(Circular) (Six-base) MAPSORT of: pFN21AE2547.seq Check: 3242 from: 1 to: 6097 pFN21AE2547 6097 bp With 182 enzymes: SgfI * October 24, 2014 11:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3440 1937 Size: 1503 4594 Acc65I G'GTAC_C Cuts at: 3418 3418 Size: 6097 AclI AA'CG_TT Cuts at: 1563 4224 4597 1563 Size: 2661 373 3063 Fragments arranged by size: 3063 2661 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2135 2327 4160 5544 5812 1632 Size: 503 192 1833 1384 268 1917 Fragments arranged by size: 1917 1833 1384 503 268 192 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5900 AflIII A'CryG_T Cuts at: 1203 4996 5973 1203 Size: 3793 977 1327 Fragments arranged by size: 3793 1327 977 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6097 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6097 AlwNI CAG_nnn'CTG Cuts at: 3840 5412 3840 Size: 1572 4525 ApaLI G'TGCA_C Cuts at: 2667 4154 5310 2667 Size: 1487 1156 3454 Fragments arranged by size: 3454 1487 1156 ApoI r'AATT_y Cuts at: 1493 2057 2301 3406 3632 3784 5893 1493 Size: 564 244 1105 226 152 2109 1697 Fragments arranged by size: 2109 1697 1105 564 244 226 152 AseI AT'TA_AT Cuts at: 160 1032 4651 160 Size: 872 3619 1606 Fragments arranged by size: 3619 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 2255 3422 1870 Size: 73 312 1167 4545 Fragments arranged by size: 4545 1167 312 73 BamHI G'GATC_C Cuts at: 3290 3427 3794 3290 Size: 137 367 5593 Fragments arranged by size: 5593 367 137 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3418 4873 618 Size: 325 217 627 1631 1455 1842 Fragments arranged by size: 1842 1631 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2858 2917 3065 3416 1391 Size: 1467 59 148 351 4072 Fragments arranged by size: 4072 1467 351 148 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5126 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2369 2403 4288 4322 1903 Size: 34 432 34 1885 34 3678 Fragments arranged by size: 3678 1885 432 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3989 5199 3989 Size: 1210 4887 BclI T'GATC_A Cuts at: 1548 1548 Size: 6097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4708 136 Size: 107 122 71 631 605 3036 1525 Fragments arranged by size: 3036 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2108 6092 2108 Size: 3984 2113 BlpI GC'TnA_GC Cuts at: 3514 3514 Size: 6097 Bme1580I G_kGCm'C Cuts at: 1790 2671 4158 5314 1790 Size: 881 1487 1156 2573 Fragments arranged by size: 2573 1487 1156 881 BmgBI CAC'GTC Cuts at: 1318 2666 1318 Size: 1348 4749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2747 3828 4786 Size: 416 53 83 399 24 1330 1081 958 Cuts at: 4786 442 Size: 1753 Fragments arranged by size: 1753 1330 1081 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3012 4757 1239 Size: 1773 1745 2579 Fragments arranged by size: 2579 1773 1745 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4222 5087 5385 4222 Size: 865 298 4934 Fragments arranged by size: 4934 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2952 2980 2988 4760 882 Size: 617 1453 28 8 1772 2219 Fragments arranged by size: 2219 1772 1453 617 28 8 BsaAI yAC'GTr Cuts at: 493 1204 3888 493 Size: 711 2684 2702 Fragments arranged by size: 2702 2684 711 BsaBI GATnn'nnATC Cuts at: 6091 6091 Size: 6097 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2749 2815 3904 4286 Size: 53 83 186 936 1216 66 1089 382 Cuts at: 4286 4921 275 Size: 635 1451 Fragments arranged by size: 1451 1216 1089 936 635 382 186 83 66 53 BsaWI w'CCGG_w Cuts at: 3104 4530 4928 5202 5349 5760 3104 Size: 1426 398 274 147 411 3441 Fragments arranged by size: 3441 1426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5872 Fragments arranged by size: 5872 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2871 1186 Size: 1685 4412 BseYI C'CCAG_C Cuts at: 2165 5300 5796 2165 Size: 3135 496 2466 Fragments arranged by size: 3135 2466 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5524 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2899 3547 4308 5336 1540 Size: 275 186 898 648 761 1028 2301 Fragments arranged by size: 2301 1028 898 761 648 275 186 BsiHKAI G_wGCw'C Cuts at: 2671 2917 4158 4243 5314 2671 Size: 246 1241 85 1071 3454 Fragments arranged by size: 3454 1241 1071 246 85 BsmI GAATG_Cn' Cuts at: 2142 2167 2402 2558 2799 3346 3608 3701 2142 Size: 25 235 156 241 547 262 93 4538 Fragments arranged by size: 4538 547 262 241 235 156 93 25 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2671 2858 2917 3065 3416 4158 4243 Size: 399 881 187 59 148 351 742 85 Cuts at: 4243 5314 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 881 742 399 351 187 148 85 59 BspEI T'CCGG_A Cuts at: 3104 5760 3104 Size: 2656 3441 BspHI T'CATG_A Cuts at: 3247 3986 5716 3247 Size: 739 1730 3628 Fragments arranged by size: 3628 1730 739 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3452 844 Size: 2608 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2377 3984 1394 Size: 429 554 1607 3507 Fragments arranged by size: 3507 1607 554 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4592 4766 65 Size: 1183 3344 174 1396 Fragments arranged by size: 3344 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4741 4928 1700 Size: 110 2931 187 2869 Fragments arranged by size: 2931 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6097 BssSI C'ACGA_G Cuts at: 4157 5169 4157 Size: 1012 5085 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6097 BstBI TT'CG_AA Cuts at: 4901 4901 Size: 6097 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6097 BstXI CCAn_nnnn'nTGG Cuts at: 3072 3196 3072 Size: 124 5973 BstYI r'GATC_y Cuts at: 1972 2108 3203 3290 3427 3794 4179 4196 5648 Size: 136 1095 87 137 367 385 17 1452 Cuts at: 5648 5734 5746 6092 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1095 385 367 346 137 136 87 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4606 Fragments arranged by size: 4606 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5358 Fragments arranged by size: 5358 683 56 BtsI GCAGTG_nn' Cuts at: 2178 2254 2355 3613 4407 4427 2178 Size: 76 101 1258 794 20 3848 Fragments arranged by size: 3848 1258 794 101 76 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6062 DraI TTT'AAA Cuts at: 2104 3401 2104 Size: 1297 4800 DraIII CAC_nnn'GTG Cuts at: 3098 3098 Size: 6097 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5104 809 Size: 507 3788 1802 Fragments arranged by size: 3788 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2896 3544 4433 Size: 54 1425 50 275 18 1066 648 889 Cuts at: 4433 6062 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1066 889 648 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2896 3544 1537 Size: 275 1084 648 4090 Fragments arranged by size: 4090 1084 648 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2082 2325 2770 2830 2934 2974 4027 1353 Size: 729 243 445 60 104 40 1053 3423 Fragments arranged by size: 3423 1053 729 445 243 104 60 40 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4684 5058 5204 387 Size: 1064 138 3095 374 146 1280 Fragments arranged by size: 3095 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2915 2915 Size: 6097 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2327 3012 4160 4757 5544 5812 Size: 393 503 192 685 1148 597 787 268 Cuts at: 5812 1239 Size: 1524 Fragments arranged by size: 1524 1148 787 685 597 503 393 268 192 EcoNI CCTnn'n_nnAGG Cuts at: 2862 3285 2862 Size: 423 5674 EcoO109I rG'GnC_Cy Cuts at: 2335 2335 Size: 6097 EcoRI G'AATT_C Cuts at: 3406 3784 3406 Size: 378 5719 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 5244 1678 Size: 3566 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2627 2659 2845 2877 3249 Size: 32 135 32 1055 32 186 32 372 Cuts at: 3249 3281 4738 4770 4812 4844 1373 Size: 32 1457 32 42 32 2626 Fragments arranged by size: 2626 1457 1055 372 186 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3441 3687 669 Size: 988 281 1503 246 3079 Fragments arranged by size: 3079 1503 988 281 246 HindIII A'AGCT_T Cuts at: 748 2540 748 Size: 1792 4305 HpaI GTT'AAC Cuts at: 3687 3687 Size: 6097 KpnI G_GTAC'C Cuts at: 3422 3422 Size: 6097 MfeI C'AATT_G Cuts at: 2292 3696 2292 Size: 1404 4693 MluI A'CGCG_T Cuts at: 5973 5973 Size: 6097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5211 5395 1305 Size: 3906 184 2007 Fragments arranged by size: 3906 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4275 Fragments arranged by size: 4275 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3070 4055 4414 4573 518 Size: 648 1904 985 359 159 2042 Fragments arranged by size: 2042 1904 985 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3229 3513 4190 5338 5583 1606 Size: 1623 284 677 1148 245 2120 Fragments arranged by size: 2120 1623 1148 677 284 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4606 Fragments arranged by size: 4606 939 552 NdeI CA'TA_TG Cuts at: 387 3142 387 Size: 2755 3342 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 3544 3544 Size: 6097 NspI r_CATG'y Cuts at: 1132 1229 3455 5000 1132 Size: 97 2226 1545 2229 Fragments arranged by size: 2229 2226 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6097 PciI A'CATG_T Cuts at: 4996 4996 Size: 6097 PflMI CCAn_nnn'nTGG Cuts at: 1108 3098 1108 Size: 1990 4107 PfoI T'CCnGG_A Cuts at: 2547 2884 2547 Size: 337 5760 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4166 4198 5679 5711 4166 Size: 32 1481 32 4552 Fragments arranged by size: 4552 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6097 PsiI TTA'TAA Cuts at: 3667 3667 Size: 6097 PstI C_TGCA'G Cuts at: 830 2077 2343 3360 3449 830 Size: 1247 266 1017 89 3478 Fragments arranged by size: 3478 1247 1017 266 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 SacI G_AGCT'C Cuts at: 2917 2917 Size: 6097 SalI G'TCGA_C Cuts at: 1936 3439 1936 Size: 1503 4594 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 4345 6057 4345 Size: 1712 4385 SfcI C'TryA_G Cuts at: 826 1045 2073 2339 3356 3445 4580 5261 5452 Size: 219 1028 266 1017 89 1135 681 191 Cuts at: 5452 826 Size: 1471 Fragments arranged by size: 1471 1135 1028 1017 681 266 219 191 89 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 3424 1872 Size: 1552 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4201 5102 5364 820 Size: 197 926 2258 901 262 1553 Fragments arranged by size: 2258 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3888 493 Size: 3395 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 3455 1132 Size: 2323 3774 SspI AAT'ATT Cuts at: 5 52 2197 4021 5 Size: 47 2145 1824 2081 Fragments arranged by size: 2145 2081 1824 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6097 StyI C'CwwG_G Cuts at: 513 1065 2004 2325 3536 513 Size: 552 939 321 1211 3074 Fragments arranged by size: 3074 1211 939 552 321 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2368 4124 4141 4294 4479 4924 325 Size: 1255 788 1756 17 153 185 445 1498 Fragments arranged by size: 1756 1498 1255 788 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4343 6055 96 Size: 274 80 33 51 1443 2366 1712 138 Fragments arranged by size: 2366 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2739 2771 6057 6089 2739 Size: 32 3286 32 2747 Fragments arranged by size: 3286 2747 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6097 XbaI T'CTAG_A Cuts at: 2111 2831 3433 2111 Size: 720 602 4775 Fragments arranged by size: 4775 720 602 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6097 XmaI C'CCGG_G Cuts at: 1870 3422 1870 Size: 1552 4545 XmnI GAAnn'nnTTC Cuts at: 4226 4226 Size: 6097 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI