################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BcgI BcnI BfrI BglI BglII BlnI BpuAI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I FauI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MslI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AclI 1030 AACGTT AA^CGTT AlwNI 122 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 621 GGACC G^G[AT]CC BanI 1110 GGCACC G^G[CT][GA]CC BanII 770 GGGCTC G[GA]GC[CT]^C BbvCI 48 GCTGAGG CCTCAGC(-5/-2) BciVI 1212 GGATAC GTATCC(6/5) BmyI 770 GGGCTC G[AGT]GC[ACT]^C BpmI 773 CTCCAG CTGGAG(16/14) BsiEI 39 CGACCG CG[GA][CT]^CG BsmI 1483 GCATTC GAATGC(1/-1) Bsp1286I 770 GGGCTC G[AGT]GC[ACT]^C BsrDI 1476 CATTGC GCAATG(2/0) BsrFI 41 ACCGGC [GA]^CCGG[CT] BssSI 923 CTCGTG CACGAG(-5/-1) BstXI 904 CCATCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 4 GCGC GCG^C Cfr10I 41 ACCGGC [GA]^CCGG[CT] Cfr13I 621 GGACC G^G[ACGT]CC EcoNI 970 CCTGTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1322 GATATC GAT^ATC EcoT22I 533 ATGCAT ATGCA^T HapII 42 CCGG C^CGG HhaI 4 GCGC GCG^C HinP1I 4 GCGC G^CGC HpaII 42 CCGG C^CGG MspI 42 CCGG C^CGG NsiI 533 ATGCAT ATGCA^T NspI 1571 GCATGT [GA]CATG^[CT] PflMI 154 CCAGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 622 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1030 AACGTT AA^CGTT Sau96I 621 GGACC G^G[ACGT]CC SexAI 153 ACCAGGT A^CC[AT]GGT SpeI 471 ACTAGT A^CTAGT SspI 659 AATATT AAT^ATT Tsp45I 1575 GTCAC ^GT[GC]AC Van91I 154 CCAGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 336 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AciI 1064 CCGC CCGC(-3/-1) 197 GCGG Asp700I 328 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 580 GAACATTTTC BalI 944 TGGCCA TGG^CCA 1104 TGGCCA BclI 63 TGATCA T^GATCA 647 TGATCA BlpI 413 GCTTAGC GC^T[ACGT]AGC 1462 GCTGAGC Bpu1102I 413 GCTTAGC GC^T[ACGT]AGC 1462 GCTGAGC BsaJI 266 CCATGG C^C[ACGT][ACGT]GG 637 CCATGG BsiYI 154 CCAGGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 970 CCTGTTCGAGG BslI 154 CCAGGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 970 CCTGTTCGAGG BsmAI 298 GTCTC GTCTC(1/5) 1379 GTCTC BstYI 543 GGATCT [GA]^GATC[CT] 839 AGATCC CelII 413 GCTTAGC GC^T[ACGT]AGC 1462 GCTGAGC DsaI 266 CCATGG C^C[GA][CT]GG 637 CCATGG EaeI 944 TGGCCA [CT]^GGCC[GA] 1104 TGGCCA EarI 316 GAAGAG CTCTTC(1/4) 784 GAAGAG EcoT14I 266 CCATGG C^C[AT][AT]GG 637 CCATGG FbaI 63 TGATCA T^GATCA 647 TGATCA HaeIII 945 GGCC GG^CC 1105 GGCC Ksp632I 316 GAAGAG CTCTTC(1/4) 784 GAAGAG MaeII 706 ACGT A^CGT 1031 ACGT MaeIII 1284 GTTAC ^GT[ACGT]AC 1575 GTCAC MflI 543 GGATCT [GA]^GATC[CT] 839 AGATCC MluNI 944 TGGCCA TGG^CCA 1104 TGGCCA MscI 944 TGGCCA TGG^CCA 1104 TGGCCA NcoI 266 CCATGG C^CATGG 637 CCATGG NdeI 464 CATATG CA^TATG 607 CATATG NlaIV 771 GGCTCC GG[ACGT]^[ACGT]CC 1110 GGCACC PstI 1093 CTGCAG CTGCA^G 1149 CTGCAG PvuII 7 CAGCTG CAG^CTG 46 CAGCTG SfaNI 205 GATGC GCATC(5/9) 1057 GATGC SmlI 22 CTCAAG C^T[CT][GA]AG 1505 CTCAAG StyI 266 CCATGG C^C[AT][AT]GG 637 CCATGG XhoII 543 GGATCT [GA]^GATC[CT] 839 AGATCC XmnI 328 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 580 GAACATTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbvI BfaI BsaBI BsrI BssKI BstF5I BstNI DdeI DpnII EcoRI EcoRII Fnu4HI FokI HinfI HphI ItaI MaeI MamI MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII PleI RsaI Sau3AI ScrFI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I