################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI KspI MamI MfeI MflI MluI MroI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TseI TspRI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 328 GTAGAC GT^[AC][GT]AC AciI 79 CCGC CCGC(-3/-1) AfaI 531 GTAC GT^AC AflIII 437 ACACGT A^C[GA][CT]GT AluI 258 AGCT AG^CT AlwI 413 GGATC GGATC(4/5) AlwNI 142 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 214 ATTAAT AT^TAAT AsnI 214 ATTAAT AT^TAAT Asp700I 87 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 257 GAGCTC G[AT]GC[AT]^C AspI 331 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 63 GGACC G^G[AT]CC BalI 73 TGGCCA TGG^CCA BanII 257 GAGCTC G[GA]GC[CT]^C BbsI 165 GAAGAC GAAGAC(2/6) BfaI 280 CTAG C^TAG BmyI 257 GAGCTC G[AGT]GC[ACT]^C BpuAI 165 GAAGAC GAAGAC(2/6) BseRI 514 GAGGAG GAGGAG(10/8) BsiHKAI 257 GAGCTC G[AT]GC[AT]^C Bsp1286I 257 GAGCTC G[AGT]GC[ACT]^C BspMI 65 ACCTGC ACCTGC(4/8) BsrBI 79 CCGCTC CCGCTC(-3/-3) Cfr13I 63 GGACC G^G[ACGT]CC DpnII 414 GATC ^GATC DraI 201 TTTAAA TTT^AAA DraII 62 GGGACCT [GA]G^G[ACGT]CC[CT] EaeI 73 TGGCCA [CT]^GGCC[GA] EarI 123 CTCTTC CTCTTC(1/4) EcoNI 247 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 62 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 91 GAATTC G^AATTC Ksp632I 123 CTCTTC CTCTTC(1/4) MaeI 280 CTAG C^TAG MaeII 439 ACGT A^CGT MboI 414 GATC ^GATC MluNI 73 TGGCCA TGG^CCA MscI 73 TGGCCA TGG^CCA NdeII 414 GATC ^GATC PflFI 331 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 36 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 62 GGGACCT [GA]G^G[AT]CC[CT] PshBI 214 ATTAAT AT^TAAT RsaI 531 GTAC GT^AC SacI 257 GAGCTC GAGCT^C Sau3AI 414 GATC ^GATC Sau96I 63 GGACC G^G[ACGT]CC SexAI 169 ACCTGGT A^CC[AT]GGT SmlI 238 CTCAAG C^T[CT][GA]AG TaqI 195 TCGA T^CGA Tth111I 331 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 195 TCGA T^CGA Van91I 36 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 279 TCTAGA T^CTAGA XmnI 87 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AcsI 91 GAATTC [GA]^AATT[CT] 535 AAATTT ApoI 91 GAATTC [GA]^AATT[CT] 535 AAATTT BsmFI 62 GGGAC GGGAC(10/14) 33 GTCCC HaeIII 74 GGCC GG^CC 388 GGCC HphI 150 GGTGA GGTGA(8/7) 381 TCACC MaeIII 406 GTGAC ^GT[ACGT]AC 434 GTGAC NlaIV 62 GGGACC GG[ACGT]^[ACGT]CC 243 GGAACC Tsp45I 406 GTGAC ^GT[GC]AC 434 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### BsaJI BsiYI BslI BssKI BstNI DdeI EcoRII HinfI MboII MnlI MseI MvaI NlaIII ScrFI SfaNI TfiI Tru9I Tsp509I