################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MslI MunI NarI NciI NcoI NdeI NheI NlaIV NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI TspRI Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccIII 149 TCCGGA T^CCGGA AcsI 211 AAATTC [GA]^AATT[CT] AflIII 262 ACACGT A^C[GA][CT]GT AlwI 138 GATCC GGATC(4/5) AocI 204 CCTCAGG CC^T[ACGT]AGG ApoI 211 AAATTC [GA]^AATT[CT] AvaI 17 CTCGAG C^[CT]CG[GA]G AvaII 243 GGACC G^G[AT]CC BalI 2 TGGCCA TGG^CCA BbrPI 263 CACGTG CAC^GTG BglII 21 AGATCT A^GATCT BsaAI 263 CACGTG [CT]AC^GT[GA] BsaJI 239 CCCTGG C^C[ACGT][ACGT]GG BsaWI 149 TCCGGA [AT]^CCGG[AT] BseAI 149 TCCGGA T^CCGGA BsiYI 69 CCTGAAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 69 CCTGAAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 84 GTCCC GGGAC(10/14) BsoBI 17 CTCGAG C^[CT]CG[GA]G BspEI 149 TCCGGA T^CCGGA BspMI 245 ACCTGC ACCTGC(4/8) BsrBI 331 GAGCGG CCGCTC(-3/-3) BsrDI 159 CATTGC GCAATG(2/0) BsrFI 278 GCCGGC [GA]^CCGG[CT] BssKI 240 CCTGG ^CC[ACGT]GG BssSI 292 CACGAG CACGAG(-5/-1) BstNI 240 CCTGG CC^[AT]GG Bsu36I 204 CCTCAGG CC^T[ACGT]AGG Cac8I 278 GCCGGC GC[ACGT]^[ACGT]GC Cfr10I 278 GCCGGC [GA]^CCGG[CT] Cfr13I 243 GGACC G^G[ACGT]CC EaeI 2 TGGCCA [CT]^GGCC[GA] Eco81I 204 CCTCAGG CC^T[ACGT]AGG EcoRII 240 CCTGG ^CC[AT]GG HgaI 95 GCGTC GACGC(5/10) MaeII 264 ACGT A^CGT MaeIII 106 GTGAC ^GT[ACGT]AC MluNI 2 TGGCCA TGG^CCA MroI 149 TCCGGA T^CCGGA MscI 2 TGGCCA TGG^CCA MseI 182 TTAA T^TAA MvaI 240 CCTGG CC^[AT]GG NaeI 278 GCCGGC GCC^GGC NgoMIV 278 GCCGGC G^CCGGC NlaIII 270 CATG CATG^ PaeR7I 17 CTCGAG C^TCGAG PmaCI 263 CACGTG CAC^GTG PmlI 263 CACGTG CAC^GTG Sau96I 243 GGACC G^G[ACGT]CC ScrFI 240 CCTGG CC^[ACGT]GG SmlI 17 CTCGAG C^T[CT][GA]AG TaqI 18 TCGA T^CGA Tru9I 182 TTAA T^TAA Tsp45I 106 GTGAC ^GT[GC]AC Tsp509I 212 AATT ^AATT TthHB8I 18 TCGA T^CGA XhoI 17 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BbsI 258 GAAGAC GAAGAC(2/6) 306 GAAGAC BpuAI 258 GAAGAC GAAGAC(2/6) 306 GAAGAC BseRI 43 GAGGAG GAGGAG(10/8) 295 GAGGAG BstYI 21 AGATCT [GA]^GATC[CT] 137 AGATCC CfoI 93 GCGC GCG^C 273 GCGC FauI 342 CCCGC CCCGC(4/6) 29 GCGGG HapII 150 CCGG C^CGG 279 CCGG HhaI 93 GCGC GCG^C 273 GCGC HinP1I 93 GCGC G^CGC 273 GCGC HinfI 143 GAGTC G^A[ACGT]TC 189 GACTC HpaII 150 CCGG C^CGG 279 CCGG MflI 21 AGATCT [GA]^GATC[CT] 137 AGATCC MspI 150 CCGG C^CGG 279 CCGG PleI 189 GACTC GAGTC(4/5) 143 GAGTC PvuII 58 CAGCTG CAG^CTG 88 CAGCTG XhoII 21 AGATCT [GA]^GATC[CT] 137 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AccII AciI AfaI AluI BbvI BstUI DdeI DpnII Fnu4HI HaeIII ItaI MboI MboII MnlI MspA1I MvnI MwoI NdeII RsaI Sau3AI TseI