by size: 3174 1425 747 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2237 3701 4060 4219 518 Size: 648 1071 1464 359 159 2042 Fragments arranged by size: 2042 1464 1071 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2070 2530 2770 2788 2823 3159 3836 4984 Size: 464 460 240 18 35 336 677 1148 Cuts at: 4984 5229 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 464 460 336 245 240 35 18 NarI GG'CG_CC Cuts at: 2097 2648 2097 Size: 551 5192 NcoI C'CATG_G Cuts at: 513 1065 2004 2757 513 Size: 552 939 753 3499 Fragments arranged by size: 3499 939 753 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5743 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5743 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 2261 2303 2540 3101 4646 1132 Size: 97 1032 42 237 561 1545 2229 Fragments arranged by size: 2229 1545 1032 561 237 97 42 PasI CC'CwG_GG Cuts at: 1348 2209 1348 Size: 861 4882 PciI A'CATG_T Cuts at: 2257 2536 4642 2257 Size: 279 2106 3358 Fragments arranged by size: 3358 2106 279 PflMI CCAn_nnn'nTGG Cuts at: 1108 2209 1108 Size: 1101 4642 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3812 3844 5325 5357 3812 Size: 32 1481 32 4198 Fragments arranged by size: 4198 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2632 2692 2632 Size: 60 5683 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5743 PsiI TTA'TAA Cuts at: 3313 3313 Size: 5743 PspOMI G'GGCC_C Cuts at: 2700 2700 Size: 5743 PstI C_TGCA'G Cuts at: 830 2358 2769 3095 830 Size: 1528 411 326 3478 Fragments arranged by size: 3478 1528 411 326 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 PvuII CAG'CTG Cuts at: 2770 2770 Size: 5743 SacII CC_GC'GG Cuts at: 2071 2824 2071 Size: 753 4990 SalI G'TCGA_C Cuts at: 1936 3085 1936 Size: 1149 4594 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 2063 3991 5703 2063 Size: 1928 1712 2103 Fragments arranged by size: 2103 1928 1712 SexAI A'CCwGG_T Cuts at: 2745 2745 Size: 5743 SfcI C'TryA_G Cuts at: 826 1045 2354 2713 2765 3091 4226 4907 5098 Size: 219 1309 359 52 326 1135 681 191 Cuts at: 5098 826 Size: 1471 Fragments arranged by size: 1471 1309 1135 681 359 326 219 191 52 SfoI GGC'GCC Cuts at: 2098 2649 2098 Size: 551 5192 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 3070 1872 Size: 1198 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3847 4748 5010 820 Size: 197 926 1904 901 262 1553 Fragments arranged by size: 1904 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 2303 3101 1132 Size: 1171 798 3774 Fragments arranged by size: 3774 1171 798 SspI AAT'ATT Cuts at: 5 52 3667 5 Size: 47 3615 2081 Fragments arranged by size: 3615 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5743 StyI C'CwwG_G Cuts at: 513 1065 2004 2757 2847 3182 513 Size: 552 939 753 90 335 3074 Fragments arranged by size: 3074 939 753 552 335 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2925 3770 3787 3940 4125 4570 325 Size: 1255 1345 845 17 153 185 445 1498 Fragments arranged by size: 1498 1345 1255 845 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2061 3989 5701 Size: 274 80 33 51 1443 84 1928 1712 Cuts at: 5701 96 Size: 138 Fragments arranged by size: 1928 1712 1443 274 138 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5703 5735 5703 Size: 32 5711 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5743 XbaI T'CTAG_A Cuts at: 3079 3079 Size: 5743 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5743 XmaI C'CCGG_G Cuts at: 1870 3068 1870 Size: 1198 4545 XmnI GAAnn'nnTTC Cuts at: 3872 3872 Size: 5743 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3551 276 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SapI SfiI SrfI SwaI XcmI