(Circular) (Six-base) MAPSORT of: pFN21AB9947.seq Check: 43 from: 1 to: 6298 pFN21AB9947 6298 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4108 278 Size: 53 83 186 936 2572 2468 Fragments arranged by size: 2572 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3641 1937 Size: 1704 4594 Acc65I G'GTAC_C Cuts at: 3619 3619 Size: 6298 AclI AA'CG_TT Cuts at: 1563 4425 4798 1563 Size: 2862 373 3063 Fragments arranged by size: 3063 2862 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2426 2711 3054 4361 5745 6013 1632 Size: 794 285 343 1307 1384 268 1917 Fragments arranged by size: 1917 1384 1307 794 343 285 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6101 AflIII A'CryG_T Cuts at: 1203 2091 5197 6174 1203 Size: 888 3106 977 1327 Fragments arranged by size: 3106 1327 977 888 AgeI A'CCGG_T Cuts at: 5129 5129 Size: 6298 AhdI GACnn_n'nnGTC Cuts at: 3384 5027 3384 Size: 1643 4655 AleI CACnn'nnGTG Cuts at: 1166 3242 1166 Size: 2076 4222 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2586 3162 1184 Size: 1402 576 4320 Fragments arranged by size: 4320 1402 576 AlwNI CAG_nnn'CTG Cuts at: 2056 2689 2995 3142 3164 4041 5613 2056 Size: 633 306 147 22 877 1572 2741 Fragments arranged by size: 2741 1572 877 633 306 147 22 ApaLI G'TGCA_C Cuts at: 4355 5511 4355 Size: 1156 5142 ApoI r'AATT_y Cuts at: 1493 3607 3833 3985 6094 1493 Size: 2114 226 152 2109 1697 Fragments arranged by size: 2114 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4852 160 Size: 872 3820 1606 Fragments arranged by size: 3820 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 AvaI C'yCGr_G Cuts at: 1870 1943 3027 3501 3623 1870 Size: 73 1084 474 122 4545 Fragments arranged by size: 4545 1084 474 122 73 BamHI G'GATC_C Cuts at: 3628 3995 3628 Size: 367 5931 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2570 3208 3248 3549 3619 Size: 325 217 627 783 638 40 301 70 Cuts at: 3619 5074 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 783 638 627 325 301 217 70 40 BanII G_rGCy'C Cuts at: 1391 2373 3617 1391 Size: 982 1244 4072 Fragments arranged by size: 4072 1244 982 BbeI G_GCGC'C Cuts at: 3212 3553 3212 Size: 341 5957 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2914 928 Size: 971 1015 4312 Fragments arranged by size: 4312 1015 971 BbvCI CC'TCA_GC Cuts at: 3453 3453 Size: 6298 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4489 4523 1903 Size: 34 2552 34 3678 Fragments arranged by size: 3678 2552 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4190 5400 4190 Size: 1210 5088 BclI T'GATC_A Cuts at: 1548 3006 1548 Size: 1458 4840 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3155 3257 4909 Size: 107 122 71 631 605 1483 102 1652 Cuts at: 4909 136 Size: 1525 Fragments arranged by size: 1652 1525 1483 631 605 122 107 102 71 BglII A'GATC_T Cuts at: 2523 6293 2523 Size: 3770 2528 BlpI GC'TnA_GC Cuts at: 3715 3715 Size: 6298 Bme1580I G_kGCm'C Cuts at: 1790 3349 4359 5515 1790 Size: 1559 1010 1156 2573 Fragments arranged by size: 2573 1559 1156 1010 BmgBI CAC'GTC Cuts at: 1318 3460 1318 Size: 2142 4156 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2376 3428 4029 Size: 416 53 83 399 24 959 1052 601 Cuts at: 4029 4987 442 Size: 958 1753 Fragments arranged by size: 1753 1052 959 958 601 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6298 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2438 2438 Size: 6298 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 2537 2876 3017 3111 3140 4958 1239 Size: 866 432 339 141 