(Circular) (Six-base) MAPSORT of: pFN21AE6304.seq Check: 833 from: 1 to: 6034 pFN21AE6304 6034 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3267 3296 3267 Size: 29 6005 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3844 278 Size: 53 83 186 936 2308 2468 Fragments arranged by size: 2468 2308 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2813 3377 1937 Size: 876 564 4594 Fragments arranged by size: 4594 876 564 Acc65I G'GTAC_C Cuts at: 3355 3355 Size: 6034 AclI AA'CG_TT Cuts at: 1563 4161 4534 1563 Size: 2598 373 3063 Fragments arranged by size: 3063 2598 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2253 4097 5481 5749 1632 Size: 621 1844 1384 268 1917 Fragments arranged by size: 1917 1844 1384 621 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5837 AflIII A'CryG_T Cuts at: 1203 4933 5910 1203 Size: 3730 977 1327 Fragments arranged by size: 3730 1327 977 AgeI A'CCGG_T Cuts at: 4865 4865 Size: 6034 AhdI GACnn_n'nnGTC Cuts at: 4763 4763 Size: 6034 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6034 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6034 AlwNI CAG_nnn'CTG Cuts at: 3119 3777 5349 3119 Size: 658 1572 3804 Fragments arranged by size: 3804 1572 658 ApaI G_GGCC'C Cuts at: 2552 3324 2552 Size: 772 5262 ApaLI G'TGCA_C Cuts at: 4091 5247 4091 Size: 1156 4878 ApoI r'AATT_y Cuts at: 1493 2469 3343 3569 3721 5830 1493 Size: 976 874 226 152 2109 1697 Fragments arranged by size: 2109 1697 976 874 226 152 AseI AT'TA_AT Cuts at: 160 1032 4588 160 Size: 872 3556 1606 Fragments arranged by size: 3556 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 AvaI C'yCGr_G Cuts at: 1870 1943 3018 3052 3359 1870 Size: 73 1075 34 307 4545 Fragments arranged by size: 4545 1075 307 73 34 BamHI G'GATC_C Cuts at: 3364 3731 3364 Size: 367 5667 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2638 3255 3355 4810 618 Size: 325 217 627 851 617 100 1455 1842 Fragments arranged by size: 1842 1455 851 627 617 325 217 100 BanII G_rGCy'C Cuts at: 1391 2552 2788 2796 3227 3324 3353 1391 Size: 1161 236 8 431 97 29 4072 Fragments arranged by size: 4072 1161 431 236 97 29 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2394 3015 928 Size: 971 495 621 3947 Fragments arranged by size: 3947 971 621 495 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4225 4259 1903 Size: 34 2288 34 3678 Fragments arranged by size: 3678 2288 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3926 5136 3926 Size: 1210 4824 BclI T'GATC_A Cuts at: 1548 1548 Size: 6034 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4645 136 Size: 107 122 71 631 605 2973 1525 Fragments arranged by size: 2973 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6029 6029 Size: 6034 BlpI GC'TnA_GC Cuts at: 3451 3451 Size: 6034 Bme1580I G_kGCm'C Cuts at: 1790 2552 3324 4095 5251 1790 Size: 762 772 771 1156 2573 Fragments arranged by size: 2573 1156 772 771 762 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6034 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3765 4723 442 Size: 416 53 83 399 24 2348 958 1753 Fragments arranged by size: 2348 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2711 1056 Size: 1655 4379 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2392 2972 2392 Size: 580 5454 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4694 1239 Size: 3455 2579 Bpu10I CC'TnA_GC Cuts at: 2990 2990 Size: 6034 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4159 5024 5322 4159 Size: 865 298 4871 Fragments arranged by size: 4871 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2296 4697 882 Size: 617 797 2401 2219 Fragments arranged by size: 2401 2219 797 617 BsaAI yAC'GTr Cuts at: 493 1204 3205 3825 493 Size: 711 2001 620 2702 Fragments arranged by size: 2702 2001 711 620 BsaBI GATnn'nnATC Cuts at: 6028 6028 Size: 6034 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3841 4223 4858 275 Size: 53 83 186 936 2308 382 635 1451 Fragments arranged by size: 2308 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4467 4865 5139 5286 5697 4467 Size: 398 274 147 411 4804 Fragments arranged by size: 4804 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2012 2042 2049 2079 2859 Size: 30 165 30 609 30 7 30 780 Cuts at: 2859 2889 1178 Size: 30 4323 Fragments arranged by size: 4323 780 609 165 30 30 30 30 30 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2020 1186 Size: 834 5200 BseYI C'CCAG_C Cuts at: 2318 2994 3329 5237 5733 2318 Size: 676 335 1908 496 2619 Fragments arranged by size: 2619 1908 676 496 335 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5461 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3299 3484 4245 5273 1540 Size: 275 186 1298 185 761 1028 2301 Fragments arranged by size: 2301 1298 1028 761 275 186 185 BsiHKAI G_wGCw'C Cuts at: 3227 4095 4180 5251 3227 Size: 868 85 1071 4010 Fragments arranged by size: 4010 1071 868 85 BsmI GAATG_Cn' Cuts at: 3545 3638 3545 Size: 93 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2552 2788 2796 3227 3324 3353 4095 Size: 399 762 236 8 431 97 29 742 Cuts at: 4095 4180 5251 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 762 742 431 399 236 97 85 29 8 BspEI T'CCGG_A Cuts at: 5697 5697 Size: 6034 BspHI T'CATG_A Cuts at: 3923 5653 3923 Size: 1730 4304 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2825 3267 3296 3389 844 Size: 1981 442 29 93 3489 Fragments arranged by size: 3489 1981 442 93 29 BsrBI CCG'CTC Cuts at: 1394 1823 3303 3921 1394 Size: 429 1480 618 3507 Fragments arranged by size: 3507 1480 618 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2847 4529 4703 65 Size: 1183 1599 1682 174 1396 Fragments arranged by size: 1682 1599 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3063 4678 4865 1700 Size: 110 1253 1615 187 2869 Fragments arranged by size: 2869 1615 1253 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6034 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6034 BssSI C'ACGA_G Cuts at: 3098 4094 5106 3098 Size: 996 1012 4026 Fragments arranged by size: 4026 1012 996 BstAPI GCAn_nnn'nTGC Cuts at: 794 2606 794 Size: 1812 4222 BstBI TT'CG_AA Cuts at: 4838 4838 Size: 6034 BstEII G'GTnAC_C Cuts at: 1184 3043 1184 Size: 1859 4175 BstYI r'GATC_y Cuts at: 1972 3364 3731 4116 4133 5585 5671 5683 6029 Size: 1392 367 385 17 1452 86 12 346 Cuts at: 6029 1972 Size: 1977 Fragments arranged by size: 1977 1452 1392 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2814 2814 Size: 6034 BtgI C'CryG_G Cuts at: 513 1065 2004 2913 513 Size: 552 939 909 3634 Fragments arranged by size: 3634 939 909 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5295 Fragments arranged by size: 5295 683 56 BtsI GCAGTG_nn' Cuts at: 2147 2520 3082 3550 4344 4364 2147 Size: 373 562 468 794 20 3817 Fragments arranged by size: 3817 794 562 468 373 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6034 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5999 DraI TTT'AAA Cuts at: 3338 3338 Size: 6034 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5041 809 Size: 507 3725 1802 Fragments arranged by size: 3725 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2910 3296 Size: 54 1425 50 275 18 177 903 386 Cuts at: 3296 3481 4370 5999 8 Size: 185 889 1629 43 Fragments arranged by size: 1629 1425 903 889 386 275 185 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3296 3481 1537 Size: 275 1484 185 4090 Fragments arranged by size: 4090 1484 275 185 EarI CTCTTCn'nnn_ Cuts at: 1353 2801 3964 1353 Size: 1448 1163 3423 Fragments arranged by size: 3423 1448 1163 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4621 4995 5141 387 Size: 1064 138 3032 374 146 1280 Fragments arranged by size: 3032 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3225 3225 Size: 6034 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2253 4097 4694 5481 5749 1239 Size: 393 621 1844 597 787 268 1524 Fragments arranged by size: 1844 1524 787 621 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2549 2998 3320 3321 2549 Size: 449 322 1 5262 Fragments arranged by size: 5262 449 322 1 EcoRI G'AATT_C Cuts at: 3343 3721 3343 Size: 378 5656 FspI TGC'GCA Cuts at: 4540 4540 Size: 6034 HaeII r_GCGC'y Cuts at: 1678 3077 5181 1678 Size: 1399 2104 2531 Fragments arranged by size: 2531 2104 1399 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2049 2081 2290 2322 4675 Size: 32 135 32 477 32 209 32 2353 Cuts at: 4675 4707 4749 4781 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2353 477 209 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3378 3624 669 Size: 988 281 1440 246 3079 Fragments arranged by size: 3079 1440 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6034 HpaI GTT'AAC Cuts at: 3624 3624 Size: 6034 KpnI G_GTAC'C Cuts at: 3359 3359 Size: 6034 MfeI C'AATT_G Cuts at: 3633 3633 Size: 6034 MluI A'CGCG_T Cuts at: 5910 5910 Size: 6034 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2845 5148 5332 1305 Size: 1540 2303 184 2007 Fragments arranged by size: 2303 2007 1540 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4212 Fragments arranged by size: 4212 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3992 4351 4510 518 Size: 648 2826 359 159 2042 Fragments arranged by size: 2826 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2194 2528 2680 2909 3265 3450 4127 5275 Size: 588 334 152 229 356 185 677 1148 Cuts at: 5275 5520 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 588 356 334 245 229 185 152 NaeI GCC'GGC Cuts at: 3065 3065 Size: 6034 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4543 Fragments arranged by size: 4543 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6034 NgoMIV G'CCGG_C Cuts at: 3063 3063 Size: 6034 NheI G'CTAG_C Cuts at: 1052 2707 1052 Size: 1655 4379 NotI GC'GGCC_GC Cuts at: 3481 3481 Size: 6034 NspI r_CATG'y Cuts at: 1132 1229 2182 3392 4937 1132 Size: 97 953 1210 1545 2229 Fragments arranged by size: 2229 1545 1210 953 97 PasI CC'CwG_GG Cuts at: 1348 2788 1348 Size: 1440 4594 PciI A'CATG_T Cuts at: 4933 4933 Size: 6034 PflMI CCAn_nnn'nTGG Cuts at: 1108 2859 3088 1108 Size: 1751 229 4054 Fragments arranged by size: 4054 1751 229 PfoI T'CCnGG_A Cuts at: 2740 2740 Size: 6034 PmeI GTTT'AAAC Cuts at: 3338 3338 Size: 6034 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4103 4135 5616 5648 4103 Size: 32 1481 32 4489 Fragments arranged by size: 4489 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6034 PsiI TTA'TAA Cuts at: 3604 3604 Size: 6034 PspOMI G'GGCC_C Cuts at: 2548 3320 2548 Size: 772 5262 PstI C_TGCA'G Cuts at: 830 2527 3248 3386 830 Size: 1697 721 138 3478 Fragments arranged by size: 3478 1697 721 138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6034 PvuII CAG'CTG Cuts at: 2194 2528 2194 Size: 334 5700 SacI G_AGCT'C Cuts at: 3227 3227 Size: 6034 SalI G'TCGA_C Cuts at: 1936 3376 1936 Size: 1440 4594 SapI GCTCTTCn'nnn_ Cuts at: 2801 2801 Size: 6034 SbfI CC_TGCA'GG Cuts at: 3386 3386 Size: 6034 ScaI AGT'ACT Cuts at: 4282 5994 4282 Size: 1712 4322 SfcI C'TryA_G Cuts at: 826 1045 2523 3244 3382 4517 5198 5389 826 Size: 219 1478 721 138 1135 681 191 1471 Fragments arranged by size: 1478 1471 1135 721 681 219 191 138 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6034 SmaI CCC'GGG Cuts at: 1872 3020 3054 3361 1872 Size: 1148 34 307 4545 Fragments arranged by size: 4545 1148 307 34 SmlI C'TyrA_G Cuts at: 820 1017 1943 4138 5039 5301 820 Size: 197 926 2195 901 262 1553 Fragments arranged by size: 2195 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3825 493 Size: 3332 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6034 SphI G_CATG'C Cuts at: 1132 3392 1132 Size: 2260 3774 SspI AAT'ATT Cuts at: 5 52 3958 5 Size: 47 3906 2081 Fragments arranged by size: 3906 2081 47 StuI AGG'CCT Cuts at: 1517 2595 1517 Size: 1078 4956 StyI C'CwwG_G Cuts at: 513 1065 2004 2760 3473 513 Size: 552 939 756 713 3074 Fragments arranged by size: 3074 939 756 713 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3031 3062 4061 4078 4231 4416 4861 Size: 1255 1451 31 999 17 153 185 445 Cuts at: 4861 325 Size: 1498 Fragments arranged by size: 1498 1451 1255 999 445 185 153 31 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4280 5992 96 Size: 274 80 33 51 1443 2303 1712 138 Fragments arranged by size: 2303 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2012 2044 2414 2446 5994 6026 2012 Size: 32 370 32 3548 32 2020 Fragments arranged by size: 3548 2020 370 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6034 XbaI T'CTAG_A Cuts at: 3370 3370 Size: 6034 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2758 2758 Size: 6034 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6034 XmaI C'CCGG_G Cuts at: 1870 3018 3052 3359 1870 Size: 1148 34 307 4545 Fragments arranged by size: 4545 1148 307 34 XmnI GAAnn'nnTTC Cuts at: 4163 4163 Size: 6034 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3842 276 Size: 53 83 186 936 2308 2468 Fragments arranged by size: 2468 2308 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI