(Circular) (Six-base) MAPSORT of: pFN21AE3644.seq Check: 4776 from: 1 to: 6307 pFN21AE3644 6307 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4117 278 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3650 1937 Size: 1713 4594 Acc65I G'GTAC_C Cuts at: 3628 3628 Size: 6307 AclI AA'CG_TT Cuts at: 1563 3036 4434 4807 1563 Size: 1473 1398 373 3063 Fragments arranged by size: 3063 1473 1398 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2283 2716 3222 3383 3573 4370 5754 6022 Size: 651 433 506 161 190 797 1384 268 Cuts at: 6022 1632 Size: 1917 Fragments arranged by size: 1917 1384 797 651 506 433 268 190 161 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6110 AflIII A'CryG_T Cuts at: 1203 5206 6183 1203 Size: 4003 977 1327 Fragments arranged by size: 4003 1327 977 AgeI A'CCGG_T Cuts at: 5138 5138 Size: 6307 AhdI GACnn_n'nnGTC Cuts at: 5036 5036 Size: 6307 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6307 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6307 AlwNI CAG_nnn'CTG Cuts at: 2132 4050 5622 2132 Size: 1918 1572 2817 Fragments arranged by size: 2817 1918 1572 ApaLI G'TGCA_C Cuts at: 4364 5520 4364 Size: 1156 5151 ApoI r'AATT_y Cuts at: 1493 2136 2276 2437 2754 2794 2942 2968 3446 Size: 643 140 161 317 40 148 26 478 Cuts at: 3446 3616 3842 3994 6103 1493 Size: 170 226 152 2109 1697 Fragments arranged by size: 2109 1697 643 478 317 226 170 161 152 148 140 40 26 AseI AT'TA_AT Cuts at: 160 1032 4861 160 Size: 872 3829 1606 Fragments arranged by size: 3829 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 AvaI C'yCGr_G Cuts at: 1870 1943 3632 1870 Size: 73 1689 4545 Fragments arranged by size: 4545 1689 73 BamHI G'GATC_C Cuts at: 3637 4004 3637 Size: 367 5940 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3115 3628 5083 618 Size: 325 217 627 1328 513 1455 1842 Fragments arranged by size: 1842 1455 1328 627 513 325 217 BanII G_rGCy'C Cuts at: 1391 2779 3626 1391 Size: 1388 847 4072 Fragments arranged by size: 4072 1388 847 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5336 BbvCI CC'TCA_GC Cuts at: 2054 2054 Size: 6307 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4498 4532 1903 Size: 34 2561 34 3678 Fragments arranged by size: 3678 2561 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3211 4199 5409 3211 Size: 988 1210 4109 Fragments arranged by size: 4109 1210 988 BclI T'GATC_A Cuts at: 1548 2068 2652 1548 Size: 520 584 5203 Fragments arranged by size: 5203 584 520 BfrBI ATG'CAT Cuts at: 2540 2540 Size: 6307 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4918 136 Size: 107 122 71 631 605 3246 1525 Fragments arranged by size: 3246 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6302 6302 Size: 6307 BlpI GC'TnA_GC Cuts at: 2419 3468 3724 2419 Size: 1049 256 5002 Fragments arranged by size: 5002 1049 256 Bme1580I G_kGCm'C Cuts at: 1790 4368 5524 1790 Size: 2578 1156 2573 Fragments arranged by size: 2578 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6307 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4038 4996 442 Size: 416 53 83 399 24 2621 958 1753 Fragments arranged by size: 2621 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6307 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2763 4967 1239 Size: 1524 2204 2579 Fragments arranged by size: 2579 2204 1524 Bpu10I CC'TnA_GC Cuts at: 2054 3196 2054 Size: 1142 5165 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2012 3495 4432 5297 5595 2012 Size: 1483 937 865 298 2724 Fragments arranged by size: 2724 1483 937 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4970 882 Size: 617 3471 2219 Fragments arranged by size: 3471 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4098 493 Size: 711 2894 2702 Fragments arranged by size: 2894 2702 711 BsaBI GATnn'nnATC Cuts at: 2190 2607 2651 3190 6301 2190 Size: 417 44 539 3111 2196 Fragments arranged by size: 3111 2196 539 417 44 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4114 4496 5131 275 Size: 53 83 186 936 2581 382 635 1451 Fragments arranged by size: 2581 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4740 5138 5412 5559 5970 4740 Size: 398 274 147 411 5077 Fragments arranged by size: 5077 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2024 2054 1178 Size: 30 165 30 621 30 5431 Fragments arranged by size: 5431 621 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6307 BseYI C'CCAG_C Cuts at: 2014 5510 6006 2014 Size: 3496 496 2315 Fragments arranged by size: 3496 2315 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5734 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2047 3757 4518 5546 1540 Size: 275 186 46 1710 761 1028 2301 Fragments arranged by size: 2301 1710 1028 761 275 186 46 BsiHKAI G_wGCw'C Cuts at: 4368 4453 5524 4368 Size: 85 1071 5151 Fragments arranged by size: 5151 1071 85 BsmI GAATG_Cn' Cuts at: 3488 3818 3911 3488 Size: 330 93 5884 Fragments arranged by size: 5884 330 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2779 3626 4368 4453 5524 1391 Size: 399 989 847 742 85 1071 2174 Fragments arranged by size: 2174 1071 989 847 742 399 85 BspEI T'CCGG_A Cuts at: 5970 5970 Size: 6307 BspHI T'CATG_A Cuts at: 4196 5926 4196 Size: 1730 4577 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3662 844 Size: 2818 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4194 1394 Size: 429 2371 3507 Fragments arranged by size: 3507 2371 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3480 4802 4976 65 Size: 1183 2232 1322 174 1396 Fragments arranged by size: 2232 1396 1322 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2046 4951 5138 1700 Size: 110 236 2905 187 2869 Fragments arranged by size: 2905 2869 236 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6307 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6307 BssSI C'ACGA_G Cuts at: 2928 4367 5379 2928 Size: 1439 1012 3856 Fragments arranged by size: 3856 1439 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6307 BstBI TT'CG_AA Cuts at: 5111 5111 Size: 6307 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6307 BstXI CCAn_nnnn'nTGG Cuts at: 2916 2916 Size: 6307 BstYI r'GATC_y Cuts at: 1972 2548 2844 3637 4004 4389 4406 5858 5944 Size: 576 296 793 367 385 17 1452 86 Cuts at: 5944 5956 6302 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 793 576 385 367 346 296 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2271 2642 513 Size: 552 939 267 371 4178 Fragments arranged by size: 4178 939 552 371 267 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2313 487 Size: 683 56 1087 4481 Fragments arranged by size: 4481 1087 683 56 BtsI GCAGTG_nn' Cuts at: 3823 4617 4637 3823 Size: 794 20 5493 Fragments arranged by size: 5493 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6307 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3431 3466 529 Size: 35 2867 35 3370 Fragments arranged by size: 3370 2867 35 35 DraI TTT'AAA Cuts at: 3611 3611 Size: 6307 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5314 809 Size: 507 3998 1802 Fragments arranged by size: 3998 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2949 3109 3754 Size: 54 1425 50 275 18 1119 160 645 Cuts at: 3754 4643 6272 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1119 889 645 275 160 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3754 1537 Size: 275 1942 4090 Fragments arranged by size: 4090 1942 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2316 2784 4237 1353 Size: 963 468 1453 3423 Fragments arranged by size: 3423 1453 963 468 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4894 5268 5414 387 Size: 1064 138 3305 374 146 1280 Fragments arranged by size: 3305 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 2716 2763 3222 3383 3573 4370 Size: 393 651 433 47 459 161 190 797 Cuts at: 4370 4967 5754 6022 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 797 787 651 597 459 433 393 268 190 161 47 EcoNI CCTnn'n_nnAGG Cuts at: 2979 2979 Size: 6307 EcoRI G'AATT_C Cuts at: 2276 2437 2754 3616 3994 2276 Size: 161 317 862 378 4589 Fragments arranged by size: 4589 862 378 317 161 EcoRV GAT'ATC Cuts at: 3329 3329 Size: 6307 FspI TGC'GCA Cuts at: 4813 4813 Size: 6307 HaeII r_GCGC'y Cuts at: 1678 5454 1678 Size: 3776 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2911 2943 4948 4980 5022 Size: 32 135 32 1339 32 2005 32 42 Cuts at: 5022 5054 1373 Size: 32 2626 Fragments arranged by size: 2626 2005 1339 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3651 3897 669 Size: 988 281 1713 246 3079 Fragments arranged by