94 29 1818 2579 Fragments arranged by size: 2579 1818 866 432 339 141 94 29 Bpu10I CC'TnA_GC Cuts at: 3453 3453 Size: 6298 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4423 5288 5586 4423 Size: 865 298 5135 Fragments arranged by size: 5135 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2255 4961 882 Size: 617 756 2706 2219 Fragments arranged by size: 2706 2219 756 617 BsaAI yAC'GTr Cuts at: 493 1204 2530 3344 4089 493 Size: 711 1326 814 745 2702 Fragments arranged by size: 2702 1326 814 745 711 BsaBI GATnn'nnATC Cuts at: 6292 6292 Size: 6298 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3209 3550 4105 4487 Size: 53 83 186 936 1676 341 555 382 Cuts at: 4487 5122 275 Size: 635 1451 Fragments arranged by size: 1676 1451 936 635 555 382 341 186 83 53 BsaWI w'CCGG_w Cuts at: 3542 4731 5129 5403 5550 5961 3542 Size: 1189 398 274 147 411 3879 Fragments arranged by size: 3879 1189 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2078 2108 3513 3543 1178 Size: 30 165 30 675 30 1405 30 3933 Fragments arranged by size: 3933 1405 675 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6298 BseYI C'CCAG_C Cuts at: 2049 2161 2963 5501 5997 2049 Size: 112 802 2538 496 2350 Fragments arranged by size: 2538 2350 802 496 112 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2042 1163 Size: 573 306 5419 Fragments arranged by size: 5419 573 306 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3748 4509 5537 1540 Size: 275 186 1747 761 1028 2301 Fragments arranged by size: 2301 1747 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2091 4359 4444 5515 2091 Size: 2268 85 1071 2874 Fragments arranged by size: 2874 2268 1071 85 BsmI GAATG_Cn' Cuts at: 3809 3902 3809 Size: 93 6205 BsmBI CGTCTCn'nnnn_ Cuts at: 2174 2174 Size: 6298 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2373 3349 3617 4359 4444 5515 Size: 399 301 282 976 268 742 85 1071 Cuts at: 5515 1391 Size: 2174 Fragments arranged by size: 2174 1071 976 742 399 301 282 268 85 BspEI T'CCGG_A Cuts at: 5961 5961 Size: 6298 BspHI T'CATG_A Cuts at: 2245 2824 4187 5917 2245 Size: 579 1363 1730 2626 Fragments arranged by size: 2626 1730 1363 579 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3300 3653 844 Size: 2456 353 3489 Fragments arranged by size: 3489 2456 353 BsrBI CCG'CTC Cuts at: 1394 1823 4185 1394 Size: 429 2362 3507 Fragments arranged by size: 3507 2362 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3354 4793 4967 65 Size: 1183 2106 1439 174 1396 Fragments arranged by size: 2106 1439 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2319 3595 4942 5129 1700 Size: 110 509 1276 1347 187 2869 Fragments arranged by size: 2869 1347 1276 509 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6298 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6298 BssSI C'ACGA_G Cuts at: 2883 4358 5370 2883 Size: 1475 1012 3811 Fragments arranged by size: 3811 1475 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3164 794 Size: 2370 3928 BstBI TT'CG_AA Cuts at: 5102 5102 Size: 6298 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6298 BstXI CCAn_nnnn'nTGG Cuts at: 3244 3244 Size: 6298 BstYI r'GATC_y Cuts at: 1972 2358 2523 2686 3628 3995 4380 4397 5849 Size: 386 165 163 942 367 385 17 1452 Cuts at: 5849 5935 5947 6293 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 942 386 385 367 346 165 163 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2067 2286 3243 3252 3403 513 Size: 552 1002 219 957 9 151 3408 Fragments arranged by size: 3408 1002 957 552 219 151 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5559 Fragments arranged by size: 5559 683 56 BtsI GCAGTG_nn' Cuts at: 2388 3046 3283 3814 4608 4628 2388 Size: 658 237 531 794 20 4058 Fragments arranged by size: 4058 794 658 531 237 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6298 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6263 DraI TTT'AAA Cuts at: 3602 3602 Size: 6298 DraIII CAC_nnn'GTG Cuts at: 3325 3325 Size: 6298 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5305 809 Size: 507 3989 1802 Fragments arranged by size: 3989 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3048 3327 3745 Size: 54 1425 50 275 18 1218 279 418 Cuts at: 3745 4634 6263 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1218 889 418 279 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3745 1537 Size: 275 1933 4090 Fragments arranged by size: 4090 1933 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4228 1353 Size: 2875 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3188 4885 5259 5405 387 Size: 1064 138 1599 1697 374 146 1280 Fragments arranged by size: 1697 1599 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2426 2537 2711 2876 3017 3054 Size: 393 473 321 111 174 165 141 37 Cuts at: 3054 3111 3140 4361 4958 5745 6013 1239 Size: 57 29 1221 597 787 268 1524 Fragments arranged by size: 1524 1221 787 597 473 393 321 268 174 165 141 111 57 37 29 EcoO109I rG'GnC_Cy Cuts at: 2351 2773 3430 2351 Size: 422 657 5219 Fragments arranged by size: 5219 657 422 EcoRI G'AATT_C Cuts at: 3607 3985 3607 Size: 378 5920 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2316 2316 Size: 6298 FspI TGC'GCA Cuts at: 2279 3131 4804 2279 Size: 852 1673 3773 Fragments arranged by size: 3773 1673 852 FspAI rTGC'GCAy Cuts at: 2279 3131 2279 Size: 852 5446 HaeII r_GCGC'y Cuts at: 1678 3212 3553 5445 1678 Size: 1534 341 1892 2531 Fragments arranged by size: 2531 1892 1534 341 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2343 2375 3355 3370 3387 Size: 32 135 32 771 32 980 15 17 Cuts at: 3387 3402 3513 3545 4939 4971 5013 5045 1373 Size: 15 111 32 1394 32 42 32 2626 Fragments arranged by size: 2626 1394 980 771 135 111 42 32 32 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 3642 3888 669 Size: 988 281 1704 246 3079 Fragments arranged by size: 3079 1704 988 281 246 HindIII A'AGCT_T Cuts at: 748 2293 748 Size: 1545 4753 HpaI GTT'AAC Cuts at: 3888 3888 Size: 6298 KasI G'GCGC_C Cuts at: 3208 3549 3208 Size: 341 5957 KpnI G_GTAC'C Cuts at: 3623 3623 Size: 6298 MfeI C'AATT_G Cuts at: 3897 3897 Size: 6298 MluI A'CGCG_T Cuts at: 6174 6174 Size: 6298 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2649 5412 5596 1305 Size: 1344 2763 184 2007 Fragments arranged by size: 2763 2007 1344 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3050 3329 10 Size: 54 1425 343 1218 279 2979 Fragments arranged by size: 2979 1425 1218 343 279 54 MslI CAynn'nnrTG Cuts at: 518 1166 3242 3359 4256 4615 4774 518 Size: 648 2076 117 897 359 159 2042 Fragments arranged by size: 2076 2042 897 648 359 159 117 MspA1I CmG'CkG Cuts at: 1606 2056 2385 3714 4391 5539 5784 1606 Size: 450 329 1329 677 1148 245 2120 Fragments arranged by size: 2120 1329 1148 677 450 329 245 NarI GG'CG_CC Cuts at: 3209 3550 3209 Size: 341 5957 NcoI C'CATG_G Cuts at: 513 1065 2067 513 Size: 552 1002 4744 Fragments arranged by size: 4744 1002 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6298 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6298 NotI GC'GGCC_GC Cuts at: 3745 3745 Size: 6298 NspI r_CATG'y Cuts at: 1132 1229 2095 3136 3656 5201 1132 Size: 97 866 1041 520 1545 2229 Fragments arranged by size: 2229 1545 1041 866 520 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6298 PciI A'CATG_T Cuts at: 2091 5197 2091 Size: 3106 3192 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6298 PfoI T'CCnGG_A Cuts at: 2082 2682 2853 3060 3438 2082 Size: 600 171 207 378 4942 Fragments arranged by size: 4942 600 378 207 171 PmeI GTTT'AAAC Cuts at: 3602 3602 Size: 6298 PmlI CAC'GTG Cuts at: 3344 3344 Size: 6298 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4367 4399 5880 5912 4367 Size: 32 1481 32 4753 Fragments arranged by size: 4753 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2351 2773 3430 2351 Size: 422 657 5219 Fragments arranged by size: 5219 657 422 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6298 PsiI TTA'TAA Cuts at: 3868 3868 Size: 6298 PstI C_TGCA'G Cuts at: 830 2395 2914 3278 3650 830 Size: 1565 519 364 372 3478 Fragments arranged by size: 3478 1565 519 372 364 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6298 PvuII CAG'CTG Cuts at: 2056 2056 Size: 6298 SalI G'TCGA_C Cuts at: 1936 3640 1936 Size: 1704 4594 SbfI CC_TGCA'GG Cuts at: 3650 3650 Size: 6298 ScaI AGT'ACT Cuts at: 4546 6258 4546 Size: 1712 4586 SexAI A'CCwGG_T Cuts at: 3321 3321 Size: 6298 SfcI C'TryA_G Cuts at: 826 1045 2391 2910 3274 3646 4781 5462 5653 Size: 219 1346 519 364 372 1135 681 191 Cuts at: 5653 826 Size: 1471 Fragments arranged by size: 1471 1346 1135 681 519 372 364 219 191 SfoI GGC'GCC Cuts at: 3210 3551 3210 Size: 341 5957 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6298 SmaI CCC'GGG Cuts at: 1872 3625 1872 Size: 1753 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4402 5303 5565 820 Size: 197 926 2459 901 262 1553 Fragments arranged by size: 2459 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4089 493 Size: 3596 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6298 SphI G_CATG'C Cuts at: 1132 3136 3656 1132 Size: 2004 520 3774 Fragments arranged by size: 3774 2004 520 SspI AAT'ATT Cuts at: 5 52 4222 5 Size: 47 4170 2081 Fragments arranged by size: 4170 2081 47 StuI AGG'CCT Cuts at: 1517 3260 3446 1517 Size: 1743 186 4369 Fragments arranged by size: 4369 1743 186 StyI C'CwwG_G Cuts at: 513 1065 2067 2610 3737 513 Size: 552 1002 543 1127 3074 Fragments arranged by size: 3074 1127 1002 552 543 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2134 3236 4325 4342 4495 4680 5125 Size: 1255 554 1102 1089 17 153 185 445 Cuts at: 5125 325 Size: 1498 Fragments arranged by size: 1498 1255 1102 1089 554 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4544 6256 96 Size: 274 80 33 51 1443 2567 1712 138 Fragments arranged by size: 2567 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2078 2110 2199 2231 6258 6290 2078 Size: 32 89 32 4027 32 2086 Fragments arranged by size: 4027 2086 89 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3434 1316 Size: 2118 4180 XbaI T'CTAG_A Cuts at: 2338 3634 2338 Size: 1296 5002 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3058 3401 3058 Size: 343 5955 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6298 XmaI C'CCGG_G Cuts at: 1870 3623 1870 Size: 1753 4545 XmnI GAAnn'nnTTC Cuts at: 2921 4427 2921 Size: 1506 4792 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4106 276 Size: 53 83 186 936 2572 2468 Fragments arranged by size: 2572 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI