(Circular) (Six-base) MAPSORT of: pFN21AE5617.seq Check: 941 from: 1 to: 6343 pFN21AE5617 6343 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4153 278 Size: 53 83 186 936 2617 2468 Fragments arranged by size: 2617 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2178 3686 1937 Size: 241 1508 4594 Fragments arranged by size: 4594 1508 241 Acc65I G'GTAC_C Cuts at: 3664 3664 Size: 6343 AclI AA'CG_TT Cuts at: 1563 4470 4843 1563 Size: 2907 373 3063 Fragments arranged by size: 3063 2907 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2346 2715 2928 3618 4406 5790 6058 1632 Size: 714 369 213 690 788 1384 268 1917 Fragments arranged by size: 1917 1384 788 714 690 369 268 213 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6146 AflIII A'CryG_T Cuts at: 1203 3087 5242 6219 1203 Size: 1884 2155 977 1327 Fragments arranged by size: 2155 1884 1327 977 AgeI A'CCGG_T Cuts at: 5174 5174 Size: 6343 AhdI GACnn_n'nnGTC Cuts at: 5072 5072 Size: 6343 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6343 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6343 AlwNI CAG_nnn'CTG Cuts at: 2579 2738 3454 4086 5658 2579 Size: 159 716 632 1572 3264 Fragments arranged by size: 3264 1572 716 632 159 ApaI G_GGCC'C Cuts at: 2507 2507 Size: 6343 ApaLI G'TGCA_C Cuts at: 4400 5556 4400 Size: 1156 5187 ApoI r'AATT_y Cuts at: 1493 3305 3652 3878 4030 6139 1493 Size: 1812 347 226 152 2109 1697 Fragments arranged by size: 2109 1812 1697 347 226 152 AseI AT'TA_AT Cuts at: 160 1032 4897 160 Size: 872 3865 1606 Fragments arranged by size: 3865 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6343 AvaI C'yCGr_G Cuts at: 1870 1943 2751 3668 1870 Size: 73 808 917 4545 Fragments arranged by size: 4545 917 808 73 BamHI G'GATC_C Cuts at: 3673 4040 3673 Size: 367 5976 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2009 2060 2944 3457 3664 Size: 325 217 627 222 51 884 513 207 Cuts at: 3664 5119 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 884 627 513 325 222 217 207 51 BanII G_rGCy'C Cuts at: 1391 2507 3543 3662 1391 Size: 1116 1036 119 4072 Fragments arranged by size: 4072 1116 1036 119 BbeI G_GCGC'C Cuts at: 2064 2064 Size: 6343 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2826 928 Size: 971 927 4445 Fragments arranged by size: 4445 971 927 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4534 4568 1903 Size: 34 2597 34 3678 Fragments arranged by size: 3678 2597 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4235 5445 4235 Size: 1210 5133 BclI T'GATC_A Cuts at: 1548 3097 1548 Size: 1549 4794 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2300 4954 136 Size: 107 122 71 631 605 628 2654 1525 Fragments arranged by size: 2654 1525 631 628 605 122 107 71 BglII A'GATC_T Cuts at: 6338 6338 Size: 6343 BlpI GC'TnA_GC Cuts at: 3760 3760 Size: 6343 Bme1580I G_kGCm'C Cuts at: 1790 2507 4404 5560 1790 Size: 717 1897 1156 2573 Fragments arranged by size: 2573 1897 1156 717 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6343 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4074 5032 442 Size: 416 53 83 399 24 2657 958 1753 Fragments arranged by size: 2657 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6343 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2992 3598 2992 Size: 606 5737 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2544 3178 3527 5003 1239 Size: 1305 634 349 1476 2579 Fragments arranged by size: 2579 1476 1305 634 349 Bpu10I CC'TnA_GC Cuts at: 2145 2145 Size: 6343 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4468 5333 5631 4468 Size: 865 298 5180 Fragments arranged by size: 5180 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5006 882 Size: 617 3507 2219 Fragments arranged by size: 3507 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4134 493 Size: 711 2930 2702 Fragments arranged by size: 2930 2702 711 BsaBI GATnn'nnATC Cuts at: 6337 6337 Size: 6343 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 2310 4150 4532 Size: 53 83 186 936 528 249 1840 382 Cuts at: 4532 5167 275 Size: 635 1451 Fragments arranged by size: 1840 1451 936 635 528 382 249 186 83 53 BsaWI w'CCGG_w Cuts at: 4776 5174 5448 5595 6006 4776 Size: 398 274 147 411 5113 Fragments arranged by size: 5113 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2075 2105 3398 3428 1178 Size: 30 165 30 672 30 1293 30 4093 Fragments arranged by size: 4093 1293 672 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2438 2879 2915 2981 3587 1186 Size: 1252 441 36 66 606 3942 Fragments arranged by size: 3942 1252 606 441 66 36 BseYI C'CCAG_C Cuts at: 2022 2714 5546 6042 2022 Size: 692 2832 496 2323 Fragments arranged by size: 2832 2323 692 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2415 1163 Size: 573 679 5091 Fragments arranged by size: 5091 679 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3793 4554 5582 1540 Size: 275 186 1792 761 1028 2301 Fragments arranged by size: 2301 1792 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2045 3543 3582 4404 4489 5560 2045 Size: 1498 39 822 85 1071 2828 Fragments arranged by size: 2828 1498 1071 822 85 39 BsmI GAATG_Cn' Cuts at: 2697 2837 3145 3854 3947 2697 Size: 140 308 709 93 5093 Fragments arranged by size: 5093 709 308 140 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2119 3633 2119 Size: 1514 4829 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2507 3543 3582 3662 4404 4489 Size: 399 255 462 1036 39 80 742 85 Cuts at: 4489 5560 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1036 742 462 399 255 85 80 39 BspEI T'CCGG_A Cuts at: 6006 6006 Size: 6343 BspHI T'CATG_A Cuts at: 4232 5962 4232 Size: 1730 4613 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3243 3509 3698 844 Size: 2399 266 189 3489 Fragments arranged by size: 3489 2399 266 189 BsrBI CCG'CTC Cuts at: 1394 1823 4230 1394 Size: 429 2407 3507 Fragments arranged by size: 3507 2407 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3315 4838 5012 65 Size: 1183 2067 1523 174 1396 Fragments arranged by size: 2067 1523 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4987 5174 1700 Size: 110 3177 187 2869 Fragments arranged by size: 3177 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6343 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6343 BssSI C'ACGA_G Cuts at: 4403 5415 4403 Size: 1012 5331 BstAPI GCAn_nnn'nTGC Cuts at: 794 2354 794 Size: 1560 4783 BstBI TT'CG_AA Cuts at: 5147 5147 Size: 6343 BstEII G'GTnAC_C Cuts at: 1184 3154 1184 Size: 1970 4373 BstYI r'GATC_y Cuts at: 1972 3673 4040 4425 4442 5894 5980 5992 6338 Size: 1701 367 385 17 1452 86 12 346 Cuts at: 6338 1972 Size: 1977 Fragments arranged by size: 1977 1701 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2580 3243 2580 Size: 663 5680 BtgI C'CryG_G Cuts at: 513 1065 2004 2118 3159 513 Size: 552 939 114 1041 3697 Fragments arranged by size: 3697 1041 939 552 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5604 Fragments arranged by size: 5604 683 56 BtsI GCAGTG_nn' Cuts at: 2432 2866 3859 4653 4673 2432 Size: 434 993 794 20 4102 Fragments arranged by size: 4102 993 794 434 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6343 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2979 3014 529 Size: 35 2415 35 3858 Fragments arranged by size: 3858 2415 35 35 DraI TTT'AAA Cuts at: 3647 3647 Size: 6343 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5350 809 Size: 507 4034 1802 Fragments arranged by size: 4034 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3790 4679 6308 Size: 54 1425 50 275 18 1960 889 1629 Cuts at: 6308 8 Size: 43 Fragments arranged by size: 1960 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3790 1537 Size: 275 1978 4090 Fragments arranged by size: 4090 1978 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2439 2460 2734 2808 2838 2904 2928 3429 Size: 1086 21 274 74 30 66 24 501 Cuts at: 3429 4273 1353 Size: 844 3423 Fragments arranged by size: 3423 1086 844 501 274 74 66 30 24 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2083 2819 4930 5304 5450 387 Size: 1064 138 494 736 2111 374 146 1280 Fragments arranged by size: 2111 1280 1064 736 494 374 146 138 EcoICRI GAG'CTC Cuts at: 3541 3541 Size: 6343 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2346 2544 2715 2928 3178 3527 3618 Size: 393 714 198 171 213 250 349 91 Cuts at: 3618 4406 5003 5790 6058 1239 Size: 788 597 787 268 1524 Fragments arranged by size: 1524 788 787 714 597 393 349 268 250 213 198 171 91 EcoO109I rG'GnC_Cy Cuts at: 2503 2504 2550 2557 2576 3181 2503 Size: 1 46 7 19 605 5665 Fragments arranged by size: 5665 605 46 19 7 1 EcoRI G'AATT_C Cuts at: 3305 3652 4030 3305 Size: 347 378 5618 Fragments arranged by size: 5618 378 347 EcoRV GAT'ATC Cuts at: 2236 2236 Size: 6343 FspI TGC'GCA Cuts at: 4849 4849 Size: 6343 HaeII r_GCGC'y Cuts at: 1678 2064 5490 1678 Size: 386 3426 2531 Fragments arranged by size: 3426 2531 386 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2777 2809 4984 5016 5058 Size: 32 135 32 1205 32 2175 32 42 Cuts at: 5058 5090 1373 Size: 32 2626 Fragments arranged by size: 2626 2175 1205 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3175 3687 3933 669 Size: 988 281 1237 512 246 3079 Fragments arranged by size: 3079 1237 988 512 281 246 HindIII A'AGCT_T Cuts at: 748 3078 748 Size: 2330 4013 HpaI GTT'AAC Cuts at: 3933 3933 Size: 6343 KasI G'GCGC_C Cuts at: 2060 2060 Size: 6343 KpnI G_GTAC'C Cuts at: 3668 3668 Size: 6343 MfeI C'AATT_G Cuts at: 3942 3942 Size: 6343 MluI A'CGCG_T Cuts at: 6219 6219 Size: 6343 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2676 5457 5641 1305 Size: 1371 2781 184 2007 Fragments arranged by size: 2781 2007 1371 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4521 Fragments arranged by size: 4521 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3278 4301 4660 4819 518 Size: 648 2112 1023 359 159 2042 Fragments arranged by size: 2112 2042 1023 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2077 2996 3759 4436 5584 5829 1606 Size: 471 919 763 677 1148 245 2120 Fragments arranged by size: 2120 1148 919 763 677 471 245 NarI GG'CG_CC Cuts at: 2061 2061 Size: 6343 NcoI C'CATG_G Cuts at: 513 1065 2004 2118 3159 513 Size: 552 939 114 1041 3697 Fragments arranged by size: 3697 1041 939 552 114 NdeI CA'TA_TG Cuts at: 387 387 Size: 6343 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6343 NotI GC'GGCC_GC Cuts at: 3790 3790 Size: 6343 NspI r_CATG'y Cuts at: 1132 1229 3091 3701 5246 1132 Size: 97 1862 610 1545 2229 Fragments arranged by size: 2229 1862 1545 610 97 PasI CC'CwG_GG Cuts at: 1348 2554 1348 Size: 1206 5137 PciI A'CATG_T Cuts at: 3087 5242 3087 Size: 2155 4188 PflMI CCAn_nnn'nTGG Cuts at: 1108 3554 1108 Size: 2446 3897 PmeI GTTT'AAAC Cuts at: 3647 3647 Size: 6343 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4412 4444 5925 5957 4412 Size: 32 1481 32 4798 Fragments arranged by size: 4798 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2550 2576 2550 Size: 26 6317 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6343 PsiI TTA'TAA Cuts at: 3913 3913 Size: 6343 PspOMI G'GGCC_C Cuts at: 2503 2503 Size: 6343 PspXI vC'TCGA_Gb Cuts at: 2751 2751 Size: 6343 PstI C_TGCA'G Cuts at: 830 3695 830 Size: 2865 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6343 SacI G_AGCT'C Cuts at: 3543 3543 Size: 6343 SalI G'TCGA_C Cuts at: 1936 3685 1936 Size: 1749 4594 SanDI GG'GwC_CC Cuts at: 2550 2550 Size: 6343 SapI GCTCTTCn'nnn_ Cuts at: 2460 2734 3429 2460 Size: 274 695 5374 Fragments arranged by size: 5374 695 274 SbfI CC_TGCA'GG Cuts at: 3695 3695 Size: 6343 ScaI AGT'ACT Cuts at: 4591 6303 4591 Size: 1712 4631 SfcI C'TryA_G Cuts at: 826 1045 2273 2854 3691 4826 5507 5698 826 Size: 219 1228 581 837 1135 681 191 1471 Fragments arranged by size: 1471 1228 1135 837 681 581 219 191 SfoI GGC'GCC Cuts at: 2062 2062 Size: 6343 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6343 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6343 SmaI CCC'GGG Cuts at: 1872 3670 1872 Size: 1798 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2751 4447 5348 5610 820 Size: 197 926 808 1696 901 262 1553 Fragments arranged by size: 1696 1553 926 901 808 262 197 SnaBI TAC'GTA Cuts at: 493 4134 493 Size: 3641 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6343 SphI G_CATG'C Cuts at: 1132 3701 1132 Size: 2569 3774 SspI AAT'ATT Cuts at: 5 52 4267 5 Size: 47 4215 2081 Fragments arranged by size: 4215 2081 47 StuI AGG'CCT Cuts at: 1517 2255 2633 1517 Size: 738 378 5227 Fragments arranged by size: 5227 738 378 StyI C'CwwG_G Cuts at: 513 1065 2004 2118 2628 3159 3782 513 Size: 552 939 114 510 531 623 3074 Fragments arranged by size: 3074 939 623 552 531 510 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 4370 4387 4540 4725 5170 325 Size: 1255 417 2373 17 153 185 445 1498 Fragments arranged by size: 2373 1498 1255 445 417 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2211 4589 6301 Size: 274 80 33 51 1443 234 2378 1712 Cuts at: 6301 96 Size: 138 Fragments arranged by size: 2378 1712 1443 274 234 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6303 6335 6303 Size: 32 6311 Tth111I GACn'n_nGTC Cuts at: 1316 2054 1316 Size: 738 5605 XbaI T'CTAG_A Cuts at: 3679 3679 Size: 6343 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2519 2561 3551 2519 Size: 42 990 5311 Fragments arranged by size: 5311 990 42 XhoI C'TCGA_G Cuts at: 1943 2751 1943 Size: 808 5535 XmaI C'CCGG_G Cuts at: 1870 3668 1870 Size: 1798 4545 XmnI GAAnn'nnTTC Cuts at: 4472 4472 Size: 6343 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4151 276 Size: 53 83 186 936 2617 2468 Fragments arranged by size: 2617 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI PvuII RsrII SacII SexAI SfiI SrfI SwaI