(Circular) (Six-base) MAPSORT of: pFN21AE4474.seq Check: 5198 from: 1 to: 6184 pFN21AE4474 6184 bp With 182 enzymes: SgfI * February 29, 2012 12:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3243 3994 278 Size: 53 83 186 936 1707 751 2468 Fragments arranged by size: 2468 1707 936 751 186 83 53 AccI GT'mk_AC Cuts at: 1937 2523 3527 1937 Size: 586 1004 4594 Fragments arranged by size: 4594 1004 586 Acc65I G'GTAC_C Cuts at: 3505 3505 Size: 6184 AclI AA'CG_TT Cuts at: 1563 4311 4684 1563 Size: 2748 373 3063 Fragments arranged by size: 3063 2748 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2144 3434 3458 4247 5631 5899 1632 Size: 512 1290 24 789 1384 268 1917 Fragments arranged by size: 1917 1384 1290 789 512 268 24 AfeI AGC'GCT Cuts at: 2456 2558 2456 Size: 102 6082 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5987 AflIII A'CryG_T Cuts at: 1203 2106 2820 5083 6060 1203 Size: 903 714 2263 977 1327 Fragments arranged by size: 2263 1327 977 903 714 AgeI A'CCGG_T Cuts at: 5015 5015 Size: 6184 AhdI GACnn_n'nnGTC Cuts at: 2359 4913 2359 Size: 2554 3630 AleI CACnn'nnGTG Cuts at: 1166 2744 3347 1166 Size: 1578 603 4003 Fragments arranged by size: 4003 1578 603 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6184 AlwNI CAG_nnn'CTG Cuts at: 3927 5499 3927 Size: 1572 4612 ApaI G_GGCC'C Cuts at: 2712 2712 Size: 6184 ApaLI G'TGCA_C Cuts at: 2405 4241 5397 2405 Size: 1836 1156 3192 Fragments arranged by size: 3192 1836 1156 ApoI r'AATT_y Cuts at: 1493 3493 3719 3871 5980 1493 Size: 2000 226 152 2109 1697 Fragments arranged by size: 2109 2000 1697 226 152 AscI GG'CGCG_CC Cuts at: 2615 2615 Size: 6184 AseI AT'TA_AT Cuts at: 160 1032 4738 160 Size: 872 3706 1606 Fragments arranged by size: 3706 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 AvaI C'yCGr_G Cuts at: 1870 1943 2621 3509 1870 Size: 73 678 888 4545 Fragments arranged by size: 4545 888 678 73 BamHI G'GATC_C Cuts at: 3514 3881 3514 Size: 367 5817 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2254 2345 2604 2698 3209 Size: 325 217 627 467 91 259 94 511 Cuts at: 3209 3266 3505 4960 618 Size: 57 239 1455 1842 Fragments arranged by size: 1842 1455 627 511 467 325 259 239 217 94 91 57 BanII G_rGCy'C Cuts at: 1391 2712 2763 3129 3503 1391 Size: 1321 51 366 374 4072 Fragments arranged by size: 4072 1321 374 366 51 BbeI G_GCGC'C Cuts at: 2608 2702 3270 2608 Size: 94 568 5522 Fragments arranged by size: 5522 568 94 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3235 928 Size: 971 1336 3877 Fragments arranged by size: 3877 1336 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2747 2781 4375 4409 1903 Size: 34 810 34 1594 34 3678 Fragments arranged by size: 3678 1594 810 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4076 5286 4076 Size: 1210 4974 BclI T'GATC_A Cuts at: 1548 1548 Size: 6184 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2202 2707 4795 Size: 107 122 71 631 605 530 505 2088 Cuts at: 4795 136 Size: 1525 Fragments arranged by size: 2088 1525 631 605 530 505 122 107 71 BglII A'GATC_T Cuts at: 2136 6179 2136 Size: 4043 2141 BlpI GC'TnA_GC Cuts at: 3601 3601 Size: 6184 Bme1580I G_kGCm'C Cuts at: 1790 2259 2409 2514 2712 3212 4245 5401 1790 Size: 469 150 105 198 500 1033 1156 2573 Fragments arranged by size: 2573 1156 1033 500 469 198 150 105 BmgBI CAC'GTC Cuts at: 1318 2509 1318 Size: 1191 4993 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3915 4873 442 Size: 416 53 83 399 24 2498 958 1753 Fragments arranged by size: 2498 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6184 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2671 2671 Size: 6184 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 2666 2792 2912 2971 3214 3272 4844 Size: 929 498 126 120 59 243 58 1572 Cuts at: 4844 1239 Size: 2579 Fragments arranged by size: 2579 1572 929 498 243 126 120 59 58 Bpu10I CC'TnA_GC Cuts at: 2468 2468 Size: 6184 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2708 4309 5174 5472 2708 Size: 1601 865 298 3420 Fragments arranged by size: 3420 1601 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2861 2873 4847 882 Size: 617 1362 12 1974 2219 Fragments arranged by size: 2219 1974 1362 617 12 BsaAI yAC'GTr Cuts at: 493 1204 3975 493 Size: 711 2771 2702 Fragments arranged by size: 2771 2702 711 BsaBI GATnn'nnATC Cuts at: 6178 6178 Size: 6184 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2520 2605 2699 3050 Size: 53 83 186 936 987 85 94 351 Cuts at: 3050 3219 3240 3267 3432 3991 4373 5008 275 Size: 169 21 27 165 559 382 635 1451 Fragments arranged by size: 1451 987 936 635 559 382 351 186 169 165 94 85 83 53 27 21 BsaWI w'CCGG_w Cuts at: 4617 5015 5289 5436 5847 4617 Size: 398 274 147 411 4954 Fragments arranged by size: 4954 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5959 Fragments arranged by size: 5959 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2879 1186 Size: 1693 4491 BseYI C'CCAG_C Cuts at: 2711 5387 5883 2711 Size: 2676 496 3012 Fragments arranged by size: 3012 2676 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2054 2588 2993 1163 Size: 573 318 534 405 4354 Fragments arranged by size: 4354 573 534 405 318 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2082 2181 2302 2480 2484 2902 Size: 275 186 81 99 121 178 4 418 Cuts at: 2902 3634 4395 5423 1540 Size: 732 761 1028 2301 Fragments arranged by size: 2301 1028 761 732 418 275 186 178 121 99 81 4 BsiHKAI G_wGCw'C Cuts at: 2409 2763 3129 4245 4330 5401 2409 Size: 354 366 1116 85 1071 3192 Fragments arranged by size: 3192 1116 1071 366 354 85 BsmI GAATG_Cn' Cuts at: 3001 3695 3788 3001 Size: 694 93 5397 Fragments arranged by size: 5397 694 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2259 2409 2514 2712 2763 3129 3212 Size: 399 469 150 105 198 51 366 83 Cuts at: 3212 3503 4245 4330 5401 1391 Size: 291 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 469 399 366 291 198 150 105 85 83 51 BspEI T'CCGG_A Cuts at: 5847 5847 Size: 6184 BspHI T'CATG_A Cuts at: 3135 4073 5803 3135 Size: 938 1730 3516 Fragments arranged by size: 3516 1730 938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2249 3539 844 Size: 1405 1290 3489 Fragments arranged by size: 3489 1405 1290 BsrBI CCG'CTC Cuts at: 1394 1823 2655 3228 4071 1394 Size: 429 832 573 843 3507 Fragments arranged by size: 3507 843 832 573 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4679 4853 65 Size: 1183 3431 174 1396 Fragments arranged by size: 3431 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2328 2479 2626 3030 4828 5015 1700 Size: 110 518 151 147 404 1798 187 2869 Fragments arranged by size: 2869 1798 518 404 187 151 147 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6184 BssHII G'CGCG_C Cuts at: 1925 2472 2615 1925 Size: 547 143 5494 Fragments arranged by size: 5494 547 143 BssSI C'ACGA_G Cuts at: 4244 5256 4244 Size: 1012 5172 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6184 BstBI TT'CG_AA Cuts at: 4988 4988 Size: 6184 BstEII G'GTnAC_C Cuts at: 1184 3325 1184 Size: 2141 4043 BstXI CCAn_nnnn'nTGG Cuts at: 2746 3397 2746 Size: 651 5533 BstYI r'GATC_y Cuts at: 1972 2037 2136 3514 3881 4266 4283 5735 5821 Size: 65 99 1378 367 385 17 1452 86 Cuts at: 5821 5833 6179 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1378 385 367 346 99 86 65 17 12 Bsu36I CC'TnA_GG Cuts at: 2424 2424 Size: 6184 BtgI C'CryG_G Cuts at: 513 1065 2496 3169 3321 3456 513 Size: 552 1431 673 152 135 3241 Fragments arranged by size: 3241 1431 673 552 152 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2395 487 Size: 683 56 1169 4276 Fragments arranged by size: 4276 1169 683 56 BtsI GCAGTG_nn' Cuts at: 3319 3700 4494 4514 3319 Size: 381 794 20 4989 Fragments arranged by size: 4989 794 381 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6184 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6149 DraI TTT'AAA Cuts at: 3488 3488 Size: 6184 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3323 5191 809 Size: 507 2007 1868 1802 Fragments arranged by size: 2007 1868 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2178 2477 Size: 54 1425 50 275 18 249 99 299 Cuts at: 2477 2481 2628 2805 2958 3093 3387 3631 4520 Size: 4 147 177 153 135 294 244 889 Cuts at: 4520 6149 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 299 294 275 249 244 177 153 147 135 99 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2079 2178 2477 2481 3631 1537 Size: 275 267 99 299 4 1150 4090 Fragments arranged by size: 4090 1150 299 275 267 99 4 EarI CTCTTCn'nnn_ Cuts at: 1353 3134 4114 1353 Size: 1781 980 3423 Fragments arranged by size: 3423 1781 980 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2101 2234 3464 4771 5145 5291 Size: 1064 138 512 133 1230 1307 374 146 Cuts at: 5291 387 Size: 1280 Fragments arranged by size: 1307 1280 1230 1064 512 374 146 138 133 EcoICRI GAG'CTC Cuts at: 2761 3127 2761 Size: 366 5818 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2168 2666 2792 2912 2971 3214 Size: 393 512 24 498 126 120 59 243 Cuts at: 3214 3272 3434 3458 4247 4844 5631 5899 1239 Size: 58 162 24 789 597 787 268 1524 Fragments arranged by size: 1524 789 787 597 512 498 393 268 243 162 126 120 59 58 24 24 EcoO109I rG'GnC_Cy Cuts at: 2708 2708 Size: 6184 EcoRI G'AATT_C Cuts at: 3493 3871 3493 Size: 378 5806 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2686 2938 2686 Size: 252 5932 FseI GG_CCGG'CC Cuts at: 2483 2483 Size: 6184 FspI TGC'GCA Cuts at: 2192 4690 2192 Size: 2498 3686 HaeII r_GCGC'y Cuts at: 1678 2335 2458 2560 2608 2702 3270 3409 5331 Size: 657 123 102 48 94 568 139 1922 Cuts at: 5331 1678 Size: 2531 Fragments arranged by size: 2531 1922 657 568 139 123 102 94 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 3452 3484 4825 Size: 32 135 32 773 32 1075 32 1341 Cuts at: 4825 4857 4899 4931 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1341 1075 773 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2431 2524 3528 3774 669 Size: 988 281 493 93 1004 246 3079 Fragments arranged by size: 3079 1004 988 493 281 246 93 HindIII A'AGCT_T Cuts at: 748 748 Size: 6184 HpaI GTT'AAC Cuts at: 3774 3774 Size: 6184 KasI G'GCGC_C Cuts at: 2604 2698 3266 2604 Size: 94 568 5522 Fragments arranged by size: 5522 568 94 KpnI G_GTAC'C Cuts at: 3509 3509 Size: 6184 MfeI C'AATT_G Cuts at: 3783 3783 Size: 6184 MluI A'CGCG_T Cuts at: 2106 6060 2106 Size: 3954 2230 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5298 5482 1305 Size: 3993 184 2007 Fragments arranged by size: 3993 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3389 10 Size: 54 1425 343 1557 2805 Fragments arranged by size: 2805 1557 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2414 2744 3161 3347 3395 4142 4501 Size: 648 1248 330 417 186 48 747 359 Cuts at: 4501 4660 518 Size: 159 2042 Fragments arranged by size: 2042 1248 747 648 417 359 330 186 159 48 MspA1I CmG'CkG Cuts at: 1606 2715 2890 3003 3091 3171 3250 3323 3336 Size: 1109 175 113 88 80 79 73 13 Cuts at: 3336 3600 4277 5425 5670 1606 Size: 264 677 1148 245 2120 Fragments arranged by size: 2120 1148 1109 677 264 245 175 113 88 80 79 73 13 NaeI GCC'GGC Cuts at: 2481 2628 2481 Size: 147 6037 NarI GG'CG_CC Cuts at: 2605 2699 3267 2605 Size: 94 568 5522 Fragments arranged by size: 5522 568 94 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5632 NdeI CA'TA_TG Cuts at: 387 387 Size: 6184 NgoMIV G'CCGG_C Cuts at: 2479 2626 2479 Size: 147 6037 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6184 NotI GC'GGCC_GC Cuts at: 3631 3631 Size: 6184 NspI r_CATG'y Cuts at: 1132 1229 3542 5087 1132 Size: 97 2313 1545 2229 Fragments arranged by size: 2313 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2514 1348 Size: 1166 5018 PciI A'CATG_T Cuts at: 5083 5083 Size: 6184 PflMI CCAn_nnn'nTGG Cuts at: 1108 2839 1108 Size: 1731 4453 PfoI T'CCnGG_A Cuts at: 2547 2547 Size: 6184 PmeI GTTT'AAAC Cuts at: 3488 3488 Size: 6184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4253 4285 5766 5798 4253 Size: 32 1481 32 4639 Fragments arranged by size: 4639 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2298 1613 Size: 685 5499 PsiI TTA'TAA Cuts at: 3754 3754 Size: 6184 PspOMI G'GGCC_C Cuts at: 2708 2708 Size: 6184 PstI C_TGCA'G Cuts at: 830 2316 2802 3536 830 Size: 1486 486 734 3478 Fragments arranged by size: 3478 1486 734 486 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6184 PvuII CAG'CTG Cuts at: 2890 3003 3091 3250 2890 Size: 113 88 159 5824 Fragments arranged by size: 5824 159 113 88 SacI G_AGCT'C Cuts at: 2763 3129 2763 Size: 366 5818 SacII CC_GC'GG Cuts at: 3172 3324 3172 Size: 152 6032 SalI G'TCGA_C Cuts at: 1936 2522 3526 1936 Size: 586 1004 4594 Fragments arranged by size: 4594 1004 586 SapI GCTCTTCn'nnn_ Cuts at: 3134 3134 Size: 6184 SbfI CC_TGCA'GG Cuts at: 3536 3536 Size: 6184 ScaI AGT'ACT Cuts at: 2945 4432 6144 2945 Size: 1487 1712 2985 Fragments arranged by size: 2985 1712 1487 SfcI C'TryA_G Cuts at: 826 1045 2312 2437 2560 2798 3532 4667 5348 Size: 219 1267 125 123 238 734 1135 681 Cuts at: 5348 5539 826 Size: 191 1471 Fragments arranged by size: 1471 1267 1135 734 681 238 219 191 125 123 SfoI GGC'GCC Cuts at: 2606 2700 3268 2606 Size: 94 568 5522 Fragments arranged by size: 5522 568 94 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 SgrAI Cr'CCGG_yG Cuts at: 1700 2328 1700 Size: 628 5556 SmaI CCC'GGG Cuts at: 1872 3511 1872 Size: 1639 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2723 4288 5189 5451 820 Size: 197 926 780 1565 901 262 1553 Fragments arranged by size: 1565 1553 926 901 780 262 197 SnaBI TAC'GTA Cuts at: 493 3975 493 Size: 3482 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6184 SphI G_CATG'C Cuts at: 1132 3542 1132 Size: 2410 3774 SspI AAT'ATT Cuts at: 5 52 4108 5 Size: 47 4056 2081 Fragments arranged by size: 4056 2081 47 StuI AGG'CCT Cuts at: 1517 3443 1517 Size: 1926 4258 StyI C'CwwG_G Cuts at: 513 1065 2742 3623 513 Size: 552 1677 881 3074 Fragments arranged by size: 3074 1677 881 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2885 4211 4228 4381 4566 5011 325 Size: 1255 1305 1326 17 153 185 445 1498 Fragments arranged by size: 1498 1326 1305 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2943 4430 6142 Size: 274 80 33 51 1443 966 1487 1712 Cuts at: 6142 96 Size: 138 Fragments arranged by size: 1712 1487 1443 966 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6144 6176 6144 Size: 32 6152 Tth111I GACn'n_nGTC Cuts at: 1316 2380 1316 Size: 1064 5120 XbaI T'CTAG_A Cuts at: 3520 3520 Size: 6184 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6184 XmaI C'CCGG_G Cuts at: 1870 3509 1870 Size: 1639 4545 XmnI GAAnn'nnTTC Cuts at: 4313 4313 Size: 6184 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3241 3992 276 Size: 53 83 186 936 1707 751 2468 Fragments arranged by size: 2468 1707 936 751 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI XcmI