(Circular) (Six-base) MAPSORT of: pFN21AE5121.seq Check: 4484 from: 1 to: 5074 pFN21AE5121 5074 bp With 182 enzymes: SgfI * January 30, 2014 17:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2884 278 Size: 53 83 186 936 1348 2468 Fragments arranged by size: 2468 1348 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2417 1937 Size: 480 4594 Acc65I G'GTAC_C Cuts at: 2395 2395 Size: 5074 AclI AA'CG_TT Cuts at: 1563 3201 3574 1563 Size: 1638 373 3063 Fragments arranged by size: 3063 1638 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2330 3137 4521 4789 1632 Size: 464 234 807 1384 268 1917 Fragments arranged by size: 1917 1384 807 464 268 234 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4877 AflIII A'CryG_T Cuts at: 1203 2267 3973 4950 1203 Size: 1064 1706 977 1327 Fragments arranged by size: 1706 1327 1064 977 AgeI A'CCGG_T Cuts at: 3905 3905 Size: 5074 AhdI GACnn_n'nnGTC Cuts at: 3803 3803 Size: 5074 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5074 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5074 AlwNI CAG_nnn'CTG Cuts at: 2817 4389 2817 Size: 1572 3502 ApaLI G'TGCA_C Cuts at: 3131 4287 3131 Size: 1156 3918 ApoI r'AATT_y Cuts at: 1493 2216 2383 2609 2761 4870 1493 Size: 723 167 226 152 2109 1697 Fragments arranged by size: 2109 1697 723 226 167 152 AseI AT'TA_AT Cuts at: 160 1032 3628 160 Size: 872 2596 1606 Fragments arranged by size: 2596 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5074 AvaI C'yCGr_G Cuts at: 1870 1943 2022 2399 1870 Size: 73 79 377 4545 Fragments arranged by size: 4545 377 79 73 BamHI G'GATC_C Cuts at: 2404 2771 2404 Size: 367 4707 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2395 3850 618 Size: 325 217 627 608 1455 1842 Fragments arranged by size: 1842 1455 627 608 325 217 BanII G_rGCy'C Cuts at: 1391 2393 1391 Size: 1002 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2270 2318 928 Size: 971 371 48 3684 Fragments arranged by size: 3684 971 371 48 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3265 3299 1903 Size: 34 1328 34 3678 Fragments arranged by size: 3678 1328 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2966 4176 2966 Size: 1210 3864 BclI T'GATC_A Cuts at: 1548 1548 Size: 5074 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3685 136 Size: 107 122 71 631 605 2013 1525 Fragments arranged by size: 2013 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2026 5069 2026 Size: 3043 2031 BlpI GC'TnA_GC Cuts at: 2491 2491 Size: 5074 Bme1580I G_kGCm'C Cuts at: 1790 3135 4291 1790 Size: 1345 1156 2573 Fragments arranged by size: 2573 1345 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5074 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2805 3763 442 Size: 416 53 83 399 24 1388 958 1753 Fragments arranged by size: 1753 1388 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5074 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3734 1239 Size: 2495 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3199 4064 4362 3199 Size: 865 298 3911 Fragments arranged by size: 3911 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3737 882 Size: 617 2238 2219 Fragments arranged by size: 2238 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2270 2865 493 Size: 711 1066 595 2702 Fragments arranged by size: 2702 1066 711 595 BsaBI GATnn'nnATC Cuts at: 5068 5068 Size: 5074 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2881 3263 3898 275 Size: 53 83 186 936 1348 382 635 1451 Fragments arranged by size: 1451 1348 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2154 3507 3905 4179 4326 4737 2154 Size: 1353 398 274 147 411 2491 Fragments arranged by size: 2491 1353 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2327 2357 1178 Size: 30 165 30 924 30 3895 Fragments arranged by size: 3895 924 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2315 1186 Size: 877 252 3945 Fragments arranged by size: 3945 877 252 BseYI C'CCAG_C Cuts at: 2091 4277 4773 2091 Size: 2186 496 2392 Fragments arranged by size: 2392 2186 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4501 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2524 3285 4313 1540 Size: 275 186 523 761 1028 2301 Fragments arranged by size: 2301 1028 761 523 275 186 BsiHKAI G_wGCw'C Cuts at: 3135 3220 4291 3135 Size: 85 1071 3918 Fragments arranged by size: 3918 1071 85 BsmI GAATG_Cn' Cuts at: 2585 2678 2585 Size: 93 4981 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2393 3135 3220 4291 1391 Size: 399 603 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 603 399 85 BspEI T'CCGG_A Cuts at: 2154 4737 2154 Size: 2583 2491 BspHI T'CATG_A Cuts at: 2963 4693 2963 Size: 1730 3344 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2259 2429 844 Size: 1415 170 3489 Fragments arranged by size: 3489 1415 170 BsrBI CCG'CTC Cuts at: 1394 1823 2338 2961 1394 Size: 429 515 623 3507 Fragments arranged by size: 3507 623 515 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2163 3569 3743 65 Size: 1183 915 1406 174 1396 Fragments arranged by size: 1406 1396 1183 915 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2283 3718 3905 1700 Size: 110 473 1435 187 2869 Fragments arranged by size: 2869 1435 473 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5074 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5074 BssSI C'ACGA_G Cuts at: 2297 3134 4146 2297 Size: 837 1012 3225 Fragments arranged by size: 3225 1012 837 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5074 BstBI TT'CG_AA Cuts at: 3878 3878 Size: 5074 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5074 BstYI r'GATC_y Cuts at: 1972 2026 2142 2404 2771 3156 3173 4625 4711 Size: 54 116 262 367 385 17 1452 86 Cuts at: 4711 4723 5069 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 262 116 86 54 17 12 Bsu36I CC'TnA_GG Cuts at: 2210 2210 Size: 5074 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3583 Fragments arranged by size: 3583 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4335 Fragments arranged by size: 4335 683 56 BtsI GCAGTG_nn' Cuts at: 2590 3384 3404 2590 Size: 794 20 4260 Fragments arranged by size: 4260 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5074 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5039 DraI TTT'AAA Cuts at: 2378 2378 Size: 5074 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4081 809 Size: 507 2765 1802 Fragments arranged by size: 2765 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2521 3410 Size: 54 1425 50 275 18 177 514 889 Cuts at: 3410 5039 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 514 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2521 1537 Size: 275 709 4090 Fragments arranged by size: 4090 709 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3004 1353 Size: 1651 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3661 4035 4181 387 Size: 1064 138 2072 374 146 1280 Fragments arranged by size: 2072 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2330 3137 3734 4521 4789 1239 Size: 393 464 234 807 597 787 268 1524 Fragments arranged by size: 1524 807 787 597 464 393 268 234 EcoRI G'AATT_C Cuts at: 2383 2761 2383 Size: 378 4696 FspI TGC'GCA Cuts at: 3580 3580 Size: 5074 HaeII r_GCGC'y Cuts at: 1678 4221 1678 Size: 2543 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2005 2037 3715 3747 3789 Size: 32 135 32 433 32 1678 32 42 Cuts at: 3789 3821 1373 Size: 32 2626 Fragments arranged by size: 2626 1678 433 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2418 2664 669 Size: 988 281 480 246 3079 Fragments arranged by size: 3079 988 480 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5074 HpaI GTT'AAC Cuts at: 2664 2664 Size: 5074 KpnI G_GTAC'C Cuts at: 2399 2399 Size: 5074 MfeI C'AATT_G Cuts at: 2673 2673 Size: 5074 MluI A'CGCG_T Cuts at: 4950 4950 Size: 5074 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4188 4372 1305 Size: 2883 184 2007 Fragments arranged by size: 2883 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3075 Fragments arranged by size: 3075 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3032 3391 3550 518 Size: 648 1866 359 159 2042 Fragments arranged by size: 2042 1866 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2065 2095 2329 2350 2490 3167 4315 4560 Size: 459 30 234 21 140 677 1148 245 Cuts at: 4560 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 459 245 234 140 30 21 NaeI GCC'GGC Cuts at: 2285 2285 Size: 5074 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3583 Fragments arranged by size: 3583 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5074 NgoMIV G'CCGG_C Cuts at: 2283 2283 Size: 5074 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5074 NotI GC'GGCC_GC Cuts at: 2521 2521 Size: 5074 NspI r_CATG'y Cuts at: 1132 1229 2432 3977 1132 Size: 97 1203 1545 2229 Fragments arranged by size: 2229 1545 1203 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5074 PciI A'CATG_T Cuts at: 3973 3973 Size: 5074 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5074 PmeI GTTT'AAAC Cuts at: 2378 2378 Size: 5074 PmlI CAC'GTG Cuts at: 2270 2270 Size: 5074 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3143 3175 4656 4688 3143 Size: 32 1481 32 3529 Fragments arranged by size: 3529 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5074 PsiI TTA'TAA Cuts at: 2644 2644 Size: 5074 PstI C_TGCA'G Cuts at: 830 2426 830 Size: 1596 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5074 PvuII CAG'CTG Cuts at: 2065 2095 2065 Size: 30 5044 SalI G'TCGA_C Cuts at: 1936 2416 1936 Size: 480 4594 SbfI CC_TGCA'GG Cuts at: 2426 2426 Size: 5074 ScaI AGT'ACT Cuts at: 3322 5034 3322 Size: 1712 3362 SfcI C'TryA_G Cuts at: 826 1045 2422 3557 4238 4429 826 Size: 219 1377 1135 681 191 1471 Fragments arranged by size: 1471 1377 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5074 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5074 SmaI CCC'GGG Cuts at: 1872 2401 1872 Size: 529 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2022 3178 4079 4341 820 Size: 197 926 79 1156 901 262 1553 Fragments arranged by size: 1553 1156 926 901 262 197 79 SnaBI TAC'GTA Cuts at: 493 2865 493 Size: 2372 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5074 SphI G_CATG'C Cuts at: 1132 2432 1132 Size: 1300 3774 SspI AAT'ATT Cuts at: 5 52 2998 5 Size: 47 2946 2081 Fragments arranged by size: 2946 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5074 StyI C'CwwG_G Cuts at: 513 1065 2004 2513 513 Size: 552 939 509 3074 Fragments arranged by size: 3074 939 552 509 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3101 3118 3271 3456 3901 325 Size: 1255 1521 17 153 185 445 1498 Fragments arranged by size: 1521 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2235 3320 5032 Size: 274 80 33 51 1443 258 1085 1712 Cuts at: 5032 96 Size: 138 Fragments arranged by size: 1712 1443 1085 274 258 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5034 5066 5034 Size: 32 5042 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5074 XbaI T'CTAG_A Cuts at: 2410 2410 Size: 5074 XhoI C'TCGA_G Cuts at: 1943 2022 1943 Size: 79 4995 XmaI C'CCGG_G Cuts at: 1870 2399 1870 Size: 529 4545 XmnI GAAnn'nnTTC Cuts at: 3203 3203 Size: 5074 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2882 276 Size: 53 83 186 936 1348 2468 Fragments arranged by size: 2468 1348 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI