(Linear) (Six-base) MAPSORT of: KIEE5617.seq Check: 5061 from: 1 to: 1638 KIEE5617.orf With 182 enzymes: SgfI * December 5, 2014 13:03 .. AccI GT'mk_AC Cuts at: 0 173 1638 Size: 173 1465 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 341 710 923 1613 1638 Size: 341 369 213 690 25 Fragments arranged by size: 690 369 341 213 25 AflIII A'CryG_T Cuts at: 0 1082 1638 Size: 1082 556 AlwNI CAG_nnn'CTG Cuts at: 0 574 733 1449 1638 Size: 574 159 716 189 Fragments arranged by size: 716 574 189 159 ApaI G_GGCC'C Cuts at: 0 502 1638 Size: 502 1136 ApoI r'AATT_y Cuts at: 0 1300 1638 Size: 1300 338 AvaI C'yCGr_G Cuts at: 0 746 1638 Size: 746 892 BanI G'GyrC_C Cuts at: 0 4 55 939 1452 1638 Size: 4 51 884 513 186 Fragments arranged by size: 884 513 186 51 4 BanII G_rGCy'C Cuts at: 0 502 1538 1638 Size: 502 1036 100 Fragments arranged by size: 1036 502 100 BbeI G_GCGC'C Cuts at: 0 59 1638 Size: 59 1579 BbsI GAAGACnn'nnnn_ Cuts at: 0 821 1638 Size: 821 817 BclI T'GATC_A Cuts at: 0 1092 1638 Size: 1092 546 BglI GCCn_nnn'nGGC Cuts at: 0 295 1638 Size: 295 1343 Bme1580I G_kGCm'C Cuts at: 0 502 1638 Size: 502 1136 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 987 1593 1638 Size: 987 606 45 Fragments arranged by size: 987 606 45 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 539 1173 1522 1638 Size: 539 634 349 116 Fragments arranged by size: 634 539 349 116 Bpu10I CC'TnA_GC Cuts at: 0 140 1638 Size: 140 1498 BsaHI Gr'CG_yC Cuts at: 0 56 305 1638 Size: 56 249 1333 Fragments arranged by size: 1333 249 56 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 70 100 1393 1423 1638 Size: 70 30 1293 30 215 Fragments arranged by size: 1293 215 70 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 433 874 910 976 1582 1638 Size: 433 441 36 66 606 56 Fragments arranged by size: 606 441 433 66 56 36 BseYI C'CCAG_C Cuts at: 0 17 709 1638 Size: 17 692 929 Fragments arranged by size: 929 692 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 410 1638 Size: 410 1228 BsiHKAI G_wGCw'C Cuts at: 0 40 1538 1577 1638 Size: 40 1498 39 61 Fragments arranged by size: 1498 61 40 39 BsmI GAATG_Cn' Cuts at: 0 692 832 1140 1638 Size: 692 140 308 498 Fragments arranged by size: 692 498 308 140 BsmBI CGTCTCn'nnnn_ Cuts at: 0 114 1638 Size: 114 1524 Bsp1286I G_dGCh'C Cuts at: 0 40 502 1538 1577 1638 Size: 40 462 1036 39 61 Fragments arranged by size: 1036 462 61 40 39 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1238 1504 1638 Size: 1238 266 134 Fragments arranged by size: 1238 266 134 BsrDI GCAATG_nn' Cuts at: 0 1310 1638 Size: 1310 328 BstAPI GCAn_nnn'nTGC Cuts at: 0 349 1638 Size: 349 1289 BstEII G'GTnAC_C Cuts at: 0 1149 1638 Size: 1149 489 Bsu36I CC'TnA_GG Cuts at: 0 575 1238 1638 Size: 575 663 400 Fragments arranged by size: 663 575 400 BtgI C'CryG_G Cuts at: 0 113 1154 1638 Size: 113 1041 484 Fragments arranged by size: 1041 484 113 BtsI GCAGTG_nn' Cuts at: 0 427 861 1638 Size: 427 434 777 Fragments arranged by size: 777 434 427 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 974 1009 1638 Size: 974 35 629 Fragments arranged by size: 974 629 35 EarI CTCTTCn'nnn_ Cuts at: 0 434 455 729 803 833 899 923 1424 Size: 434 21 274 74 30 66 24 501 Cuts at: 1424 1638 Size: 214 Fragments arranged by size: 501 434 274 214 74 66 30 24 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 78 814 1638 Size: 78 736 824 Fragments arranged by size: 824 736 78 EcoICRI GAG'CTC Cuts at: 0 1536 1638 Size: 1536 102 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 341 539 710 923 1173 1522 1613 1638 Size: 341 198 171 213 250 349 91 25 Fragments arranged by size: 349 341 250 213 198 171 91 25 EcoO109I rG'GnC_Cy Cuts at: 0 498 499 545 552 571 1176 1638 Size: 498 1 46 7 19 605 462 Fragments arranged by size: 605 498 462 46 19 7 1 EcoRI G'AATT_C Cuts at: 0 1300 1638 Size: 1300 338 EcoRV GAT'ATC Cuts at: 0 231 1638 Size: 231 1407 HaeII r_GCGC'y Cuts at: 0 59 1638 Size: 59 1579 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 772 804 1638 Size: 772 32 834 Fragments arranged by size: 834 772 32 HincII GTy'rAC Cuts at: 0 1170 1638 Size: 1170 468 HindIII A'AGCT_T Cuts at: 0 1073 1638 Size: 1073 565 KasI G'GCGC_C Cuts at: 0 55 1638 Size: 55 1583 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 671 1638 Size: 671 967 MslI CAynn'nnrTG Cuts at: 0 1273 1638 Size: 1273 365 MspA1I CmG'CkG Cuts at: 0 72 991 1638 Size: 72 919 647 Fragments arranged by size: 919 647 72 NarI GG'CG_CC Cuts at: 0 56 1638 Size: 56 1582 NcoI C'CATG_G Cuts at: 0 113 1154 1638 Size: 113 1041 484 Fragments arranged by size: 1041 484 113 NspI r_CATG'y Cuts at: 0 1086 1638 Size: 1086 552 PasI CC'CwG_GG Cuts at: 0 549 1638 Size: 549 1089 PciI A'CATG_T Cuts at: 0 1082 1638 Size: 1082 556 PflMI CCAn_nnn'nTGG Cuts at: 0 1549 1638 Size: 1549 89 PpuMI rG'GwC_Cy Cuts at: 0 545 571 1638 Size: 545 26 1067 Fragments arranged by size: 1067 545 26 PspOMI G'GGCC_C Cuts at: 0 498 1638 Size: 498 1140 PspXI vC'TCGA_Gb Cuts at: 0 746 1638 Size: 746 892 SacI G_AGCT'C Cuts at: 0 1538 1638 Size: 1538 100 SanDI GG'GwC_CC Cuts at: 0 545 1638 Size: 545 1093 SapI GCTCTTCn'nnn_ Cuts at: 0 455 729 1424 1638 Size: 455 274 695 214 Fragments arranged by size: 695 455 274 214 SfcI C'TryA_G Cuts at: 0 268 849 1638 Size: 268 581 789 Fragments arranged by size: 789 581 268 SfoI GGC'GCC Cuts at: 0 57 1638 Size: 57 1581 SmlI C'TyrA_G Cuts at: 0 746 1638 Size: 746 892 StuI AGG'CCT Cuts at: 0 250 628 1638 Size: 250 378 1010 Fragments arranged by size: 1010 378 250 StyI C'CwwG_G Cuts at: 0 113 623 1154 1638 Size: 113 510 531 484 Fragments arranged by size: 531 510 484 113 TatI w'GTAC_w Cuts at: 0 206 1638 Size: 206 1432 Tth111I GACn'n_nGTC Cuts at: 0 49 1638 Size: 49 1589 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 514 556 1546 1638 Size: 514 42 990 92 Fragments arranged by size: 990 514 92 42 XhoI C'TCGA_G Cuts at: 0 746 1638 Size: 746 892 Enzymes that do cut: AccI AcuI AflIII AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BclI BglI Bme1580I BplI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BstAPI BstEII Bsu36I BtgI BtsI CspCI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I HincII HindIII KasI MmeI MslI MspA1I NarI NcoI NspI PasI PciI PflMI PpuMI PspOMI PspXI SacI SanDI SapI SfcI SfoI SmlI StuI StyI TatI Tth111I XcmI XhoI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I BtgZI ClaI DraI DraIII DrdI EaeI EagI EcoNI FalI FseI FspI FspAI HpaI KpnI MfeI MluI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI XbaI XmaI XmnI ZraI