Restriction map for
pFN21AB5481
(Linear) (Six-base) MAPSORT of: KIBB5481.seq Check: 3992 from: 1 to: 1359
KIBB5481.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 445 850 1222 1359
Size: 204 241 405 372 137
Fragments arranged by size:
405 372 241 204 137
AflIII A'CryG_T
Cuts at: 0 891 1359
Size: 891 468
AlwNI CAG_nnn'CTG
Cuts at: 0 997 1039 1359
Size: 997 42 320
Fragments arranged by size:
997 320 42
ApoI r'AATT_y
Cuts at: 0 433 525 1326 1359
Size: 433 92 801 33
Fragments arranged by size:
801 433 92 33
AvaI C'yCGr_G
Cuts at: 0 30 1359
Size: 30 1329
BbsI GAAGACnn'nnnn_
Cuts at: 0 1154 1359
Size: 1154 205
BbvCI CC'TCA_GC
Cuts at: 0 467 1359
Size: 467 892
BciVI GTATCCnnnnn_n'
Cuts at: 0 1135 1359
Size: 1135 224
BfrBI ATG'CAT
Cuts at: 0 853 1359
Size: 853 506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1061 1359
Size: 1061 298
Bpu10I CC'TnA_GC
Cuts at: 0 467 1359
Size: 467 892
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 1070 1359
Size: 133 937 289
Fragments arranged by size:
937 289 133
BsaWI w'CCGG_w
Cuts at: 0 545 1359
Size: 545 814
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 622 1359
Size: 622 737
BseYI C'CCAG_C
Cuts at: 0 883 1359
Size: 883 476
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 860 910 1359
Size: 860 50 449
Fragments arranged by size:
860 449 50
BsiHKAI G_wGCw'C
Cuts at: 0 93 1359
Size: 93 1266
BsmI GAATG_Cn'
Cuts at: 0 376 555 1359
Size: 376 179 804
Fragments arranged by size:
804 376 179
Bsp1286I G_dGCh'C
Cuts at: 0 93 1359
Size: 93 1266
BsrFI r'CCGG_y
Cuts at: 0 19 1359
Size: 19 1340
BstAPI GCAn_nnn'nTGC
Cuts at: 0 823 979 1359
Size: 823 156 380
Fragments arranged by size:
823 380 156
Bsu36I CC'TnA_GG
Cuts at: 0 104 1359
Size: 104 1255
BtgI C'CryG_G
Cuts at: 0 300 759 1359
Size: 300 459 600
Fragments arranged by size:
600 459 300
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 741 776 1359
Size: 741 35 583
Fragments arranged by size:
741 583 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 796 1359
Size: 796 563
EaeI y'GGCC_r
Cuts at: 0 1299 1359
Size: 1299 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 445 850 1061 1222 1359
Size: 204 241 405 211 161 137
Fragments arranged by size:
405 241 211 204 161 137
EcoO109I rG'GnC_Cy
Cuts at: 0 108 1190 1359
Size: 108 1082 169
Fragments arranged by size:
1082 169 108
EcoRV GAT'ATC
Cuts at: 0 1269 1359
Size: 1269 90
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 671 1111 1359
Size: 671 440 248
Fragments arranged by size:
671 440 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 755 787 1034 1066 1359
Size: 755 32 247 32 293
Fragments arranged by size:
755 293 247 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1124 1359
Size: 1124 235
MscI TGG'CCA
Cuts at: 0 1301 1359
Size: 1301 58
MspA1I CmG'CkG
Cuts at: 0 76 1036 1359
Size: 76 960 323
Fragments arranged by size:
960 323 76
NaeI GCC'GGC
Cuts at: 0 21 1359
Size: 21 1338
NgoMIV G'CCGG_C
Cuts at: 0 19 1359
Size: 19 1340
NsiI A_TGCA'T
Cuts at: 0 855 1359
Size: 855 504
NspI r_CATG'y
Cuts at: 0 535 874 895 1221 1359
Size: 535 339 21 326 138
Fragments arranged by size:
535 339 326 138 21
PciI A'CATG_T
Cuts at: 0 891 1359
Size: 891 468
PfoI T'CCnGG_A
Cuts at: 0 1175 1359
Size: 1175 184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 695 727 1359
Size: 695 32 632
Fragments arranged by size:
695 632 32
PpuMI rG'GwC_Cy
Cuts at: 0 108 1190 1359
Size: 108 1082 169
Fragments arranged by size:
1082 169 108
PshAI GACnn'nnGTC
Cuts at: 0 514 1359
Size: 514 845
PstI C_TGCA'G
Cuts at: 0 87 1359
Size: 87 1272
PvuII CAG'CTG
Cuts at: 0 76 1036 1359
Size: 76 960 323
Fragments arranged by size:
960 323 76
SanDI GG'GwC_CC
Cuts at: 0 1190 1359
Size: 1190 169
SbfI CC_TGCA'GG
Cuts at: 0 87 1359
Size: 87 1272
SfcI C'TryA_G
Cuts at: 0 83 582 1359
Size: 83 499 777
Fragments arranged by size:
777 499 83
SmlI C'TyrA_G
Cuts at: 0 30 112 1085 1359
Size: 30 82 973 274
Fragments arranged by size:
973 274 82 30
SphI G_CATG'C
Cuts at: 0 535 1359
Size: 535 824
StyI C'CwwG_G
Cuts at: 0 554 941 1359
Size: 554 387 418
Fragments arranged by size:
554 418 387
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 253 1359
Size: 253 1106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 656 688 1359
Size: 656 32 671
Fragments arranged by size:
671 656 32
XhoI C'TCGA_G
Cuts at: 0 30 1359
Size: 30 1329
XmnI GAAnn'nnTTC
Cuts at: 0 634 707 1095 1359
Size: 634 73 388 264
Fragments arranged by size:
634 388 264 73
Enzymes that do cut:
AcuI AflIII AlwNI ApoI AvaI BbsI BbvCI BciVI
BfrBI BpmI Bpu10I BpuEI BsaWI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BsrFI BstAPI Bsu36I BtgI CspCI
DrdI EaeI Eco57MI EcoO109I EcoRV FalI Hin4I MmeI
MscI MspA1I NaeI NgoMIV NsiI NspI PciI PfoI
PpiI PpuMI PshAI PstI PvuII SanDI SbfI SfcI
SmlI SphI StyI TaqII TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BanII BbeI BcgI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BsaI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I BtgZI BtsI
ClaI DraI DraIII EagI EarI EciI EcoICRI EcoNI
EcoRI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MslI NarI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PmeI PmlI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TatI
Tth111I XbaI XcmI XmaI ZraI