(Circular) (Six-base) MAPSORT of: pFN21AB0404.seq Check: 838 from: 1 to: 6541 pFN21AB0404 6541 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2284 2885 2284 Size: 601 5940 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4351 278 Size: 53 83 186 936 2815 2468 Fragments arranged by size: 2815 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3884 1937 Size: 1947 4594 Acc65I G'GTAC_C Cuts at: 3862 3862 Size: 6541 AclI AA'CG_TT Cuts at: 1563 4668 5041 1563 Size: 3105 373 3063 Fragments arranged by size: 3105 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2360 3464 3660 3669 3750 4604 5988 6256 Size: 728 1104 196 9 81 854 1384 268 Cuts at: 6256 1632 Size: 1917 Fragments arranged by size: 1917 1384 1104 854 728 268 196 81 9 AfeI AGC'GCT Cuts at: 2213 2213 Size: 6541 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6344 AflIII A'CryG_T Cuts at: 1203 5440 6417 1203 Size: 4237 977 1327 Fragments arranged by size: 4237 1327 977 AgeI A'CCGG_T Cuts at: 5372 5372 Size: 6541 AhdI GACnn_n'nnGTC Cuts at: 5270 5270 Size: 6541 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6541 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6541 AlwNI CAG_nnn'CTG Cuts at: 3035 3301 4284 5856 3035 Size: 266 983 1572 3720 Fragments arranged by size: 3720 1572 983 266 ApaLI G'TGCA_C Cuts at: 3657 4598 5754 3657 Size: 941 1156 4444 Fragments arranged by size: 4444 1156 941 ApoI r'AATT_y Cuts at: 1493 2366 3308 3830 3850 4076 4228 6337 1493 Size: 873 942 522 20 226 152 2109 1697 Fragments arranged by size: 2109 1697 942 873 522 226 152 20 AseI AT'TA_AT Cuts at: 160 1032 5095 160 Size: 872 4063 1606 Fragments arranged by size: 4063 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6541 AvaI C'yCGr_G Cuts at: 1870 1943 2565 3866 1870 Size: 73 622 1301 4545 Fragments arranged by size: 4545 1301 622 73 BamHI G'GATC_C Cuts at: 2295 3330 3871 4238 2295 Size: 1035 541 367 4598 Fragments arranged by size: 4598 1035 541 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2878 2986 3718 3862 5317 Size: 325 217 627 1091 108 732 144 1455 Cuts at: 5317 618 Size: 1842 Fragments arranged by size: 1842 1455 1091 732 627 325 217 144 108 BanII G_rGCy'C Cuts at: 1391 2315 2643 2906 3797 3860 1391 Size: 924 328 263 891 63 4072 Fragments arranged by size: 4072 924 891 328 263 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2348 928 Size: 971 449 5121 Fragments arranged by size: 5121 971 449 BbvCI CC'TCA_GC Cuts at: 2994 2994 Size: 6541 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4732 4766 1903 Size: 34 2795 34 3678 Fragments arranged by size: 3678 2795 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4433 5643 4433 Size: 1210 5331 BclI T'GATC_A Cuts at: 1548 1548 Size: 6541 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5152 136 Size: 107 122 71 631 605 337 3143 1525 Fragments arranged by size: 3143 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2836 3699 6536 2836 Size: 863 2837 2841 Fragments arranged by size: 2841 2837 863 BlpI GC'TnA_GC Cuts at: 3958 3958 Size: 6541 Bme1580I G_kGCm'C Cuts at: 1790 3661 3815 4602 5758 1790 Size: 1871 154 787 1156 2573 Fragments arranged by size: 2573 1871 1156 787 154 BmgBI CAC'GTC Cuts at: 1318 2182 1318 Size: 864 5677 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2473 2709 3311 Size: 416 53 83 399 24 1056 236 602 Cuts at: 3311 4272 5230 442 Size: 961 958 1753 Fragments arranged by size: 1753 1056 961 958 602 416 399 236 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6541 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2343 3374 2343 Size: 1031 5510 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2256 3536 3783 3788 5201 1239 Size: 884 133 1280 247 5 1413 2579 Fragments arranged by size: 2579 1413 1280 884 247 133 5 Bpu10I CC'TnA_GC Cuts at: 2994 2994 Size: 6541 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3677 4666 5531 5829 3677 Size: 989 865 298 4389 Fragments arranged by size: 4389 989 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2855 3345 5204 882 Size: 617 1356 490 1859 2219 Fragments arranged by size: 2219 1859 1356 617 490 BsaAI yAC'GTr Cuts at: 493 1204 4332 493 Size: 711 3128 2702 Fragments arranged by size: 3128 2702 711 BsaBI GATnn'nnATC Cuts at: 2135 6535 2135 Size: 4400 2141 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2380 4348 4730 5365 Size: 53 83 186 936 847 1968 382 635 Cuts at: 5365 275 Size: 1451 Fragments arranged by size: 1968 1451 936 847 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4974 5372 5646 5793 6204 4974 Size: 398 274 147 411 5311 Fragments arranged by size: 5311 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6316 Fragments arranged by size: 6316 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 2185 2279 2667 2690 2777 2840 2996 Size: 934 65 94 388 23 87 63 156 Cuts at: 2996 3011 3128 3266 1186 Size: 15 117 138 4461 Fragments arranged by size: 4461 934 388 156 138 117 94 87 65 63 23 15 BseYI C'CCAG_C Cuts at: 5744 6240 5744 Size: 496 6045 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2622 2706 3086 1163 Size: 573 886 84 380 4618 Fragments arranged by size: 4618 886 573 380 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2629 3991 4752 5780 1540 Size: 275 186 628 1362 761 1028 2301 Fragments arranged by size: 2301 1362 1028 761 628 275 186 BsiHKAI G_wGCw'C Cuts at: 3043 3661 4602 4687 5758 3043 Size: 618 941 85 1071 3826 Fragments arranged by size: 3826 1071 941 618 85 BsmI GAATG_Cn' Cuts at: 4052 4145 4052 Size: 93 6448 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2315 2643 2906 3043 3661 3797 3815 Size: 399 525 328 263 137 618 136 18 Cuts at: 3815 3860 4602 4687 5758 1391 Size: 45 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 618 525 399 328 263 137 136 85 45 18 BspEI T'CCGG_A Cuts at: 6204 6204 Size: 6541 BspHI T'CATG_A Cuts at: 4430 6160 4430 Size: 1730 4811 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2284 2594 2885 3058 3896 844 Size: 1440 310 291 173 838 3489 Fragments arranged by size: 3489 1440 838 310 291 173 BsrBI CCG'CTC Cuts at: 1394 1823 3388 3460 4428 1394 Size: 429 1565 72 968 3507 Fragments arranged by size: 3507 1565 968 429 72 BsrDI GCAATG_nn' Cuts at: 65 1248 2539 3477 5036 5210 65 Size: 1183 1291 938 1559 174 1396 Fragments arranged by size: 1559 1396 1291 1183 938 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2078 2989 3354 5185 5372 1700 Size: 110 268 911 365 1831 187 2869 Fragments arranged by size: 2869 1831 911 365 268 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6541 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6541 BssSI C'ACGA_G Cuts at: 3682 4601 5613 3682 Size: 919 1012 4610 Fragments arranged by size: 4610 1012 919 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6541 BstBI TT'CG_AA Cuts at: 3082 5345 3082 Size: 2263 4278 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6541 BstXI CCAn_nnnn'nTGG Cuts at: 2291 2528 2291 Size: 237 6304 BstYI r'GATC_y Cuts at: 1972 2136 2295 2836 3330 3699 3871 4238 4623 Size: 164 159 541 494 369 172 367 385 Cuts at: 4623 4640 6092 6178 6190 6536 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 541 494 385 369 367 346 172 164 159 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2760 3015 2760 Size: 255 6286 BtgI C'CryG_G Cuts at: 513 1065 2004 3632 513 Size: 552 939 1628 3422 Fragments arranged by size: 3422 1628 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5802 Fragments arranged by size: 5802 683 56 BtsI GCAGTG_nn' Cuts at: 4057 4851 4871 4057 Size: 794 20 5727 Fragments arranged by size: 5727 794 20 ClaI AT'CG_AT Cuts at: 1554 2625 1554 Size: 1071 5470 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6506 DraI TTT'AAA Cuts at: 3845 3845 Size: 6541 DraIII CAC_nnn'GTG Cuts at: 2717 2717 Size: 6541 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5548 809 Size: 507 4232 1802 Fragments arranged by size: 4232 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3988 4877 6506 Size: 54 1425 50 275 18 2158 889 1629 Cuts at: 6506 8 Size: 43 Fragments arranged by size: 2158 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3988 1537 Size: 275 2176 4090 Fragments arranged by size: 4090 2176 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3243 3645 3726 4471 1353 Size: 1890 402 81 745 3423 Fragments arranged by size: 3423 1890 745 402 81 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 3617 5128 5502 5648 387 Size: 1064 138 438 1590 1511 374 146 1280 Fragments arranged by size: 1590 1511 1280 1064 438 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2256 2360 3464 3536 3660 3669 Size: 393 491 133 104 1104 72 124 9 Cuts at: 3669 3750 3783 3788 4604 5201 5988 6256 1239 Size: 81 33 5 816 597 787 268 1524 Fragments arranged by size: 1524 1104 816 787 597 491 393 268 133 124 104 81 72 33 9 5 EcoO109I rG'GnC_Cy Cuts at: 2288 2562 2843 2968 3004 3820 2288 Size: 274 281 125 36 816 5009 Fragments arranged by size: 5009 816 281 274 125 36 EcoRI G'AATT_C Cuts at: 2366 3850 4228 2366 Size: 1484 378 4679 Fragments arranged by size: 4679 1484 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3718 3718 Size: 6541 FspI TGC'GCA Cuts at: 5047 5047 Size: 6541 HaeII r_GCGC'y Cuts at: 1678 2215 5688 1678 Size: 537 3473 2531 Fragments arranged by size: 3473 2531 537 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2370 2402 2630 2662 2802 Size: 32 135 32 798 32 228 32 140 Cuts at: 2802 2834 2852 2884 5182 5214 5256 5288 1373 Size: 32 18 32 2298 32 42 32 2626 Fragments arranged by size: 2626 2298 798 228 140 135 42 32 32 32 32 32 32 32 32 18 HincII GTy'rAC Cuts at: 669 1657 1938 3885 4131 669 Size: 988 281 1947 246 3079 Fragments arranged by size: 3079 1947 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6541 HpaI GTT'AAC Cuts at: 4131 4131 Size: 6541 KpnI G_GTAC'C Cuts at: 3866 3866 Size: 6541 MfeI C'AATT_G Cuts at: 4140 4140 Size: 6541 MluI A'CGCG_T Cuts at: 6417 6417 Size: 6541 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5655 5839 1305 Size: 4350 184 2007 Fragments arranged by size: 4350 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4719 Fragments arranged by size: 4719 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4499 4858 5017 518 Size: 648 3333 359 159 2042 Fragments arranged by size: 3333 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2234 3754 3957 4634 5782 6027 1606 Size: 628 1520 203 677 1148 245 2120 Fragments arranged by size: 2120 1520 1148 677 628 245 203 NaeI GCC'GGC Cuts at: 2991 2991 Size: 6541 NcoI C'CATG_G Cuts at: 513 1065 2004 3632 513 Size: 552 939 1628 3422 Fragments arranged by size: 3422 1628 939 552 NdeI CA'TA_TG Cuts at: 387 2362 387 Size: 1975 4566 NgoMIV G'CCGG_C Cuts at: 2989 2989 Size: 6541 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6541 NotI GC'GGCC_GC Cuts at: 3988 3988 Size: 6541 NspI r_CATG'y Cuts at: 1132 1229 3899 5444 1132 Size: 97 2670 1545 2229 Fragments arranged by size: 2670 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6541 PciI A'CATG_T Cuts at: 5440 5440 Size: 6541 PflMI CCAn_nnn'nTGG Cuts at: 1108 2890 1108 Size: 1782 4759 PfoI T'CCnGG_A Cuts at: 3408 3408 Size: 6541 PmeI GTTT'AAAC Cuts at: 3845 3845 Size: 6541 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2567 2599 4610 4642 6123 6155 2567 Size: 32 2011 32 1481 32 2953 Fragments arranged by size: 2953 2011 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3004 3820 3004 Size: 816 5725 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6541 PsiI TTA'TAA Cuts at: 4111 4111 Size: 6541 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3282 3314 3282 Size: 32 6509 PstI C_TGCA'G Cuts at: 830 3184 3893 830 Size: 2354 709 3478 Fragments arranged by size: 3478 2354 709 PvuI CG_AT'CG Cuts at: 2001 2629 2001 Size: 628 5913 PvuII CAG'CTG Cuts at: 2234 2234 Size: 6541 SalI G'TCGA_C Cuts at: 1936 3883 1936 Size: 1947 4594 SbfI CC_TGCA'GG Cuts at: 3893 3893 Size: 6541 ScaI AGT'ACT Cuts at: 4789 6501 4789 Size: 1712 4829 SfcI C'TryA_G Cuts at: 826 1045 2753 3180 3600 3889 5024 5705 5896 Size: 219 1708 427 420 289 1135 681 191 Cuts at: 5896 826 Size: 1471 Fragments arranged by size: 1708 1471 1135 681 427 420 289 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6541 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6541 SmaI CCC'GGG Cuts at: 1872 2567 3868 1872 Size: 695 1301 4545 Fragments arranged by size: 4545 1301 695 SmlI C'TyrA_G Cuts at: 820 1017 1943 3692 4645 5546 5808 820 Size: 197 926 1749 953 901 262 1553 Fragments arranged by size: 1749 1553 953 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4332 493 Size: 3839 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6541 SphI G_CATG'C Cuts at: 1132 3899 1132 Size: 2767 3774 SspI AAT'ATT Cuts at: 5 52 4465 5 Size: 47 4413 2081 Fragments arranged by size: 4413 2081 47 StuI AGG'CCT Cuts at: 1517 2486 1517 Size: 969 5572 StyI C'CwwG_G Cuts at: 513 1065 2004 2284 2481 2910 3632 3980 513 Size: 552 939 280 197 429 722 348 3074 Fragments arranged by size: 3074 939 722 552 429 348 280 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2728 3363 4568 4585 4738 4923 5368 Size: 1255 1148 635 1205 17 153 185 445 Cuts at: 5368 325 Size: 1498 Fragments arranged by size: 1498 1255 1205 1148 635 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2202 2766 4787 Size: 274 80 33 51 1443 225 564 2021 Cuts at: 4787 6499 96 Size: 1712 138 Fragments arranged by size: 2021 1712 1443 564 274 225 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6501 6533 6501 Size: 32 6509 Tth111I GACn'n_nGTC Cuts at: 1316 2866 1316 Size: 1550 4991 XbaI T'CTAG_A Cuts at: 3877 3877 Size: 6541 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2872 2872 Size: 6541 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6541 XmaI C'CCGG_G Cuts at: 1870 2565 3866 1870 Size: 695 1301 4545 Fragments arranged by size: 4545 1301 695 XmnI GAAnn'nnTTC Cuts at: 4670 4670 Size: 6541 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4349 276 Size: 53 83 186 936 2815 2468 Fragments arranged by size: 2815 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI