################################## # NO CUT ENZYMES ( 210 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FokI FseI FspI HapII HgaI HincII HindII HindIII HpaI HpaII HphI KpnI KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI MvaI NaeI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccII 65 CGCG CG^CG AcyI 31 GGCGCC G[GA]^CG[CT]C AlwNI 260 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG BanI 31 GGCGCC G^G[CT][GA]CC BanII 46 GAGCCC G[GA]GC[CT]^C BbeI 31 GGCGCC GGCGC^C BglI 187 GCCGAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 46 GAGCCC G[AGT]GC[ACT]^C BsaHI 31 GGCGCC G[GA]^CG[CT]C BsaJI 38 CCAAGG C^C[ACGT][ACGT]GG Bsp1286I 46 GAGCCC G[AGT]GC[ACT]^C BspMI 222 ACCTGC ACCTGC(4/8) BstUI 65 CGCG CG^CG DdeI 248 CTGAG C^T[ACGT]AG DpnII 59 GATC ^GATC EarI 43 GAAGAG CTCTTC(1/4) EcoT14I 38 CCAAGG C^C[AT][AT]GG HaeII 31 GGCGCC [GA]GCGC^[CT] HaeIII 186 GGCC GG^CC Hin1I 31 GGCGCC G[GA]^CG[CT]C HinfI 255 GAGTC G^A[ACGT]TC KasI 31 GGCGCC G^GCGCC Ksp632I 43 GAAGAG CTCTTC(1/4) MboI 59 GATC ^GATC MvnI 65 CGCG CG^CG NarI 31 GGCGCC GG^CGCC NdeII 59 GATC ^GATC NlaIV 31 GGCGCC GG[ACGT]^[ACGT]CC PleI 255 GAGTC GAGTC(4/5) SapI 43 GAAGAGC GCTCTTC(1/4) Sau3AI 59 GATC ^GATC SfoI 31 GGCGCC GGC^GCC StyI 38 CCAAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AluI 19 AGCT AG^CT 75 AGCT BbsI 214 GAAGAC GAAGAC(2/6) 243 GAAGAC BbvI 115 GCAGC GCAGC(8/12) 118 GCAGC BpuAI 214 GAAGAC GAAGAC(2/6) 243 GAAGAC BseRI 54 GAGGAG GAGGAG(10/8) 250 GAGGAG CfoI 32 GCGC GCG^C 64 GCGC HhaI 32 GCGC GCG^C 64 GCGC HinP1I 32 GCGC G^CGC 64 GCGC MwoI 25 GCCGAAGGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 187 GCCGAAGTGGC TseI 115 GCAGC G^C[AT]GC 118 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 5 enzymes) # ######################################### AciI Fnu4HI ItaI MboII MnlI