(Linear) (Six-base) MAPSORT of: KIEE1574.seq Check: 5739 from: 1 to: 2049 KIEE1574.orf With 182 enzymes: SgfI * July 15, 2010 18:42 .. AccI GT'mk_AC Cuts at: 0 1607 2049 Size: 1607 442 Acc65I G'GTAC_C Cuts at: 0 203 2049 Size: 203 1846 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 479 974 1546 2049 Size: 479 495 572 503 Fragments arranged by size: 572 503 495 479 AflIII A'CryG_T Cuts at: 0 140 2049 Size: 140 1909 AleI CACnn'nnGTG Cuts at: 0 1718 2049 Size: 1718 331 AlwNI CAG_nnn'CTG Cuts at: 0 1156 1362 1945 2049 Size: 1156 206 583 104 Fragments arranged by size: 1156 583 206 104 ApaI G_GGCC'C Cuts at: 0 52 404 1755 2049 Size: 52 352 1351 294 Fragments arranged by size: 1351 352 294 52 ApaLI G'TGCA_C Cuts at: 0 685 2049 Size: 685 1364 ApoI r'AATT_y Cuts at: 0 588 1248 1296 2049 Size: 588 660 48 753 Fragments arranged by size: 753 660 588 48 BamHI G'GATC_C Cuts at: 0 340 2049 Size: 340 1709 BanI G'GyrC_C Cuts at: 0 203 2049 Size: 203 1846 BanII G_rGCy'C Cuts at: 0 52 404 794 1647 1755 2049 Size: 52 352 390 853 108 294 Fragments arranged by size: 853 390 352 294 108 52 BbsI GAAGACnn'nnnn_ Cuts at: 0 1608 2049 Size: 1608 441 BbvCI CC'TCA_GC Cuts at: 0 382 2049 Size: 382 1667 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 649 683 2049 Size: 649 34 1366 Fragments arranged by size: 1366 649 34 BciVI GTATCCnnnnn_n' Cuts at: 0 895 2049 Size: 895 1154 BglI GCCn_nnn'nGGC Cuts at: 0 131 2049 Size: 131 1918 BglII A'GATC_T Cuts at: 0 1955 2049 Size: 1955 94 Bme1580I G_kGCm'C Cuts at: 0 52 404 645 689 997 1755 2049 Size: 52 352 241 44 308 758 294 Fragments arranged by size: 758 352 308 294 241 52 44 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1009 1024 1886 2049 Size: 1009 15 862 163 Fragments arranged by size: 1009 862 163 15 Bpu10I CC'TnA_GC Cuts at: 0 382 2049 Size: 382 1667 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1972 2049 Size: 1972 77 BsaI GGTCTCn'nnnn_ Cuts at: 0 316 754 905 976 1579 2049 Size: 316 438 151 71 603 470 Fragments arranged by size: 603 470 438 316 151 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 2049 Size: 172 1877 BsiHKAI G_wGCw'C Cuts at: 0 689 2049 Size: 689 1360 Bsp1286I G_dGCh'C Cuts at: 0 52 404 645 689 794 997 1647 1755 Size: 52 352 241 44 105 203 650 108 Cuts at: 1755 2049 Size: 294 Fragments arranged by size: 650 352 294 241 203 108 105 52 44 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 923 2049 Size: 923 1126 BsrBI CCG'CTC Cuts at: 0 1224 2049 Size: 1224 825 BsrDI GCAATG_nn' Cuts at: 0 489 2049 Size: 489 1560 BsrFI r'CCGG_y Cuts at: 0 1192 2049 Size: 1192 857 BsrGI T'GTAC_A Cuts at: 0 359 2049 Size: 359 1690 BstAPI GCAn_nnn'nTGC Cuts at: 0 1945 2049 Size: 1945 104 BstXI CCAn_nnnn'nTGG Cuts at: 0 681 2049 Size: 681 1368 BstYI r'GATC_y Cuts at: 0 340 602 860 1955 2049 Size: 340 262 258 1095 94 Fragments arranged by size: 1095 340 262 258 94 Bsu36I CC'TnA_GG Cuts at: 0 1916 2049 Size: 1916 133 BtgI C'CryG_G Cuts at: 0 1350 2049 Size: 1350 699 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1419 2049 Size: 1419 630 BtsI GCAGTG_nn' Cuts at: 0 205 2049 Size: 205 1844 ClaI AT'CG_AT Cuts at: 0 695 2049 Size: 695 1354 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1155 1190 1850 1885 2049 Size: 1155 35 660 35 164 Fragments arranged by size: 1155 660 164 35 35 DrdI GACnn_nn'nnGTC Cuts at: 0 475 2049 Size: 475 1574 EaeI y'GGCC_r Cuts at: 0 816 1856 2049 Size: 816 1040 193 Fragments arranged by size: 1040 816 193 EarI CTCTTCn'nnn_ Cuts at: 0 13 2049 Size: 13 2036 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 479 974 1009 1024 1546 1886 2049 Size: 479 495 35 15 522 340 163 Fragments arranged by size: 522 495 479 340 163 35 15 EcoO109I rG'GnC_Cy Cuts at: 0 49 70 270 400 1466 1751 1752 2049 Size: 49 21 200 130 1066 285 1 297 Fragments arranged by size: 1066 297 285 200 130 49 21 1 EcoRI G'AATT_C Cuts at: 0 588 1248 2049 Size: 588 660 801 Fragments arranged by size: 801 660 588 HaeII r_GCGC'y Cuts at: 0 7 2049 Size: 7 2042 HincII GTy'rAC Cuts at: 0 489 1229 1824 2049 Size: 489 740 595 225 Fragments arranged by size: 740 595 489 225 KpnI G_GTAC'C Cuts at: 0 207 2049 Size: 207 1842 MfeI C'AATT_G Cuts at: 0 1277 2049 Size: 1277 772 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 318 617 2049 Size: 318 299 1432 Fragments arranged by size: 1432 318 299 MscI TGG'CCA Cuts at: 0 1858 2049 Size: 1858 191 MslI CAynn'nnrTG Cuts at: 0 577 1718 2049 Size: 577 1141 331 Fragments arranged by size: 1141 577 331 MspA1I CmG'CkG Cuts at: 0 93 357 457 1019 1329 1430 2049 Size: 93 264 100 562 310 101 619 Fragments arranged by size: 619 562 310 264 101 100 93 NaeI GCC'GGC Cuts at: 0 1194 2049 Size: 1194 855 NcoI C'CATG_G Cuts at: 0 1350 2049 Size: 1350 699 NdeI CA'TA_TG Cuts at: 0 521 2049 Size: 521 1528 NgoMIV G'CCGG_C Cuts at: 0 1192 2049 Size: 1192 857 NspI r_CATG'y Cuts at: 0 1029 1551 2049 Size: 1029 522 498 Fragments arranged by size: 1029 522 498 PasI CC'CwG_GG Cuts at: 0 45 274 327 2049 Size: 45 229 53 1722 Fragments arranged by size: 1722 229 53 45 PflMI CCAn_nnn'nTGG Cuts at: 0 525 2049 Size: 525 1524 PfoI T'CCnGG_A Cuts at: 0 255 2049 Size: 255 1794 PpuMI rG'GwC_Cy Cuts at: 0 70 270 1466 2049 Size: 70 200 1196 583 Fragments arranged by size: 1196 583 200 70 PspOMI G'GGCC_C Cuts at: 0 48 400 1751 2049 Size: 48 352 1351 298 Fragments arranged by size: 1351 352 298 48 PstI C_TGCA'G Cuts at: 0 104 1018 1152 1575 1898 2049 Size: 104 914 134 423 323 151 Fragments arranged by size: 914 423 323 151 134 104 PvuII CAG'CTG Cuts at: 0 93 357 457 1329 1430 2049 Size: 93 264 100 872 101 619 Fragments arranged by size: 872 619 264 101 100 93 RsrII CG'GwC_CG Cuts at: 0 366 2049 Size: 366 1683 SanDI GG'GwC_CC Cuts at: 0 70 1466 2049 Size: 70 1396 583 Fragments arranged by size: 1396 583 70 SapI GCTCTTCn'nnn_ Cuts at: 0 13 2049 Size: 13 2036 ScaI AGT'ACT Cuts at: 0 181 2049 Size: 181 1868 SfcI C'TryA_G Cuts at: 0 100 1014 1148 1571 1894 2049 Size: 100 914 134 423 323 155 Fragments arranged by size: 914 423 323 155 134 100 SmlI C'TyrA_G Cuts at: 0 1951 2049 Size: 1951 98 SphI G_CATG'C Cuts at: 0 1551 2049 Size: 1551 498 StuI AGG'CCT Cuts at: 0 387 439 850 2049 Size: 387 52 411 1199 Fragments arranged by size: 1199 411 387 52 StyI C'CwwG_G Cuts at: 0 1350 1685 1776 2049 Size: 1350 335 91 273 Fragments arranged by size: 1350 335 273 91 TatI w'GTAC_w Cuts at: 0 179 359 821 2049 Size: 179 180 462 1228 Fragments arranged by size: 1228 462 180 179 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 204 236 2049 Size: 204 32 1813 Fragments arranged by size: 1813 204 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 616 1866 2049 Size: 616 1250 183 Fragments arranged by size: 1250 616 183 XmnI GAAnn'nnTTC Cuts at: 0 181 2049 Size: 181 1868 Enzymes that do cut: AccI Acc65I AcuI AflIII AleI AlwNI ApaI ApaLI ApoI BamHI BanI BanII BbsI BbvCI BcgI BciVI BglI BglII Bme1580I BpmI Bpu10I BpuEI BsaI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DrdI EaeI EarI Eco57MI EcoO109I EcoRI HaeII HincII KpnI MfeI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII RsrII SanDI SapI ScaI SfcI SmlI SphI StuI StyI TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AgeI AhdI AlfI AloI AscI AseI AsiSI AvaI AvrII BaeI BbeI BclI BfrBI BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BssHII BssSI BstBI BstEII BstZ17I DraI DraIII EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI KasI MluI NarI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI SacI SacII SalI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI