################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoT22I FseI FspI HpaI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI XbaI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 172 GTCTAC GT^[AC][GT]AC AcsI 1300 GAATTC [GA]^AATT[CT] AflIII 1082 ACATGT A^C[GA][CT]GT AlwI 1440 GGATC GGATC(4/5) ApaI 498 GGGCCC GGGCC^C ApoI 1300 GAATTC [GA]^AATT[CT] AspI 46 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 746 CTCGAG C^[CT]CG[GA]G BbeI 55 GGCGCC GGCGC^C BbsI 814 GAAGAC GAAGAC(2/6) BclI 1092 TGATCA T^GATCA BcnI 41 CCGGG CC^[GC]GG BglI 289 GCCTTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 814 GAAGAC GAAGAC(2/6) BsgI 425 CTGCAC GTGCAG(16/14) BsmBI 108 CGTCTC CGTCTC(1/5) BsoBI 746 CTCGAG C^[CT]CG[GA]G BspLU11I 1082 ACATGT A^CATGT BsrDI 1311 CATTGC GCAATG(2/0) BsrI 700 ACTGG ACTGG(1/-1) BstAPI 343 GCACAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1149 GGTCACC G^GT[ACGT]ACC BstPI 1149 GGTCACC G^GT[ACGT]ACC EcoO65I 1149 GGTCACC G^GT[ACGT]ACC EcoRI 1300 GAATTC G^AATTC EcoRV 229 GATATC GAT^ATC FauI 60 CCCGC CCCGC(4/6) FbaI 1092 TGATCA T^GATCA HaeII 55 GGCGCC [GA]GCGC^[CT] HapII 41 CCGG C^CGG HincII 1168 GTCAAC GT[CT]^[GA]AC HindII 1168 GTCAAC GT[CT]^[GA]AC HindIII 1073 AAGCTT A^AGCTT HpaII 41 CCGG C^CGG KasI 55 GGCGCC G^GCGCC MaeII 657 ACGT A^CGT MseI 332 TTAA T^TAA MslI 1269 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 41 CCGG C^CGG NarI 55 GGCGCC GG^CGCC NciI 41 CCGGG CC^[GC]GG NspI 1082 ACATGT [GA]CATG^[CT] PaeR7I 746 CTCGAG C^TCGAG PflFI 46 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1543 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 1534 GAGCTC GAGCT^C SfoI 55 GGCGCC GGC^GCC SmlI 746 CTCGAG C^T[CT][GA]AG TaqI 747 TCGA T^CGA Tru9I 332 TTAA T^TAA Tth111I 46 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 747 TCGA T^CGA Van91I 1543 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 746 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccII 62 CGCG CG^CG 106 CGCG AcyI 55 GGCGCC G[GA]^CG[CT]C 304 GACGCC AocI 574 CCTGAGG CC^T[ACGT]AGG 1237 CCTGAGG BanII 498 GGGCCC G[GA]GC[CT]^C 1534 GAGCTC BsaHI 55 GGCGCC G[GA]^CG[CT]C 304 GACGCC BspMI 1229 ACCTGC ACCTGC(4/8) 1495 ACCTGC BstUI 62 CGCG CG^CG 106 CGCG Bsu36I 574 CCTGAGG CC^T[ACGT]AGG 1237 CCTGAGG DsaI 113 CCATGG C^C[GA][CT]GG 1154 CCATGG Eco81I 574 CCTGAGG CC^T[ACGT]AGG 1237 CCTGAGG HgaI 304 GACGC GACGC(5/10) 107 GCGTC Hin1I 55 GGCGCC G[GA]^CG[CT]C 304 GACGCC MspA1I 70 CCGCTG C[AC]G^C[GT]G 989 CAGCGG MvnI 62 CGCG CG^CG 106 CGCG NcoI 113 CCATGG C^CATGG 1154 CCATGG PpuMI 544 GGGTCCC [GA]G^G[AT]CC[CT] 570 AGGACCT SfcI 268 CTACAG C^T[GA][CT]AG 849 CTACAG StuI 248 AGGCCT AGG^CCT 626 AGGCCT Tsp509I 311 AATT ^AATT 1301 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AfaI AluI AlwNI AspHI AvaII BanI BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI SapI Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I TspRI XcmI