(Linear) (Six-base) MAPSORT of: KIEE4474.seq Check: 590 from: 1 to: 1479 KIEE4474.orf With 182 enzymes: SgfI * February 29, 2012 11:54 .. AatII G_ACGT'C Cuts at: 0 1238 1479 Size: 1238 241 AccI GT'mk_AC Cuts at: 0 518 1479 Size: 518 961 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 1429 1453 1479 Size: 139 1290 24 26 Fragments arranged by size: 1290 139 26 24 AfeI AGC'GCT Cuts at: 0 451 553 1479 Size: 451 102 926 Fragments arranged by size: 926 451 102 AflIII A'CryG_T Cuts at: 0 101 815 1479 Size: 101 714 664 Fragments arranged by size: 714 664 101 AhdI GACnn_n'nnGTC Cuts at: 0 354 1479 Size: 354 1125 AleI CACnn'nnGTG Cuts at: 0 739 1342 1479 Size: 739 603 137 Fragments arranged by size: 739 603 137 ApaI G_GGCC'C Cuts at: 0 707 1479 Size: 707 772 ApaLI G'TGCA_C Cuts at: 0 400 1479 Size: 400 1079 AscI GG'CGCG_CC Cuts at: 0 610 1479 Size: 610 869 AvaI C'yCGr_G Cuts at: 0 616 1479 Size: 616 863 BanI G'GyrC_C Cuts at: 0 249 340 599 693 1204 1261 1479 Size: 249 91 259 94 511 57 218 Fragments arranged by size: 511 259 249 218 94 91 57 BanII G_rGCy'C Cuts at: 0 707 758 1124 1479 Size: 707 51 366 355 Fragments arranged by size: 707 366 355 51 BbeI G_GCGC'C Cuts at: 0 603 697 1265 1479 Size: 603 94 568 214 Fragments arranged by size: 603 568 214 94 BbsI GAAGACnn'nnnn_ Cuts at: 0 1230 1479 Size: 1230 249 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 742 776 1479 Size: 742 34 703 Fragments arranged by size: 742 703 34 BglI GCCn_nnn'nGGC Cuts at: 0 197 702 1479 Size: 197 505 777 Fragments arranged by size: 777 505 197 BglII A'GATC_T Cuts at: 0 131 1479 Size: 131 1348 Bme1580I G_kGCm'C Cuts at: 0 254 404 509 707 1207 1479 Size: 254 150 105 198 500 272 Fragments arranged by size: 500 272 254 198 150 105 BmgBI CAC'GTC Cuts at: 0 504 1479 Size: 504 975 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 666 1479 Size: 666 813 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 661 787 907 966 1209 1267 1479 Size: 163 498 126 120 59 243 58 212 Fragments arranged by size: 498 243 212 163 126 120 59 58 Bpu10I CC'TnA_GC Cuts at: 0 463 1479 Size: 463 1016 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 703 1479 Size: 703 776 BsaI GGTCTCn'nnnn_ Cuts at: 0 856 868 1479 Size: 856 12 611 Fragments arranged by size: 856 611 12 BsaHI Gr'CG_yC Cuts at: 0 515 600 694 1045 1214 1235 1262 1427 Size: 515 85 94 351 169 21 27 165 Cuts at: 1427 1479 Size: 52 Fragments arranged by size: 515 351 169 165 94 85 52 27 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 874 1479 Size: 874 605 BseYI C'CCAG_C Cuts at: 0 706 1479 Size: 706 773 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 583 988 1479 Size: 49 534 405 491 Fragments arranged by size: 534 491 405 49 BsiEI CG_ry'CG Cuts at: 0 77 176 297 475 479 897 1479 Size: 77 99 121 178 4 418 582 Fragments arranged by size: 582 418 178 121 99 77 4 BsiHKAI G_wGCw'C Cuts at: 0 404 758 1124 1479 Size: 404 354 366 355 Fragments arranged by size: 404 366 355 354 BsmI GAATG_Cn' Cuts at: 0 996 1479 Size: 996 483 Bsp1286I G_dGCh'C Cuts at: 0 254 404 509 707 758 1124 1207 1479 Size: 254 150 105 198 51 366 83 272 Fragments arranged by size: 366 272 254 198 150 105 83 51 BspHI T'CATG_A Cuts at: 0 1130 1479 Size: 1130 349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 244 1479 Size: 244 1235 BsrBI CCG'CTC Cuts at: 0 650 1223 1479 Size: 650 573 256 Fragments arranged by size: 650 573 256 BsrFI r'CCGG_y Cuts at: 0 323 474 621 1025 1479 Size: 323 151 147 404 454 Fragments arranged by size: 454 404 323 151 147 BssHII G'CGCG_C Cuts at: 0 467 610 1479 Size: 467 143 869 Fragments arranged by size: 869 467 143 BstEII G'GTnAC_C Cuts at: 0 1320 1479 Size: 1320 159 BstXI CCAn_nnnn'nTGG Cuts at: 0 741 1392 1479 Size: 741 651 87 Fragments arranged by size: 741 651 87 BstYI r'GATC_y Cuts at: 0 32 131 1479 Size: 32 99 1348 Fragments arranged by size: 1348 99 32 Bsu36I CC'TnA_GG Cuts at: 0 419 1479 Size: 419 1060 BtgI C'CryG_G Cuts at: 0 491 1164 1316 1451 1479 Size: 491 673 152 135 28 Fragments arranged by size: 673 491 152 135 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 390 1479 Size: 390 1089 BtsI GCAGTG_nn' Cuts at: 0 1314 1479 Size: 1314 165 DrdI GACnn_nn'nnGTC Cuts at: 0 1318 1479 Size: 1318 161 EaeI y'GGCC_r Cuts at: 0 74 173 472 476 623 800 953 1088 Size: 74 99 299 4 147 177 153 135 Cuts at: 1088 1382 1479 Size: 294 97 Fragments arranged by size: 299 294 177 153 147 135 99 97 74 4 EagI C'GGCC_G Cuts at: 0 74 173 472 476 1479 Size: 74 99 299 4 1003 Fragments arranged by size: 1003 299 99 74 4 EarI CTCTTCn'nnn_ Cuts at: 0 1129 1479 Size: 1129 350 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 96 229 1459 1479 Size: 96 133 1230 20 Fragments arranged by size: 1230 133 96 20 EcoICRI GAG'CTC Cuts at: 0 756 1122 1479 Size: 756 366 357 Fragments arranged by size: 756 366 357 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 163 661 787 907 966 1209 1267 Size: 139 24 498 126 120 59 243 58 Cuts at: 1267 1429 1453 1479 Size: 162 24 26 Fragments arranged by size: 498 243 162 139 126 120 59 58 26 24 24 EcoO109I rG'GnC_Cy Cuts at: 0 703 1479 Size: 703 776 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 681 933 1479 Size: 681 252 546 Fragments arranged by size: 681 546 252 FseI GG_CCGG'CC Cuts at: 0 478 1479 Size: 478 1001 FspI TGC'GCA Cuts at: 0 187 1479 Size: 187 1292 HaeII r_GCGC'y Cuts at: 0 330 453 555 603 697 1265 1404 1479 Size: 330 123 102 48 94 568 139 75 Fragments arranged by size: 568 330 139 123 102 94 75 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 340 372 1447 1479 Size: 340 32 1075 32 Fragments arranged by size: 1075 340 32 32 HincII GTy'rAC Cuts at: 0 426 519 1479 Size: 426 93 960 Fragments arranged by size: 960 426 93 KasI G'GCGC_C Cuts at: 0 599 693 1261 1479 Size: 599 94 568 218 Fragments arranged by size: 599 568 218 94 MluI A'CGCG_T Cuts at: 0 101 1479 Size: 101 1378 MscI TGG'CCA Cuts at: 0 1384 1479 Size: 1384 95 MslI CAynn'nnrTG Cuts at: 0 409 739 1156 1342 1390 1479 Size: 409 330 417 186 48 89 Fragments arranged by size: 417 409 330 186 89 48 MspA1I CmG'CkG Cuts at: 0 710 885 998 1086 1166 1245 1318 1331 Size: 710 175 113 88 80 79 73 13 Cuts at: 1331 1479 Size: 148 Fragments arranged by size: 710 175 148 113 88 80 79 73 13 NaeI GCC'GGC Cuts at: 0 476 623 1479 Size: 476 147 856 Fragments arranged by size: 856 476 147 NarI GG'CG_CC Cuts at: 0 600 694 1262 1479 Size: 600 94 568 217 Fragments arranged by size: 600 568 217 94 NgoMIV G'CCGG_C Cuts at: 0 474 621 1479 Size: 474 147 858 Fragments arranged by size: 858 474 147 PasI CC'CwG_GG Cuts at: 0 509 1479 Size: 509 970 PflMI CCAn_nnn'nTGG Cuts at: 0 834 1479 Size: 834 645 PfoI T'CCnGG_A Cuts at: 0 542 1479 Size: 542 937 PshAI GACnn'nnGTC Cuts at: 0 293 1479 Size: 293 1186 PspOMI G'GGCC_C Cuts at: 0 703 1479 Size: 703 776 PstI C_TGCA'G Cuts at: 0 311 797 1479 Size: 311 486 682 Fragments arranged by size: 682 486 311 PvuII CAG'CTG Cuts at: 0 885 998 1086 1245 1479 Size: 885 113 88 159 234 Fragments arranged by size: 885 234 159 113 88 SacI G_AGCT'C Cuts at: 0 758 1124 1479 Size: 758 366 355 Fragments arranged by size: 758 366 355 SacII CC_GC'GG Cuts at: 0 1167 1319 1479 Size: 1167 152 160 Fragments arranged by size: 1167 160 152 SalI G'TCGA_C Cuts at: 0 517 1479 Size: 517 962 SapI GCTCTTCn'nnn_ Cuts at: 0 1129 1479 Size: 1129 350 ScaI AGT'ACT Cuts at: 0 940 1479 Size: 940 539 SfcI C'TryA_G Cuts at: 0 307 432 555 793 1479 Size: 307 125 123 238 686 Fragments arranged by size: 686 307 238 125 123 SfoI GGC'GCC Cuts at: 0 601 695 1263 1479 Size: 601 94 568 216 Fragments arranged by size: 601 568 216 94 SgrAI Cr'CCGG_yG Cuts at: 0 323 1479 Size: 323 1156 SmlI C'TyrA_G Cuts at: 0 718 1479 Size: 718 761 StuI AGG'CCT Cuts at: 0 1438 1479 Size: 1438 41 StyI C'CwwG_G Cuts at: 0 737 1479 Size: 737 742 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 880 1479 Size: 880 599 TatI w'GTAC_w Cuts at: 0 938 1479 Size: 938 541 Tth111I GACn'n_nGTC Cuts at: 0 375 1479 Size: 375 1104 ZraI GAC'GTC Cuts at: 0 1236 1479 Size: 1236 243 Enzymes that do cut: AatII AccI AcuI AfeI AflIII AhdI AleI ApaI ApaLI AscI AvaI BanI BanII BbeI BbsI BcgI BglI BglII Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI FseI FspI HaeII Hin4I HincII KasI MluI MscI MslI MspA1I NaeI NarI NgoMIV PasI PflMI PfoI PshAI PspOMI PstI PvuII SacI SacII SalI SapI ScaI SfcI SfoI SgrAI SmlI StuI StyI TaqII TatI Tth111I ZraI Enzymes that do not cut: AarI Acc65I AclI AflII AgeI AlfI AloI AlwNI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BlpI BmrI BmtI BsaAI BsaBI BsaWI BsaXI BsiWI BsmBI BspEI BsrDI BsrGI BssSI BstAPI BstBI BstZ17I ClaI CspCI DraI DraIII EcoNI EcoRI EcoRV FspAI HindIII HpaI KpnI MfeI MmeI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SanDI SbfI SexAI SfiI SgfI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI XcmI XhoI XmaI XmnI