size: 3079 1713 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6307 HpaI GTT'AAC Cuts at: 3897 3897 Size: 6307 KpnI G_GTAC'C Cuts at: 3632 3632 Size: 6307 MfeI C'AATT_G Cuts at: 3906 3906 Size: 6307 MluI A'CGCG_T Cuts at: 6183 6183 Size: 6307 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5421 5605 1305 Size: 4116 184 2007 Fragments arranged by size: 4116 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2951 3111 10 Size: 54 1425 343 1119 160 3206 Fragments arranged by size: 3206 1425 1119 343 160 54 MslI CAynn'nnrTG Cuts at: 518 1166 4265 4624 4783 518 Size: 648 3099 359 159 2042 Fragments arranged by size: 3099 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2053 2202 3071 3723 4400 5548 5793 Size: 408 39 149 869 652 677 1148 245 Cuts at: 5793 1606 Size: 2120 Fragments arranged by size: 2120 1148 869 677 652 408 245 149 39 NcoI C'CATG_G Cuts at: 513 1065 2004 2271 2642 513 Size: 552 939 267 371 4178 Fragments arranged by size: 4178 939 552 371 267 NdeI CA'TA_TG Cuts at: 387 2470 2613 387 Size: 2083 143 4081 Fragments arranged by size: 4081 2083 143 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6307 NotI GC'GGCC_GC Cuts at: 3754 3754 Size: 6307 NsiI A_TGCA'T Cuts at: 2542 2542 Size: 6307 NspI r_CATG'y Cuts at: 1132 1229 3580 3665 5210 1132 Size: 97 2351 85 1545 2229 Fragments arranged by size: 2351 2229 1545 97 85 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6307 PciI A'CATG_T Cuts at: 5206 5206 Size: 6307 PflMI CCAn_nnn'nTGG Cuts at: 1108 2165 1108 Size: 1057 5250 PfoI T'CCnGG_A Cuts at: 3331 3331 Size: 6307 PmeI GTTT'AAAC Cuts at: 3611 3611 Size: 6307 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4376 4408 5889 5921 4376 Size: 32 1481 32 4762 Fragments arranged by size: 4762 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2631 1613 Size: 1018 5289 PsiI TTA'TAA Cuts at: 2501 3877 2501 Size: 1376 4931 PstI C_TGCA'G Cuts at: 830 3102 3158 3659 830 Size: 2272 56 501 3478 Fragments arranged by size: 3478 2272 501 56 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6307 PvuII CAG'CTG Cuts at: 2014 2053 2014 Size: 39 6268 SalI G'TCGA_C Cuts at: 1936 3649 1936 Size: 1713 4594 SbfI CC_TGCA'GG Cuts at: 3659 3659 Size: 6307 ScaI AGT'ACT Cuts at: 4555 6267 4555 Size: 1712 4595 SexAI A'CCwGG_T Cuts at: 2158 2158 Size: 6307 SfcI C'TryA_G Cuts at: 826 1045 2365 2461 3098 3154 3655 4790 5471 Size: 219 1320 96 637 56 501 1135 681 Cuts at: 5471 5662 826 Size: 191 1471 Fragments arranged by size: 1471 1320 1135 681 637 501 219 191 96 56 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6307 SmaI CCC'GGG Cuts at: 1872 3634 1872 Size: 1762 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2027 3510 4411 5312 5574 820 Size: 197 926 84 1483 901 901 262 1553 Fragments arranged by size: 1553 1483 926 901 901 262 197 84 SnaBI TAC'GTA Cuts at: 493 4098 493 Size: 3605 2702 SpeI A'CTAG_T Cuts at: 152 2476 152 Size: 2324 3983 SphI G_CATG'C Cuts at: 1132 3665 1132 Size: 2533 3774 SspI AAT'ATT Cuts at: 5 52 2666 4231 5 Size: 47 2614 1565 2081 Fragments arranged by size: 2614 2081 1565 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6307 StyI C'CwwG_G Cuts at: 513 1065 2004 2271 2642 3746 513 Size: 552 939 267 371 1104 3074 Fragments arranged by size: 3074 1104 939 552 371 267 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4334 4351 4504 4689 5134 325 Size: 1255 2754 17 153 185 445 1498 Fragments arranged by size: 2754 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3279 4553 6265 Size: 274 80 33 51 1443 1302 1274 1712 Cuts at: 6265 96 Size: 138 Fragments arranged by size: 1712 1443 1302 1274 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2301 2333 6267 6299 2301 Size: 32 3934 32 2309 Fragments arranged by size: 3934 2309 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6307 XbaI T'CTAG_A Cuts at: 2341 3643 2341 Size: 1302 5005 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6307 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6307 XmaI C'CCGG_G Cuts at: 1870 3632 1870 Size: 1762 4545 XmnI GAAnn'nnTTC Cuts at: 2337 2589 4436 2337 Size: 252 1847 4208 Fragments arranged by size: 4208 1847 252 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4115 276 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI