################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI AlwNI ApoI AseI AsnI Asp700I Asp718I AvrII BamHI BbrPI BbvCI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstF5I BstZ17I CelII ClaI CpoI DraI DraIII EcoNI EcoRI EcoRV EcoT22I FbaI FokI HindIII HpaI KpnI MaeI MamI MfeI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SbfI SexAI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 1234 GACGTC GACGT^C AccI 517 GTCGAC GT^[AC][GT]AC AhdI 349 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 400 GTGCAC G^TGCAC AlwI 33 GATCC GGATC(4/5) AocI 418 CCTCAGG CC^T[ACGT]AGG ApaI 703 GGGCCC GGGCC^C ApaLI 400 GTGCAC G^TGCAC AscI 609 GGCGCGCC GG^CGCGCC AspEI 349 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 372 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 616 CCCGAG C^[CT]CG[GA]G AviII 185 TGCGCA TGC^GCA BalI 1382 TGGCCA TGG^CCA BbsI 1237 GTCTTC GAAGAC(2/6) BcgI 753 CGAGCTCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 131 AGATCT A^GATCT BpuAI 1237 GTCTTC GAAGAC(2/6) BseRI 859 GAGGAG GAGGAG(10/8) BsmI 990 GAATGC GAATGC(1/-1) BsoBI 616 CCCGAG C^[CT]CG[GA]G BspHI 1130 TCATGA T^CATGA BspMI 235 ACCTGC ACCTGC(4/8) BstEII 1320 GGTCACC G^GT[ACGT]ACC BstPI 1320 GGTCACC G^GT[ACGT]ACC Bsu36I 418 CCTCAGG CC^T[ACGT]AGG DraII 702 GGGGCCC [GA]G^G[ACGT]CC[CT] DrdI 1312 GACTCCGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 349 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1123 CTCTTC CTCTTC(1/4) Eco81I 418 CCTCAGG CC^T[ACGT]AGG EcoO109I 702 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoO65I 1320 GGTCACC G^GT[ACGT]ACC EcoT14I 737 CCAAGG C^C[AT][AT]GG FseI 473 GGCCGGCC GGCCGG^CC FspI 185 TGCGCA TGC^GCA Ksp632I 1123 CTCTTC CTCTTC(1/4) MluI 101 ACGCGT A^CGCGT MluNI 1382 TGGCCA TGG^CCA MscI 1382 TGGCCA TGG^CCA PflFI 372 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 828 CCAGGGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 289 GACGACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1130 TCATGA T^CATGA SalI 517 GTCGAC G^TCGAC SapI 1122 GCTCTTC GCTCTTC(1/4) ScaI 938 AGTACT AGT^ACT SfaNI 1031 GCATC GCATC(5/9) SgrAI 322 CACCGGCG C[GA]^CCGG[CT]G SmlI 718 CTCAAG C^T[CT][GA]AG StuI 1436 AGGCCT AGG^CCT StyI 737 CCAAGG C^C[AT][AT]GG Tth111I 372 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 828 CCAGGGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AflIII 101 ACGCGT A^C[GA][CT]GT 815 ACGTGT Aor51HI 449 AGCGCT AGC^GCT 551 AGCGCT BcnI 253 CCCGG CC^[GC]GG 959 CCGGG BglI 191 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 696 GCCGTCGGGGC BsaI 862 GAGACC GGTCTC(1/5) 874 GAGACC BsrBI 648 CCGCTC CCGCTC(-3/-3) 1221 CCGCTC BssHII 467 GCGCGC G^CGCGC 610 GCGCGC BstXI 734 CCACCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1385 CCATCAAGGTGG BstYI 32 AGATCC [GA]^GATC[CT] 131 AGATCT Eco47III 449 AGCGCT AGC^GCT 551 AGCGCT HincII 424 GTCAAC GT[CT]^[GA]AC 517 GTCGAC HindII 424 GTCAAC GT[CT]^[GA]AC 517 GTCGAC KspI 1164 CCGCGG CCGC^GG 1316 CCGCGG MflI 32 AGATCC [GA]^GATC[CT] 131 AGATCT NaeI 474 GCCGGC GCC^GGC 621 GCCGGC NciI 253 CCCGG CC^[GC]GG 959 CCGGG NgoMIV 474 GCCGGC G^CCGGC 621 GCCGGC PstI 307 CTGCAG CTGCA^G 793 CTGCAG SacI 754 GAGCTC GAGCT^C 1120 GAGCTC SacII 1164 CCGCGG CCGC^GG 1316 CCGCGG XhoII 32 AGATCC [GA]^GATC[CT] 131 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AspHI AvaII BanI BanII BbeI BbvI BmyI BpmI BsaHI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfcI SfoI TaqI TseI Tsp45I TspRI TthHB8I