(Linear) (Six-base) MAPSORT of: KIEE6304.seq Check: 3971 from: 1 to: 1329 KIEE6304.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1262 1291 1329 Size: 1262 29 38 Fragments arranged by size: 1262 38 29 AccI GT'mk_AC Cuts at: 0 808 1329 Size: 808 521 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 248 1329 Size: 248 1081 AlwNI CAG_nnn'CTG Cuts at: 0 1114 1329 Size: 1114 215 ApaI G_GGCC'C Cuts at: 0 547 1319 1329 Size: 547 772 10 Fragments arranged by size: 772 547 10 ApoI r'AATT_y Cuts at: 0 464 1329 Size: 464 865 AvaI C'yCGr_G Cuts at: 0 1013 1047 1329 Size: 1013 34 282 Fragments arranged by size: 1013 282 34 BanI G'GyrC_C Cuts at: 0 633 1250 1329 Size: 633 617 79 Fragments arranged by size: 633 617 79 BanII G_rGCy'C Cuts at: 0 547 783 791 1222 1319 1329 Size: 547 236 8 431 97 10 Fragments arranged by size: 547 431 236 97 10 8 BbsI GAAGACnn'nnnn_ Cuts at: 0 389 1010 1329 Size: 389 621 319 Fragments arranged by size: 621 389 319 Bme1580I G_kGCm'C Cuts at: 0 547 1319 1329 Size: 547 772 10 Fragments arranged by size: 772 547 10 BmtI G_CTAG'C Cuts at: 0 706 1329 Size: 706 623 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 387 967 1329 Size: 387 580 362 Fragments arranged by size: 580 387 362 Bpu10I CC'TnA_GC Cuts at: 0 985 1329 Size: 985 344 BsaI GGTCTCn'nnnn_ Cuts at: 0 291 1329 Size: 291 1038 BsaAI yAC'GTr Cuts at: 0 1200 1329 Size: 1200 129 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 7 37 44 74 854 884 1329 Size: 7 30 7 30 780 30 445 Fragments arranged by size: 780 445 30 30 30 7 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 15 1329 Size: 15 1314 BseYI C'CCAG_C Cuts at: 0 313 989 1324 1329 Size: 313 676 335 5 Fragments arranged by size: 676 335 313 5 BsiEI CG_ry'CG Cuts at: 0 1294 1329 Size: 1294 35 BsiHKAI G_wGCw'C Cuts at: 0 1222 1329 Size: 1222 107 Bsp1286I G_dGCh'C Cuts at: 0 547 783 791 1222 1319 1329 Size: 547 236 8 431 97 10 Fragments arranged by size: 547 431 236 97 10 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 820 1262 1291 1329 Size: 820 442 29 38 Fragments arranged by size: 820 442 38 29 BsrBI CCG'CTC Cuts at: 0 1298 1329 Size: 1298 31 BsrDI GCAATG_nn' Cuts at: 0 842 1329 Size: 842 487 BsrFI r'CCGG_y Cuts at: 0 1058 1329 Size: 1058 271 BssSI C'ACGA_G Cuts at: 0 1093 1329 Size: 1093 236 BstAPI GCAn_nnn'nTGC Cuts at: 0 601 1329 Size: 601 728 BstEII G'GTnAC_C Cuts at: 0 1038 1329 Size: 1038 291 BstZ17I GTA'TAC Cuts at: 0 809 1329 Size: 809 520 BtgI C'CryG_G Cuts at: 0 908 1329 Size: 908 421 BtsI GCAGTG_nn' Cuts at: 0 142 515 1077 1329 Size: 142 373 562 252 Fragments arranged by size: 562 373 252 142 EaeI y'GGCC_r Cuts at: 0 2 905 1291 1329 Size: 2 903 386 38 Fragments arranged by size: 903 386 38 2 EagI C'GGCC_G Cuts at: 0 1291 1329 Size: 1291 38 EarI CTCTTCn'nnn_ Cuts at: 0 796 1329 Size: 796 533 EcoICRI GAG'CTC Cuts at: 0 1220 1329 Size: 1220 109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 248 1329 Size: 248 1081 EcoO109I rG'GnC_Cy Cuts at: 0 544 993 1315 1316 1329 Size: 544 449 322 1 13 Fragments arranged by size: 544 449 322 13 1 HaeII r_GCGC'y Cuts at: 0 1072 1329 Size: 1072 257 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 44 76 285 317 1329 Size: 44 32 209 32 1012 Fragments arranged by size: 1012 209 44 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 840 1329 Size: 840 489 MspA1I CmG'CkG Cuts at: 0 189 523 675 904 1260 1329 Size: 189 334 152 229 356 69 Fragments arranged by size: 356 334 229 189 152 69 NaeI GCC'GGC Cuts at: 0 1060 1329 Size: 1060 269 NgoMIV G'CCGG_C Cuts at: 0 1058 1329 Size: 1058 271 NheI G'CTAG_C Cuts at: 0 702 1329 Size: 702 627 NspI r_CATG'y Cuts at: 0 177 1329 Size: 177 1152 PasI CC'CwG_GG Cuts at: 0 783 1329 Size: 783 546 PflMI CCAn_nnn'nTGG Cuts at: 0 854 1083 1329 Size: 854 229 246 Fragments arranged by size: 854 246 229 PfoI T'CCnGG_A Cuts at: 0 735 1329 Size: 735 594 PspOMI G'GGCC_C Cuts at: 0 543 1315 1329 Size: 543 772 14 Fragments arranged by size: 772 543 14 PstI C_TGCA'G Cuts at: 0 522 1243 1329 Size: 522 721 86 Fragments arranged by size: 721 522 86 PvuII CAG'CTG Cuts at: 0 189 523 1329 Size: 189 334 806 Fragments arranged by size: 806 334 189 SacI G_AGCT'C Cuts at: 0 1222 1329 Size: 1222 107 SapI GCTCTTCn'nnn_ Cuts at: 0 796 1329 Size: 796 533 SfcI C'TryA_G Cuts at: 0 518 1239 1329 Size: 518 721 90 Fragments arranged by size: 721 518 90 SmaI CCC'GGG Cuts at: 0 1015 1049 1329 Size: 1015 34 280 Fragments arranged by size: 1015 280 34 StuI AGG'CCT Cuts at: 0 590 1329 Size: 590 739 StyI C'CwwG_G Cuts at: 0 755 1329 Size: 755 574 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1026 1057 1329 Size: 1026 31 272 Fragments arranged by size: 1026 272 31 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 7 39 409 441 1329 Size: 7 32 370 32 888 Fragments arranged by size: 888 370 32 32 7 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 753 1329 Size: 753 576 XmaI C'CCGG_G Cuts at: 0 1013 1047 1329 Size: 1013 34 282 Fragments arranged by size: 1013 282 34 Enzymes that do cut: AarI AccI AcuI AlwNI ApaI ApoI AvaI BanI BanII BbsI Bme1580I BmtI BplI Bpu10I BsaI BsaAI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstZ17I BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I MmeI MspA1I NaeI NgoMIV NheI NspI PasI PflMI PfoI PspOMI PstI PvuII SacI SapI SfcI SmaI StuI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BpmI BpuEI BsaBI BsaHI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BstBI BstXI BstYI Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NarI NcoI NdeI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI